Template-Type: ReDIF-Article 1.0 Author-Name: C. Tothova Author-Workplace-Name: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: O. Nagy Author-Workplace-Name: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: G. Kovac Author-Workplace-Name: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Title: Serum proteins and their diagnostic utility in veterinary medicine: a review Abstract: The evaluation of serum proteins and their electrophoretic pattern is a well-established laboratory method in the diagnosis of many diseases in humans, which has replaced the biochemical determination of the concentrations of albumin and the ratio of albumin to globulins. The measurement of serum proteins may be an important diagnostic tool for the detection, diagnosis, and monitoring of various diseases and pathological processes. The results of serum protein electrophoresis can be one of the most useful diagnostic aids in a wide spectrum of diseases, including infectious and inflammatory diseases, renal or gastrointestinal disorders, immunodeficiency states, as well as paraproteinaemias caused by lymphoid or plasma cell neoplasia. Although many studies have been carried out to determine the usefulness of the determination of serum proteins and their electrophoretic pattern in various disease conditions and disorders also in animals, serum protein evaluation is still a relatively little-used diagnostic tool in veterinary medicine. In this article, methods of serum protein determination, their diagnostic utility in animal care practice and their different patterns in dysproteinaemias and paraproteinaemias are reviewed. Keywords: animals, serum proteins, electrophoresis, diagnosis, dysproteinaemia, paraproteinaemia Journal: Veterinární medicína Pages: 475-496 Volume: 61 Issue: 9 Year: 2016 DOI: 10.17221/19/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/19/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201609-0001.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:9:id:19-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Kaessmeyer Author-Workplace-Name: Department of Veterinary Medicine, Institute for Veterinary Anatomy, Freie Universitat Berlin, Berlin, Germany Author-Name: H. Huenigen Author-Workplace-Name: Department of Veterinary Medicine, Institute for Veterinary Anatomy, Freie Universitat Berlin, Berlin, Germany Author-Name: S. Al Masri Author-Workplace-Name: Department of Veterinary Medicine, Institute for Veterinary Anatomy, Freie Universitat Berlin, Berlin, Germany Author-Name: P. Dieckhoefer Author-Workplace-Name: Department of Veterinary Medicine, Institute for Veterinary Anatomy, Freie Universitat Berlin, Berlin, Germany Author-Name: K. Richardson Author-Workplace-Name: College of Veterinary Medicine, Murdoch University, Murdoch, Australia Author-Name: J. Plendl Author-Workplace-Name: Department of Veterinary Medicine, Institute for Veterinary Anatomy, Freie Universitat Berlin, Berlin, Germany Title: Corpus luteal angiogenesis in a high milk production dairy breed differs from that of cattle with lower milk production levels Abstract: Globally, high producing, young dairy cows often have health and fertility problems such as ovarian, uterine and placental dysfunction, mastitis and impaired wound healing. These health issues can result in a shortened average lifespan of fewer than three lactations. We hypothesise that many of these health issues may be an effect of altered angiogenesis. Hence, the aim of this pilot study was to investigate the status of vascularisation in the corpus luteum of a high milk-producing cattle breed (Holstein Friesian) compared with that of low milk producing beef cattle (Limousin) and of dual purpose cattle (Fleckvieh, Rotbunt). The corpus luteum was chosen because as a transient endocrine gland it is one of the few tissues with physiological angiogenesis in the adult. Blood vessels were labelled in paraffin sections of corpora lutea with the lectin Bandeiraea simplicifolia agglutinin I and the following angiogenesis parameters were analysed morphometrically using image analysis: (a) number of blood capillaries per mm2, (b) intercapillary distance, (c) percentage area occupied by blood vessels and (d) area of blood vessel lumina (μm2). This analysis revealed that the extent of corpus luteal vascularisation is greater in high milk-producing cattle than in beef and dual purpose cattle as shown by a significantly higher number of blood capillaries per mm2, significantly shorter intercapillary distances and a higher percentage of the corpus luteum area covered by blood vessels (non-significant). Only the average value of the luminal area of the luteal blood capillaries in the high milk-producing cattle is smaller than that measured in the low producing cattle. Keywords: Holstein Friesian, dairy cows, dual purpose cows, corpus luteum, vascularisation, capillary, angiogenesis Journal: Veterinární medicína Pages: 497-503 Volume: 61 Issue: 9 Year: 2016 DOI: 10.17221/72/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/72/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201609-0002.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:9:id:72-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Szabara Author-Workplace-Name: Faculty of Veterinary Science, Szent Istvan University, Budapest, Hungary Author-Name: J. Majer Author-Workplace-Name: Agricultural Corporation, Davod, Hungary Author-Name: L. Ozsvari Author-Workplace-Name: Faculty of Veterinary Science, Szent Istvan University, Budapest, Hungary Author-Name: C. Jakab Author-Workplace-Name: Faculty of Veterinary Science, Szent Istvan University, Budapest, Hungary Author-Name: W. Baumgartner Author-Workplace-Name: University of Veterinary Medicine, Vienna, Austria Title: Co-infection with bovine viral diarrhoea virus and Anaplasma marginale in a dairy cattle herd may lead to acute bovine anaplasmosis Abstract: This report describes an acute exacerbation of subclinical anaplasmosis manifesting itself in clinical signs in a large number of animals after infection with bovine viral diarrhoea virus (BVDV). The simultaneous transmission of BVDV and Anaplasma was unintended and most likely the result of a vaccination operation in a large Holstein-Friesian dairy cattle herd in Hungary. From Day 35 after the first vaccination, a total of 33 cows developed fever, depression, general weakness, lack of appetite, a sudden drop of milk production, anaemia, icterus, and tachypnoea on exercise. In addition, a total of seven abortions and three stillbirths occurred. Between Days 30 and 35 after the second vaccination four cows showed clinical signs typical of anaplasmosis, and two stillbirths occurred. The presence of Anaplasma marginale infection was demonstrated by haematological, biochemical, PCR and haemocytological examinations of blood samples collected from animals showing clinical signs as well as by necropsy. To assess the prevalence of infection in the herd, a specified number of animals belonging to different age groups were subjected to serological tests. The rate of seropositive animals was substantially higher (50%) in the older (3- to 4-year-old and more than 4-year-old) age groups than in the younger cows (10-30%). This study has demonstrated for the first time that if bovine animals susceptible to both A. marginale and BVDV are infected by the two pathogens roughly at the same time, the immunosuppressive effect of BVDV will support the progression of A. marginale infection and manifestation of the disease resulting in acute clinical signs. Keywords: BVDV, mixed infection, epidemiological investigation, immunosuppression, outbreak Journal: Veterinární medicína Pages: 504-515 Volume: 61 Issue: 9 Year: 2016 DOI: 10.17221/252/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/252/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201609-0003.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:9:id:252-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Matysiak Author-Workplace-Name: Poznan University of Life Sciences, Poznan, Poland Author-Name: P. Dudko Author-Workplace-Name: University of Life Sciences in Lublin, Lublin, Poland Author-Name: K. Dudek Author-Workplace-Name: Poznan University of Life Sciences, Poznan, Poland Author-Name: M. Dudek Author-Workplace-Name: Poznan University of Life Sciences, Poznan, Poland Author-Name: A. Junkuszew Author-Workplace-Name: University of Life Sciences in Lublin, Lublin, Poland Author-Name: P. Tryjanowski Author-Workplace-Name: Poznan University of Life Sciences, Poznan, Poland Title: The occurrence of pathogens in Rhipicephalus microplus ticks from cattle in Madagascar Abstract: Rhipicephalus microplus is one of the most important ectoparasites of cattle in tropical and subtropical regions. In ticks collected from cattle the pathogens Babesia bovis, Anaplasma spp. and Ehrlichia spp. can be detected. Here, we report the first detection of the pathogen Anaplasma phagocytophilum in Madagascar from ticks infesting cattle. Furthermore, we report for the first time Anaplasma ovis, Ehrlichia canis, Ehrlichia ewingii and Ehrlichia muris in both R. microplus and in Madagascar. We show no correlation between the detection of B. bovis, Ehrlichia spp. and Anaplasma spp. DNA within the same tick. Previous reports have demonstrated strong interactions between A. marginale, A. centrale and A. ovis in the same tick, as well between these pathogens and A. phagocytophilum. A strong correlation also existed between the occurrences of Ehrlichia species within the same tick. Our findings suggest that R. microplus ticks are potential vectors and reservoirs of many tick-borne diseases of cattle. Keywords: African ticks, Anaplasma, Babesia, Borrelia, Ehrlichia, zebu, Boophilus, southern cattle tick Journal: Veterinární medicína Pages: 516-523 Volume: 61 Issue: 9 Year: 2016 DOI: 10.17221/59/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/59/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201609-0004.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:9:id:59-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Holak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: Z. Lekston Author-Workplace-Name: Faculty of Computer Science and Materials Science, University of Silesia in Katowice, Chorzow, Poland Title: Shape memory compression anastomosis clips in gastrointestinal surgery in dogs Abstract: This paper describes clinical experiences with the use of shape memory nickel-titanium (NiTi) clips in gastrointestinal surgery in dogs. Side-to-side small bowel anastomosis was performed in eight dogs where intestinal continuity had to be restored after bowel resection. Billroth's operation I was performed in one case. Compression anastomosis clips with two-way shape memory were used in all surgical procedures. Intestinal and gastrointestinal anastomoses involving shape memory clips were effective in all patients. Anastomotic leaks were not observed, and all clips were expelled 5-7 days after surgery. The outcomes of surgical procedures performed on canine patients with the use of shape memory NiTi clips indicate that sutureless compression anastomosis is a safe, effective and simple method of restoring gastrointestinal continuity, which can be widely applied in veterinary practice. Keywords: CAC, intestinal anastomosis, NiTi, animals Journal: Veterinární medicína Pages: 524-527 Volume: 61 Issue: 9 Year: 2016 DOI: 10.17221/1/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201609-0005.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:9:id:1-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Uhrikova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: P. Scheer Author-Workplace-Name: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: J. Hlozkova Author-Workplace-Name: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: P. Suchy Author-Workplace-Name: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: M. Sepsi Author-Workplace-Name: Department of Internal Cardiology Medicine, University Hospital Brno, Brno, Czech Republic Title: Effects of acetylsalicylic acid on coagulation tests and haptoglobin concentrations in rabbits with permanent transvenous pacing Abstract: The aim of this study was to evaluate changes in coagulation tests, haptoglobin concentrations and leukocyte counts in rabbits with right-ventricle pacing medicated with acetylsalicylic acid (ASA). Blood was collected from 35 non-anaesthetised males from the jugular vein at baseline, one and two months after pacemaker implantation. Animals were divided into two groups: non-medicated and medicated with ASA. Total leukocyte and platelet counts were measured on an automatic veterinary flow cytometry haematological analyser. Prothrombin time, activated partial thromboplastin time, fibrinogen levels and D-dimers were determined from citrated blood. We found significantly elevated activated partial thromboplastin times and prothrombin times in ASA in comparison to the control group, but not within the ASA group over time. We also observed a decrease in platelet counts in the control group over time, but not in comparison to the ASA group. No significant changes in total leukocyte counts and haptoglobin concentrations were detected. Medication with ASA may alter coagulation profiles in rabbits with permanent transvenous pacing. Keywords: inflammation, D-dimers, pacemaker, Oryctolagus Journal: Veterinární medicína Pages: 528-532 Volume: 61 Issue: 9 Year: 2016 DOI: 10.17221/22/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/22/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201609-0006.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:9:id:22-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N. Timurkaan Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Author-Name: H. Eroksuz Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Author-Name: A. Cevik Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Author-Name: B. Karabulut Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Title: Cutaneous leiomyosarcoma with osteoid metaplasia in a budgerigar (Melopsittacus undulatus): a case report Abstract: Cutaneous leiomyosarcoma with osteoid metaplasia was diagnosed at the base of the right wing in a five-year-old female budgerigar. Grossly, the tumour mass was well circumscribed and solitary, and had a nodular appearance on section. On histological examination, the mass was composed of randomly arranged bundles of spindle-shaped cells that included mitotic, multi-nucleated and bizarre cells. In addition, within and between tumour areas, there were osteoid metaplasia foci. The bony trabeculae were usually localised in the areas around the haemorrhage, and exhibited focal mineralisation. Tumour cells were stained red using Masson's trichrome staining method. In addition, tumour cells were intensively positive for smooth muscle actin and focally positive for desmin and vimentin, but were negative for CD68 and S100 by immunohistochemical staining. The tumour reported here was defined as a cutaneous leiomyosarcoma with osteoid metaplasia by histopathological and immunohistochemical findings. Our findings may indicate that osteoid metaplasia should be considered in leiomyosarcoma in budgerigars. Keywords: desmin, CD68, smooth muscle actin, spindle-shaped cells, S100 Journal: Veterinární medicína Pages: 533-537 Volume: 61 Issue: 9 Year: 2016 DOI: 10.17221/43/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/43/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201609-0007.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:9:id:43-2016-VETMED