Template-Type: ReDIF-Article 1.0 Author-Name: M. Zendehdel Author-Workplace-Name: Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Author-Name: Z. Torabi Author-Workplace-Name: Faculty of Basic Sciences, Islamic Azad University of Damghan, Damghan, Iran Author-Name: S. Hassanpour Author-Workplace-Name: Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran Title: Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test: role of opioidergic and histaminergic systems Abstract: Bunium persicum (Boiss.) is an economically important medicinal plant growing wildly in arid regions in Iran. The fruit of B. persicum is widely used in traditional Iranian medicine to control colic pain and dysmenorrhoea. The aim of the current study was to determine antinociceptive mechanisms of B. persicum essential oil using an acetic acid-induced writhing test as a model of visceral pain and to determine the possible involvement of opioidergic, serotoninergic and histaminergic systems on antinociceptive mechanisms of B. persicum in male mice. In experiment 1, B. persicum was intraperitoneally (i.p.) injected (0.001, 0.01, 0.05, 0.1, 0.5 and 1%; 10 ml/kg) in Tween-80 (0.5%) and a writhing test served as a model of visceral pain. In experiments 2-5, opioidergic receptor antagonist (naloxone, 2 mg/kg), serotonergic receptor antagonist (cyproheptadine, 4 mg/kg), histamine H1-receptor antagonist (chlorpheniramine, 20 mg/kg) and histamine H2-receptor antagonist (cimetidine, 12.5mg/kg) injection was followed by B. persicum (0.01%; 10 ml/kg) and the writhing test response was measured for 30 min. According to the results, essential oil of B. persicum, administered i.p. (0.001, 0.01, 0.05, 0.1, 0.5, and 1%; 10 ml/kg) in Tween-80 (0.5%), elicited antinociceptive effects in a dose-dependent manner. Moreover, the antinociceptive effect of B. persicum was significantly attenuated by pre-treatment with naloxone, chlorpheniramine and cimetidine (P < 0.001). These results suggest that B. persicum-induced analgesia may be mediated via opioidergic and histamine H1 and H2 receptors. Keywords: Bunium persicum, antinociception, writhing test, mouse Journal: Veterinární medicína Pages: 63-70 Volume: 60 Issue: 2 Year: 2015 DOI: 10.17221/7988-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7988-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201502-0001.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:2:id:7988-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.O. Alekish Author-Workplace-Name: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan Title: The association between the somatic cell count and isolated microorganisms during subclinical mastitis in heifers in Jordan Abstract: Mastitis is an important cause of early culling of dairy heifers. The aims of this study were to identify microorganisms that cause subclinical mastitis (SCM) in Holstein heifers in northern Jordan, to estimate the relative importance of various microorganisms, and to examine the correlation between the somatic cell count (SCC) and the isolated microorganisms. Composite milk samples were collected aseptically from 133 Holstein heifers between Days 5 and 14 postpartum for bacterial culture and somatic cell counts. The prevalence of subclinical mastitis in this study was 57.1%. Seventy six (76) of the 133 cows tested had SCC of > 250 000 cells/ml. Bacteria were isolated from 36 (27.1%) cows. About sixty six percent (66.7%) of these isolates were cultured from cows with subclinical mastitis. Coagulase-negative Staphylococci (CNS) were the most prevalent bacteria among isolates (50%), followed by Staphylococcus aureus (22.2%), Corynebacterium bovis and coliforms (E. coli and Klebsiella pneumoniae) (5.6% each), and Streptococcus uberis (2.8%). Interestingly, the majority of the negative-culture samples (53.6%) had elevated SCC. All isolates except for the CNS group exhibited strongly elevated SCC (> 1 × 106cells/ml). However, the coagulase-negative Staphylococci did not have a distinct pattern with respect to SCCs. There was a strong correlation (P ≤ 0.05, correlation coefficient 0.213) between the results of bacterial culture (culture-positive vs. culture-negative) and SCC class but not between SCC class and the type of isolated bacteria. It is concluded that subclinical mastitis appears to be a significant health issue for dairy heifers in Jordan. Keywords: subclinical mastitis (SCM), somatic cell count (SCC), heifers, aetiology, prevalence, Jordan Journal: Veterinární medicína Pages: 71-76 Volume: 60 Issue: 2 Year: 2015 DOI: 10.17221/7980-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7980-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201502-0002.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:2:id:7980-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.M. Hossain Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Choongnam, Republic of Korea Author-Name: M. Begum Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Choongnam, Republic of Korea Author-Name: I.H. Kim Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Choongnam, Republic of Korea Title: Effect of Bacillus subtilis, Clostridium butyricum and Lactobacillus acidophilus endospores on growth performance, nutrient digestibility, meat quality, relative organ weight, microbial shedding and excreta noxious gas emission in broilers Abstract: This study was conducted to evaluate the effects of Bacillus subtilis, Clostridium butyricum and Lactobacillus acidophilus (tri-strain probiotics, TSP) endospores in broilers. TSP can benefit the host animal by increasing nutrient absorption from the gastrointestinal tract and altering the intestinal ecosystem in poultry. A total of 500 day-old ROSS 308 mixed sex broiler chickens with an average initial body weight (IBW) of 45 g ± 0.6 g were used in this 35-day feeding study. Broiler chickens were randomly allotted to one of five dietary treatments: (1) CON (antibiotic free diet), (2) ANT1 (CON + enramycin 5 ppm), (3) ANT2 (CON + avilamycin 5 ppm), (4) TSP1 (CON + 0.1% TSP), and (5) TSP2 (CON + 0.2% TSP) with five replicates per treatment and 20 chicks per pen. Broiler chickens fed on TSP diets exhibited linearly increasing body weight gain (BWG) and decreased feed conversion ratio (FCR) compared to those on the CON diet (P < 0.05; Day 21 to Day 35 and Day 1 to Day 35, respectively). Further, dry matter (DM) and nitrogen (N) digestibility were improved (P < 0.05) in the TSP treatment at the end of study. The inclusion of TSP reduced (linear, P = 0.02) meat lightness (L*) compared with CON and ANT treatments. Broiler chickens fed with TSP diets had relatively higher (linear, P < 0.05) bursa weight than those fed with ANT and CON diets. The supplementation of TSP increased (P < 0.05) the ileal and caecal Lactobacillus count compared with CON and ANT diets. The ileal and caecal Escherichia coli and caecal Clostridium perfringens counts were reduced (P < 0.05) in the TSP2 group compared with the CON group. Broiler chickens fed with TSP diets exhibited increased (P < 0.05) caecal Bifidobacteria counts compared with CON and ANT diets. Excreta ammonia (NH3) gas emission was lower (P < 0.05) with the TSP treatment compared with the CON treatment. In conclusion, the supplementation of TSP improved growth performance, nutrient digestibility, meat quality, gut health and reduced noxious gas emission in broilers. Keywords: ammonia gas emission, body weight gain, bursa weight, Escherichia coli, Lactobacillus, meat lightness Journal: Veterinární medicína Pages: 77-86 Volume: 60 Issue: 2 Year: 2015 DOI: 10.17221/7981-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7981-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201502-0003.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:2:id:7981-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Theodosiadou Author-Workplace-Name: Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece Author-Name: T. Tsiligianni Author-Workplace-Name: Veterinary Research Institute of Thessaloniki, Thessaloniki, Greece Title: Determination of the proper time for mating after oestrous synchronisation during anoestrous or oestrous by measuring electrical resistance of cervical mucus in ewes Abstract: The purpose of the present study was to investigate whether the electrical resistance values of the cervical mucus and/or blood οestradiol-17β and progesterone concentrations at mating, after oestrous synchronisation during the anoestrous or oestrous period in ewes, are related to fertility. Oestrus was induced by medroxyprogesterone acetate-impregnated intravaginal sponges (Veramix, Upjohn, USA), either for 12 days (12-day group; 24 Kymi and 28 Chios) or for 14 days (14-day group; 24 Kymi and 28 Chios), plus 400 IU equine chorionic gonadotropin (Intergonan, Intervet, the Netherlands) i.m., at the time of sponge removal. In each case, one blood sample was collected from all ewes at sponge insertion, at sponge removal and at oestrus, for the determination of serum οestradiol-17β and progesterone concentrations, using a radioimmunoassay. The electrical resistance of the cervical mucus was measured once, just after oestrus detection by teaser rams, using a digital heat detector. All ewes found to be in oestrus were mated to fertile rams. Pregnancy was determined by transabdominal ultrasonography 35-40 days after mating. Kymi ewes were in anoestrous, while those of Chios were in the oestrous period. The 14-day treatment resulted in better outcome as concerns oestrus detection and pregnancy rate than the 12-day treatment, in either oestrous or anoestrous period ewes. Oestradiol-17β concentrations at sponge removal and at oestrus were lower (P < 0.05) in the 14-day group than in the 12-day group, in both oestrous or anoestrous period ewes. Progesterone concentrations at sponge insertion and removal were higher (P < 0.05), while progesterone concentrations and electrical resistance values of the cervical mucus at oestrus were lower (P < 0.05) in ewes of both groups who conceived compared to those that did not, either in oestrous or in the anoestrous period. Linear regression analysis revealed a positive relation between the electrical resistance values of the cervical mucus and blood serum progesterone concentrations in both oestrous or anoestrous period ewes. The electrical resistance of cervical mucus could be useful for the detection of the proper time for mating after oestrous synchronisation in ewes. Keywords: electrical resistance of cervical mucus, oestradiol-17β , progesterone, anoestrous period, oestrous period, ewes Journal: Veterinární medicína Pages: 87-93 Volume: 60 Issue: 2 Year: 2015 DOI: 10.17221/7982-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7982-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201502-0004.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:2:id:7982-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Rauser Author-Workplace-Name: Small Animal Clinic, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Crha Author-Workplace-Name: Small Animal Clinic, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: A. Sivkova Author-Workplace-Name: Small Animal Clinic, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: J. Zapletalova Author-Workplace-Name: Small Animal Clinic, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: N. Korytarova Author-Workplace-Name: Small Animal Clinic, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Influence of atracurium on selected cardiovascular and respiratory variables and surgical times in dogs undergoing laparoscopic ovariectomy with standardized ventilation pattern Abstract: Respiratory and cardiovascular changes in normocapnic dogs undergoing laparoscopic ovariectomy are described in this report. To-date, changes during a standardised ventilation pattern, irrespective of end-tidal CO2 (EtCO2) levels, have not been reported. This study was undertaken to describe these changes. Forty bitches undergoing laparoscopic ovariectomy were enrolled in a prospective double-blind randomised clinical trial. They were first anaesthetised with medetomidine-butorphanol-propofol and then maintained with isoflurane in oxygen-air. Ventilation pattern was pressure-controlled, volume-limited. After stabilisation, atracurium 0.4 mg/kg (ATR group, n = 20) or saline 0.04 ml/kg (SAL group, n = 20) was administered intravenously. Capnoperitoneum was then established with an intra-abdominal pressure setting of 10 mmHg. Collected data included heart rate (HR), non-invasive blood pressure, EtCO2, oxygen saturation of haemoglobin, and tidal volume and neuromuscular blockade (train-of-four). Data were recorded before administration of atracurium or saline, and at 2, 5, and 10 min thereafter. Subsequently, insufflation of CO2 was commenced. After capnoperitoneum was established, data were recorded at 2, 5 and 10 min. Time of capnoperitoneum induction and duration, time of ovary searching and excision and total time of capnoperitoneum and surgery were also recorded. For statistical analysis, the Shapiro Wilk test, ANOVA with repeated measures and Student's t-test for independent samples were used (P < 0.05). Significant increases in EtCO2 were observed in patients 5 min after atracurium administration and 5 min after capnoperitoneum was established in the ATR group, when compared to the SAL group. No other differences were found. Administration of atracurium in dogs undergoing laparoscopic ovariectomy with a standardised ventilation pattern did not result in clinically important differences in selected cardiovascular and respiratory variables and surgical times in comparison with non-relaxed dogs. Thus, the use of atracurium in laparoscopic ovariectomy does not result in any benefit for patients or surgeons. Keywords: bitch, capnoperitoneum, spaying, relaxation Journal: Veterinární medicína Pages: 94-100 Volume: 60 Issue: 2 Year: 2015 DOI: 10.17221/7983-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7983-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201502-0005.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:2:id:7983-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Kriz Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Makovcova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Skoric Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: O. Huml Author-Workplace-Name: VEDILAB Ltd., Plzen, Czech Republic Author-Name: J. Pokorny Author-Workplace-Name: Zoological and Botanical Garden Plzen and Veterinary Office StarVet, Stary Plzenec, Czech Republic Title: Avian mycobacteriosis in an individual of the endangered Mauritian Pink pigeon (Nesoenas mayeri) species: a case report Abstract: Avian mycobacteriosis was diagnosed in one female Mauritian Pink pigeon (Nesoenas mayeri) from a zoological collection. The bird displayed clinical symptoms of apathy, loss of body weight, ruffled feathers and diarrhoea. Necropsy revealed nodular lesions in the internal organs. Mycobacterium avium subsp. avium was isolated and also directly detected using qPCR in the female's liver. Avian mycobacteriosis may pose a threat to efforts directed towards conservation of this endangered species. Keywords: Columba, conservation, dove, tuberculosis Journal: Veterinární medicína Pages: 101-104 Volume: 60 Issue: 2 Year: 2015 DOI: 10.17221/7984-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7984-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201502-0006.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:2:id:7984-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.J. Gabriel Filho Author-Workplace-Name: Veterinary Cardiology Institute, Ribeirao Preto, Brazil Author-Name: G.M. Magalhaes Author-Workplace-Name: University of Franca, Franca, Brazil Author-Name: V.A. Conforti Author-Workplace-Name: University of Franca, Franca, Brazil Author-Name: J. Santilli Author-Workplace-Name: University of Franca, Franca, Brazil Author-Name: S.G. Calazans Author-Workplace-Name: University of Franca, Franca, Brazil Title: Biphasic pericardial and pleural mesothelioma in a cat: a case report Abstract: An 11-year-old castrated male Persian cat was admitted to the veterinary clinic showing signs of lethargy, lack of appetite, laboured breathing, dyspnoea, and cyanosis. The echocardiogram showed a large amount of pleural effusion, fibrin, and a mass measuring 1.89 cm in diameter adhered to the parietal pericardium. Upon necropsy, several nodules were observed on the pericardium sac and pleura. Histological evaluation of the nodules and mediastinal lymph node revealed the presence of neoplastic cells of epithelial and mesenchymal origin. Immunolabelling was positive for both cytokeratin AE1/AE3 and vimentin in the primary tumour and in the mediastinal lymph node. In the present report, immunohistochemical analysis supported the diagnosis that had been obtained based on clinical signs, histopathological examination, and echocardiogram. Keywords: oncology, feline, effusion Journal: Veterinární medicína Pages: 105-108 Volume: 60 Issue: 2 Year: 2015 DOI: 10.17221/7985-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7985-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201502-0007.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:2:id:7985-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Vignoli Author-Workplace-Name: Veterinary Clinic Modena Sud, Spilamberto, Italy Author-Name: L. Stehlik Author-Workplace-Name: Small Animal Clinic, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: R. Terragni Author-Workplace-Name: Veterinary Clinic Modena Sud, Spilamberto, Italy Author-Name: L. Cavallo Author-Workplace-Name: Veterinary Clinic Modena Sud, Spilamberto, Italy Author-Name: P. Proks Author-Workplace-Name: Small Animal Clinic, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Title: Computed tomography-guided cementoplasty combined with radiation therapy for an aneurysmal bone cyst in a dog: a case report Abstract: Aneurysmal bone cysts are expansile osteolytic bone lesions that can manifest clinically as lameness, pain and swelling. In humans, aneurysmal bone cysts are considered benign tumours that have been classified as primary or secondary to a previous coexisting lesion. Local recurrence after treatment or malignant transformation has been reported in both humans and in a dog. Different treatment options have been proposed. This report describes the treatment of an 11-month-old Irish wolfhound with an aneurysmal bone cyst with a combination of computed tomography-guided cementoplasty and radiation therapy, 30 Gy delivered in 10 fractions. The dog recovered completely, with no lameness observed 30 months after treatment. Keywords: lameness, CT, benign bone lesion, osteolytic lesion, polymethylmethacrylate Journal: Veterinární medicína Pages: 109-114 Volume: 60 Issue: 2 Year: 2015 DOI: 10.17221/7986-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7986-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201502-0008.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:2:id:7986-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H.J. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: E.J. Choi Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: H.R. Lee Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: B.T. Kwon Author-Workplace-Name: Taejong Animal Hospital, Busan, Republic of Korea Author-Name: S.H. Do Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Title: Cutaneous extrarenal rhabdoid tumor in a dog: a case report Abstract: Rhabdoid tumours (RTs) are rare, highly aggressive tumours of undetermined origin in humans, and are sub-classified as renal/extrarenal RTs depending on location. The origins of extrarenal rhabdoid tumours are an enigma and neoplasms have rarely been reported in non-primate species. An 11-year-old male Maltese dog was presented with a submandibular mass. Histologically, the mass was composed of sheets of highly pleomorphic "rhabdoid" cells, further characterised by the presence of large epithelioid cells with globular/fibrillar paranuclear inclusions. Further immunohistochemical analysis revealed that the neoplastic cells were positive for vimentin and desmin similar to human tumours. In addition, ultrastructural analysis showed that the intracytoplasmic inclusions were mainly composed of whorled bundles of intermediate filaments. Our results suggest a useful diagnostic approach to cutaneous, extrarenal rhabdoid tumours in dogs and describe their characteristics. Keywords: rhabdoid tumour, cutaneous, cytoplasmic inclusion, vimentin, transmission electron microscopy Journal: Veterinární medicína Pages: 115-119 Volume: 60 Issue: 2 Year: 2015 DOI: 10.17221/7987-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7987-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201502-0009.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:2:id:7987-VETMED