Template-Type: ReDIF-Article 1.0 Author-Name: P. Langova Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Workplace-Name: Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: J. Stembirek Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Workplace-Name: University Hospital Ostrava, Ostrava, Czech Republic Author-Name: E. Matalova Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Buchtova Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Workplace-Name: Faculty of Medicine, Masaryk University, Brno, Czech Republic Title: Tooth autotransplantations - lessons from animal models: a review Abstract: Autotransplantation of teeth is the most natural technique to for replacing missing teeth in exposed parts of the dental arch. Reports from human patients indicate great progress toward successful transplantations. However, complications such as inflammation and ankylosis still occur. To understand regenerative processes after autotransplantations, several animal models have been used (monkeys, rodents, rabbits, cats and dogs) and histological/molecular methods have been established. This review aims to summarise knowledge from animal models and discuss their advantages or disadvantages with respect to possible usage in research. Keywords: revascularisation, reinnervation, ankyloses, mouse, cat, dog, rabbit Journal: Veterinární medicína Pages: 293-300 Volume: 60 Issue: 6 Year: 2015 DOI: 10.17221/8243-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8243-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201506-0001.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:6:id:8243-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. El-Ashker Author-Workplace-Name: Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt Author-Name: M. Salama Author-Workplace-Name: Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt Author-Name: M. El-Sebaei Author-Workplace-Name: Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt Author-Name: E. Risha Author-Workplace-Name: Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt Author-Name: F. Abdelhamid Author-Workplace-Name: Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt Author-Name: M. El-Diasty Author-Workplace-Name: Animal Health Research Institute, Mansoura Provincial Laboratory, Mansoura, Egypt Author-Name: E. El-Fadle Author-Workplace-Name: Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt Title: Significance of clinical variables and selected biochemical markers in predicting the outcome of bovine anaplasmosis Abstract: The present study was aimed at evaluating the usefulness of selected inflammatory and oxidative stress markers in predicting the clinical outcome of cattle infected with Anaplasma (A). marginale. The study population consisted of 39 cattle naturally infected with A. marginale. The presumptive diagnosis of this infection was initially achieved on the basis of case history, microscopy and clinical examination findings, and confirmed using A. marginale-specific PCR assays. The diseased cattle were categorised according to the clinical outcome into survivors (n = 26) and non-survivors (n = 13). For comparison, ten clinically healthy cattle were randomly selected and served as controls. Blood was drawn from all examined animals to measure the respective levels of selected cytokines, acute phase proteins, oxidative stress markers, and antioxidant enzyme levels. We found that the clinical examination alone was not conclusive and should be used in conjunction with other diagnostic methods. Nonetheless, the non-surviving animals showed anorexia, frequent coughing, dyspnoea, bloody faeces, recumbency, pale or icteric mucous membranes, and haemoglobinuria. Biochemically, tumour necrosis factor alpha, interleukin (IL)-1β, IL-6, serum amyloid A, fibrinogen, malondialdehye, superoxide dismutase, and catalase levels were significantly higher in diseased cattle compared with controls, and were higher in non-survivors than survivors (P < 0.05). In contrast, reduced glutathione (G-SH) was significantly lower in non-surviving cattle than survivors and controls. Interestingly, a significant correlation was found between parasitaemia of the diseased cattle and most of the measured biochemical variables, with IL-1β and G-SH showing the highest correlation. Our findings clearly demonstrate that A. marginale infection is associated with marked inflammatory and oxidative stress responses, which are higher in non-surviving cattle compared with survivors. The overall degree of cytokine and anti-oxidative disruption may have an important prognostic value for the disease outcome. Keywords: Anaplasma marginale, cytokine profile, oxidative stress, prognosis, cattle Journal: Veterinární medicína Pages: 301-308 Volume: 60 Issue: 6 Year: 2015 DOI: 10.17221/8244-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8244-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201506-0002.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:6:id:8244-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: G. Turk Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: M. Yuksel Author-Workplace-Name: Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Turkey Author-Name: M. Sonmez Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: S. Gur Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: S. Ozer Kaya Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: E. Demirci Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Title: Effects of semen sexing kits (HeiferplusTM and BullplusTM) supplemented to frozen-thawed bull semen on pregnancy rates, foetal sex ratios and selected reproductive parameters in cows Abstract: It has been claimed that artificial insemination (AI) of cows with frozen-thawed semen treated with commercially produced kits, HeiferplusTM (HP, in favour of female gender) and BullplusTM (BP, in favour of male gender), increases the birth chance of calves with desired sex ratio by at least 20-25% and pregnancy rates by at least 5-20%. Hence, this study was conducted to investigate the efficacy of HP and BP kits as combined with AI on the pregnancy rates, foetal sex ratios and some reproductive parameters in cows. For this, a total of 200 cows (100 Holsteins and 100 Simmentals) from three to five years old were used. Fifty Holstein and 50 Simmental cows served as controls. The other half of Holstein and Simmental cows was artificially inseminated with frozen-thawed semen treated with HP and BP, respectively. Findings showed that the AI of cows with frozen-thawed semen treated with HP had no significant effect on the pregnancy rate [52.0% (26/50) in HP group; 56.0% (28/50) in control group], female calf ratio [52.0% (13/25) in HP group; 44.4% (12/27) in control group], embryonic death, abortion, stillbirth, twinning and gestation length as compared to the control group. Similarly, AI of cows with frozen-thawed semen treated with BP did not lead to any significant effect on the pregnancy rate [64.0% (32/50) in BP group; 58.0% (29/50) in control group], male calf ratio [53.1% (17/32) in BP group; 39.3% (11/28) in control group] and other reproductive parameters as compared to the control group. In conclusion, HP and BP treatments of semen used in the AI provided only slight, non-significant increases in female (7.6%) and male (13.8%) calf ratios, respectively. Keywords: Heiferplus, Bullplus, foetal sex, pregnancy rate, cow Journal: Veterinární medicína Pages: 309-313 Volume: 60 Issue: 6 Year: 2015 DOI: 10.17221/8245-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8245-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201506-0003.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:6:id:8245-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.X. Zhang Author-Workplace-Name: Agricultural University of Hebei, Baoding, P.R. China Author-Name: K. Fu Author-Workplace-Name: Northeast Agricultural University, Harbin, P.R. China Author-Name: X.Y. Chi Author-Workplace-Name: Northeast Agricultural University, Harbin, P.R. China Author-Name: J.T. Zhang Author-Workplace-Name: Northeast Agricultural University, Harbin, P.R. China Author-Name: L. Gao Author-Workplace-Name: Northeast Agricultural University, Harbin, P.R. China Author-Name: H.B. Wang Author-Workplace-Name: Northeast Agricultural University, Harbin, P.R. China Title: Laparoscopic abomasal cannulation in sheep Abstract: Abomasal cannulation, an important research tool in experimental procedures, provides a method for the placement of an abomasal cannula in sheep. The aim of this study was to describe a technique for laparoscopic abomasal cannulation in sheep. It was performed in six anaesthetised sheep positioned in dorsal recumbency using three portals in the abdomen. The first absorbable traction suture was placed 1 cm cranial to the determined abomasal cannula site. A stab incision was made with a laparoscopic monopolar hook electrode in the middle of a purse-string suture placed around the abomasal cannula site. The T-shaped cannula was inserted into the abomasum lumen through the stab incision, and the second traction suture was then placed 1 cm caudal to the abomasal cannula site. The T-shaped abomasal cannula was pulled out of the abdominal cavity through the exit wound located 3-5 cm lateral and 10-12 cm cranial to the right side of the umbilicus. The two traction sutures were tied. The T-shaped cannula was secured to the skin with a finger-trap suture. Repeat laparoscopy was performed 1 month later. Firm adhesion between the abomasum and abdominal wall was observed in all sheep, with no evidence of leakage or peritonitis. No major intraoperative or postoperative complications were encountered. The median surgical time was 49 min, with a range from 42 to 58 min. The abomasal contents were collected easily. In conclusion, laparoscopic abomasal cannulation is safe and easy to perform. Its low complication rates and the "ideal" placement of the tube into the abomasum make it an especially attractive alternative to traditional surgical abomasal cannulation in veterinary practice. Keywords: laparoscopy, ewes, abomasum, T-shaped abomasal cannula Journal: Veterinární medicína Pages: 314-322 Volume: 60 Issue: 6 Year: 2015 DOI: 10.17221/8246-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8246-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201506-0004.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:6:id:8246-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Y.O. Birdane Author-Workplace-Name: Faculty of Veterinary Medicine, University of Afyon Kocatepe, Afyonkarahisar, Turkey Author-Name: F.M. Birdane Author-Workplace-Name: Faculty of Veterinary Medicine, University of Afyon Kocatepe, Afyonkarahisar, Turkey Title: Pharmacokinetics of florfenicol following intravenous and intramuscular administration in dogs Abstract: Florfenicol is a synthetic broad-spectrum antibiotic used to treat infectious diseases in veterinary medicine. Limited information is available on the pharmacokinetics and bioavailability of florfenicol in dogs. This study was conducted in six healthy dogs to determine the bioavailability and pharmacokinetics of florfenicol following a single intravenous (i.v.) and intramuscular (i.m.) dose of 30 mg/kg body weight (b.w.). Blood samples were taken over the course of 24 h post-treatment and the recovered plasma was extracted and analysed using high-performance liquid chromatography (HPLC). Pharmacokinetic analysis was performed using a two-compartment open model. After i.v. administration of florfenicol, elimination half-life (t1/2b), volume of distribution at steady state (Vdss), total body clearance (ClT) and area under curve (AUC0-24) were 3.09 ± 0.13 h, 1.19 ± 0.15 l/kg, 0.37 ± 0.04 l/h/kg, and 59.44 ± 5.27 µg/h/ml, respectively. The peak plasma concentration (Cmax), time to maximum concentration (tmax) and bioavailability (F) were 3.05 ± 0.43 µg/ml, 1.50 ± 0.35 h, and 44.70 ± 6.75%, respectively, following i.m. administration. In this study the time that plasma concentration exceed the concentration of 1 µg/ml was approximately 8 h. Therefore, florfenicol should be given twice daily at a dosage of 30 mg/kg b.w. to maintain therapeutic concentration. The pharmacokinetic profile of florfenicol in dogs reveals that it may be therapeutically useful against susceptible microorganisms involved in most common infections in dogs. Keywords: pharmacokinetics, florfenicol, bioavailability, dogs Journal: Veterinární medicína Pages: 323-329 Volume: 60 Issue: 6 Year: 2015 DOI: 10.17221/8247-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8247-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201506-0005.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:6:id:8247-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: C. Roy Author-Workplace-Name: Veterinary Clinic, Riom-es-Montagnes, France Author-Name: N. Vidal-Naquet Author-Workplace-Name: VetoAdom, Montrouge, France Author-Name: B. Provost Author-Workplace-Name: SupAgro University, Montpellier, France Title: A severe sacbrood virus outbreak in a honeybee (Apis mellifera L.) colony: a case report Abstract: A honeybee colony, part of an apiary of nine, showed abnormalities in brood pattern and was thus presented for study. A classic veterinary medicine approach has allowed the diagnosis of a severe case of sacbrood virus (SBV) confirmed by a high viral load in affected larvae. SBV is known to infect larvae of the honeybee (Apis mellifera), resulting in failure to pupate and ultimately death of infected larvae. Several contributing factors combined, among them the parasite Varroa destructor, have been identified in this particular affected colony to explain the clinical outbreak of the disease whereas, in the majority of cases, infected colonies remain asymptomatic. As no specific cure of honeybee viruses is available, the management of these contributing factors is essential, including feeding of colonies and control of the Varroa parasite. After implementation of management solutions, the colony rapidly recovered in six weeks, but did not recommence honey production and remained at higher risk of a winter collapse. An earlier control management would have been more effective: regular visits of the colonies by the beekeepers should be the rule in order to detect abnormalities and also to detect and eliminate as early as possible the combination of factors that contribute to the proliferation of the virus. Keywords: Apis mellifera, contributing factors, synergism, sacbrood virus, Varroa destructor Journal: Veterinární medicína Pages: 330-335 Volume: 60 Issue: 6 Year: 2015 DOI: 10.17221/8248-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8248-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201506-0006.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:6:id:8248-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H.J. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: E.J. Choi Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: H.R. Lee Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: G.J. Park Author-Workplace-Name: Woo-Sin Animal Hospital, Seoul, Republic of Korea Author-Name: E.S. Yun Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: J.H. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: S.H. Do Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Title: Spindle cell lipoma in the gingiva of a dog: a case report Abstract: Spindle cell lipoma is a benign lipomatous tumour that mainly occurs in the subcutis of the head, neck, and shoulder in elderly men. Oral spindle cell lipoma is extremely rare in all species, and no cases have been reported in veterinary medicine. A 10-year-old male Maltese dog was presented for evaluation of a white- to cream-coloured gingival mass. Microscopically, the mass was composed of mature fat cells and spindle cells containing thick fibrocollagenous bundles. The neoplastic cells showed positive immunoreactivity to vimentin and CD34. Based on the clinical and histopathological findings, spindle cell lipoma of the gingiva was diagnosed. Further immunohistochemical analysis revealed that both adipocytes and spindle cells exhibited positive reactions to oestrogen receptors α and β. These findings demonstrate the clinical, morphological, and immunohistochemical characteristics of an oral spindle cell tumour. The potential role of sex steroid hormones in the pathogenesis of this tumour is also discussed. Keywords: oral cavity, spindle cell lipoma, CD34, vimentin, oestrogen receptor Journal: Veterinární medicína Pages: 336-340 Volume: 60 Issue: 6 Year: 2015 DOI: 10.17221/8249-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8249-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201506-0007.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:6:id:8249-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Pietra Author-Workplace-Name: University of Bologna, Ozzano dell'Emilia, Bologna, Italy Author-Name: L. Pisoni Author-Workplace-Name: University of Bologna, Ozzano dell'Emilia, Bologna, Italy Author-Name: N. Linta Author-Workplace-Name: University of Bologna, Ozzano dell'Emilia, Bologna, Italy Author-Name: S. Pinna Author-Workplace-Name: University of Bologna, Ozzano dell'Emilia, Bologna, Italy Author-Name: N. Romagnoli Author-Workplace-Name: University of Bologna, Ozzano dell'Emilia, Bologna, Italy Author-Name: A. Diana Author-Workplace-Name: University of Bologna, Ozzano dell'Emilia, Bologna, Italy Title: Endoscopy-assisted tracheal reconstruction of post-traumatic obstruction in a cat: a case report Abstract: A domestic shorthair cat was referred with a history of dyspnoea and lethargy that had arisen gradually within the last few days. The cat had been hit by an automobile 10 days earlier. A thoracic radiograph suggested stenosis of the intrathoracic trachea, proximal to the tracheal bifurcation. Endoscopic examination confirmed a narrowing of the tracheal lumen due to the presence of a fibrotic ring, with remaining patent lumen of about 2 mm. A surgical treatment with a right lateral thoracotomy approach and resection of the narrowed portion of the trachea (a length of about 1 cm) was performed under endoscopic vision. The procedure was unsuccessful because of the size of the tissue removed and the laxity of the remaining tracheal tissue that caused dehiscence of sutures between the tracheal stump and tracheal bifurcation. After consultation with the cat's owners, an intraoperative euthanasia was performed. Keywords: cat, dyspnoea, endoscopy, radiographic findings, tracheal stenosis Journal: Veterinární medicína Pages: 341-344 Volume: 60 Issue: 6 Year: 2015 DOI: 10.17221/8250-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8250-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201506-0008.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:6:id:8250-VETMED