Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: VOLUME 61, CONTENTS; AUTHOR INDEX; AUTHOR INSTITUTION INDEX; LIST OF REVIEWERS Journal: Veterinární medicína Pages: I-XVI Volume: 61 Issue: 12 Year: 2016 DOI: 10.17221/9371-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/9371-VETMED.html File-Format: text/html Handle: RePEc:caa:jnlvet:v:61:y:2016:i:12:id:9371-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Bartkiene Author-Workplace-Name: Department of Food Safety and Quality, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: V. Krungleviciute Author-Workplace-Name: Department of Food Safety and Quality, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: R. Antanaitis Author-Workplace-Name: Department of Food Safety and Quality, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: J. Kantautaite Author-Workplace-Name: Department of Food Safety and Quality, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: A. Kucinskas Author-Workplace-Name: Department of Food Safety and Quality, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: M. Ruzauskas Author-Workplace-Name: Department of Food Safety and Quality, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: L. Vaskeviciute Author-Workplace-Name: Department of Food Safety and Quality, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: R. Siugzdiniene Author-Workplace-Name: Department of Food Safety and Quality, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: J. Kucinskiene Author-Workplace-Name: Department of Food Safety and Quality, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: J. Damasius Author-Workplace-Name: Department of Food research and Technology, Kaunas University of Technology, Kaunas, Lithuania Author-Name: G. Juodeikiene Author-Workplace-Name: Department of Food research and Technology, Kaunas University of Technology, Kaunas, Lithuania Title: Antimicrobial activity of lactic acid bacteria multiplied in an alternative substrate and their influence on physiological parameters of new-born calves Abstract: Here, the ability of Pediococcus pentosaceus and Pediococcus acidilactici to utilise potato tuber juice for cell synthesis without an external nutrient supplement was investigated, and the influence of lactic acid bacteria (LAB) grown in this substrate on the growth performance of new-born calves, as well as blood biochemical and faecal microbiological parameters was evaluated. Calves were selected based on the analogy principle, treatment group (n = 21), control group (n = 27). Calves in the treatment group were administered 50 ml of fermented potato tubers juice containing 9.6 log CFU/ml of LAB mixture for 14 days. Also, determination of antimicrobial activities of tested LAB against a variety of pathogenic and opportunistic bacterial strains previously isolated from diseased cattle was performed. It was found that LAB supernatants effectively inhibited the growth of Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Salmonella enterica, Corynebacter spp., Klebsiella pneomoniae, Enterococcus faecalis, and Bacillus cereus (the diameters of the inhibition zones varied between 11.0 ± 0.3 mm and 17.0 ± 0.6 mm). Thus, potato juice can be used as an alternative substrate for LAB cultivation (LAB cell concentration 9.6 ± 0.07 log CFU/ml). After lyophilisation (-48 °C) and spray-drying (+150 °C) viable cell concentrations in the fermented potato juice powder were 9.18 ± 0.09 log CFU/g and 9.04 ± 0.07 log CFU/g, respectively. The 50 ml of fermented potato tuber juice containing 9.6 log CFU/ml of LAB, administered every day for 14 days, reduced the risk of developing acidosis (stabilised blood pH; P < 0.05), reduced lactates and PCO2 concentration (P < 0.05) and the risk of liver lesions (reduced serum alanine aminotransferase concentration; P < 0.005) in blood and E. coli in the faeces of new-born calves. Keywords: alternative substrate, antimicrobial activity, blood parameters, calves, faecal parameters, lactic acid bacteria Journal: Veterinární medicína Pages: 653-662 Volume: 61 Issue: 12 Year: 2016 DOI: 10.17221/192/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/192/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201612-0002.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:12:id:192-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Kalender Author-Workplace-Name: Department of Bioengineering, University of Firat, Elazig, Turkey Author-Name: A. Kilic Author-Workplace-Name: Sivrice Vocational High School, University of Firat, Elazig, Turkey Title: Molecular characterisation of Shiga toxin-producing Escherichia coli O157:H7 isolates from cattle in eastern Turkey Abstract: Shiga toxin-producing Escherichia coli (STEC) O157:H7 is an important foodborne pathogen worldwide. In Turkey, little is known about the molecular characteristics of STEC O157:H7 isolates. The aim of this study was to examine the genetic diversity of STEC O157:H7 isolates from cattle in Turkey using pulsed-field gel electrophoresis (PFGE). A total of 18 STEC O157:H7 strains were isolated from 540 rectal swab samples of slaughtered cattle in two abattoirs (coded A and B) located in eastern Turkey. The presence of virulence genes (stx1, stx2, hlyA and eae) was detected using the polymerase chain reaction. Of the 18 STEC O157:H7 isolates, 14 had stx2, hlyA, and eae genes. Four of the isolates were positive for stx1, stx2, hlyA, and eae genes. Thirteen different PFGE patterns were observed among the STEC O157:H7 isolates. The most common PFGE pattern was X1, which was detected in six STEC O157:H7 isolates. These six isolates contained the same virulence genes (stx2, eae and hlyA). However, some of the isolates containing the same virulence genes showed different PFGE profiles. Eleven different PFGE patterns were detected among the isolates from abattoir B, and two different PFGE patterns were detected among the isolates from abattoir A. The results of PFGE revealed a wide genetic diversity among the STEC O157:H7 isolates from cattle in Turkey. This is the first study on PFGE typing of STEC O157:H7 isolates from cattle in Turkey and further studies are needed. Keywords: pulsed-field gel electrophoresis, virulence genes, bovine, rectal swabs, E. coli strains Journal: Veterinární medicína Pages: 663-668 Volume: 61 Issue: 12 Year: 2016 DOI: 10.17221/45/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/45/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201612-0003.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:12:id:45-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.M. Hossain Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Choongnam, Republic of Korea Author-Workplace-Name: Department of Animal Science, University of Manitoba, Winnipeg, Canada Author-Name: M. Begum Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Choongnam, Republic of Korea Author-Name: J.H. Park Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Choongnam, Republic of Korea Author-Name: S.J. Lee Author-Workplace-Name: CJ Global Food and Bio Company, Seoul, Republic of Korea Author-Name: K.H. Jang Author-Workplace-Name: CJ Global Food and Bio Company, Seoul, Republic of Korea Author-Name: Y.H. Hong Author-Workplace-Name: CJ Global Food and Bio Company, Seoul, Republic of Korea Author-Name: S.J. Cho Author-Workplace-Name: CJ Global Food and Bio Company, Seoul, Republic of Korea Author-Name: I.H. Kim Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Choongnam, Republic of Korea Title: Apparent total tract digestibility and ileal digestibility of dry matter, nitrogen, energy and amino acids in conventional, Bacillus subtilis-fermented and enzyme-treated soybean meal fed to weanling pigs Abstract: Apparent total tract digestibility (ATTD), apparent ileal digestibility (AID) and standardised ileal digestibility (SID) of dry matter, nitrogen, energy and amino acids were evaluated in six weanling barrows [(Landrace × Yorkshire) × Duroc] fed soybean meal diet (SBM), fermented soybean meal A diet with 50% solubility in potassium hydroxide (FSMA), fermented soybean meal B diet with 60% solubility in potassium hydroxide (FSMB), fermented soybean meal C diet with 70% solubility in potassium hydroxide (FSMC), enzyme-treated soybean meal (ETSM) and nitrogen-free diet. Pigs having body weights of 8.99 ± 0.40 kg were surgically equipped with T-cannulas of approximately 15 cm prior to the ileo-cecal junction and randomly allotted to one of five dietary treatments and a nitrogen-free diet in 6 × 6 Latin squares. With regards to the ATTD of essential amino acids, the ATTD of arginine, isoleucine, lysine, methionine and total essential amino acids was greater in pigs fed FSMB, FSMC, and ETSM diets than in SBM and FSMA diets. The ATTD of leucine, phenylalanine, threonine and valine in the FSMC diet was non-significant compared with the values of ATTD in FSMB and ETSM diets. With respect to non-essential amino acids the ATTD of aspartic acid, cysteine, glutamic acid, proline, tyrosine and total amino acids was greater in pigs fed the FSMC diet compared with the SBM diet and not different from the values of pigs fed the ETSM diet. The AID of dry matter, nitrogen and energy in the FSMC diet was greater (P < 0.05) than in pigs fed SBM and FSMA diets but was equal to the values of FSMB and ETSM diets. With regards to the AID of essential amino acids, the AID of histidine, lysine, methionine and threonine was greater in pigs fed FSMB, FSMC and ETSM diets than in SBM and FSMA diets (P < 0.05). The AID of isoleucine was greater in pigs fed FSMB and ETSM diets than the FSMA diet. The AID of arginine, phenylalanine and valine was greater in pigs fed the FSMC diet compared with the SBM diet. With respect to non-essential amino acids the AID of aspartic acid, glutamic acid, glycine, tyrosine and total amino acids was the same as in pigs fed FSMB, FSMC, ETSM diets and greater (P < 0.05) than in pigs fed SBM and FSMA diets. The SID of all essential amino acids in pigs fed the FSMC diet was greater than for the FSMA diet. In the case of non-essential amino acids, the SID of all amino acids except for cysteine was greatest in pigs fed FSMB, FSMC and ETSM treatments. In conclusion, dietary supplementation of FSMB, FSMC and/or ETSM diets can improve the ATTD, AID and SID of dry matter, nitrogen, energy, total amino acids and most of the essential amino acids in weanling pigs. Keywords: growth performance, fermented soybean meal, anti-nutritional factors, antigens, lectins, trypsin inhibitors, Bacillus subtilis, T-cannula, surgery Journal: Veterinární medicína Pages: 669-680 Volume: 61 Issue: 12 Year: 2016 DOI: 10.17221/389/2014-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/389/2014-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201612-0004.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:12:id:389-2014-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. Nofouzi Author-Workplace-Name: Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Author-Name: M. Aghapour Author-Workplace-Name: Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Author-Name: Gh. Hamidian Author-Workplace-Name: Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Author-Name: F. Katiraee Author-Workplace-Name: Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Author-Name: J. Stanford Author-Workplace-Name: Windeyer Institute of Medical Sciences, University College London, London, United Kingdom Author-Name: P. Ripley Author-Workplace-Name: BioEos Ltd., Mill House, Claygate, Marden, Kent, United Kingdom Title: Oral administration of heat killed Tsukamurella inchonensis enhances immune responses and intestinal function in mice Abstract: We studied the effects of heat-killed Tsukamurella inchonensis on immune parameters and intestinal structure in mice. Mice were treated with three doses of bacteria (5 × 107, 1 × 108 and 2 × 108 CFU/mouse) consecutively for seven days. Body weight, delayed type hypersensitivity response, relative organ weight, and haemagglutination titres were studied in different groups of animals. Villus height, villus width, villus/crypt ratio, crypt depth and goblet cell and intestinal epithelial lymphocyte density in villi were also determined. There was no significant increase in liver, spleen and kidney weights at any dose. Chicken red blood cell was used as a model antigen in the humoral and cellular immune response tests and Tsukamurella inchonensis elicited a significant (P < 0.05) increase in the delayed type hypersensitivity response at doses of 2 × 108 CFU/mouse. In the haemagglutination titres test, Tsukamurella inchonensis showed a modulatory effect at a dose of 2 × 108 CFU/mouse. There were clear increases in the height of villi and depth of crypts in all three treated groups, but no significant effects on villus/crypt ratio and villus width. Goblet cell density was increased significantly only in high dose-treated mice, while intestinal epithelial lymphocyte density was increased significantly in medium and high dose-treated mice. Overall, Tsukamurella inchonensis showed a stimulatory effect on immune functions and enhanced immune barrier function in the intestines of mice. Keywords: immunomodulation, delayed type hypersensitivity, haemagglutination titre, intestine, villus, histology, liver, spleen, kidney Journal: Veterinární medicína Pages: 681-688 Volume: 61 Issue: 12 Year: 2016 DOI: 10.17221/82/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/82/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201612-0005.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:12:id:82-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.H. Seok Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Workplace-Name: Gyeongnam Wildlife Center, Jinju, Republic of Korea Author-Name: D.H. Jeong Author-Workplace-Name: Species Restoration Technology Institute of Korea National Park Service, Gurye, Republic of Korea Author-Name: H.C. Lee Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: I.H. Hong Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: S.C. Yeon Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Workplace-Name: Gyeongnam Wildlife Center, Jinju, Republic of Korea Title: Evaluation of diagnostic coelioscopy including liver and kidney biopsies in cinereous vultures (Aegypius monachus) Abstract: Diagnostic coelioscopy, including liver and kidney biopsies, was performed in seven cinereous vultures (Aegypius monachus). A 5-mm endoscopy system was used for examination of coelomic viscera. The endoscopist rated the ease of entry into the coelom and visualisation. Coelioscopic biopsy was performed using a 5-mm biopsy forceps following the diagnostic coelioscopy, and the diagnostic quality of the samples was evaluated. The endoscopic entry and visualisation scores ranged from satisfactory to excellent for all coelomic structures, except for the oesophagus, spleen, epididymis/oviduct and pancreas in all vultures. The coelioscopic examinations of coelomic structures and biopsy samples were carried out safely and easily. The biopsy samples were suitable for histopathological examination. Thus, minimally invasive coelioscopy using a 5-mm endoscopy system can be considered a useful technique suitable for visceral examination of large raptors such as cinereous vultures. Keywords: biopsy, avian endoscopy, minimally invasive endosurgery, large raptor Journal: Veterinární medicína Pages: 689-700 Volume: 61 Issue: 12 Year: 2016 DOI: 10.17221/103/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/103/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201612-0006.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:12:id:103-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J.H. Park Author-Name: S.I. Lee Author-Name: I.H. Kim Author-Workplace-Name: Department of Animal Resource and Science, Dankook University, Cheonan, Republic of Korea Title: Effects of lupin seed supplementation on egg production performance, and qualitative egg traits in laying hens Abstract: We investigated the effects of lupin (Lupinus angustifolius L.) seed supplementation on egg production performance and egg quality in laying hens. A total of 120 Hy-Line Brown laying hens (29-week old) were used in a nine week trial. Laying hens were sorted into pens with six birds per pen and five pens per treatment. Treatments were (1) control, corn-soybean meal-based control diets, (2) 11% lupin (control + 11% lupin), (3) 16.5% lupin (control + 16.5% lupin), and (4) 22% lupin (control + 22% lupin). The average daily feed intake of laying hens fed with lupin diets was increased compared with control at Week 6 (P < 0.05). The egg production rate was higher in lupin diets than in the control diet at Week 3, 4, and 5 (P < 0.05). Supplementation with lupin improved the egg production rate linearly at Week 3, 4, 5, and 9, as dietary lupin increased from 11 to 22%. Laying hens fed with 11% and 16.5% lupin diets had higher rates of extra-large-sized egg production at Week 1 (quadratic, P = 0.04). Yolk colour was higher in hens fed lupin diets than in those fed with the control at Week 9 (P < 0.05). Supplementation of lupin in laying hen diets linearly improved the yolk colour (P < 0.05) at Week 5. In conclusion, supplementation of the diet with lupin can improve egg production and yolk colour with no apparent effect on average daily feed intake and eggshell quality in laying hens. Keywords: Lupinus angustifolius L., laying performance, egg quality, yolk colour Journal: Veterinární medicína Pages: 701-709 Volume: 61 Issue: 12 Year: 2016 DOI: 10.17221/330/2014-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/330/2014-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201612-0007.txt Handle: RePEc:caa:jnlvet:v:61:y:2016:i:12:id:330-2014-VETMED