Template-Type: ReDIF-Article 1.0 Author-Name: M.R. Alam Author-Workplace-Name: Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh Author-Name: W.I. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea Author-Name: J.W. Kim Author-Workplace-Name: College of Medical Science, Seonam University, Namwon, Korea Author-Name: C.S. Na Author-Workplace-Name: College of Agriculture and Life Science, Chonbuk National University, Jeonju, Korea Author-Name: N.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea Title: Effects of Chitosan-oligosaccharide on diarrhoea in Hanwoo calves Abstract: The therapeutic effect of Chitosan-oligosaccharide (COS) on calf diarrhoea was investigated in Korean native (Hanwoo) calves (n = 86). The calves were divided into two groups; the untreated/control (n = 40) having routine diet only and the treated group (n = 46) receiving COS (50 ml/day) in addition to the routine diet for five days. Blood samples from each animal were collected before and after five days treatment and were subjected to complete blood count (CBC), chemistry screening (CS) and plasma protein assay (PPA). The in vitro efficacy of COS against the most common causal agents of calf diarrhoea (Escherichia coli and Salmonella typhimurium) was evaluated. Among the CBC parameters a significantly higher total red blood cell count (tRBC), haemoglobin (Hb) and packed cell volume (PCV) were noticed in the untreated group. In the CS parameters higher levels of blood urea nitrogen (BUN), creatinine and total bilirubin (TBL), and lower glucose were found in the untreated group. Significantly higher levels of albumin and α-globulin along with lower γ-globulin were noted in the untreated group. Among the treated group 41 (out of 46) calves recovered completely from diarrhoea and in the untreated group five calves (out of 40) spontaneously recovered after five days, revealing that the COS has a good therapeutic effect on diarrhoea in calves. The COS was found to effectively inhibit bacterial growth and pathogenicity up to 1:64 and 1:256 dilutions in the case of S. typhimurium and E. coli respectively. The results of this study revealed that the levels of different parameters of CBC (tRBC, Hb, PCV), CS (BUN, creatinine, TBL) and PPA (albumin, α-globulin, γ-globulin) profiles can reflect the severity of diarrhoea and dehydration and that COS can be successfully used for the clinical management of diarrhoea in Hanwoo calves. Keywords: Chitosan, complete blood count, chemistry screening, plasma proteins, diarrhoea, calf Journal: Veterinární medicína Pages: 385-393 Volume: 57 Issue: 8 Year: 2012 DOI: 10.17221/6306-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6306-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201208-0001.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:8:id:6306-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Pechova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: L. Sevcikova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: L. Pavlata Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: R. Dvorak Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: The effect of various forms of selenium supplied to pregnant goats on selected blood parameters and on the concentration of Se in urine and blood of kids at the time of weaning Abstract: The aim of this trial was to compare the effect of supplementation of goats with different forms of selenium on the metabolism of their kids at the time of weaning. The experiment was performed with 45 kids of mothers supplemented with various forms of selenium. Group C was control while the other four groups were supplemented with selenium for six weeks before (0.3 mg/goat/day) and after parturition (0.9 mg/goat/day). Group Se-I received sodium selenite while the other groups received organic forms: Se-lactate-protein complex (Se-L), Se-proteinate (Se-P) and Se-yeast (Se-Y). The kids were weaned at three months of age and samples of blood and urine were taken. Parameters monitored in the blood included Se, glutathione peroxidase (GPx), Zn, Cu, thyroxine, triiodothyronine, protein, immunoglobulins, muscle enzymes, total antioxidant status, vitamin A and E. Se levels were determined In the urine. Selenium supplementation of goats from six weeks before delivery significantly influenced selenium concentrations in the blood of kids. Significant differences (P < 0.0002) were found between the control and all experimental groups and further between Se-Y and the other experimental groups (Se-Y: 243.0 ± 20.3 μg/l; Se-I: 156.3 ± 34.3 μg/l; Se-P: 152.6 ± 41.5 μg/l; Se-L: 146.7 ± 20 0 μg/l; C: 67.6 ± 13.1 μg/l). The highest concentration was found in the group supplemented with Se-yeast with a high content of selenomethionine. The other two organic forms of selenium (proteinate and lactate-protein complex) increased the concentration of Se in blood and the activity of GPx to the same extent as the inorganic form of selenium. Se supplementation did not have a negative effect on the concentration of copper and zinc in the blood serum of kids, but we found decreased concentrations of thyroxine in the experimental groups (Se-Y: 79.8 ± 12.8 nmol/l; Se-I: 66.5 ± 13.2 nmol/l; Se-P: 76.2 ± 25.7 nmol/l; Se-L: 84.5 ± 14.8 nmol/l; C: 92.7 ± 13.4 nmol/l). Significant differences were found between the group C and groups Se-I and Se-P (P < 0.05). The supplementation of mothers with Se both in organic and inorganic forms was sufficient to prevent Se deficiency in kids at the time of weaning. Keywords: ruminants, Se-yeast, sodium selenite, Se-lactate, Se-proteinate, Se-lactate-protein complex, zinc, copper, thyroxine, creatine kinase, glutathione peroxidase Journal: Veterinární medicína Pages: 394-403 Volume: 57 Issue: 8 Year: 2012 DOI: 10.17221/6307-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6307-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201208-0002.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:8:id:6307-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B. Mobini Author-Workplace-Name: College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch Shahrekord, Iran Title: Histological and histochemical studies on the Harderian gland in native chickens Abstract: The objective of this investigation was to study the histological and histochemical structure of the Harderian gland in native chickens. Samples were obtained from 10 male and 10 female adult healthy native chickens. Tissue sections were stained with haematoxylin eosin, Verhoeff's, Masson's trichrome, alcian blue (pH 2.5), periodic acid-Schiff and Gomori's method for reticulum. The multilobular Harderian gland of native chickens was covered by a thin connective tissue which consisted of adipose tissue, parasympathetic ganglia, nerve bundles, collagen, elastic and reticular fibres. Plasma cells were present in interlobular areas. The Harderian gland was compound tubulo-alveolar type. The Harderian duct was lined by columnar epithelial cells of varying height. Goblet cells were not found in Harderian duct. Histochemical staining revealed that the all epithelial cells of both corpus glandulae and ducts contained both neutral and acidic mucins. No significant sex-based differences were found. It is concluded that the general histological and histochemical structure of the Harderian gland in native chickens is similar to that of domestic geese, but that there are also some differences. Keywords: Harderian gland, histochemical, histological, native chicken Journal: Veterinární medicína Pages: 404-409 Volume: 57 Issue: 8 Year: 2012 DOI: 10.17221/6308-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6308-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201208-0003.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:8:id:6308-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: G. Zur Author-Workplace-Name: The Veterinary Teaching Hospital, Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel Author-Name: I. Skorinsky Author-Workplace-Name: Monosson VetDerm Referrals, Monosson, Israel Author-Name: T. Bdolah-Abram Author-Workplace-Name: The Veterinary Teaching Hospital, Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel Title: Canine atopic dermatitis in the Middle East: clinical signs, signalment and common allergens Abstract: The aim of the study was to examine for the first time the various aspects of canine atopic dermatitis (CAD) presenting in a Middle Eastern country. Medical records of 164 dogs diagnosed with CAD were evaluated. Associations between signalment, lifestyle, clinical signs and allergens were evaluated statistically. Labrador Retriever, German Shepherd dog, Boxer, French Bulldog, Golden Retriever and Shar-Pei breeds were presented more frequently than the regular hospital population (P < 0.0001), and had an earlier disease onset time (P < 0.01). In 22 dogs (13%) signs of CAD were noticed at less than six months of age. Most dogs (75%) lived indoors. Most dogs (83%) had lesions on the ventral part of the body and 68% had foot lesions. After excluding flea allergy dermatitis by implementing strict flea control, 60% of the dogs presented with dorsal distribution of skin lesions. Dogs with ventral lesions were younger when clinical signs first appeared (P < 0.05). Most of the dogs were allergic to dust and/or dust mites (75.6%), with weeds and trees as the next most common allergens. CAD is similar worldwide, but geographic differences may be attributable to genetic pools and allergen loading. This study also shows that early onset of clinical signs, especially in breeds predisposed to CAD and with a dorsal distribution of skin lesions, should not rule out the diagnosis of CAD. Keywords: dog, allergies, skin lesions, otitis externa Journal: Veterinární medicína Pages: 410-419 Volume: 57 Issue: 8 Year: 2012 DOI: 10.17221/6309-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6309-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201208-0004.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:8:id:6309-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Demirutku Author-Workplace-Name: Veterinary Faculty, Istanbul University, Istanbul, Turkey Author-Name: K. Ozer Author-Workplace-Name: Veterinary Faculty, Istanbul University, Istanbul, Turkey Author-Name: Y. Devecioglu Author-Workplace-Name: Veterinary Faculty, Istanbul University, Istanbul, Turkey Author-Name: Z. Mutlu Author-Workplace-Name: Veterinary Faculty, Istanbul University, Istanbul, Turkey Author-Name: O. Duzgun Author-Workplace-Name: Veterinary Faculty, Istanbul University, Istanbul, Turkey Author-Name: E. Eravci Author-Workplace-Name: Veterinary Faculty, Istanbul University, Istanbul, Turkey Author-Name: D. Haktanir Author-Workplace-Name: Veterinary Faculty, Istanbul University, Istanbul, Turkey Author-Name: S.S. Arun Author-Workplace-Name: Veterinary Faculty, Istanbul University, Istanbul, Turkey Title: Pinnal squamous cell carcinoma in cats and the effectiveness of treatment with radical pinnectomy Abstract: The aim of this study was to observe the effectiveness of treatment with radical pinnectomy and investigate postoperative relapse cases in pinnal squamous cell carcinoma in cats. Twelve cats which were brought to surgery clinic of Istanbul University, Faculty of Veterinary Medicine were used. Biopsies were taken from the cases in which squamous cell carcinoma was suspected following anamnesis and the clinical examination of patients that were brought to our clinic. The patients were diagnosed on the basis of histopathological analysis of biopsy samples. Haemogram and biochemical blood analysis were performed and thoracic radiographs were taken as routine in cases that a decision was made to operate. After the area was prepared for operation radical pinnectomy or total external acoustic canal ablation along with radical pinnectomy were performed in the patients that were eligible for operation. Relapses seen in the postoperative period were re-operated with the techniques selected according to invasion status. Periodic controls of the patients were made after the operations and the cats were examined for relapse. In seven out of 12 cases, only squamous cell carcinoma; in three cases, actinic dermatitis actinic dermatitis in addition to squamous cell carcinoma, and in two cases, only actinic dermatitis lesions were detected. In the postoperative period, relapse was observed in the cases in which the squamous cell carcinoma lesion reached the lower half of the pinna or the external acoustic canal while relapse was not observed in cases with actinic dermatitis lesions or when the squamous cell carcinoma lesion was on the top part of the pinna. Relapse cases were re-operated using different operative techniques. It is concluded that performing radical pinnectomy on actinic dermatitis lesions detected on the pinna before development into squamous cell carcinoma, and removing the tumour according to its localization with radical pinnectomy or total external acoustic canal ablation alongside radical pinnectomy is the most reliable treatment option in terms of relapse in cases of pinnal squamous cell carcinoma. Keywords: ear, pinna, actinic dermatitis, squamous cell carcinoma, radical pinnectomy, cat Journal: Veterinární medicína Pages: 420-429 Volume: 57 Issue: 8 Year: 2012 DOI: 10.17221/6310-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6310-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201208-0005.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:8:id:6310-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Aydin Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: M. Karan Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Title: The spinal nerves forming the brachial plexus in mole-rats (Spalax leucodon) Abstract: In this study, the brachial plexus in the mole-rat was investigated. Six adult mole-rats were used and the brachial plexus of each was dissected. It was found that the brachial plexus in the mole-rat was formed by the rami ventralis of C5. A single root was formed from a major part of the ramus ventralis of C5 and through the joining of the whole of the ramus ventralis of C6, C7, C8 and T1. Thus, in mole-rats, the spinal nerves forming the brachial plexus and the joining of these spinal nerves to each other differ from other rodents and mammals. Keywords: spinal nerves, rami ventralis, brachial plexus, the mole-rats (Spalax leucodon) Journal: Veterinární medicína Pages: 430-433 Volume: 57 Issue: 8 Year: 2012 DOI: 10.17221/6311-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6311-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201208-0006.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:8:id:6311-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Garcia-Sancho Author-Workplace-Name: College of Veterinary Medicine, Complutense University of Madrid, Spain Author-Name: F. Rodriguez-Franco Author-Workplace-Name: College of Veterinary Medicine, Complutense University of Madrid, Spain Author-Name: A. Rodriguez-Bertos Author-Workplace-Name: College of Veterinary Medicine, Complutense University of Madrid, Spain Author-Name: V. Carrasco Author-Workplace-Name: College of Veterinary Medicine, Complutense University of Madrid, Spain Author-Name: A. Villaescusa Author-Workplace-Name: College of Veterinary Medicine, Complutense University of Madrid, Spain Author-Name: A. Sainz Author-Workplace-Name: College of Veterinary Medicine, Complutense University of Madrid, Spain Title: Transient ileocolic intussusception in a dog with histiocytic ulcerative tiflocolitis: a case report Abstract: In human medicine, the association between intussusceptions and inflammatory bowel disease is well known, even if referred to only in case reports or a small series of patients. The exact cause of intussusceptions, in general, is unknown; however, any lesion in the bowel wall or irritant in the lumen that alters the normal peristaltic pattern may initiate invagination. In Crohn's disease the lead point may be dysrhytmic contractions secondary to the on-going inflammatory process of a thickened, inflamed segment of bowel wall. In veterinary medicine, the information available related to the association of intussusceptions and inflammatory bowel disease is limited. The present study is the first reported case of a dog in which ileocolic intussusception and histiocytic ulcerative colitis appear to be associated. Moreover, this is also the first description of the involvement of the caecum in canine histiocytic ulcerative colitis. Herein, we document a condition previously reported in humans with inflammatory bowel disease, and we believe that this work contributes to identifying the similarities between human and canine inflammatory bowel disease. Keywords: inflammatory bowel disease, canine, invagination Journal: Veterinární medicína Pages: 434-438 Volume: 57 Issue: 8 Year: 2012 DOI: 10.17221/6312-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6312-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201208-0007.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:8:id:6312-VETMED