Template-Type: ReDIF-Article 1.0 Author-Name: S. Samiullah Author-Workplace-Name: School of Environmental and Rural Science, University of New England, Armidale, Australia Title: Contagious caprine pleuropneumonia and its current picture in Pakistan: a review Abstract: Contagious caprine pleuropneumonia (CCPP) is caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp) which belongs to the Mycoplasma mycoides cluster, a group of five closely related Mycoplasmas, pathogenic to ruminants. The true lesions of CCPP are restricted to the alveolar tissues of infected goats, which distinguish it from other respiratory diseases of small ruminants caused by members of the Mycoplasma mycoides cluster. The typical signs of CCPP are an accumulation of pleural fluid, unilateral hepatisation, adhesions, pleurisy and pleuropneumonia which clearly differentiate it from "MAKePS" syndrome caused by Mycoplasma mycoides subsp. capri (Mmc). The available literature on CCPP shows that so far in Pakistan, the true causative agent (Mccp) of this disease has only been isolated in the Pashin District of Balochistan and that the disease is more frequently confused with other respiratory diseases of goat caused by the Mycoplasma mycoides cluster. The lack of suitable techniques and extensive knowledge in the field is a big limitation for the isolation and characterisation of Mccp from prevailing CCPP-like cases in the goat population of Pakistan. Keywords: goat, Mycoplasma mycoides cluster, Mycoplasma capricolum subsp. capripneumoniae, diagnostic tests, respiratory diseases Journal: Veterinární medicína Pages: 389-398 Volume: 58 Issue: 8 Year: 2013 DOI: 10.17221/6977-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6977-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201308-0001.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:8:id:6977-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.M. Nazmul Alam Author-Workplace-Name: Department of Environment and Agriculture, Curtin University, Perth, Australia Title: Bangladesh in the Rapid Alert System for Food and Feed notifications in the period 2000-2012: a review Abstract: Information provided in the Rapid Alert System for Food and Feed (RASFF) portal database of the European Commission on "crustaceans and products thereof" for Bangladesh during 2000-2012 was investigated to analyse the trends of occurrences. A total of 159 (10.56%) notifications (alert - 40; border rejection - 47; information - 72) were recorded for Bangladesh against a worldwide 1505 notifications. During the period 120 (20.27%) notifications were identified for residue of veterinary medicinal products against 592 notifications recorded in the portal. Forty-eight consignments were re-despatched to Bangladesh while 11 consignments were destroyed at the European border as a consequence of the notifications by the European countries. Keywords: crustaceans, RASFF, rejection, notification, veterinary medicinal product Journal: Veterinární medicína Pages: 399-404 Volume: 58 Issue: 8 Year: 2013 DOI: 10.17221/6978-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6978-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201308-0002.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:8:id:6978-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J.M. Milani Author-Workplace-Name: Department of Food Science and Technology, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Title: Ecological conditions affecting mycotoxin production in cereals: a review Abstract: Spoilage fungi are ubiquitous contaminants of cereals, pre- and post-harvest. These fungi can produce a wide range of secondary metabolites under ecological conditions which are conducive for growth. However, some of these secondary metabolites are toxic and have a significant impact if they enter the production and animal food chains. Prevention of mycotoxin contamination of feed and food raw materials is now considered more important than subsequent cure. The key ecological determinants pre- and post-harvest are water availability and temperature (climate). Accurate information is therefore needed on the impact of an association between these key factors, and it is necessary to understand which are marginal and which critical for germination and toxin production. There have only been a few studies where attempts have been made to integrate the available information on these factors in relation to different raw materials for feed and food processing, especially cereals. This review will examine the available information on the main climatic factors, i.e., water availability and temperature affecting mycotoxin production such as, aflatoxins, ochratoxins, fumonisins, zearalenone, deoxynivalenol and citrinin. This information is crucial for accurately focusing and monitoring key critical control points in the feed and food chain to optimise prevention strategies. Keywords: climate, mycotoxins, cereals, ecology, post-harvest, fungi Journal: Veterinární medicína Pages: 405-411 Volume: 58 Issue: 8 Year: 2013 DOI: 10.17221/6979-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6979-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201308-0003.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:8:id:6979-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K.-P. Brüssow Author-Workplace-Name: Leibniz Institute for Farm Animal Biology (FBN), Institute of Reproductive Biology, Dummerstorf, Germany Author-Name: A. Vernunft Author-Workplace-Name: Leibniz Institute for Farm Animal Biology (FBN), Institute of Reproductive Biology, Dummerstorf, Germany Author-Name: B. Kempisty Author-Workplace-Name: Department of Histology and Embryology, and Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland Author-Name: J. Ratky Author-Workplace-Name: Research Institute for Animal Breeding and Nutrition (ATK), Herceghalom, Hungary Title: Single fixed-time laparoscopic intrauterine insemination as a tool to obtain low-diversity porcine embryos Abstract: Double fixed-time insemination after ovulation induction is commonly used in pigs to obtain in vivo produced embryos at defined stages of development for downstream biotechnological applications. However, variations in the time of ovulation and fertilisation of the ovulated oocytes by spermatozoa, mainly in one of the inseminations, can cause diversities in embryo development. The aim of the present study was to reduce embryo diversity and to achieve a 'uniform outcome' of porcine embryo stages using single laparoscopic fixed-time insemination (LIUI). Altogether, 48 puberal German Landrace gilts were included in the study. Estrus of gilts was synchronized by 15-day long altrenogest (Regumate®) feeding and follicle development was stimulated with 850 IU eCG 24 h after the final altrenogest application. Ovulation was induced with 500 IU hCG 80 h after eCG. LIUI was performed 31 h after hCG treatment. Gilts under general anaesthesia were fixed in a dorsal position, a pneumoperitoneum was produced and three trocar cannulas were inserted into the abdomen for optics and instruments. Each uterine horn was carefully punctured 10-15 cm caudal from the utero-tubal junction with a 2.5 mm trocar. A 2.2 mm catheter was inserted about 3 cm into the uterine lumen and 20 ml of extended fresh boar semen (32.2 × 106 sperm cells/ml) were injected. Embryos were surgically flushed from the genital tract two (Day 2) and three (Day 3) days after insemination. Altogether, 778 oocytes/embryos were recovered (recovery rate 68 ± 17%); 45 of 48 gilts (93.8%) revealed fertilisation and 76.1% of the recovered embryos (n = 592) were at the 2- and 4-cell stage. On Day 2 (n = 22 gilts), a higher percentage of gilts (72.7%, P < 0.05) displayed only 2-cell embryos compared with gilts which had 2- and 4-cell (22.7%), or only 4-cell embryos (4.6%). On Day 3 (n = 23 gilts), the proportion of gilts with 2-cell, 2- and 4-cell, and only 4-cell embryos shifted to 4.3%, 0% and 95.7%, respectively (P < 0.05). The results of the present study demonstrate high rates of fertilisation and homogenously developed embryos after single fixed-time laparoscopic intrauterine insemination in gilts. Additionally, these results were achieved by inseminating a 60% lower number of sperm cells per insemination dose compared to usual doses used for transcervical insemination. In conclusion, LIUI can be recommended for the in vivo production of embryos in a homogeneous developmental stage, and also as an alternative method for low-dose insemination. Keywords: laparoscopic insemination, embryo diversity, pig Journal: Veterinární medicína Pages: 412-416 Volume: 58 Issue: 8 Year: 2013 DOI: 10.17221/6980-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6980-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201308-0004.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:8:id:6980-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Aydogan Author-Workplace-Name: Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, Turkey Author-Name: M. Haligur Author-Workplace-Name: Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, Turkey Author-Name: O. Ozmen Author-Workplace-Name: Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, Turkey Title: The expression of caspase-3, caspase-7, caspase-9 and cytokeratin AE1/AE3 in goats with enzootic nasal adenocarcinoma: an immunohistochemical study Abstract: The aim of this study was to examine the expression of caspase-3, caspase-7, caspase-9 and cytokeratin AE-1/AE-3 using the avidin-biotin complex (ABC) immunoperoxidase technique in 20 goats with enzootic nasal adenocarcinoma (ENA). Clinically, dyspnoea and nasal discharge were observed in all cases. Macroscopically, polypoid and sessile masses were seen in the ethmoidal area. At the histopathological examination, tubular, papillary and mixed patterns of ENA were diagnosed. Immunohistochemically, strong positive reactions were generally seen for caspase-3, while strong to moderate and slight reactions were observed for caspase-7 and caspase-9 in the cytoplasm of the tumour cells. Positive reactions for cytokeratin AE-1/AE-3 were only seen in epithelial cells. In addition, the causative agent of ENA, retrovirus, was detected immunohistochemically in tumour cells. Keywords: enzootic nasal adenocarcinoma, caspase-3, caspase-7, caspase-9, cytokeratin A Journal: Veterinární medicína Pages: 417-421 Volume: 58 Issue: 8 Year: 2013 DOI: 10.17221/6981-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6981-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201308-0005.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:8:id:6981-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Tlak Gajger Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: S. Nejedli Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Z. Kozaric Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: The effect of Nozevit on leucine aminopeptidase and esterase activity in the midgut of honey bees (Apis mellifera) Abstract: The histochemical activity of aminopeptidase and non-specific esterase, both important enzymes of intermediate metabolism in the mid gut of honeybees (Apis mellifera), was investigated. Nosema disease control poses a major challenge, and thus, the treatment of this serious parasitic disease using natural phyto-pharmacological preparations could be of great besnefit. Additionally, the effects of residues and their by-products in honey and wax represent an environmental concern and are another reason for reducing the use of conventional chemical control methods in beekeeping. Nozevit is a naturally prepared water solution of plant polyphenols, and is distributed throughout the market as "the partner for Nosema disease repression". Samples of the midgut tissue were used for the description of enzymatic activity of infected honey bees before treatment and 1, 2, 3, 5, 8 and 12 days after a one-time treatment with Nozevit. The possible effects of Nozevit treatment on the activity of the investigated enzymes and digestion are discussed. Keywords: honey bee (Apis mellifera), Nosema disease, Nozevit, LAP, esterase Journal: Veterinární medicína Pages: 422-429 Volume: 58 Issue: 8 Year: 2013 DOI: 10.17221/6982-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6982-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201308-0006.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:8:id:6982-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Szweda Author-Workplace-Name: University of Warmia and Mazury, Olsztyn, Poland Author-Name: J. Szarek Author-Workplace-Name: University of Warmia and Mazury, Olsztyn, Poland Author-Name: K. Dublan Author-Workplace-Name: University of Warmia and Mazury, Olsztyn, Poland Author-Name: M. Gesek Author-Workplace-Name: University of Warmia and Mazury, Olsztyn, Poland Author-Name: T. Mecik-Kronenberg Author-Workplace-Name: Medical University of Silesia, Zabrze, Poland Title: Effect of selected non-steroidal anti-inflammatory drugs on the pathomorphology of the mucous membrane of the canine colon Abstract: Non-steroidal anti-inflammatory drugs (NSAIDs) have been commonly used for the management of chronic pain caused by inflammatory joint disease in dogs. Although effective at relieving pain and inflammation, NSAIDs are associated with a significant risk of serious gastrointestinal side effects. The present study was therefore designed to investigate the effects of carprofen as a poorly-selective COX (cyclooxygenase) inhibitor and robenacoxib as a selective COX-2 inhibitor on the colon mucosa. A biopsy of the gastrointestinal tract was performed before treatment and on the last day of treatment with orally-administered carprofen (Group I), robenacoxib (Group II) and empty gelatine capsule (Group III) for twenty-one days in a randomised study. The most evident microscopic lesions in the colonic mucosa in young beagles were caused by a 21-day treatment with robenacoxib. The infiltration with inflammatory cells in the lamina propria of the colonic mucosa was the most commonly-found histopathological lesion. Keywords: carprofen, robenacoxib, cyclooxygenase, beagle, gastrointestinal tract, inflammatory cells Journal: Veterinární medicína Pages: 430-436 Volume: 58 Issue: 8 Year: 2013 DOI: 10.17221/6983-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6983-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201308-0007.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:8:id:6983-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J.K. Park Author-Workplace-Name: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: A.Y. Kim Author-Workplace-Name: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea Author-Name: E.M. Lee Author-Workplace-Name: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea Author-Name: E.J. Lee Author-Workplace-Name: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea Author-Name: D.M. Kwak Author-Workplace-Name: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea Author-Name: I.H. Hong Author-Workplace-Name: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: J.M. Cullen Author-Workplace-Name: College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA Author-Name: K.S. Jeong Author-Workplace-Name: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea Title: Granulomatous metritis caused by suspected Dirofilaria immitis in a dog: a case report Abstract: Here we describe a unique uterine mass in a dog with granulomatous lesions caused by filarial larvae from the family Onchocercidae. An 8-year-old female Maltese was presented to a local animal hospital with anorexia, depression, and vaginal discharge. A markedly distended uterus was observed on lateral abdominal radiographs, leading to a clinical diagnosis of pyometra or uterine mass of an unknown origin. During surgery, the left uterine horn contained a 5 cm diameter mass adhered to adjacent soft tissue. On gross inspection, the mass contained numerous white nematodes. Microscopically, this mass was characterised by a granulomatous inflammation of the myometrium and endometrium. Because all of the nematodes were dead, definitive species identification was not possible. However, based on the histologic appearance, these nematodes were tentatively identified as Dirofilaria immitis larvae. Keywords: dog, filarid, granuloma, larva, nematode, uterus Journal: Veterinární medicína Pages: 437-441 Volume: 58 Issue: 8 Year: 2013 DOI: 10.17221/6984-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6984-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201308-0008.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:8:id:6984-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Jelinek Author-Workplace-Name: Veterinary Histopathological Laboratory, Prague, Czech Republic Author-Name: J. Felsberg Author-Workplace-Name: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic Author-Name: M. Mestan Author-Workplace-Name: Veterinary Surgery, Prague, Czech Republic Title: Familial incidence of mammary gland tumours in the Djungarian hamster (Phodopus sungaros): a case report Abstract: Tumours of the mammary gland were diagnosed in one female Djungarian hamster (Phodopus sungaros) and in two of her daughters from two litters. Altogether, five tumours were diagnosed. Three of them were adenocarcinomas, one was adenoma with disseminated foci of adenocarcinoma and one was diagnosed as an atypical fibrosarcoma derived from cutaneous ganglion cell-like cells. This was a recurrent tumour in the proximity of the previously extirpated adenocarcinoma. Using specific PCR primers for conserved regions of the mouse mammary tumour virus (MMTV) no endogenous provirus DNA could be detected in DNA samples isolated from formalin-fixed paraffin embedded tissue sections. Keywords: hamsters, neoplasias, atypical fibrosarcoma, mammary gland, clinic, pathology, molecular biology Journal: Veterinární medicína Pages: 442-448 Volume: 58 Issue: 8 Year: 2013 DOI: 10.17221/6985-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6985-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201308-0009.txt Handle: RePEc:caa:jnlvet:v:58:y:2013:i:8:id:6985-VETMED