Template-Type: ReDIF-Article 1.0 Author-Name: M. Klimczuk Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: P. Podlasz Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: W. Sienkiewicz Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Franke-Radowiecka Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Dudek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: Z. Pidsudko Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: M. Chmielewska-Krzesinska Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: J. Kaleczyc Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Title: Immunohistochemical characterisation of neurons in the mandibular ganglion and nerve fibres supplying the porcine mandibular gland Abstract: The present study was designed to investigate the chemical coding of neurons in the mandibular ganglion (MGn) and nerve fibres supplying the porcine mandibular gland (MGl) with the use of immunofluorescence and RT-PCR. The cryostat sections from MGn and MGl were processed for double-labelling immunohistochemistry using antisera against vesicular acetylcholine transporter (VAChT), choline acetyltransferase (ChAT), dopamine β-hydroxylase (DβH), neuronal nitric oxide synthase (nNOS), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), galanin (GAL), substance P (SP) and calcitonin gene-related peptide (CGRP). The MGl was found to be richly supplied by VAChT-positive nerve fibres that surrounded intra- and interlobular salivary ducts. A large number of VAChT-immunoreactive (VAChT-IR) nerve terminals were also observed around acini. Many periductal and periacinar nerve fibres stained positive for DβH. Immunoreactivity to GAL, NPY or VIP was observed in an intermediate number of nerve terminals which were associated with both salivary ducts and acini. Double-immunostaining revealed that in MGn nearly all neurons stained positive for VAChT/ChAT (98.45 ± 0.59%, mean ± SEM) and nNOS (99.71 ± 0.18%). An intermediate number of the nerve cell bodies displayed immunoreactivity to NPY or VIP (18.67 ± 0.52% and 8.11 ± 0.36%, respectively). Single GAL-IR and CGRP-positive neurons were also observed. RT-PCR revealed the presence of transcripts of ChAT, VAChT, nNOS, NPY, VIP and GAL. For SP and DβH very weak signals were observed. RT-PCR with primers targeting CGRP did not generate any PCR product. Keywords: mandibular gland, mandibular ganglion, neuropeptides, immunohistochemistry, RT-PCR, swine Journal: Veterinární medicína Pages: 361-373 Volume: 61 Issue: 7 Year: 2016 DOI: 10.17221/221/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/221/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201607-0001.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:7:id:221-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E.J. Hong Author-Workplace-Name: College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea Author-Name: C. Sim Author-Workplace-Name: Department of Biology, Baylor University, Waco, Texas, USA Author-Name: J.S. Chae Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, Research Institute and BK21 Program for Veterinary Science, Seoul, Republic of Korea Author-Name: H.C. Kim Author-Workplace-Name: College of Veterinary Medicine, Gangwon National University, Gangwon, Republic of Korea Author-Name: J. Park Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Chonbuk, Republic of Korea Author-Name: K.S. Choi Author-Workplace-Name: College of Environmental Science, Kyungbook National University, Kyungbook, Republic of Korea Author-Name: D.H. Yu Author-Workplace-Name: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Author-Name: C.H. Park Author-Workplace-Name: Veterinary Service Laboratory, South branch of Gangwondo, Republic of Korea Author-Name: J.G. Yoo Author-Workplace-Name: Laboratory of Veterinary Clinics, National Institute of Animal Science Rural Development Administration, Republic of Korea Author-Name: B.K. Park Author-Workplace-Name: College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea Title: Ultrastructural and molecular identification of Sarcocystis tenella (Protozoa, Apicomplexa) in naturally infected Korean native goats Abstract: Sheep is the intermediate host of the protozoan parasite Sarcocystis tenella, while the dog is its definitive host. This study was conducted to determine the prevalence of natural infection with S. tenella in slaughtered Korean native goat, Capra hircus coreanae, in the Republic of Korea. H-E stained heart tissues were investigated for the presence of sarcocysts. Of the 103 goats, three (2.91%) were diagnosed as positive for S. tenella by light, electron microscopic and molecular examination. The histopathological study showed a low frequency of microscopic Sarcocystis infection in slaughtered goats. In transmission electron microscopy, the sarcocysts were confirmed as S. tenella. Further DNA sequencing and phylogenic analysis support our identification of S. tenella with a 18S rRNA sequence identity of 100% between the experimental sequence and S. tenella. To our knowledge, this is the first record of S. tenella in Korean native goats from Korea. We thus report that the domestic goat is another intermediate host for S. tenella. Keywords: Capra hircus coreanae, cardiac muscle, Sarcocystis tenella, sarcocysts, bradyzoites, ultrastructure, molecular biology Journal: Veterinární medicína Pages: 374-381 Volume: 61 Issue: 7 Year: 2016 DOI: 10.17221/93/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/93/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201607-0002.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:7:id:93-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R.M. Medeiros Author-Workplace-Name: Sao Paulo State University, Jaboticabal Campus, Jaboticabal, Sao Paulo, Brazil Author-Name: M.A.M. Silva Author-Workplace-Name: College of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo, Rio Grande do Sul, Brazil Author-Name: P.P.M. Teixeira Author-Workplace-Name: Veterinary Hospital, Para Federal University, Castanhal, Para, Brazil Author-Name: L.G.G.G. Dias Author-Workplace-Name: Sao Paulo State University, Jaboticabal Campus, Jaboticabal, Sao Paulo, Brazil Author-Name: D.G. Chung Author-Workplace-Name: Sao Paulo State University, Jaboticabal Campus, Jaboticabal, Sao Paulo, Brazil Author-Name: C.C. Zani Author-Workplace-Name: Sao Paulo State University, Jaboticabal Campus, Jaboticabal, Sao Paulo, Brazil Author-Name: M.A.R. Feliciano Author-Workplace-Name: Sao Paulo State University, Jaboticabal Campus, Jaboticabal, Sao Paulo, Brazil Author-Name: M.E.B.A.M. Da Conceicao Author-Workplace-Name: Sao Paulo State University, Jaboticabal Campus, Jaboticabal, Sao Paulo, Brazil Author-Name: M.R.F. Machado Author-Workplace-Name: Sao Paulo State University, Jaboticabal Campus, Jaboticabal, Sao Paulo, Brazil Author-Name: A.G. Rocha Author-Workplace-Name: Sao Paulo State University, Jaboticabal Campus, Jaboticabal, Sao Paulo, Brazil Author-Name: G.O. Chierice Author-Workplace-Name: Sao Carlos Chemistry Institute, Sao Paulo University, Sao Paulo, Brazil Author-Name: L.N. Coutinho Author-Workplace-Name: Animal Health and Production Institute, Amazonia Rural Federal University, Belem, Para, Brazil Author-Name: J.G. Padilha Filho Author-Workplace-Name: Sao Paulo State University, Jaboticabal Campus, Jaboticabal, Sao Paulo, Brazil Title: Use of castor bean polymer in developing a new technique for tibial tuberosity advancement for cranial cruciate ligament rupture correction in dogs Abstract: The purpose of the current study was to develop a new tibial tuberosity advancement (TTA) technique, by replacing the original titanium cage with a Ricinus communis polyurethane resin-made wedge polymer. The implants were manufactured using the same size and angles of the original titanium cages, though larger distally. The modified TTA technique (TTAm) was performed in 42 knees of 35 dogs diagnosed with rupture of the cranial cruciate ligament (RCCL). Animals were submitted to radiographic and gait assessments preoperatively, early postoperatively and following 30, 60, 90 and 120 days. All animals exhibited good clinical outcome. There were no cases of impaired healing or bone resorption until 120 days postoperatively and there was no patient with patellar distress after TTAm. Scores of gait evaluation revealed differences between time points. There were also differences between the evaluations of control and pre- and post-operative times. However, there was no difference among the assessment of 30 days and the following time points. The use of the polyurethane polymer for TTAm was advantageous, not only due to biocompatibility and osseointegration, but also for providing easy handling; it can be moulded intra-operatively if necessary. Moreover, it allows precise adaptation to the osteotomy site, as opposed to the original TTA metallic implants, which cannot be moulded. It is suggested that incision lengths for TTAm are slightly shorter than those required for the conventional TTA as this requires the distal fixation of the plate at the beginning of the middle third of the body of the tibia. The TTAm does not require the use of fixation plates and it is performed only at the cranial aspect of the tibia. The method of attachment of the tibial tuberosity in the craniocaudal direction was effective. The setting associated with the use of the polyurethane polymer allowed simplification of the technique for easier implementation, and the amount of implant material required to perform TTAm was reduced in comparison to the conventional TTA. This technique can be used for treating the knees of dogs with RCCL, and provides for easy execution, less invasiveness to the tissues of the knee joint and more versatility in comparison to conventional TTA. Keywords: orthopaedic implant, corrective osteotomy, knee, ligament tear, canine Journal: Veterinární medicína Pages: 382-388 Volume: 61 Issue: 7 Year: 2016 DOI: 10.17221/168/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/168/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201607-0003.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:7:id:168-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Pugliese Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: A. La Pietra Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: L. Liotta Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: F. Macrì Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: A. Palumbo Piccionello Author-Workplace-Name: School of Veterinary Medical Sciences, University of Camerino, Matelica, Macerata, Italy Author-Name: M. De Majo Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Title: Ultrasonographic measurements of abdominal lymph nodes in growing puppies Abstract: The sonographic appearance of the normal abdominal lymph nodes in adult dogs has been well described, but the data in puppies are scarce and of poor quality. The aim of the current study was to evaluate any differences in abdominal lymph node sonographic measurements in puppies of various sizes and to determine whether any differences were correlated with growth and weight gain during the first 10 weeks of life. By an approach based on prospective and serial measurements, length, width and thickness of jejunal, medial iliac and hypogastric nodes were obtained in twenty-one healthy puppies of various sizes, at six (T0), eight (T1) and ten (T2) weeks of age. The relationship between body weight and length, width and thickness of lymph nodes was evaluated using a Pearson correlation analysis. An ANOVA test was used to compare the measurements at different ages. Jejunal and iliac lymph nodes were the largest in large breed dogs. In large-sized puppies only the length of the jejunal lymph nodes correlated positively with width and body weight. Length of medial iliac lymph nodes correlated positively with width and body weight in all three sizes. None of the measurements of hypogastric lymph nodes were related to body weight. In large-sized puppies jejunal and iliac lymph nodes increased in length and width with age; in medium-sized puppies only iliac lymph nodes increased; in small-sized puppies jejunal and iliac lymph nodes significantly decreased in length and thickness. In conclusion, the lymph node sizes in young animals are directly related to body weight and do not decrease with growth during the first 10 weeks of life, except in small-sized puppies. Keywords: abdominal lymph nodes, puppies, size, measurements, ultrasonography Journal: Veterinární medicína Pages: 389-393 Volume: 61 Issue: 7 Year: 2016 DOI: 10.17221/216/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/216/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201607-0004.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:7:id:216-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Spadola Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: G. Barillaro Author-Workplace-Name: Clinica Veterinaria, San Giorgio, Reggio Calabria, Italy Author-Name: M. Morici Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: A. Nocera Author-Workplace-Name: Clinica Veterinaria, San Lorenzo, Palermo, Italy Author-Name: Z. Knotek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Title: The practical use of computed tomography in evaluation of shell lesions in six loggerhead turtles (Caretta caretta) Abstract: Six adult loggerhead turtles were found stranded and were rescued near Sicily within a period of 12 months. Macroscopically apparent lesions of the shell were present. After thorough physical examination, ketamine-dexmedetomidine-atipamezole induction and tracheal tube insertion all six patients underwent computed tomographic examination under inhalant anaesthesia with isoflurane. A vertebral lesion at the level of the 3rd thoracic-lumbar vertebra with vertebral lamina and the vertebral body being involved without compression of the spinal cord, a vertebral lesion at the level of the 7th thoracic-lumbar vertebra and a vertebral lesion at the level of the 8th thoracic-lumbar vertebra were recorded in the first female. Loss of the shell near the left carapace-plastron bridge, with massive haemorrhage and compression of organs were present in the second female. The remaining four turtles had only superficial lesions with no involvement of bones and organs of the coelom. Computed tomography was proved to be a valuable non-invasive method for clinical examination of stranded sea turtles. Keywords: chelonians, shell fracture, imaging methods, computed tomography Journal: Veterinární medicína Pages: 394-398 Volume: 61 Issue: 7 Year: 2016 DOI: 10.17221/68/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/68/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201607-0005.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:7:id:68-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P.T.C. Guimaraes-Okamoto Author-Workplace-Name: School of Veterinary Medicine and Animal Science, University Estadual Paulista, Botucatu, Sao Paulo, Brazil Author-Name: S.S. Geraldes Author-Workplace-Name: School of Veterinary Medicine and Animal Science, University Estadual Paulista, Botucatu, Sao Paulo, Brazil Author-Name: J.F.A. Ribeiro Author-Workplace-Name: School of Veterinary Medicine and Animal Science, University Estadual Paulista, Botucatu, Sao Paulo, Brazil Author-Name: A.N.L.S. Vieira Author-Workplace-Name: School of Veterinary Medicine and Animal Science, University Estadual Paulista, Botucatu, Sao Paulo, Brazil Author-Name: L.P. Porto Author-Workplace-Name: School of Veterinary Medicine and Animal Science, University Estadual Paulista, Botucatu, Sao Paulo, Brazil Author-Name: P. Barretti Author-Workplace-Name: School of Medicine, University Estadual Paulista, Botucatu, Sao Paulo, Brazil Author-Name: M.L.G. Lourenco Author-Workplace-Name: School of Veterinary Medicine and Animal Science, University Estadual Paulista, Botucatu, Sao Paulo, Brazil Author-Name: A. Melchert Author-Workplace-Name: School of Veterinary Medicine and Animal Science, University Estadual Paulista, Botucatu, Sao Paulo, Brazil Title: Reversal of acute kidney injury after peritoneal dialysis in a dog: a case report Abstract: Acute kidney injury is characterised by a sudden injury to the renal parenchyma and causes defects in its excretory, metabolic and endocrine function. Dialysis therapy has been instituted in small animal clinics with the aim of removing the metabolic waste and correcting the electrolyte disturbances stemming from renal dysfunction. Peritoneal dialysis is a therapy based on the use of the peritoneum as a semipermeable membrane through which solutes and fluids are exchanged between blood from the peritoneal capillaries and the dialysis solution. This report describes a case of acute kidney injury stemming from drug therapy in a 13-year-old female mongrel canine. The patient exhibited anorexia, emesis, prostration and anuria and had a history of prolonged treatment with meloxicam. The patient also presented with azotaemia and metabolic acidosis. When anuria continued to persist after drug therapy (volume restoration, chemical and osmotic diuresis and renal vasodilation), peritoneal dialysis was instituted. Three cycles of peritoneal dialysis were performed; during the second cycle, the patient's anuria was reversed, and at the end of the third cycle she showed a significant reduction in azotaemia, hyperkalaemia and an improved metabolic acidosis. Therefore, peritoneal dialysis showed satisfactory clinical results and reversed anuria, reduced azotaemia and electrolyte disturbances, thereby providing clinical improvement. Keywords: dialysis therapy, small animals, renal dysfunction, acute kidney injury, blood gases, electrolytes, anuria Journal: Veterinární medicína Pages: 399-403 Volume: 61 Issue: 7 Year: 2016 DOI: 10.17221/13/2016/VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/13/2016/VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201607-0006.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:7:id:13-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: T.S. Hwang Author-Workplace-Name: College of Veterinary Medicine, Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: Y.M. Yoon Author-Workplace-Name: College of Veterinary Medicine, Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: S.A. Noh Author-Workplace-Name: College of Veterinary Medicine, Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: D.I. Jung Author-Workplace-Name: College of Veterinary Medicine, Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: S.C. Yeon Author-Workplace-Name: College of Veterinary Medicine, Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: H.C. Lee Author-Workplace-Name: College of Veterinary Medicine, Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea Title: Pneumatosis coli in a dog - a serial radiographic study: a case report Abstract: A 12-year-old intact female poodle was presented with a history of an acute episode of tenesmus and passage of ribbon-shaped stools. Anaemia, leucocytosis, hypoalbuminaemia, hyperglycaemia, and elevated ALP were found. Faecal floatation and wet mount preparation were negative for parasites. Anaerobic faecal culture resulted in a heavy growth of Clostridium. Survey abdominal radiographs revealed extensive intramural emphysema of colon and rectum. Ultrasonography of the abdomen revealed bright echoes within the layers of the colon wall, confirming the accumulation of intramural gas. Abdominal computed tomography revealed extraluminal gas tracking along the colon and the rectum. Based on the radiographic, ultrasonographic, and computed tomographic findings, the present case was diagnosed as pneumatosis coli with an underlying cause of bacterial overgrowth. The patient was treated with antibiotics for seventeen days. Clinical signs were resolved after three days of treatment. Decreased intramural gas accumulation was evident during radiography of the abdomen performed at fourteen days after the initial evaluation. Therefore, pneumatosis coli should be considered when a dog is presented with clinical signs of colitis. Keywords: colon, computed tomography, dog, emphysema Journal: Veterinární medicína Pages: 404-408 Volume: 61 Issue: 7 Year: 2016 DOI: 10.17221/255/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/255/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201607-0007.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:7:id:255-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Yaygingul Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Author-Name: B. Kibar Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Author-Name: I. Suner Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Author-Name: A. Belge Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Title: Pectus excavatum in a cat: a case report Abstract: This case report aims to clinically and radiologically describe a case of feline pectus excavatum and to evaluate the treatment results. An 8-month-old female cat weighing 3.2 kg was presented to the Surgery Clinic, Faculty of Veterinary Medicine, Adnan Menderes University with respiratory distress. Following radiographic and clinical examinations, the condition was diagnosed as pectus excavatum. Surgical correction was performed using a U-shaped external splint. Post-operative thoracic radiography showed that the concavity of the sternum was reduced. The splint was removed five weeks post-surgery. Two months after surgery, the cat was clinically normal. Keywords: cat, pectus excavatum, external splint, surgical correction Journal: Veterinární medicína Pages: 409-411 Volume: 61 Issue: 7 Year: 2016 DOI: 10.17221/285/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/285/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201607-0008.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:7:id:285-2015-VETMED