Template-Type: ReDIF-Article 1.0 Author-Name: B. Kempisty Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Author-Workplace-Name: Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland Author-Name: D. Bukowska Author-Workplace-Name: Department of Veterinary Science, Poznan University of Life Sciences, Poznan, Poland Author-Name: M. Wozna Author-Workplace-Name: Department of Veterinary Science, Poznan University of Life Sciences, Poznan, Poland Author-Name: H. Piotrowska Author-Workplace-Name: Department of Toxicology, Poznan University of Medical Sciences, Poznan, Poland Author-Name: M. Jackowska Author-Workplace-Name: Department of Veterinary Science, Poznan University of Life Sciences, Poznan, Poland Author-Name: A. Zuraw Author-Workplace-Name: Department of Veterinary Science, Poznan University of Life Sciences, Poznan, Poland Author-Name: S. Ciesiolka Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Author-Name: P. Antosik Author-Workplace-Name: Department of Veterinary Science, Poznan University of Life Sciences, Poznan, Poland Author-Name: H. Maryniak Author-Workplace-Name: Institute of Zoology, Department of Animal Anatomy, Poznan University of Life Sciences, Poznan, Poland Author-Name: E. Ociepa Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Author-Name: Sz. Porowski Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Author-Name: K.P. Brussow Author-Workplace-Name: Department of Reproductive Biology, Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany Author-Name: J.M. Jaskowski Author-Workplace-Name: Department of Veterinary Science, Poznan University of Life Sciences, Poznan, Poland Author-Name: M. Nowicki Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Title: Endometritis and pyometra in bitches: a review Abstract: Endometritis-pyometra is the most frequent and complex pathology in domestic bitches. This process involves several immunological changes as well as molecular mechanisms responsible for inflammation in the female uterus. The various clinical stages of pyometra are associated with various symptoms. In this review, several aspects are described, including physiological and pathological mechanisms as well as molecular changes which take place during induction of endometritis-pyometra. The authors also highlight the important role of growth factors and their receptors in this process. It is well known that pyometra is a compound process which mainly involves immunological changes during inflammation. However, this review presents a new overview of this process, which includes changes at the molecular level, e.g., the altered expression of genes crucial for the development of this disease. Although pyometra is the most frequent disease of the reproductive tract in bitches, the molecular basis of this process is still not entirely understood. Keywords: uterine diseases, cystic endometrial hyperplasia-pyometra complex, endometritis-pyometra complex Journal: Veterinární medicína Pages: 289-297 Volume: 58 Issue: 6 Year: 2013 DOI: 10.17221/6864-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6864-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201306-0001.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:6:id:6864-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Faldynova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: P. Videnska Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: H. Havlickova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: F. Sisak Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: H. Juricova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: V. Babak Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: L. Steinhauser Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: I. Rychlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Prevalence of antibiotic resistance genes in faecal samples from cattle, pigs and poultry Abstract: Antibiotic resistant bacteria can be easily isolated from the faeces of cattle, pigs or poultry. However, whether the production of different farm animals is associated with a higher or lower prevalence of antibiotic resistance is not clear. In this study we therefore used real time PCR for the quantification of antibiotic gene prevalence in the DNA purified from the faeces of farm animals. First we showed that experimental streptomycin therapy of 12-week-old chickens and 46-week-old hens significantly increased the relative prevalence of strA and sul2 genes though this did not necessarily indicate an absolute increase of strA-encoding bacteria. Next we quantified antibiotic gene prevalence in the DNA purified from the faeces of cattle, pigs and laying hens. The lowest prevalence of strA, aadA, sul1, sul2, tet(A), tet(B), tet(G) and cat genes was recorded in the intestinal contents of laying hens. In cattle and pig faecal samples, an intermediate prevalence of antibiotic resistance genes was observed with strA and sul2 dominating by two logs over the remaining six tested genes. The differences in strA and sul2 prevalence between cattle and pig microbiota were not significant whilst the prevalence of strA and sul2 in laying hen microbiota was significantly lower than in the other two species. Cattle and pig production systems may therefore represent a more important reservoir of antibiotic resistant bacteria than laying hens. Keywords: real time PCR, antibiotic resistance, farm animals, chicken, pig, cattle Journal: Veterinární medicína Pages: 298-304 Volume: 58 Issue: 6 Year: 2013 DOI: 10.17221/6865-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6865-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201306-0002.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:6:id:6865-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Roldan-Santiago Author-Workplace-Name: Postgraduate Division of Animal Production Sciences and Health, Universidad Nacional Autonoma de Mexico (UNAM), Mexico Author-Name: D. Mota-Rojas Author-Workplace-Name: Department of Animal Production and Agriculture, Universidad Autonoma Metropolitana, Mexico Author-Name: I. Guerrero-Legarreta Author-Workplace-Name: Department of Biotechnology, Universidad Autonoma Metropolitana, Mexico Author-Name: P. Mora-Medina Author-Workplace-Name: Department of Livestock Sciences, Universidad Nacional Autonoma de Mexico (UNAM), Mexico Author-Name: F. Borderas-Tordesillas Author-Workplace-Name: Department of Animal Production and Agriculture, Universidad Autonoma Metropolitana, Mexico Author-Name: A.D. Alarcon-Rojo Author-Workplace-Name: Department of Technology and Animal Products, Universidad Autonoma de Chihuahua (UACH), Mexico Author-Name: S. Flores-Peinado Author-Workplace-Name: Department of Livestock Sciences, Universidad Nacional Autonoma de Mexico (UNAM), Mexico Author-Name: H. Orozco-Gregorio Author-Workplace-Name: Department of Animal Production and Agriculture, Universidad Autonoma Metropolitana, Mexico Author-Name: R. Martinez-Rodriguez Author-Workplace-Name: Department of Animal Production: Swine and CEIEPP, Universidad Nacional Autonoma de Mexico (UNAM), Mexico Author-Name: M.E. Trujillo-Ortega Author-Workplace-Name: Department of Animal Production: Swine and CEIEPP, Universidad Nacional Autonoma de Mexico (UNAM), Mexico Title: Animal welfare of barrows with different antemortem lairage times without food Abstract: This study evaluated the effect of five different periods of antemortem lairage without food on the energy metabolism, gas exchange, mineral and blood acid-base balances of 1174 hybrid barrows, which were divided into six treatment groups according to the lairage period: 130 barrows were considered for the evaluation of the baseline levels (GB); 214 had 0 h of lairage (R0); 228 had 4 h of lairage (R4); 204 had 8 h of lairage (R8); 192 had 12 h of lairage (R12); and 206 had 24 h (R24). In all groups, increasing lairage periods triggered a significant reduction (P < 0.05) in pH, accumulation of lactic acid and percentage of hematocrit. These findings led to the conclusion that antemortem lairage periods longer than 4 h cause hyperglycaemia, hypercalcaemia, hyperlactataemia, hyperkalaemia, hyponatraemia, acidosis, and more severe dehydration in barrows. Keywords: rest, hemodynamic response, stress, pig, lactate Journal: Veterinární medicína Pages: 305-311 Volume: 58 Issue: 6 Year: 2013 DOI: 10.17221/6866-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6866-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201306-0003.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:6:id:6866-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Rauser Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: P. Janalik Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: M. Markova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: T. Fichtel Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Title: Early analgesia after periodontal treatment in dogs: a comparison of three analgesic protocols Abstract: The analgesic effects of carprofen, morphine and bupivacaine on early oral pain after periodontal treatment in dogs have been poorly investigated. Forty-five client-owned dogs (8.5 ± 6.4 kg and 7.8 ± 3.2 years) scheduled for periodontal treatment were allocated to carprofen, morphine and bupivacaine groups (n = 15 each). The study was designed as a prospective, randomised, double "blinded" clinical study. Carprofen (CAR, 4 mg/kg, subcutaneously) or morphine (MOR, 0.3 mg/kg, intramuscularly) was given thirty minutes before the dogs were placed under anesthesia. Bilateral maxillary and mandibular nerve blocks were performed with bupivacaine (BUP, 1 mg/kg), after the induction of anesthesia. Dogs were anaesthetised with medetomidine-propofol-isoflurane and reversal was carried out using atipamezole. Periodontal painful sub-gingival scaling was performed in all dogs. Periodontal treatment lasted for up to one hour. A modified University of Melbourne Pain Score (UMPS, 0-28 points), Visual Analog pain Scale (VAS, 0-100 mm), plasma glucose (Glu) and serum cortisol (Cor) levels were assessed before administration of analgesics (MOR-0, CAR-0, BUP-0) and two hours thereafter, that is thirty minutes after atipamezole administration (MOR-2, CAR-2, BUP-2). Analgesia rescue with tramadol (2 mg/kg intramuscularly) was provided for animals with modified UMPS over 14 or VAS over 50 points. Differences in Glu and Cor values were analysed with analysis of variance (ANOVA) for repeated measures, in UMPS and VAS over time for each group with the Friedman test and pre- and postoperatively using the Mann-Whitney U-test. Differences were considered significant at P < 0.05. Analgesia rescue was provided to one patient of the CAR group and one patient of the MOR group. No differences in UMPS values between groups were detected. A significant increase in VAS values after treatment was detected in all groups. Plasma glucose levels significantly increased in MOR-2 compared to MOR-0 and CAR-2. Serum cortisol levels significantly increased in MOR-2 compared to MOR-0, CAR-2 and BUP-2. The results of this study indicate that bupivacaine nerve blocks could be superior to carprofen, which in turn could be superior to morphine, for early analgesia (up to two hours) following sub-gingival scaling for periodontal treatment in dogs. Keywords: oral pain, carprofen, morphine, bupivacaine, teeth Journal: Veterinární medicína Pages: 312-317 Volume: 58 Issue: 6 Year: 2013 DOI: 10.17221/6867-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6867-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201306-0004.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:6:id:6867-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Yilmaz Author-Workplace-Name: Veterinary Faculty, Firat University, Elazig, Turkey Author-Name: A. Aydin Author-Workplace-Name: School of Medicine, Adiyaman University, Adiyaman, Turkey Author-Name: G. Dinc Author-Workplace-Name: Veterinary Faculty, Firat University, Elazig, Turkey Author-Name: B. Toprak Author-Workplace-Name: Etlik Central Veterinary Control and Research Institute, Ankara, Turkey Author-Name: M. Karan Author-Workplace-Name: Veterinary Faculty, Firat University, Elazig, Turkey Title: Investigations on the postnatal development of the foliate papillae using light and scanning electron microscopy in the porcupine (Hystrix cristata) Abstract: In this study SEM and light microscopy were used to investigate the structure of the foliate papillae in the porcupine. The foliate papillae consisted of about 10 or 11 clefts. The length of the foliate papillae averaged 2.79 mm and its width averaged 863 µm. Taste buds were located intraepithelial in the basal half of the papilla grooves (sulcus papillae). Every wall on each fold harboured from five to nine taste buds. There were two different cell types of taste buds: one stained light (epitheliocytus sensorius gustatorius), and the other dark (epitheliocytus sustentans). The length and width of the taste buds averaged 190.5 µm and 86 µm, respectively. The ratio of the length to the width of taste buds was 2.21. The average depth of the papilla groves was 1763 µm and its epithelial thickness was 235.5 µm. In scanning electron microscopy, the thickness of the epithelial cell borders was apparent at higher magnifications and there micro-ridges and micro-pits were apparent on the surfaces of these cells. Keywords: porcupine, Hystrix cristata, foliate papillae, scanning electron microscope (SEM) Journal: Veterinární medicína Pages: 318-321 Volume: 58 Issue: 6 Year: 2013 DOI: 10.17221/6868-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6868-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201306-0005.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:6:id:6868-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Zert Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: S. Krisova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: K. Zuffova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Pastern joint arthrodesis using two paraaxial and one axial crossed lag screws: a case report Abstract: Arthrodesis was achieved in two foals through the placement of three 4.5 mm cortical lag screws. Two screws were placed in a standard transarticular fashion from the dorsal aspect of P1 (glide hole) to the palmar aspect of P2 (thread hole). A third transarticular lag screw was placed from the dorsal aspect of P2 (glide hole) to the palmar aspect of P1 (thread hole). Both foals were comfortable and considered suitable for light work. Crossed transarticular lag screw fixation of P1 and P2 in cases of PIPJ arthrodesis in foals is a simple and useful method offering good stability for fusion and future athletic soundness. Keywords: foal, joint fusion, immaturity, osteochondrosis, osteosynthesis Journal: Veterinární medicína Pages: 322-326 Volume: 58 Issue: 6 Year: 2013 DOI: 10.17221/6869-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6869-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201306-0006.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:6:id:6869-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Lew-Kojrys Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: Z. Adamiak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Pomianowski Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: W. Maksymowicz Author-Workplace-Name: Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland Author-Name: M. Barczewska Author-Workplace-Name: Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Majchrowski Author-Workplace-Name: Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland Title: Percutaneous laser disc decompression in the treatment of detrusor-urethral dyssynergia in a female German Shepherd: a case report Abstract: A 7-year-old female, German Shepherd with urinary and faecal voiding difficulties was admitted to the clinic. The patient was subjected to neurological, ultrasonographic and radiological examinations as well as blood and urine tests. Based on clinical observations and test results, the dog was diagnosed with detrusor-urethral dyssynergia. The prescribed pharmacological treatment consisted of alpha adrenergic receptor antagonists and skeletal muscle relaxants. The patient was catheterised daily, and Foley's catheter was temporarily inserted. Pharmacological treatment was not effective, however, and the patient was subjected to an MRI examination of the lumbosacral spine which revealed L6-L7 dyscopathy. The dog was then subjected to percutaneous laser disc decompression. Pharmacological treatment was continued, and the catheter was left in place after surgery. Active urination was restored four days after surgery despite the presence of the catheter. The catheter was subsequently removed, and pharmacological treatment was gradually discontinued. The symptoms of dyssynergia subsided completely. Keywords: detrusor-urethral dyssynergia, dog, PLDD, magnetic resonance Journal: Veterinární medicína Pages: 327-330 Volume: 58 Issue: 6 Year: 2013 DOI: 10.17221/6870-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6870-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201306-0007.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:6:id:6870-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Jelinek Author-Workplace-Name: Veterinary Histopathological Laboratory, Prague, Czech Republic Title: Gliomatosis of the spinal cord in a cat: a case report Abstract: Gliomatosis cerebri situated in the thoracolumbar and lumbosacral segments of the spinal cord was diagnosed in a Persian 10.5-year old tom cat. Clinical manifestation consisted of rump ataxy, weakened posture in the hind limbs, paraparesis, weakened spinal reflexes, no spinal hyperaesthesia or pain, and hypotonia of the urinary bladder. Magnetic resonance imaging revealed a diffuse hyperintense signal in the thoracolumbar junction and in lumbosacral segments (L6-S1) of the spinal cord. Normal size, form and colour of the spinal cord were apparent at autopsy; however, changes in grey matter shape were recorded on some transversal sections of the spinal cord. Dense, diffuse infiltration of the grey matter and surrounding white matter by glia-like neoplastic cells was histologically demonstrated. Immunohistochemical examination did not enable specification of the origin of the neoplastic cells but their neuroectodermal histogenesis can be assumed. On the basis of MRI, histological and immunohistochemical examinations gliomatosis of the spinal cord was diagnosed. Keywords: feline, medulla, neoplasia, clinic, pathology Journal: Veterinární medicína Pages: 331-337 Volume: 58 Issue: 6 Year: 2013 DOI: 10.17221/6871-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6871-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201306-0008.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:6:id:6871-VETMED