Template-Type: ReDIF-Article 1.0 Author-Name: M. Freick Author-Workplace-Name: Veterinary Practice Zettlitz, Zettlitz OT Methau, Germany Author-Name: J. Weber Author-Workplace-Name: Veterinary Practice Zettlitz, Zettlitz OT Methau, Germany Author-Name: O. Passarge Author-Workplace-Name: Veterinary Practice Zettlitz, Zettlitz OT Methau, Germany Author-Name: S.D. Kevork Author-Workplace-Name: Statistical Consulting Unit StaBLaB, Munich, Germany Title: Effects of erythromycin on milk yield, rumen contraction rate and blood beta-hydroxybutyrate concentration in dairy cows after laparoscopic reposition of the left displaced abomasum Abstract: The objective of this study was to evaluate the beneficial effects of erythromycin (Emy), a prokinetic drug active at the level of the abomasum, duodenum and proximal duodenum, in dairy cows undergoing a laparoscopic repositioning of left abomasal displacement (LAD). A total of 71 German Holstein cows were randomly assigned into three groups. Cows of the group Emy1 received erythromycin once preoperatively (10 mg/kg; intramuscularly). Animals of the group Emy3 were additionally treated twice after surgery at intervals of 24 h each (10 mg/kg; intramuscularly), while cows in the control group did not receive erythromycin. The milk yield of the patients was documented on Days 0, 1, 2, 3, 6 and 10 and the rumen contraction rate (RCR) was determined on Days 1 and 3. Additionally, the measurement of the concentration of beta-hydroxybutyrate (BHB) in whole blood samples was performed on Days 0, 1 and 3. Significant differences between the study groups regarding the cure rates, milk yield (in relation to Day 0); BHB concentrations and RCR could not be determined on the observation dates. With respect to a prudent use of antibiotics in livestock, the application of erythromycin as a prokinetic drug cannot be recommended for the present in cows undergoing a laparoscopic correction of LAD. Keywords: cattle, dislocatio abomasi, endoscopy, prokinetic drugs, gastrointestinal mobility Journal: Veterinární medicína Pages: 413-420 Volume: 61 Issue: 8 Year: 2016 DOI: 10.17221/20/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/20/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201608-0001.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:8:id:20-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Ozkaraca Author-Workplace-Name: Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey Author-Name: S. Ceribasi Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: A.O. Ceribasi Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: A. Kilic Author-Workplace-Name: Sivrice Vocational High School, Firat University, Elazig, Turkey Author-Name: S. Altun Author-Workplace-Name: Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey Author-Name: S. Comakli Author-Workplace-Name: Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey Author-Name: H. Ongor Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Title: Determination of Coxiella burnetii in bovine foetuses using PCR and immunohistochemistry Abstract: This study was aimed at detection of Coxiella burnetii in bovine foetuses using polymerase chain reaction (PCR) and immunohistochemistry (IHC) and at an estimation of its frequency in Eastern Turkey. Stamp, Giemsa, and Gimenez stains were used in addition to PCR and IHC to determine the presence of C. burnetii in samples from 70 bovine foetuses. While the staining methods did not detect the agent by direct visualisation of C. burnetii on smears, PCR and IHC identified its presence in two of the foetuses. The distribution of antigens in these two foetuses was, in decreasing order of concentration, in the spleen, the thymus, the lungs, the liver, and the kidneys. We conclude that C. burnetii diagnosis in bovine foetuses can be reliably performed using PCR and IHC. In addition, the frequency of 1.42% of C. burnetii positivity in bovine foetuses reported here was the first time that the presence of this agent was determined in Eastern Turkey. Keywords: Q fever, diagnostic method, IHC, cow, Eastern Turkey Journal: Veterinární medicína Pages: 421-427 Volume: 61 Issue: 8 Year: 2016 DOI: 10.17221/138/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/138/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201608-0002.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:8:id:138-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Vaclavkova Author-Workplace-Name: Institute of Animal Science, Prague, Czech Republic Author-Name: Z. Volek Author-Workplace-Name: Institute of Animal Science, Prague, Czech Republic Author-Name: J. Belkova Author-Workplace-Name: Institute of Animal Science, Prague, Czech Republic Author-Name: D. Duskova Author-Workplace-Name: Institute of Animal Science, Prague, Czech Republic Author-Name: M. Czauderna Author-Workplace-Name: The Kielanowski Institute of Animal Physiology and Nutrition, Jablonna, Poland Author-Name: M. Marounek Author-Workplace-Name: Institute of Animal Science, Prague, Czech Republic Title: Effect of linseed and the combination of conjugated linoleic acid and linseed on the quality and oxidative stability of pig meat and subcutaneous fat Abstract: The aim of this experiment was to test the hypothesis that conjugated linoleic acid (CLA) in diets of finishing pigs fed linseed can improve the quality and oxidative stability of meat and subcutaneous fat. Twenty-four Prestice Black-Pied pigs (barrows and gilts) were divided into three groups and were fed a basal diet and diets supplemented with ground linseed (70 g/kg), or linseed combined with conjugated linoleic acid (20 g CLA-oil/kg). The trial duration was 53 days. Measurements included slaughter and meat quality parameters, oxidative stability determination, and fatty acid profile of meat and subcutaneous fat. The experimental data were analysed using one-way analysis of variance. Neither linseed nor linseed with CLA significantly influenced weight gain, lean percentage, muscle depth, backfat thickness, drip loss, meat shear force, dry matter, intramuscular fat or cholesterol (P > 0.05). Dietary supplementation with linseed increased the percentage of linolenic acid in the fatty acids of meat and backfat and resulted in higher production of aldehydes. Dietary CLA did not influence the susceptibility of lipids to oxidation. Supplementation with CLA significantly increased CLA proportions in fatty acids of meat and backfat, reduced proportions of monounsaturated fatty acids, and increased proportions of saturated fatty acids in backfat (P < 0.05). The concentration of CLA (in mg/100 g of fresh tissue) in backfat was almost fifty times higher than in meat. Both meat and backfat of pigs fed CLA-free diets contained CLA, probably as a result of microbial conversion of linoleic acid in the intestine. It can be concluded that CLA changed the fatty acid profile of meat and backfat, but did not improve oxidative stability and other meat quality traits of pigs fed linseed. Keywords: CLA, meat quality, backfat, TBARS, fatty acids Journal: Veterinární medicína Pages: 428-435 Volume: 61 Issue: 8 Year: 2016 DOI: 10.17221/117/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/117/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201608-0003.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:8:id:117-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.M. Hashemiasl Author-Workplace-Name: Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Author-Name: S. Azizi Author-Workplace-Name: Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Author-Name: D. Torkamani Author-Workplace-Name: Private Section Practitioner, Urmia, Iran Title: Radiographic findings in sheep with abomasal phytobezoariasis Abstract: The purpose of this study was to characterise the radiographic appearance of abomasal phytobezoars in sheep as well as to evaluate the utility of abdominal radiography to identify them. Twenty-seven fat-tailed Herrick sheep with a clinical suspicion of abomasal impaction were examined radiographically. Abdominal survey radiographs in right lateral recumbency were taken. Abomasal phytobezoars (AP) were seen in abdominal survey radiographs in 25/27 sheep (92%). Their radiographic survey appearance was round-to-oval masses with radiopaque margins and radiopacity similar to the ingesta centrally. An additional gastrographic barium study was performed in six of the sheep, followed by exploratory laparotomy where phytobezoars were removed through abomasotomy. The optimal time to visualise the APs was 48 h post-contrast. A significant correlation was noted between phytobezoars size in radiology and surgery (r = 0.651, P < 0.001). Use of the barium study can improve the phytobezoar-ingesta contrast and visibility of the phytobezoars. Plain radiography with sheep positioned in right lateral recumbency is a useful supplementary technique which can be used to evaluate abomasal phytobezoariasis. This study shows that radiography is a suitable diagnostic method for detecting the presence of, but not the number of, abomasal phytobezoars in sheep. Keywords: phytobezoar, abomasography, radiography, barium, abomasum, abomasotomy Journal: Veterinární medicína Pages: 436-442 Volume: 61 Issue: 8 Year: 2016 DOI: 10.17221/9/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/9/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201608-0004.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:8:id:9-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D.A. Bala Author-Workplace-Name: Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: E. Eraslan Author-Workplace-Name: Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: I. Akyazi Author-Workplace-Name: Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: E.E. Ekiz Author-Workplace-Name: Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: M. Ozcan Author-Workplace-Name: Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: U. Cotelioglu Author-Workplace-Name: Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: M. Arslan Author-Workplace-Name: Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Title: Freezing and storage of leukodepleted erythrocyte suspensions Abstract: Studies on the frozen storage of human blood products have benefited veterinary transfusion medicine in recent years, but the long-term cryopreservation of canine red blood cells (RBCs) has not yet been thoroughly investigated. Further, no studies are available with respect to the frozen storage of leukocyte-depleted canine red blood cells (LD-RBCs). The objective of the current study was to investigate time-dependent effects of long-term frozen storage on leukocyte-depleted canine RBCs. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged for 5 min at 22 °C and 4200 × g in a cryogenic microcentrifuge and concentrated RBC (pRBC) suspensions were obtained. Leukocyte depletion was achieved by filtration (2.6 log10). Then, the filtrated samples were prewashed three times in 0.9% NaCl solution and were allocated into three subgroups to be evaluated at three different time points (Day 0, Month 4 and Month 6). The samples for cryopreservation were subjected to glycerolisation and then stored at -80 °C for 4- and 6-month periods. At the end of this period pRBC units were thawed by manual agitation in a water bath maintained at 36-38 °C, centrifuged and then washed in a consecutive series of 12%, 1.6% and 0.9% of NaCl + 0.2 dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), supernatant haemoglobin (SupHb), sodium (Na+) and potassium (K+) levels, residual glycerol concentrations and haemograms of thawed and deglycerolised pRBC samples were evaluated together with those of Day 0. Sterility tests were performed on all samples for bacterial contamination. No statistically significant differences were noted except for Hct and SupHb levels. No bacterial contamination was noted in any of the samples on the basis of sterility tests. It was found that the described glycerolisation procedure could be a method of choice in the cryopreservation of leukocyte-depleted pRBCs (LD-pRBCs) since no negative effect was observed on the quality of the products and long-term frozen storage did not cause RBC destruction. Keywords: canine, cryopreservation, leukocyte, quality, red blood cell Journal: Veterinární medicína Pages: 443-448 Volume: 61 Issue: 8 Year: 2016 DOI: 10.17221/209/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/209/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201608-0005.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:8:id:209-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Pejchalova Author-Workplace-Name: Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic Author-Name: S. Zabcikova Author-Workplace-Name: Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic Author-Name: L. Silhova Author-Workplace-Name: Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic Author-Name: D. Silha Author-Workplace-Name: Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic Author-Name: I. Brozkova Author-Workplace-Name: Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic Author-Name: M. Haslova Author-Workplace-Name: Trnova Veterinary Clinic, Pardubice, Czech Republic Title: Presence of Arcobacter species in pet cats and dogs in the Czech Republic Abstract: This study was conducted to evaluate the occurrence of the genus Arcobacter in cats and dogs in the Czech Republic. These animals may be carriers of the bacteria and potential sources of human infection. Oral smears were collected from animals using smear swabs and brushes. Based on previous studies, commercially available DNA kits were used for DNA isolation. Samples were analysed using polymerase chain reaction (PCR) and evaluated using gel electrophoresis. Overall, 178 oral smears were tested, of which 108 were from dogs and 70 were from cats. Out of all smears, five were positive, of which four samples were from dogs and one from a cat. In all five positive cases, PCR confirmed the presence of Arcobacter butzleri. In follow-up sampling, the presence of Arcobacter butzleri was demonstrated in two samples from a dog. Keywords: Arcobacter butzleri, cat, dog, polymerase chain reaction, oral smears Journal: Veterinární medicína Pages: 449-455 Volume: 61 Issue: 8 Year: 2016 DOI: 10.17221/273/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/273/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201608-0006.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:8:id:273-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Suchy Author-Workplace-Name: Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: E. Strakova Author-Workplace-Name: Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: I. Herzig Author-Workplace-Name: Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Title: Levels of fatty acids in the whole body of hens and cocks of the Cobb 500 and Ross 308 hybrid combinations at the end of the fattening period Abstract: In this study, we determined the levels of individual fatty acids in the whole chicken body, as well as dry matter, nitrogenic substances and fat in Cobb 500 and Ross 308 chickens after 40 days of fattening. The highest level of all fatty acids (FA), regardless of the hybrid combination and sex of the chickens, was determined for oleic/elaidic acid, followed by palmitic, linoleic/linolelaidic, palmitoleic and stearic acids. In Cobb 500 cocks, higher values (P ≤ 0.05; P ≤ 0.01) were found for all saturated fatty acids (SaFA) compared to hens. With the exception of γ-linoleic acid, the levels of n-6 FA measured in cocks were higher (P ≤ 0.01) than in hens and n-3 FA showed higher levels of eicosatrienoic (P ≤ 0.05) and docosapentaenoic acids (P ≤ 0.01). Ross 308 hens had higher levels of most SaFA, with differences for palmitic (P ≤ 0.01), myristic, heptadecanoic and stearic acids (P ≤ 0.05). Regarding monounsaturated fatty acids (MUFA), hens contained higher levels of myristoleic (P ≤ 0.05) and oleic/elaidic acids (P ≤ 0.01). Cocks showed higher levels of n-6 FA except for linoleic/linolelaidic and γ-linolenic acids; higher levels were found for cis-8,11,14-eicosatrienoic, docosatetraenoic (P ≤ 0.05) and arachidonic acids (P ≤ 0.01). With the exception of α-linolenic acid, n-3 FA levels were higher in cocks, with differences in the levels of cis-5,8,11,14,17-eicosapentaenoic and docosapentaenoic acids (P ≤ 0.01). The results suggest possible directions for future research focused on the use of broiler chicken hybrids with more favourable proportions of n-6 FA and n-3 FA in fat and meat. Keywords: saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids, n-6, n-3 Journal: Veterinární medicína Pages: 456-463 Volume: 61 Issue: 8 Year: 2016 DOI: 10.17221/28/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/28/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201608-0007.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:8:id:28-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Salkova Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic Author-Name: M. Flajshans Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic Title: The first finding of Hassall's corpuscles in the thymi of cultured sturgeons Abstract: Histological examination of thymi from juvenile Acipenser ruthenus and Acipenser brevirostrum after haematoxylin and eosin staining revealed the presence of Hassall's corpuscles, in a scattered distribution within the thymic medulla in two of seven, and one of nine examined specimens, respectively. Morphologically, the Hassall's corpuscles were of the juvenile and premature type and no degenerative changes were seen. Keywords: Acipenser ruthenus, Acipenser brevirostrum, histology, thymus Journal: Veterinární medicína Pages: 464-466 Volume: 61 Issue: 8 Year: 2016 DOI: 10.17221/247/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/247/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201608-0008.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:8:id:247-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J.H. Kim Author-Workplace-Name: Konkuk University Veterinary Medical Teaching Hospital, Seoul, Republic of Korea Author-Name: H.J. Kim Author-Workplace-Name: Konkuk University Veterinary Medical Teaching Hospital, Seoul, Republic of Korea Author-Name: D.H. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: J.H. Yim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: S.J. Lee Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: K.H. Park Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: H.Y. Yoon Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Title: Successful response to imatinib in two dogs with inoperable grade III infiltrating mast cell tumours: a case report Abstract: Two dogs were presented owing to sudden rapid growth of cutaneous masses greater than 10 cm on the neck and axillary region, respectively. Based on history and results of physical examinations, blood work, fine needle aspiration, histopathological examination, and computed tomography, inoperable grade III infiltrating mast cell tumours were diagnosed. After the initiation of imatinib treatment, the masses markedly shrank and became undetectable within 10 days in both dogs, although none of the tumour specimens showed evidence of mutations in sequencing of c-kit exons 8 and 11. These results suggest that imatinib could be a therapeutic option in patients with surgically inoperable canine mast cell tumours, even those that are histopathologically high grade without c-kit exon 8 or 11 mutations. Keywords: canine, KIT, oncogene, target therapy, tyrosine kinase inhibitor Journal: Veterinární medicína Pages: 467-473 Volume: 61 Issue: 8 Year: 2016 DOI: 10.17221/87/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/87/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201608-0009.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:8:id:87-2016-VETMED