Template-Type: ReDIF-Article 1.0 Author-Name: B. Kempisty Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Author-Workplace-Name: Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland Author-Name: P. Zawierucha Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Author-Workplace-Name: Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland Author-Name: S. Ciesiolka Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Author-Name: H. Piotrowska Author-Workplace-Name: Department of Toxicology, Poznan University of Medical Sciences, Poznan, Poland Author-Name: P. Antosik Author-Workplace-Name: Institute of Veterinary, Poznan University of Life Sciences, Poznan, Poland Author-Name: D. Bukowska Author-Workplace-Name: Institute of Veterinary, Poznan University of Life Sciences, Poznan, Poland Author-Name: M. Jeseta Author-Workplace-Name: Centre of Assisted Reproduction, Department of Obstetrics and Gynaecology, University Hospital Brno, Czech Republic Author-Name: M. Nowicki Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Author-Name: K.P. Brussow Author-Workplace-Name: Department of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany Author-Name: M. Zabel Author-Workplace-Name: Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland Author-Workplace-Name: Department of Histology and Embryology, Wroclaw University of Medical Sciences, Wroclaw, Poland Title: Developmental competence of mammalian oocytes-insights into molecular research and the promise of microfluidic technology: a review Abstract: Developmental competence of female gametes determines their maturation ability, successful fertilisation, and proper zygote formation. Oocyte quality may be assessed by expression profiling of several gene markers such as Cx43, TGFB, GDF9, BMP, Lox and Pdia5 that determine the biological features of oocytes. Conversely, several other extrinsic factors, including follicular size or heat shock may significantly influence oocyte quality and ability to grow and develop during folliculo- and oogenesis. However, using molecular methods for evaluation of oocyte quality often leads to destruction of an analysed cell. Therefore, there is an increased requirement to seek new non-invasive methods of oocyte-embryo quality assessment. Here we describe the Lab-on-Chip system based on microfluidic technology, which is the first parametric and objective device for evaluation of oocyte developmental competence using spectral images. In this review several extrinsic factors and molecular markers of oocyte developmental competence are discussed. Furthermore, based on our previous studies, we discuss the possibility of applying the spectrophotometric system (Lab-on-Chip) in both biomedical and reproductive research in domestic animals. Keywords: pig, oocyte, developmental capacity, Lab-on-Chip Journal: Veterinární medicína Pages: 663-674 Volume: 60 Issue: 12 Year: 2015 DOI: 10.17221/8580-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8580-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201512-0001.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:12:id:8580-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Mohana Devi Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Chungnam, Republic of Korea Author-Name: S.C. Kim Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Chungnam, Republic of Korea Author-Name: I.H. Kim Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Chungnam, Republic of Korea Title: Evaluation of effect of supplementation of extruded rice as a substitute for dried whey in the diet of weanling pigs Abstract: A total of 120 weanling pigs [(Landrace × Yorkshire) × Duroc], 21 days of age with an average initial body weight (BW) of 6.52 ± 0.22 (SE) kg were selected to investigate the effects of extruded rice product as a replacement for dried whey on growth performance, coefficient of total tract apparent digestibility (CATTD), blood profiles, faecal shedding of Lactobacillus and Escherichia coli (the microbial counts of digesta were expressed as log10 colony forming units per gram), and faecal scores of weanling pigs. Pigs were randomly allocated to one of four dietary treatments, with six replicates per treatment and five pigs per pen. Pigs in the control group were fed a diet based on corn, soybean, and 20% dried whey. Experimental groups received the same diet as the control group, but dried whey was replaced by 3%, 6%, and 9% extruded rice. Throughout the experimental period (six weeks), no differences were observed in the average daily gain, average daily feed intake, and gain/feed ratio. Pigs fed 6% and 9% extruded rice had a lower (P < 0.05) CATTD than pigs fed the control diet on Day 14. The blood creatinine concentration of pigs fed 9% extruded rice was higher (P < 0.05) than that of pigs in the control group on Day 14. Pigs fed the diets containing 6% and 9% extruded rice had decreased (P < 0.05) faecal E. coli counts on Day 14 compared with pigs fed the control diet. The current results indicate that feeding extruded rice can decrease faecal E. coli counts without negative effects on growth performance in weanling pigs. Keywords: extruded rice, weanling pigs, microflora, digestibility Journal: Veterinární medicína Pages: 675-682 Volume: 60 Issue: 12 Year: 2015 DOI: 10.17221/8581-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8581-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201512-0002.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:12:id:8581-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Charuta Author-Workplace-Name: Faculty of Natural Science, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland Author-Name: M.R. Tatara Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland Author-Name: M. Dzierzecka Author-Workplace-Name: Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland Author-Name: E. Polawska Author-Workplace-Name: Institute of Genetics and Animal Breeding PAS, Jastrzebiec, Poland Author-Name: I. Ptaszynska Sarosiek Author-Workplace-Name: Department of Forensic Medicine, Medical University of Bialystok, Bialystok, Poland Title: Interrelationships of body weight and bone weight with densitometric properties of tibiotarsal bone in geese during post-hatching development Abstract: The aim of this study was to evaluate interrelationships of body weight and bone weight and densitometric properties of the tibiotarsus in White Koluda Geese (W31) in the post-hatching period. The study was performed using dual-energy X-ray absorptiometry (DEXA) and peripheral quantitative computed tomography (pQCT) at two different parts of tibia: proximal metaphysis and mid-diaphysis. The investigation was performed on 100 bones obtained from males and females at the age of 1, 14, 28, 42 and 56 days of life. All the calculations were performed using the Statistica 9.0 software (StatSoft, Inc. Tulsa, USA). Pearson's correlation coefficient of body weight and bone weight with all the investigated variables of bone was determined. Depending on the method used for densitometric measurements - DEXA or pQCT, the current study has revealed significant differences in the number of correlations of bone weight and body weight with the evaluated densitometric parameters. Sex-related differences in the investigated interrelationships were also found. In the case of proximal epiphysis, negative correlations of vBMD, tBMC, CTR_DEN and CRT_CNT with body weight and bone weight dominated in one-day-old males. Based on the current observations and the negative correlations of body weight and vBMD, CRT_DEN and TRAB_DEN obtained in the mid-diaphysis of tibiotarsus at the age of 14 days of life, it was concluded that this bone is much more prone to deformations and fractures in males than in females. Keywords: densitometry parameters, tibiotarsal bone, mineralisation, geese Journal: Veterinární medicína Pages: 682-690 Volume: 60 Issue: 12 Year: 2015 DOI: 10.17221/8582-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8582-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201512-0003.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:12:id:8582-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Fazio Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: G. Piccione Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: C. Saoca Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: A.R. Caputo Author-Workplace-Name: Council for Research and Experimentation in Agriculture, Research Unit for Livestock Extensive, Potenza. Italy Author-Name: S. Cecchini Author-Workplace-Name: Department of Sciences, University of Basilicata, Potenza, Italy Title: Assessment of oxidative stress in Flathead mullet (Mugil cephalus) and Gilthead sea bream (Sparus aurata) Abstract: In this work we compared two species of fish with different feeding habits: Flathead mullet (Mugil cephalus) and Gilthead sea bream (Sparus aurata). The aim of this study was to evaluate total oxidant status (TOS), total antioxidant capacity (TAC) and TOS/TAC ratio (OSI), in order to highlight the presence of any differences and correlations in these two different species of fish. Thirty adult fish of Mugil cephalus and thirty of Sparus aurata were used. From each fish 0.6 ml of blood was collected. TOS and TAC indicators were measured in serum obtained from samples previously clotted and centrifuged. Our results showed statistically significant differences between the two species in TAC. TOS and OSI did not show significant differences between Gilthead sea bream and Flathead mullet. A positive relationship between TOS and TAC was found in Flathead mullet (Mugil cephalus), and a negative relation between TOS and TAC in Gilthead sea bream (Sparus aurata). Our study indicates that the oxidative status and the relationship between total oxidant status (TOS) and total antioxidant capacity (TAC) in serum are probably dependent on the fish species and are affected by different feeding habits. Keywords: total oxidant status, total antioxidant capacity, teleost species, redox balance Journal: Veterinární medicína Pages: 691-695 Volume: 60 Issue: 12 Year: 2015 DOI: 10.17221/8583-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8583-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201512-0004.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:12:id:8583-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Tlak Gajger Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: J. Ribaric Author-Workplace-Name: Ministry of Agriculture, Zagreb, Croatia Author-Name: M. Matak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: L. Svecnjak Author-Workplace-Name: Faculty of Agriculture, University of Zagreb, Zagreb, Croatia Author-Name: Z. Kozaric Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: S. Nejedli Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: I.M. Smodis Skerl Author-Workplace-Name: Agricultural Institute of Slovenia, Ljubljana, Slovenia Title: Zeolite clinoptilolite as a dietary supplement and remedy for honeybee (Apis mellifera L.) colonies Abstract: Control of the nosema disease poses a major challenge, and therefore, treatment of this serious parasitic disease using natural preparations could be of great benefit. The aim of this study was to test the performance of zeolite clinoptilolite as a curative measure against honeybee colonies (Apis mellifera L.) naturally infected by Nosema ceranae. The histopathological structure, and the content and distribution of mucosubstances and histochemical activity of aminopeptidase and non-specific esterase in the midgut mucosa of honeybees originating from colonies fed sugar syrup supplemented with zeolite minerals was studied. A decline in the number of spores in honeybees fed with zeolite clinoliptolite was observed on the first sampling day (Day 10; 53.25 ± 15.15 million spores/bee), though a statistically lower number of spores in comparison to the control was confirmed on Day 20 (41.08 ± 9.4 million spores/bee), Day 30 (28.42 ± 7.79 million spores/bee) and Day 40 (24 ± 6.25 million spores/bee). The possibility of using natural zeolites as a dietary supplement for honeybee colonies as a preventative measure and for the reduction of the deleterious effects of nosemosis is discussed. Keywords: Apis mellifera, Nosema ceranae, zeolite clinoptilolite, dietary supplement Journal: Veterinární medicína Pages: 696-705 Volume: 60 Issue: 12 Year: 2015 DOI: 10.17221/8584-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8584-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201512-0005.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:12:id:8584-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Dokic Author-Workplace-Name: Small Animal Referral Centre Sibra, Bratislava, Slovak Republic Author-Name: W. Pirog Author-Workplace-Name: Small Animal Referral Clinic Jaggy, Prague, Czech Republic Title: Emphysematous lesions in the right cranial lung lobe and torsion of the right medial lung lobe in a British shorthair cat: a case report Abstract: A three-year-old male British shorthair cat that had exhibited progressive lethargy and intermittent dyspnoea for 14 days was referred for evaluation of acute respiratory deterioration. Clinical findings included rapid and shallow breathing, pale mucous membranes, sound suppression on the right side, and a subcutaneous haematoma in the right epigastric area. Serum biochemistry analysis showed leukocytosis and thrombocytosis. Radiographs revealed hydropneumothorax, a broken eighth right rib, atelectatic right cranial lung lobe (RCrL), and consolidation of the right middle lobe (RML). Doppler examination revealed sonographic changes in the echotexture of both lobes and venous flow was absent in the twisted RML. Furthermore, bronchoscopy showed proximal narrowing of the cat's RML bronchus. Exploratory surgery via medial sternotomy confirmed torsion of the RML and identified deteriorated gas-containing lesions in the collapsed RCrL. Both lung lobes were removed by standard lobectomy, and postoperative recovery was without major complications. Histopathological examination diagnosed multiple bullae and blebs, with significant subpleural haemorrhages in the atelectatic RCrL, whereas tissue congestion with haemorrhages, necrosis, and thrombosis typical for lung lobe torsion were observed in the RML. No other underlying aetiology was apparent. Two months post-operatively, the cat presented with similar acute onset of dyspnoea and spontaneous pneumothorax and was euthanised at the owner's request. The autopsy revealed identical new emphysematous changes in the contra-lateral lung lobes that had been absent at the time of surgery. Emphysematous lesions, regardless of their origin, should be considered in the etiopathology of lung lobe torsion. Keywords: bullae, lobular emphysema, spontaneous pneumothorax, twisted lobe, lobectomy Journal: Veterinární medicína Pages: 706-711 Volume: 60 Issue: 12 Year: 2015 DOI: 10.17221/8586-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8586-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201512-0006.txt Handle: RePEc:caa:jnlvet:v:60:y:2015:i:12:id:8586-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: VOLUME 60, CONTENTS; AUTHOR INDEX; AUTHOR INSTITUTION INDEX; LIST OF REVIEWERS Journal: Veterinární medicína Pages: X1 Volume: 60 Issue: 12 Year: 2015 DOI: 10.17221/8623-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8623-VETMED.html File-Format: text/html Handle: RePEc:caa:jnlvet:v:60:y:2015:i:12:id:8623-VETMED