Template-Type: ReDIF-Article 1.0 Author-Name: E. Balikci Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Title: Antidermatophyte and antioxidant activities of Nigella sativa alone and in combination with enilconazole in treatment of dermatophytosis in cattle Abstract: The purpose of this study was to comparatively assess the antidermatophyte and antioxidant activities of enilconazole, Nigella sativa (NS) and enilconazole with NS in the treatment of dermatophytosis in cattle. A total of 24 cattle with clinically established diagnosis of dermatophytosis were used in the study. Trichophyton verrucosum was isolated and identified from all of the specimens stemming from the dermatophytosis-suspected animals. The lesion areas in Groups 1, 2 and 3 were treated as follows: enilconazole (three times at 3-day intervals), NS (once a day for two weeks) and enilconazole with NS, respectively. There were significant increases (P < 0.05) in plasma aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase levels and non-significant increases (P > 0.05) in creatinine and blood urea nitrogen levels after treatment in Group 1 when compared with Groups 2 and 3. After treatment, glutathione peroxidase, superoxide dismutase, glutathione levels increased (P < 0.05) and plasma thiobarbituric acid reactive substances levels decreased (P < 0.05) in Groups 1, 2 and 3 in comparison with before treatment. However, there were significant decreases (P < 0.05) in plasma thiobarbituric acid reactive substances levels and significant increases (P < 0.05) in glutathione peroxidase, superoxide dismutase, glutathione levels after treatment in Groups 2 and 3 when compared with Group 1. This study indicates that NS might have antidermatophyte and antioxidant effects in the treatment of dermatophytosis in cattle and the antidermatophyte effects of NS plus enilconazole was stronger among all groups. Keywords: Nigella sativa, enilconazole, antioxidants, trichophytosis, bovine Journal: Veterinární medicína Pages: 539-545 Volume: 61 Issue: 10 Year: 2016 DOI: 10.17221/32/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/32/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201610-0001.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:10:id:32-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Ciaputa Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: P. Bandoch Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: K. Lewandowska Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: J.A. Madej Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: M. Kandefer-Gola Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: I. Janus Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: M. Nowak Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Title: Immunohistochemical analysis of metastasising hepatocellular carcinomas in dogs Abstract: In this study, the immunohistochemical features of primary hepatocellular carcinomas and their metastases in visceral organs, including the lungs, spleen and kidneys were examined using antibodies against carcino-embryonic antigen (CEA) cytokeratin (CK) 7 and 20, CD4, CD8, minichromosome maintenance protein 3 (MCM3), vimentin, and alpha 1 foetoprotein (AFP). In addition, Mallory's connective tissue stain, van Gieson's stain and Gomori methenamine silver stain were used. The study was performed on liver samples collected post mortem from five mixed-breed dogs aged 9-12 years. The tumours were classified according to the World Health Organization Classification of Tumours. Strong expression of MCM3 and AFP was found in the hepatic cancer cells and in the metastases to the lungs, spleen and kidneys. The primary tumours and metastatic foci did not react positively with the anti-CD4, anti-CD8, CEA, CK7 and CK20 antibodies. The connective tissue in the primary tumour and the metastases showed a positive reaction to vimentin. Canine hepatocellular carcinomas that metastasise are highly-malignant well-differentiated tumours that produce AFP and trace amounts of both carcinoembryonic antigen and cytokeratin. Therefore, the metastasis resembles the primary tumour and has a common phenotype and genotype with the primary tumour. Keywords: cell markers, expression, CEA, CK7, CK20, CD4, CD8, vimentin, AFP, immunohistochemistry, pleomorphic cells, liver Journal: Veterinární medicína Pages: 546-552 Volume: 61 Issue: 10 Year: 2016 DOI: 10.17221/51/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/51/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201610-0002.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:10:id:51-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Kralova-Kovarikova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: L. Leva Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Knotek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: M. Toman Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Changes in lymphocyte function and subset counts in cats with spontaneous chronic kidney disease Abstract: Chronic kidney disease alters the immune response in humans and dogs and it is thought to be one of the causes of immunosuppression in cats. Haematological, biochemical, and immunological examinations were performed on blood samples obtained from 20 cats with chronic kidney disease, and were compared with the results of 18 healthy cats. In cats with chronic kidney disease, statistically significantly lower numbers of total lymphocytes (P < 0.01), especially T lymphocytes (P < 0.001), were observed. When the results were compared with the lymphocyte transformation test in healthy cats, decreased responses to phytohaemaglutinin, concanavalin A, and pokeweed mitogen were found in cats with chronic kidney disease. Our results document an alteration of counts and activity of lymphocytes in cats with chronic kidney disease. Thus, higher susceptibility to infection may be expected in these patients. Keywords: uraemia, immunosuppression, lymphopenia, mitogens, flow cytometry Journal: Veterinární medicína Pages: 553-559 Volume: 61 Issue: 10 Year: 2016 DOI: 10.17221/282/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/282/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201610-0003.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:10:id:282-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: C.C. Perez-Marin Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: E. Jimenez Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: E. Aguera Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Title: Choice of staining technique affects the morphological assessment of epididymal feline sperm Abstract: Morphological examination of spermatozoa remains a valuable tool for determining the quality of feline sperm for cryopreservation and/or for use in artificial insemination. Numerous staining techniques have been employed for feline sperm assessment, and several variations have also been reported. Forty-eight domestic cats were castrated and epididymal sperm samples were obtained. Sperm morphology was assessed using two staining methods, an air-dried stain (Giemsa-Wright) and a wet stain (eosin-nigrosin), to determine their relative abilities for estimation of the proportion of normal and abnormal spermatozoa. The effects of centrifugation and the cooling process on sperm morphology were also analysed. The mean percentage of morphologically normal spermatozoa was significantly lower in eosin-nigrosin-stained samples than in Giemsa-Wright-stained preparations (46.5% vs 53.4%; P < 0.01), both examined by conventional light microscopy. Results concerning head, midpiece and tail sperm abnormalities suggest that these methods differ in their capacity to identify sperm anomalies in cat. Although no significant differences were observed for secondary abnormalities, cytoplasmic droplets were significantly lower in Giemsa-Wright-stained samples (P < 0.01). Centrifugation or refrigeration of sperm samples did not affect the epididymal sperm morphology in evaluated samples. This study highlights the necessity of minimising morphological assessment variations in order to eliminate subsequent errors and to improve the reproducibility of sperm morphology assessment in different laboratories. Keywords: Felis catus, cat, spermatozoa, morphology, eosin-nigrosin stain, Giemsa-Wright stain Journal: Veterinární medicína Pages: 560-566 Volume: 61 Issue: 10 Year: 2016 DOI: 10.17221/132/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/132/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201610-0004.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:10:id:132-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Vokurka Author-Workplace-Name: Clinic of Dentistry, St. Anne's Faculty Hospital, Brno, Czech Republic Author-Name: E. Gopfert Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Blahutkova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: E. Buchalova Author-Workplace-Name: Clinic of Dentistry, St. Anne's Faculty Hospital, Brno, Czech Republic Author-Name: M. Faldyna Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Concentrations of growth factors in platelet-rich plasma and platelet-rich fibrin in a rabbit model Abstract: Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are platelet concentrates which have been used to improve healing and regeneration of damaged tissues. The concentration of growth factors greatly influences the final outcome of the treatment. For studying the effects of PRP and PRF, an animal model is required. The New Zealand White rabbit appears to be a good candidate for studying the features of these blood derivatives. This article presents for the first time the various preparation methods of blood derivatives with regard to concentration of growth factors - insulin-like growth factor, transforming growth factor and vascular endothelial growth factor. There was no statistically significant difference among the methods in the case of the first two factors. In the case of vascular endothelial growth factor, the lowest amount was detected in PRF. This study, even though performed on a limited number of animals, reports novel characterisation of the rabbit as an animal model for in vivo studies of the action of these blood derivatives. Keywords: blood derivate, thrombocyte, PRP, PRF, healing, animal model Journal: Veterinární medicína Pages: 567-570 Volume: 61 Issue: 10 Year: 2016 DOI: 10.17221/24/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/24/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201610-0005.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:10:id:24-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Ondruska Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic Author-Name: V. Parkanyi Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic Author-Name: J. Vasicek Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic Author-Name: R. Jurcik Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic Author-Name: E. Hanusova Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic Author-Name: D. Vasicek Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic Author-Name: A. Balazi Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic Author-Name: F. Vizzarri Author-Workplace-Name: University of Molise, Campobasso, Italy Title: Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine Abstract: The aim of this study was to evaluate the acute phase reaction and immune response of rabbits triggered by vaccination with a live attenuated myxoma virus (MXT) vaccine. Thirteen adult and 11 juvenile New Zealand white rabbit-based crossbreed rabbits, were used. Samples of rabbit peripheral blood were collected from vena auricularis centralis into heparinised tubes before vaccination and 48 h after vaccination. All animals were vaccinated by subcutaneous injection (0.5 ml) with a MXT vaccine. The blood plasma C-reactive protein level was measured by an ELISA kit using a double-antibody sandwich. For phenotyping of lymphocytes the fresh cells were stained with the following anti-rabbit monoclonal antibodies: anti-IgM, anti-CD4, anti-CD8 and anti-pan T2. Our results show that the use of attenuated myxoma virus vaccine significantly decreases the level of C-reactive protein in blood plasma of adult rabbits by 38.14% (P < 0.05) and of juvenile rabbits by 37.63% (P < 0.001), within 48 h. The rabbit C-reactive protein after MXT vaccination is a negative acute phase protein. In the group of adult rabbits the immune response to MXT vaccination was accompanied by a non-significant decrease in CD4+, pT2+, IgM+ subsets. On the other hand the values of CD8+, CD4+CD8+ and CD4+/CD8+ were non-significantly higher after MXT vaccination. Keywords: rabbit, Oryctolagus cuniculus, myxomatosis, ELISA Journal: Veterinární medicína Pages: 571-576 Volume: 61 Issue: 10 Year: 2016 DOI: 10.17221/159/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/159/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201610-0006.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:10:id:159-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A.J. Lora Author-Name: A.M. Molina Author-Name: C. Bellido Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: A. Blanco Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: J.G. Monterde Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Author-Name: M.R. Moyano Author-Workplace-Name: Veterinary Faculty, University of Cordoba, Cordoba, Spain Title: Adverse effects of bisphenol A on the testicular parenchyma of zebrafish revealed using histomorphological methods Abstract: The purpose of this study was to evaluate the consequences of bisphenol A (BPA) exposure in zebrafish (Danio rerio). Short-term effects at a testes level were assessed by means of different biomarkers in 60 male zebrafish exposed for 14 days in aquariums to different concentrations of BPA (1, 10, 100 and 1000 µg/l) in addition to a control batch. The LC-MS/MS method was used for the quantification of BPA. The BPA levels in the zebrafish tissues increased together with increasing exposure of fish to BPA. Several alterations were observed in the zebrafish testes. Particularly notable morphological changes included, a pronounced degeneration of all cell components, an increase in the percentage of the sustentacular (Sertoli) cells, and a marked decrease in the percentage of germ cells starting from concentrations of 100 µg/l of BPA. Our results indicate that histopathology and testes morphometry are sensitive biomarkers for the analysis of early effects of an environmental concentration of BPA in zebrafish experimental models. Our observations reveal that the nature and intensity of the morphological changes in the testes are concentration-dependent in the case of BPA, and manifest as adverse effects on the fish's reproductive health. Keywords: endocrine disruption, BPA, Danio rerio, sustentacular cells, spermatogonia, spermatocytes, spermatids, spermatozoa Journal: Veterinární medicína Pages: 577-589 Volume: 61 Issue: 10 Year: 2016 DOI: 10.17221/212/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/212/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201610-0007.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:10:id:212-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: G. Giusto Author-Workplace-Name: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy Author-Name: C. Bellino Author-Workplace-Name: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy Author-Name: M. Casalone Author-Workplace-Name: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy Author-Name: V. Caramello Author-Workplace-Name: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy Author-Name: F. Comino Author-Workplace-Name: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy Author-Name: M. Gandini Author-Workplace-Name: Department of Veterinary Sciences, University of Turin, Grugliasco, Italy Title: Mesh repair of a large ventral hernia with interposition of omentum in a calf: a case report Abstract: A one-month-old, Piedmontese female calf was admitted to the Department of Veterinary Sciences, University of Turin, for repair of a large ventral hernia. A large ventral hernia, approximately 20 cm long and 15 cm large was noticed extending from 3 cm caudal to the umbilicus down to the pubis. At ultrasonography the hernia content was represented by small intestine and omentum and no adhesions to the hernial sac could be detected. The hernial sac was composed by skin only. Because of the large dimensions of the defect and the economic value of the animal, surgical correction was recommended to the owner. A prosthetic implant with a polypropylene mesh was elected due to the dimension of the abdominal defect. The implant was placed intra-abdominally with the interposition of the omentum between the mesh and the underlying viscera. Two and six months after surgery follow-ups were performed and a positive outcome was confirmed. This is the first report of ventral hernia repair in large animals that combines the use of a tension-free polypropylene mesh with the interposition of the omentum between the viscera and the mesh. This procedure is safe, cost-effective and not associated with major complications. Keywords: bovine, soft tissue surgery, herniorraphy, adhesion, polypropylene Journal: Veterinární medicína Pages: 590-593 Volume: 61 Issue: 10 Year: 2016 DOI: 10.17221/272/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/272/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201610-0008.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:10:id:272-2015-VETMED