Template-Type: ReDIF-Article 1.0 Author-Name: D.V.A. Khoa Author-Workplace-Name: College of Agriculture and Applied Biology, Can Tho University, Vietnam Title: Structural homology and expression tendency of the natural immune response of the terminal complement components to inoculations in pigs: a review Abstract: The transmission of infectious agents from domestic animals to humans is a matter of particular concern at present. Inoculation can enhance the defences of each individual animal but only in the short term. Certainly, it will be of immense benefit if biotechnology and genetic techniques are applied to farm animal breeding and selection programs to improve productivity, performance and health status as well as for the construction of sustainable animal production systems and promotion of animal welfare. In recent years, efforts to drive candidate genes like cytokines, haptoglobin, complement system, C-reactive protein, a 2-macroglobulin, retinol binding protein, transcortin, and etc. associated with immune traits have successfully been studied in human and different animal species. Here, we compared the molecular structure and evaluated the expression tendency of the haemolytic complement activity (HCA) of porcine candidate genes encoding the terminal complement components (TCC) C6-9. The results suggested that (1) high homology of complement genes among mammalian species may open new ways in cure/ treatment of disease; (2) Muong Khuong animals (Vietnamese potbelly pig) have a great genetic potential to improve the health status of pigs; and (3) HCA in the classical pathway can be developed further by different activation modes, with the potential improvement of animal health. Keywords: pigs, terminal complement genes, homology, genetic variation, and haemolytic complement activity Journal: Veterinární medicína Pages: 1-9 Volume: 58 Issue: 1 Year: 2013 DOI: 10.17221/6650-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6650-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201301-0001.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:1:id:6650-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Cech Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: J. Mala Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: E. Indrova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: M. Lopatarova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: R. Dolezel Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: H. Dluhosova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: L. Zilka Author-Workplace-Name: MEDIN, a.s. Nove Mesto na Morave, Czech Republic Title: The introduction of a double-channel system for the intrafollicular treatment of cattle Abstract: The aim of this study was to evaluate a new double-channel system for ultrasound-guided transvaginal intrafollicular treatment in cattle. The system is equipped with separate aspiration and treatment channels facilitating the aspiration of a small part of follicular fluid followed by the immediate injection of the same amount of treatment solution. In Experiment 1 an intracystic injection was performed successfully in all cows (6/6). In Experiment 2 saline was administered to dominant follicles with an overall success rate of 87.5% (14/16). This new double-channel system represents a reliable method for intrafollicular treatment preceded by an aspiration of the necessary amount of follicular fluid without an increase in intrafollicular pressure. Keywords: transvaginal aspiration, intrafollicular injection, intracystic injection, double-channel device, cow Journal: Veterinární medicína Pages: 10-15 Volume: 58 Issue: 1 Year: 2013 DOI: 10.17221/6651-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6651-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201301-0002.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:1:id:6651-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Gesek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: J. Szarek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: I. Otrocka-Domagala Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: I. Babinska Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: K. Pazdzior Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: M. Szweda Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Andrzejewska Author-Workplace-Name: Medical University, Bialystok, Poland Author-Name: B. Szynaka Author-Workplace-Name: Medical University, Bialystok, Poland Title: Morphological pattern of the livers of different lines of broiler chickens during rearing Abstract: The aim of this study was to analyse the microscopic and ultrastructural lesions of the livers of broiler chickens during fattening. Three genetic lines of broiler chickens (Ross 308, Cobb 500, Hubbard F15) were investigated. The liver samples were taken on the 3rd, 10th, 17th, 24th, 31st, and 38th day of life from six healthy broiler chickens from each commercial broiler flock. The dominant microscopic lesions were associated with prolonged hypoxia and bile ductules, including: fatty, vacuolar and parenchymatous degeneration, necrosis of epithelial cells of bile ductules, necrosis of hepatocytes around the proliferating bile ductules, lymphoid cell infiltration around the bile ductules and blood vessels, proliferation of the bile ductules, proliferation of the connective tissue around bile ductules and stimulation of the lymph nodules. Ultrastructural evaluation revealed abnormalities involving mitochondria and rough endoplasmic reticulum. The mitochondria underwent swelling, polymorphism, proliferation and damage. The rough endoplasmic reticulum underwent defragmentation and acinar transformation. The cytoplasm of most hepatocytes showed vacuoles of varying size or lipid droplets and the presence of cytoplasmic myelin-like structures. This study shows that the livers of broiler chickens are the most predisposed to the occurrence of lesions on the 17th, 31st and 38th days of life. Keywords: broiler chicken, Cobb, Ross, Hubbard, liver, pathomorphology Journal: Veterinární medicína Pages: 16-24 Volume: 58 Issue: 1 Year: 2013 DOI: 10.17221/6652-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6652-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201301-0003.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:1:id:6652-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N. Brkljaca Bottegaro Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: J. Kos Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: B. Pirkic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: O. Smolec Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Z. Grabarevic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M. Hohsteter Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: J. Selanec Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Z. Vrbanac Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Reduction of epidural fibrosis after laminectomy in rabbits by omental free graft Abstract: Epidural fibrosis is an extradural scar tissue formed after a laminectomy procedure. It is associated with persistent pain after spinal surgery and an increased risk of complications during revision surgery. The aim of this study was to determine the preventive effects of local application of an omental free graft in minimising spinal epidural fibrosis in a rabbit laminectomy model. Twenty two rabbits were randomly divided in two groups, a control group of seven and an experimental group of 15 animals. A dorsal laminectomy at levels L1 to L3 was performed on each rabbit of both groups. Prior to the laminectomy procedure, the animals from the experimental group were submitted to a laparotomy in order to obtain the free omental graft. The graft was then applied to the same animal at the dural deffect. All rabbits were euthanised six weeks after surgery and spine segments L1 to L3 were removed. Histological sections were evaluated for fibrosis intensity at the laminectomy level, the adhesion degree between dura mater and fibrous tissue and the presence of the foreign body reaction. A statistically significant correlation was established for the foreign body reaction presence and belonging to the group, which can be explained by the omental effects on inflammation reduction and healing promotion. The degree of adhesion between the dura mater and fibrous tissue and the intensity of the fibrous tissue at the laminectomy level were lower in the experimental group although the differences were not statistically significant. The use of free omental grafts is thus a promising technique in epidural fibrosis prevention. Keywords: spinal surgery, extradural fibrous tissue, omentum, rabbit model Journal: Veterinární medicína Pages: 25-31 Volume: 58 Issue: 1 Year: 2013 DOI: 10.17221/6653-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6653-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201301-0004.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:1:id:6653-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Dybus Author-Workplace-Name: West Pomeranian University of Technology, Szczecin, Poland Author-Name: W.S. Proskura Author-Workplace-Name: West Pomeranian University of Technology, Szczecin, Poland Author-Name: S. Sadkowski Author-Workplace-Name: West Pomeranian University of Technology, Szczecin, Poland Author-Name: E. Pawlina Author-Workplace-Name: Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Title: A single nucleotide polymorphism in exon 3 of the myostatin gene in different breeds of domestic pigeon (Columba livia var. domestica) 32 Abstract: Myostatin is considered to be one of the most powerful negative regulators of muscle growth. The lack of a functional myostatin or its mutation can result in uncommon musculature like "double-muscling" in Belgian Blue cattle. Recent studies on MSTN variability indicate its relationship with racing performance in dogs and racehorses. Considering the high homology of MSTN among the vertebrates, there are grounds to suppose that the same correlation will occur in pigeons. The aim of this study was to analyse MSTN variability in several pigeon breeds raised for different purposes The PCR-RFLP method was used for genotyping the C → T silent substitution in exon 3 of the MSTN gene. A total of 376 domestic pigeons (Columba livia var. domestica) were genotyped. The differences in genotype frequencies (P < 0.01) and allele frequencies (P < 0.01), between the studied groups were observed. Minor allele (MSTNT) frequency was the highest in the group of utility pigeons (0.291), which are characterised by abundant muscle mass and higher body mass-to-muscle mass ratio. Further studies should be performed in order to determine the impact of the SNP analysed in the present paper on the amount of functional myostatin in muscles. Keywords: myostatin, polymorphism, synonymous mutation, muscle growth Journal: Veterinární medicína Pages: 32-38 Volume: 58 Issue: 1 Year: 2013 DOI: 10.17221/6654-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6654-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201301-0005.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:1:id:6654-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Gorna Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland Author-Name: L. Adaszek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland Author-Name: K. Policht Author-Workplace-Name: Dogs and Cats Ambulatory, Przemysl, Poland Author-Name: M. Skrzypczak Author-Workplace-Name: School of Medicine, Skubiszewski University, Lublin, Poland Author-Name: S. Winiarczyk Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland Title: Detection of Anaplasma phagocytophilum in a cat Abstract: The aim of this study was to diagnose the etiological factor of disease in a 2.5 year old male cat with symptoms of loss of appetite, apathy and the presence of pale yellow mucous membranes. Haematological and biochemical analysis of the blood taken from the sick animal showed a normal white blood cell count (14.19 × 109/l), reduced erythrocyte (3.96 × 1012/l), haematocrit (0.2213), haemoglobin concentration (66 g/l) and thrombocyte count (52 × 109/l), increased activity of ALT (583 IU/l), AST (265 IU/l) and bilirubin concentration (205.2 µmol/l). A PCR test for Babesia/Theileria and Ehrlichia/Anaplasma spp. did not show the presence of the genetic material of protozoa; however, it revealed the presence of the 16S RNA gene of ricketsiae in the blood of the cat. The sequence of the fragment of this gene showed a 98.3% homology with Anaplasma phagocytophilum GU183908. Serological examination of the cat using the IFAT test revealed the presence in its serum of anti-Anaplasma phagocytophilum antibodies with a titre of 1 : 40. This paper presents the first report of feline anaplasmosis in Poland confirmed by molecular tests. Keywords: Anaplasma phagocytophilum, cat, PCR, tick-borne diseases Journal: Veterinární medicína Pages: 39-43 Volume: 58 Issue: 1 Year: 2013 DOI: 10.17221/6655-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6655-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201301-0006.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:1:id:6655-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Guadarrama-Olhovich Author-Workplace-Name: Faculty of Veterinary Medicine and Husbandry, National Autonomous University of Mexico, Mexico City, Mexico Author-Name: L.E. Garcia Ortuno Author-Workplace-Name: Faculty of Veterinary Medicine and Husbandry, National Autonomous University of Mexico, Mexico City, Mexico Author-Name: J.A. Ruiz Remolina Author-Workplace-Name: Faculty of Veterinary Medicine and Husbandry, National Autonomous University of Mexico, Mexico City, Mexico Author-Name: C. Lopez Buitrago Author-Workplace-Name: Faculty of Veterinary Medicine and Husbandry, National Autonomous University of Mexico, Mexico City, Mexico Author-Name: J. Ramirez Lezama Author-Workplace-Name: Faculty of Veterinary Medicine and Husbandry, National Autonomous University of Mexico, Mexico City, Mexico Author-Name: J. Bouda Author-Workplace-Name: Faculty of Veterinary Medicine and Husbandry, National Autonomous University of Mexico, Mexico City, Mexico Title: Acute pancreatitis, azotaemia, cholestasis and haemolytic anaemia in a dog: a case report Abstract: We here report acute pancreatitis with multiorganic complications in a female Cocker Spaniel. The most important alterations in clinical pathology were renal azotaemia, hyperbilirubinaemia with a prevalence of conjugated bilirrubin and increased alkaline phosphatase by cholestasis; hyperamylasaemia due to pancreatitis; dehydration, hyponatraemia, hypochloraemia and hypokalaemia related to vomiting; metabolic acidosis and respiratory alkalosis corresponding to mixed acid-base disorder; markedly regenerative anaemia with spherocytes and agglutination due to immune-mediated haemolytic anaemia and intravascular haemolysis; leukocytosis with a left shift. Proteinuria, glucosuria, bilirubinuria and haemoglobinuria were detected in the urine. Severe suppurative pancreatitis with peripancreatic necrosis and suppurative esteatitis, tubulorrhectic nephrosis, severe hepatitis and intrahepatic cholestasis corresponded with alterations described by clinical pathology. In order to diagnose acute pancreatitis in dogs with multiple complications, it is very important to integrate the results of clinical pathology with the anamnesis and physical examination of the animal. It is especially important to note that the serum activity of amylase correlates with time after the pancreatic attack. Keywords: exocrine pancreas, multiorganic complications, clinical pathology, necropsy, dog Journal: Veterinární medicína Pages: 44-49 Volume: 58 Issue: 1 Year: 2013 DOI: 10.17221/6657-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6657-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201301-0007.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:1:id:6657-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.R. Alam Author-Workplace-Name: Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh Author-Name: W.J. Gordon Author-Workplace-Name: Wisconsin Veterinary Referral Center, Waukesha, Wisconsin, USA Author-Name: S.Y. Heo Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea Author-Name: K.C. Lee Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea Author-Name: N.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea Author-Name: M.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea Author-Name: H.B. Lee Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea Title: Augmentation of ruptured tendon using fresh frozen Achilles tendon allograft in two dogs: a case report Abstract: This article describes two cases of augmentation of ruptured tendon with fresh frozen Achilles tendon allograft (FFATA) in dogs. Case 1 was a two-year-old crossbreed dog (29 kg) that presented with an open wound on the right forelimb and with complete rupture of the flexor carpi ulnaris and superficial digital flexor tendons. Case 2 was a four-year-old crossbreed dog (4 kg) with partial ruptures of the patellar tendon and detachment of the tibial tuberosity in the right hind limb. In both cases, the ends of the ruptured tendon were sutured and apposed after debridement. To minimize suture failure, FFATA (cut to sufficient size) was placed across the primary suture with tension and sutured to the host tendon. In addition, Case 2 received a Krackow suture through a transverse bone tunnel made in the tibia to fix the patellar tendon along with the tibial tuberosity in situ. The surgical areas healed without any evidence of exaggerated inflammatory response or clinical signs consistent with rejection of the allograft. Both the dogs had normal ambulation and weight bearing on the affected limb 12 weeks postoperatively. No postoperative complications were observed during a one-year follow up period except for slight contracture of the carpus and digits of the affected limb in Case 1. Thus, ruptured tendons can be successfully repaired using suture and augmentation with FFCTA. Augmentation with FFATA may provide additional stability, which counters tension on the primary repair and reduces the chance of gap formation or suture failure in case of reconstruction of the damaged tendon in dogs. Keywords: tendon rupture, Achilles tendon allograft, augmentation, dog Journal: Veterinární medicína Pages: 50-55 Volume: 58 Issue: 1 Year: 2013 DOI: 10.17221/6656-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6656-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201301-0008.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:1:id:6656-VETMED