Template-Type: ReDIF-Article 1.0 Author-Name: A. Palanova Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic Title: Collie eye anomaly: a review Abstract: Collie eye anomaly (CEA) is an inherited congenital visual impairment with heterogeneous signs. The first symptoms are already visible in the early embryo. Among the most affected breeds are Collies and Shetland Sheepdogs but the disease has spread to different breeds depending on the country of origin. Dogs affected with this disease share a 7.8 kb deletion in intron 4 of the NHEJ1 gene. Inheritance of this disease is autosomal recessive with incomplete penetrance. Thanks to a commercially available genetic test breeders can identify genetically affected recessive homozygotes and clinically healthy but genetic carriers of the mutation and thus select healthy parents for the next generation of dogs. However, the exact cause of the disease is not known and it is not known whether the causative mutation influences the occurrence of some other diseases (e.g. immunodeficiencies). Keywords: hereditary, eye, disease, dog Journal: Veterinární medicína Pages: 345-350 Volume: 60 Issue: 7 Year: 2015 DOI: 10.17221/8381-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8381-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201507-0001.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:7:id:8381-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Trckova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: A. Lorencova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Hazova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Sramkova Zajacova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Prophylaxis of post-weaning diarrhoea in piglets by zinc oxide and sodium humate Abstract: The high prophylactic doses of ZnO commonly used to control post-weaning diarrhoea (PWD) in piglets have become an environmental problem. The possibility of partial replacement of ZnO by sodium humate (HNa) in PWD treatment was investigated in this study. Weaned piglets (32) were challenged with two enterotoxigenic E. coli strains (ETEC/O149/F4/LT and ETEC/O147/F18/LT) and allocated into four treatments maintained for three weeks: C (no supplementation); ZnO2.5 (2.5 g ZnO/kg diet); HNa + ZnO1.0; HNa + ZnO1.5; HNa + ZnO1.7 (20 g HNa and 1.0, 1.5 and 1.7 g ZnO/kg diet, respectively). The effects on incidence, severity and duration of diarrhoea, faecal shedding of total E. coli and both ETEC strains, growth rate and selected blood parameters were investigated. In contrast with ZnO2.5 and HNa + ZnO1.7, high daily diarrhoea scores, incidence and duration and mortality due to severe dehydration were seen in C, HNa + ZnO1.0 and HNa + ZnO1.5 groups. The administration of ZnO and HNa did not affect the faecal shedding of the challenged ETEC strains for eght days, even in clinically healthy piglets in ZnO2.5 and HNa + ZnO1.7 groups. Signs of growth depression were found in C; HNa + ZnO1.0 and HNa + ZnO1.5 groups during the first week. No difference in growth performance was observed in ZnO2.5 and HNa + ZnO1.7 piglets. Most of the selected biochemical and haematological parameters did not differ significantly among the treatments. However, a significantly higher serum Zn as a result of high dietary ZnO intake in the ZnO2.5 group compared to the control and HNa groups was detected. Significantly lower serum P in ZnO2.5; HNa + ZnO1.7 and HNa + ZnO1.0 groups compared to the control group was most likely induced by the increased Zn in serum. The results indicate the possibility of reducing the high pharmacological levels of ZnO in the prophylaxis of PWD through partial replacement with HNa. Such a treatment maintains the favourable prophylactic effect while lowering the Zn content in faeces. Keywords: humic, enterotoxigenic E. coli, faecal shedding, growth performance, blood chemistry Journal: Veterinární medicína Pages: 351-360 Volume: 60 Issue: 7 Year: 2015 DOI: 10.17221/8382-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8382-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201507-0002.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:7:id:8382-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Rychlik Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: S. Gonkowski Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: M. Nowicki Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: J. Calka Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Title: Cocaine- and amphetamine-regulated transcript immunoreactive nerve fibres in the mucosal layer of the canine gastrointestinal tract under physiological conditions and in inflammatory bowel disease Abstract: The objective of this study was to determine the effect of canine inflammatory bowel disease (IBD) on the density of cocaine- and amphetamine-regulated transcript (CART) immunoreactive nerve fibres in the mucosa of different sections of the gastrointestinal tract. Fibre density was analysed in mucosal specimens from the duodenum, jejunum and descending colon of healthy dogs and patients with inflammatory bowel disease of varying intensity. Nervous fibres were stained in single-cell immunofluorescence assays. The density of CART-immunoreactive fibres was determined using a semi-quantitative approach by counting the number of fibres in the field of view (0.1 mm2). An increase in the density of CART-immunoreactive fibres was observed in dogs with moderate and severe IBD in comparison with healthy subjects. The results suggest that the presence of CART in enteric nerve fibres could play a role in the pathogenesis and development of canine IBD. Keywords: immunohistochemistry, digestive tract, nervous system, dogs Journal: Veterinární medicína Pages: 361-367 Volume: 60 Issue: 7 Year: 2015 DOI: 10.17221/8383-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8383-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201507-0003.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:7:id:8383-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A.M. Molina Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: M.R. Moyano Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: J.M. Serrano-Rodriguez Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: N. Ayala Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: A.J. Lora Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: J.M. Serrano-Caballero Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Title: Analyses of anaesthesia with ketamine combined with different sedatives in rats Abstract: The goal of this study was to establish an easy-to-perform, reliable, and safe protocol for intraperitoneal injection anaesthesia in rats, as well as an easy method to assess anaesthesia depth under routine clinical conditions. Seventy Wistar rats (35 males and 35 females) were used to evaluate intraperitoneal anaesthesia with ketamine (75 mg/kg) combined with one of the following central nervous system depressors: acepromazine (2.5 mg/kg), diazepam (5 mg/kg), medetomidine (0.5 mg/kg), midazolam (5 mg/kg) and xylazine (2.5 mg/kg). Significant differences were found between the combinations in ataxia, recovery and righting parameters, as well as respiratory and heart rates and haemoglobin saturation by oxygen, whereas significant differences between sexes for the parameters of ataxia, hypnosis, recovery, righting and exploratory behaviour were observed upon waking. The best results for parameters such as induction, and maintenance and recovery from anaesthesia (more regular and stable) were observed with α2-agonists: ketamine/xylazine in males and ketamine/medetomidine in females. Keywords: anaesthesia, rats, ketamine, sedatives Journal: Veterinární medicína Pages: 368-375 Volume: 60 Issue: 7 Year: 2015 DOI: 10.17221/8384-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8384-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201507-0004.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:7:id:8384-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N.M. Vuckovic Author-Workplace-Name: Medical Faculty Novi Sad, University of Novi Sad, Novi Sad, Serbia Author-Name: D.C. Vuckovic Author-Workplace-Name: Medical Faculty Novi Sad, University of Novi Sad, Novi Sad, Serbia Author-Name: M.I. Urosevic Author-Workplace-Name: Institute of Food Technology, University of Novi Sad, Novi Sad, Serbia Author-Name: V.S. Cabarkapa Author-Workplace-Name: Medical Faculty Novi Sad, University of Novi Sad, Novi Sad, Serbia Title: A cartilaginous choristoma in a pig liver: a case report Abstract: Choristomas are small aggregates of normal tissue components in aberrant locations, and may be mistaken for true neoplasms. We report a case of an incidentally found mature cartilage island in the portal tract of a two-month old piglet. All other examined organs were normal. The pig belonged to the control group of animals in a short-term experiment, was fed with wheat and corn, and did not receive any external or internal treatment. To the best of our knowledge, this is the first case of a mature cartilaginous choristoma in pig liver. Keywords: cartilage, choristoma, liver, microscopy, swine Journal: Veterinární medicína Pages: 376-378 Volume: 60 Issue: 7 Year: 2015 DOI: 10.17221/8385-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8385-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201507-0005.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:7:id:8385-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Drazek Author-Workplace-Name: University of Warmia and Mazury, Olsztyn, Poland Author-Name: M. Lew Author-Workplace-Name: University of Warmia and Mazury, Olsztyn, Poland Author-Name: S. Lew Author-Workplace-Name: University of Warmia and Mazury, Olsztyn, Poland Author-Name: J. Szarek Author-Workplace-Name: University of Warmia and Mazury, Olsztyn, Poland Author-Name: I. Balicki Author-Workplace-Name: University of Life Sciences, Lublin, Poland 2Teramo University, Teramo, Italy Author-Name: L. Della Salda Title: Equine ocular squamous cell carcinoma: a case report Abstract: An eight-year-old gelding, Wielkopolski Horse was presented with a pink tumour, which filled the entire palpebral fissure of the left eye. Ultrasonography revealed it to be well-demarcated from the cornea mass, which covered its entire surface. Due to the extensive size of the lesion and the lack of owner's consent to post-operative treatment, it was decided to perform enucleation. A histopathological examination revealed corneal squamous cell carcinoma (CSCC). The primary cause of the tumour has not been established. Immunohistochemistry was negative for p16 immunoreactivity which is typical for the E7 oncoprotein in PV infection, and is suspected of involvement in the aetiopathogenesis of ocular squamous cell carcinoma (OSCC). Keywords: eyeball neoplasm, SCC, p16 protein, horse Journal: Veterinární medicína Pages: 379-386 Volume: 60 Issue: 7 Year: 2015 DOI: 10.17221/8386-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8386-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201507-0006.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:7:id:8386-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N. Orellana-Jaimes Author-Workplace-Name: Department of Veterinary Medicine and Surgery, University of Leon, Leon, Spain Author-Name: M.M. Ginja Author-Workplace-Name: Department of Veterinary Science CITAB, University of Tras-os-Montes e Alto Douro, Vila Real, Portugal Author-Name: F. San Roman-Llorens Author-Workplace-Name: Department of Veterinary Medicine and Surgery, University of Leon, Leon, Spain Author-Name: M. Garcia-Gomez Author-Workplace-Name: Department of Veterinary Medicine and Surgery, University of Leon, Leon, Spain Author-Name: M.A. Orden Author-Workplace-Name: Department of Veterinary Medicine and Surgery, University of Leon, Leon, Spain Author-Name: J.R. Altonaga Author-Workplace-Name: Department of Veterinary Medicine and Surgery, University of Leon, Leon, Spain Author-Name: J.M. Gonzalo-Orden Author-Workplace-Name: Department of Veterinary Medicine and Surgery, University of Leon, Leon, Spain Title: Magnetic resonance imaging: findings of osteochondrosis like-lesions in glenoid fossa and proximal humeral metaphyses in a dog: a case report Abstract: Osteochondritis dissecans (OCD) of the humeral head is a common cause of forelimb lameness in dogs. OCD represents the late phase of osteochondrosis (OC). Magnetic resonance imaging (MRI) is a useful technique for detection and characterisation of this disease. The main objective of this study was to show MRI findings of OCD in the humeral head, OC like-lesions in glenoid fossa and proximal humeral metaphyses of a dog. MRI analysis revealed the extent and severity of the inflammatory changes within the subchondral bones in both the glenoid cavity and humeral head. OCD and OC like-lesions were also evaluated in histopathological studies. Keywords: magnetic resonance imaging (MRI), osteochondritis dissecans (OCD), shoulder, dog Journal: Veterinární medicína Pages: 387-390 Volume: 60 Issue: 7 Year: 2015 DOI: 10.17221/8387-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8387-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201507-0007.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:7:id:8387-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J.H. Kim Author-Workplace-Name: Konkuk University Veterinary Medical Teaching Hospital, Seoul, Republic of Korea Author-Name: W.J. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: J. Park Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: J.I. Shin Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: H.Y. Yoon Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Title: Canine mammary anaplastic carcinoma with concurrent aorto-iliac thrombosis in a dog: a case report Abstract: An 11-year-old, 6.75 kg, spayed female Maltese dog was referred for evaluation of a recurrent mammary gland tumour (MGT) after recent lumpectomies. We performed a regional mastectomy, and the mammary gland tumour was diagnosed as a mammary anaplastic carcinoma. On the 11th postoperative day, the dog presented with a one-day history of lethargy and left hind limb weakness. Increased D-dimer level and two-dimensional and Doppler ultrasonography revealed a unilateral aorto-iliac thrombus. Although prompt thrombolytic drug administration by intravenous infusion was recommended, the owner did not consent to further examination and treatment due to the side effects, and the patient died 24 h after it was diagnosed with arterial thrombosis (AT). This is the first report of a canine mammary anaplastic carcinoma with concurrent arterial thrombosis in a dog. These results suggest that cancer malignancy-induced hypercoagulability should be considered in the differential diagnosis of arterial thrombosis. Keywords: arterial thrombosis, D-dimer, hypercoagulability, malignant, mammary gland tumour, dog Journal: Veterinární medicína Pages: 391-398 Volume: 60 Issue: 7 Year: 2015 DOI: 10.17221/8388-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8388-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201507-0008.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:7:id:8388-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Zeleny Author-Workplace-Name: Institute of Forensic Medicine, St. Anne's University Hospital, Brno, Czech Republic Author-Workplace-Name: Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: K. Grusova Author-Workplace-Name: Institute of Forensic Medicine, St. Anne's University Hospital, Brno, Czech Republic Author-Workplace-Name: Faculty of Medicine, Masaryk University, Brno, Czech Republic Title: A car accident involving a restrained dog within the vehicle: a case report Abstract: The aim of this article is to describe and evaluate a unique car accident involving a pet dog within a car. The unique aspect is the linking and evaluation of information from the veterinary record with a detailed description of the car accident, the driver's injuries, the safety systems used and photographs. This method of complex evaluation of an injury mechanism is commonly used in forensic medicine when evaluating car accidents involving people. The accident is especially unique due to the fact that a dog safety harness was used to restrain the pet dog. The dog suffered severe injuries, whilst the car driver suffered only minor injuries. The conclusion was that the dog safety harness did not work correctly and did not protect the dog. Keywords: safety harness, transportation, trauma, dog Journal: Veterinární medicína Pages: 399-402 Volume: 60 Issue: 7 Year: 2015 DOI: 10.17221/8389-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8389-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201507-0009.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:7:id:8389-VETMED