Template-Type: ReDIF-Article 1.0 Author-Name: M.Z. Felsmann Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: J. Szarek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: M. Felsmann Author-Workplace-Name: Faculty of Health Science, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Torun, Poland Author-Name: E. Strzyzewska Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Title: Protection of animals used in experiments in Polish law - history, present day and perspective: a review Abstract: The aim of EU legislators with respect to experiments on animals was to reduce to an absolute minimum both the number of animals used in experiments and their suffering. This study presents, from a historical point of view, expectations related to the coming into force on 1 January 2014 of Directive 2010/63/EU adopted on 22 September 2010. At the same time, it points out that, in some situations, carrying out experiments on animals in practice is different than in theory. The study calls attention to the issue of mere literal conformity of the Polish law with EU directives, illustrating it with examples and takes a critical view of the omission of veterinarians (Veterinary Inspection) from the supervision of experiments. Keywords: laboratory animals, experiments on animals, animal rights, animal welfare Journal: Veterinární medicína Pages: 117-123 Volume: 59 Issue: 3 Year: 2014 DOI: 10.17221/7378-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7378-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201403-0001.txt Handle: RePEc:caa:jnlvet:v:59:y:2014:i:3:id:7378-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Holak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland Author-Name: M. Jalynski Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland Author-Name: Z. Adamiak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland Author-Name: Z. Lekston Author-Workplace-Name: Faculty of Computer Science and Materials Science, University of Silesia in Katowice, Chorzow, Poland Author-Name: H. Morawiec Author-Workplace-Name: Faculty of Computer Science and Materials Science, University of Silesia in Katowice, Chorzow, Poland Author-Name: I. Otrocka-Domagala Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland Author-Name: P. Przyborowska Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland Author-Name: K. Pazdzior Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland Title: The use of shape memory NiTi alloy clips in small bowel anastomosis in pigs Abstract: The usefulness of compression anastomotic clips (CAC) for small bowel anastomosis in animals was evaluated. Implants made of nickel-titanium alloy (NiTi) in the form of elliptical double-coil springs with one-way and two-way shape memory were applied. The studied animals were subjected to jejunal resection and side-to-side bowel anastomosis with the use of CACs. The experiment was conducted on 10 pigs divided into three groups. The speed and ease of the surgical procedure, implant excretion time and leak tightness were evaluated by macroscopic observations and X-ray examinations. Tissue samples from the anastomotic site were subjected to histopathological analysis. The applied method was characterised by tight connections between fused tissue, the absence of complications and ease of use. The results of the study indicate that shape memory NiTi clips are a promising tool in veterinary gastrointestinal surgery. Keywords: CAC, shape memory implants, intestinal anastomosis Journal: Veterinární medicína Pages: 124-128 Volume: 59 Issue: 3 Year: 2014 DOI: 10.17221/7379-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7379-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201403-0002.txt Handle: RePEc:caa:jnlvet:v:59:y:2014:i:3:id:7379-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Milanjeet Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India Author-Name: Harkirat Singh Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India Author-Name: N.K. Singh Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India Author-Name: N.D. Singh Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India Author-Name: Chanchal Singh Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India Author-Name: S.S. Rath Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India Title: Molecular prevalence and risk factors for the occurrence of canine monocytic ehrlichiosis Abstract: Evaluation of blood samples collected from 214 dogs from Ludhiana, Punjab (India) was performed for the presence of Ehrlichia canis using PCR-based assays targeting a portion of the 16S rRNA gene. Of the total samples subjected to routine blood smear examination, the morulae of E. canis were detected in 2.34% samples. Nested PCR assay produced amplicons of expected size (389 bp) specific for E. canis in 41.59% (89/214) of samples. The results of multivariate analysis showed that the prevalence of E. canis was higher in the summer as compared to the winter season (P = 0.031) and in dogs younger than six-month-old as compared to older dogs (P < 0.001), while breed and sex of the host were not significantly associated with the occurrence of the disease. Keywords: canine monocytic ehrlichiosis, Ehrlichia canis, haematological abnormalities, nested PCR, risk factors Journal: Veterinární medicína Pages: 129-136 Volume: 59 Issue: 3 Year: 2014 DOI: 10.17221/7380-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7380-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201403-0003.txt Handle: RePEc:caa:jnlvet:v:59:y:2014:i:3:id:7380-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Melkova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Author-Name: P. Cizek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Author-Name: E. Ludvikova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Author-Name: B. Bezdekova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Title: Equine grass sickness in the Czech Republic a case report Abstract: Equine grass sickness (EGS) is a degenerative polyneuropathy affecting postganglionic parasympathetic and sympathetic neurons. The major clinical signs relate to dysfunction of the gastrointestinal tract and the condition is frequently fatal. EGS has been reported in different parts of the world including Europe. This paper describes the first case of equine dysautonomia in the Czech Republic. The ante mortem diagnosis was based on typical clinical signs and a positive phenylephrine eye-drop test and was confirmed at necropsy following observation of pathognomic histopathological lesions in the enteric neural system of the ileum. Keywords: equine dysautonomia, dysphagia, Czech Republic, horse Journal: Veterinární medicína Pages: 137-140 Volume: 59 Issue: 3 Year: 2014 DOI: 10.17221/7381-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7381-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201403-0004.txt Handle: RePEc:caa:jnlvet:v:59:y:2014:i:3:id:7381-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A.F. Ahmed Author-Workplace-Name: Faculty Veterinary Medicine, Assiut University, Assiut, Egypt Title: Surgical correction of bilateral polydactyly in a dromedary camel: a case report Abstract: The following case report describes the diagnosis and surgery of bilateral polydactyly of unknown origin in a dromedary camel. A two-year-old, 290 kg, intact female camel was admitted for surgical removal of bilateral supernumerary digits associated with the medial aspects of the metacarpi and proximal to the fetlock joints. Radiographic examination revealed bilateral polydactyly with complete fusion of metacarpal bones. Surgery consisted of an osteotomy of surplus metacarpal bones combined with amputation of the supernumerary digits. The follow-up at 12 months after surgery revealed a sound camel with an excellent cosmetic outcome. Keywords: supernumerary, digit, congenital, anomaly, single-humped, Camelus dromedarius Journal: Veterinární medicína Pages: 141-145 Volume: 59 Issue: 3 Year: 2014 DOI: 10.17221/7382-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7382-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201403-0005.txt Handle: RePEc:caa:jnlvet:v:59:y:2014:i:3:id:7382-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Y. Zhalniarovich Author-Workplace-Name: Department of Surgery and Radiology University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: Z. Adamiak Author-Workplace-Name: Department of Surgery and Radiology University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: P. Holak Author-Workplace-Name: Department of Surgery and Radiology University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: P. Przyborowska Author-Workplace-Name: Department of Surgery and Radiology University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: A. Pomianowski Author-Workplace-Name: Department of Internal Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Title: Diagnosis of a brachial plexus tumour using magnetic resonance imaging assisted by fine-needle aspiration biopsy in a dog: a case report Abstract: This study describes a case of a six-year-old female crossbreed dog that had a three months history of progressive right thoracic limb lameness. Palpation revealed muscle atrophy of the scapular and humeral area and painfulness in this region. Magnetic resonance imaging was performed in T1- and T2-weighted and STIR (short inversion recovery) sequences in sagittal and transverse planes. The masses around the scapula and humerus were heterogeneously hyper-intense in STIR and measured about 9 cm. The tumour was heterogeneously iso-intense relatively to the surrounding tissues in T-2 weighted Gradient Echo and Spin Echo sequences. After MRI fine needle aspiration of a central part of the tumour was performed. The biopsy revealed multiple round cells with very little cytoplasm and fusiform cells in which the cytoplasm created protrusions. Cells were arranged in rows or formed a homogeneous cell mass. A malignant peripheral nerve sheath tumour was diagnosed cytologically. Keywords: magnetic resonance imaging, brachial plexus, tumour, fine needle biopsy, dog Journal: Veterinární medicína Pages: 146-149 Volume: 59 Issue: 3 Year: 2014 DOI: 10.17221/7383-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7383-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201403-0006.txt Handle: RePEc:caa:jnlvet:v:59:y:2014:i:3:id:7383-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Pisoni Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: S. Del Magno Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: F. Cinti Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: M. Baron Toaldo Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: M. Joechler Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: M. Pietra Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Title: Combined surgical and endoscopic approach for the reduction of a congenital hiatal hernia in a cat: a case report Abstract: A case of surgical resolution of type I or "sliding" hiatal hernia is reported. A seven-month-old kitten was presented because of abdominal discomfort, accelerated breathing after eating and chronic vomiting. The clinical examination was unremarkable. Thoracic radiographs and gastroscopy led to the diagnosis of type I hiatal hernia. The surgical resolution consisted of hiatal plication, oesophagopexy and left-flank incisional gastropexy. All procedures were carried out using a 6 mm videoendoscope positioned in the stomach to evaluate the right oesophago-gastric junction reduction. One week after surgery there was a recurrence of symptoms and a second laparotomy was performed. During the second surgery additional hiatal plication was necessary and an oesophagopexy was repeated after dissection of the phrenico-oesophageal ligament. Moreover, a new incisional gastropexy was carried out after resolution of the first one. The cat recovered without complications and at one-year follow-up did show no signs related to the hiatal hernia. This communication reports on possible additional surgical techniques in cases of type I hiatal hernia and contributes to an understanding of the importance of oesophagopexy in cases of hiatus malformation. Keywords: cat, surgery, endoscopy, hiatal hernia Journal: Veterinární medicína Pages: 150-156 Volume: 59 Issue: 3 Year: 2014 DOI: 10.17221/7385-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7385-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201403-0007.txt Handle: RePEc:caa:jnlvet:v:59:y:2014:i:3:id:7385-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Pisoni Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: F. Cinti Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: A. Gallucci Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: A. Diana Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: S. Del Magno Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: E. Bellei Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Author-Name: G. Gandini Author-Workplace-Name: School of Agriculture and Veterinary Medicine, University of Bologna, Bologna, Italy Title: Dura mater marsupialisation and outcome in a cat with a spinal subarachnoid pseudocyst: a case report Abstract: A six-month-old male domestic shorthair cat was referred with a history of acute-onset paraplegia, over the previous two months. The neurological examination revealed a thoracolumbar lesion. After myelography and myelo-computed tomography (myelo-CT), the diagnosis of a T13-L1 subarachnoid pseudocyst potentially related to a previous L1 vertebral body fracture or malformation was made. Surgical decompression consisted in dorsal laminectomy followed by durotomy and marsupialisation. Immediately after surgery the cat improved neurologically and showed progressive improvement of his neurological signs over the next few months, until he died, from unrelated causes, approximately 18 months after surgery. Keywords: cat, subarachnoid pseudocyst, marsupialisation, myelo-CT, myelography Journal: Veterinární medicína Pages: 157-161 Volume: 59 Issue: 3 Year: 2014 DOI: 10.17221/7386-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7386-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201403-0008.txt Handle: RePEc:caa:jnlvet:v:59:y:2014:i:3:id:7386-VETMED