Template-Type: ReDIF-Article 1.0 Author-Name: A.R. Hosseindoust Author-Workplace-Name: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea Author-Name: S.H. Lee Author-Workplace-Name: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea Author-Name: J.S. Kim Author-Workplace-Name: Southern Research and Outreach Center, Minnesota, USA Author-Name: Y.H. Choi Author-Workplace-Name: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea Author-Name: H.S. Noh Author-Workplace-Name: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea Author-Name: J.H. Lee Author-Workplace-Name: CTCBIO Inc., Seoul, Republic of Korea Author-Name: P.K. Jha Author-Workplace-Name: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea Author-Name: I.K. Kwon Author-Workplace-Name: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea Author-Name: B.J. Chae Author-Workplace-Name: College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea Title: Dietary bacteriophages as an alternative for zinc oxide or organic acids to control diarrhoea and improve the performance of weanling piglets Abstract: In this study, the antibacterial substances ZnO, organic acids and a bacteriophage cocktail were added to the diet of weanling pigs to evaluate the effects on gut health. Dietary treatments were basal diet without any supplementation (Control) and basal diet either supplemented with 0.34% (2500 ppm) ZnO, 0.20% organic acids or with 0.10% bacteriophage cocktail. Faecal score was decreased in ZnO and bacteriophage cocktail treatments. The total number of ileal anaerobic bacteria, Bifidobacterium spp. and Lactobacillus spp. were higher in ZnO, bacteriophage cocktail and organic acids, while ileal coliforms and caecal Clostridium spp. were decreased in comparison to Control. Faecal coliforms (Day 7 and Day 21) and Clostridium spp. (Day 21 and Day 35) were lower in bacteriophage cocktail. The gain to feed ratio was improved in all supplemented groups. The digestibility of dry matter was increased at the end of the experiment in all supplemented groups, while that of crude protein was increased only at Day 21 in bacteriophage cocktail. Duodenal villus height was increased in ZnO and bacteriophage cocktail. Bacteriophage cocktail also showed a greater villus height in the small intestine. Supplementation of bacteriophage cocktail in weaning pig diets resulted in better growth performance, digestibility and gut development compared to Control, and thus, it can be concluded that its effects are comparable to ZnO or organic acids supplementation. Keywords: growth performance, intestinal morphology, microbiota, faecal score, coliforms, Clostridium Journal: Veterinární medicína Pages: 53-61 Volume: 62 Issue: 2 Year: 2017 DOI: 10.17221/7/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201702-0001.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:2:id:7-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V. Schweinzer Author-Workplace-Name: Clinic for Ruminants, University of Veterinary Medicine, Vienna, Austria Author-Name: M. Iwersen Author-Workplace-Name: Clinic for Ruminants, Clinical Unit for Herd Health Management in Ruminants, Vienna, Austria Author-Name: M. Drillich Author-Workplace-Name: Clinic for Ruminants, Clinical Unit for Herd Health Management in Ruminants, Vienna, Austria Author-Name: T. Wittek Author-Workplace-Name: Clinic for Ruminants, University of Veterinary Medicine, Vienna, Austria Author-Name: A. Tichy Author-Workplace-Name: Department of Biomedical Sciences, Platform for Bioinformatics and Biostatistics, University of Veterinary Medicine, Vienna, Austria Author-Name: A. Mueller Author-Workplace-Name: Idexx Laboratories, Ludwigsburg, Germany Author-Name: R. Krametter-Froetscher Author-Workplace-Name: Clinic for Ruminants, University of Veterinary Medicine, Vienna, Austria Title: Macromineral and trace element supply in sheep and goats in Austria Abstract: The aim of this study was to determine the supply of 25 different macrominerals (calcium, magnesium, potassium) and trace elements (aluminium, arsenic, barium, boron, cadmium, cobalt, copper, iron, lithium, lead, manganese, molybdenum, nickel, selenium, silicon, strontium, sulphur, thallium, tin, titanium, uranium, zinc), and to ascertain the presence of any over- or undersupplies. As a second objective, we undertook a comparison of our results with existing reference values from selected literature and from laboratory analyses, with the aim of classifying the obtained results into the following categories: 'deficiency', 'adequate' and 'oversupply'. For the study, 16 sheep and four goat farms located in the Austrian states of Upper Austria (n = 12), Carinthia (n = 6) and Vorarlberg (n = 2) were selected. From every farm, five serum blood samples were obtained by puncturing the vena jugularis to evaluate the macromineral and trace element status in clinically sound female sheep (n = 80; 12 different breeds) and female goats (n = 20; Saanen goats, Boer goats). The animals were kept for dairy farming (milking and/or meat production) or for landscaping. The mean age of both sheep and goats was 3.1 years (sheep: min. 0.5, max. 10; goats: min. 1, max. 5); 44% of the studied animals were lactating and 22% were pregnant at the time of sampling. The serum blood samples were sent to a laboratory and analysed using inductively coupled plasma optical emission spectrometry and inductively coupled plasma mass spectrometry. In summary, the supply with macrominerals and trace elements compared with reference values from the laboratory was adequate for As, Ca, Fe and Mg in sheep and for As, Ca, Cu, K, Mg and Se in goats. Although all animals in our study were examined for clinical signs of disease by the local veterinarian, oversupplies in sheep for the elements K and Mo and in goats for Fe as well as undersupplies in sheep and goats for Zn could be found in the serum of the studied animals. Keywords: small ruminant, minerals, element status, deficiency, oversupply, serum, reference value, ICP-MS, IPC-OES Journal: Veterinární medicína Pages: 62-73 Volume: 62 Issue: 2 Year: 2017 DOI: 10.17221/243/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/243/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201702-0002.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:2:id:243-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Sirivisoot Author-Workplace-Name: Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand Author-Name: S. Techangamsuwan Author-Workplace-Name: Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand Author-Name: S. Tangkawattana Author-Workplace-Name: Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand Author-Name: A. Rungsipipat Author-Workplace-Name: Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand Title: Pax5 as a potential candidate marker for canine B-cell lymphoma Abstract: Immunophenotyping is a valuable method for prognosis in canine malignant lymphoma. The general B-cell marker is CD79a; however, Pax5 or B-cell specific activator protein, a transcription factor that controls B-cell identity and cell maturation, could also be used as a B-cell indicator in canine lymphomas. This study aimed to use Pax5, CD79a and CD3 expression in immunohistochemistry of spontaneous canine lymphomas, in order to carry out diagnosis and histopathological classification according to the World Health Organization guidelines. Forty-six retrospective cases including 33 multicentric, eight extranodal, and five alimentary lymphomas in dogs were immunostained by anti-Pax5 and anti-CD79a antibodies for B-cell identification, and anti-CD3 antibody for T-cell identification. T-cell lymphomas (CD3+/Pax5-/CD79a-) accounted for 30.43% of cases (14/46), and four of the lymphomas (28.57%) presented with CD3+/Pax5-/CD79a+. Conversely, B-cell lymphomas (CD3-/Pax5+/CD79a+) accounted for 69.57% of cases (32/46) and 12.5% of these (4/32) showed only Pax5-positive cells (CD3-/Pax5+/CD79a-). Therefore, in dogs, Pax5 appears to be a more useful marker for staining all B-cell subtypes compared to CD79a. Immunophenotyping with both Pax5 and CD3 are necessary for lymphoid lineage identification in canine lymphomas. Keywords: B-cell marker, dog, immunohistochemistry, lymphoma Journal: Veterinární medicína Pages: 74-80 Volume: 62 Issue: 2 Year: 2017 DOI: 10.17221/100/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/100/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201702-0003.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:2:id:100-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O. Melter Author-Workplace-Name: 2nd Medical Faculty, Charles University, Prague, Czech Republic Author-Name: P. Svec Author-Workplace-Name: Faculty of Science, Masaryk University, Brno, Czech Republic Author-Name: J. Tkadlec Author-Workplace-Name: 2nd Medical Faculty, Charles University, Prague, Czech Republic Author-Name: J. Doskar Author-Workplace-Name: Faculty of Science, Masaryk University, Brno, Czech Republic Author-Name: H. Kinska Author-Workplace-Name: Laboratory of Veterinary Medicine (Labvet), Prague, Czech Republic Author-Name: R. Pantucek Author-Workplace-Name: Faculty of Science, Masaryk University, Brno, Czech Republic Title: Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR Abstract: Staphylococcus pseudintermedius is a genuine opportunistic pathogen of the skin, especially in canids. However, characterisation of virulence, antimicrobial resistance and genotypic variability in methicillin-susceptible S. pseudintermedius isolates has not been fully explored. In this study, coagulase-positive staphylococcal isolates collected from dogs of various breeds and ages suffering from dermatitis (n = 70), pyoderma (n = 7), and otitis (n = 7), from districts of Prague (Czech Republic) and surrounding areas, were characterised using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and repetitive sequence-based PCR fingerprinting. Susceptibility to antimicrobial agents was determined, virulence factor genes for leukocidin (lukSF-I), exfoliatins (exi, expB, and siet), enterotoxin C (seccanine) and enterotoxin-related genes (se-int and sel) were detected using multiplex PCR and the genotypes of S. pseudintermedius isolates were determined using SmaI macrorestriction analysis. The majority of the staphylococcal isolates (n = 84) were identified as S. pseudintermedius (n = 79) and all of them were susceptible to methicillin/oxacillin (MSSP). About half of the strains (n = 41) were resistant to macrolide-lincosamide-streptogramin B antimicrobial agents and resistance was mediated in all but one of the strains by the erm(B) gene. The genes for lukSF-I, siet, se-int, and sel were detected in the majority of the MSSP strains (96.2%, 100%, 100%, and 73.4%, respectively). Investigated canine S. pseudintermedius isolates were highly heterogeneous, which prevented the correlation of any specific lineage to a particular infection, dog breed, or region of origin. Keywords: Staphylococcus pseudintermedius, macrolide lincosamide-streptogramin B (MLSB) resistance, genotyping, virulence genes Journal: Veterinární medicína Pages: 81-89 Volume: 62 Issue: 2 Year: 2017 DOI: 10.17221/105/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/105/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201702-0004.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:2:id:105-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V. Stancova Author-Workplace-Name: Department of Animal Protection, Welfare and Behaviour, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: L. Plhalova Author-Workplace-Name: Department of Animal Protection, Welfare and Behaviour, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: J. Blahova Author-Workplace-Name: Department of Animal Protection, Welfare and Behaviour, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: D. Zivna Author-Workplace-Name: Department of Animal Protection, Welfare and Behaviour, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: M. Bartoskova Author-Workplace-Name: Department of Animal Protection, Welfare and Behaviour, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: Z. Siroka Author-Name: P. Marsalek Author-Workplace-Name: Department of Animal Protection, Welfare and Behaviour, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: Z. Svobodova Author-Workplace-Name: Department of Animal Protection, Welfare and Behaviour, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Title: Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca) Abstract: In the present study, the effects of sub-lethal sub-chronic doses of ibuprofen, diclofenac, and carbamazepine alone, and in combination (concentration range 0.02-60 µg/l), on the early life stages of tench (Tinca tinca) were investigated. The lower concentrations of pharmaceuticals tested (0.02, 0.2, 2 µg/l) represent the concentration values of these substances commonly present in surface waters or effluents from wastewater treatment plants. Multiple biomarkers of biotransformation, antioxidant defence systems, and lipid peroxidation were determined in fish after 35 days of exposure. The evaluated pharmaceuticals induced oxidative stress in fish both alone and in combination with each other. Generally, 60 µg/l of each single pharmaceutical influenced the activity of antioxidant enzymes significantly (P < 0.05), whereas the same concentration of these pharmaceuticals in combination (1 : 1 : 1) did not have any impact on the activity of these enzymes. However, changes in biotransformation and antioxidant enzymes were apparent if lower concentrations of these pharmaceuticals were administered in the mixture. Significant changes (P < 0.05) in the activities of glutathione reductase, glutathione peroxidase, and glutathione-S-transferase were observed even at environmental concentration ranges. A significant effect (P < 0.05) on lipid peroxidation levels was found only in the experimental group exposed to carbamazepine. Keywords: antioxidant defence system, lipid peroxidation Journal: Veterinární medicína Pages: 90-97 Volume: 62 Issue: 2 Year: 2017 DOI: 10.17221/125/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/125/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201702-0005.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:2:id:125-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H.Y. Yoon Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: J.W. Choi Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: Ji H. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: Jung H. Kim Author-Workplace-Name: Veterinary Medical Teaching Hospital, Konkuk University, Seoul, Republic of Korea Title: Use of a double-wire woven uncovered nitinol stent for the treatment of refractory tracheal collapse in a dog: a case report Abstract: A 2.7 kg, 11-year-old, castrated male Maltese dog was presented for evaluation of a 2-year history of intractable coughing, dyspnoea and cyanosis. A diagnosis of tracheal collapse with myxomatous mitral valve disease was made on the basis of inspiratory and expiratory thoracic radiographs, fluoroscopy and echocardiography. Measurement for stent size selection was performed on thoracic radiographs. A 10 mm (diameter) × 70 mm (length) self-expanding double-wire woven uncovered nitinol stent was used for intratracheal implantation and was deployed under fluoroscopic guidance. On thoracic radiography seven days after surgery, the position of the stent remained unchanged. On presentation six months after surgery, the owner reported that the dog was doing well without medical management. Although studies of various intraluminal stents have been reported in dogs, to the authors' knowledge, use of a double-wire woven uncovered nitinol stent has not been reported previously for the management of a dog with tracheal collapse. Since this particular type of stent with unfixed individual cells provided proper airway patency without stent fracture in the dog in this report, this stent might be used as an alternative to other commercially available nitinol stents in cases of thoracic inlet collapse. Keywords: canine, collapsing trachea, intraluminal tracheal stenting Journal: Veterinární medicína Pages: 98-104 Volume: 62 Issue: 2 Year: 2017 DOI: 10.17221/15/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/15/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201702-0006.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:2:id:15-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Hajek Author-Workplace-Name: Small Animal Referral Centre Sibra, Bratislava, Slovak Republic Author-Name: V. Simerdova Author-Workplace-Name: Small Animal Referral Centre Sibra, Bratislava, Slovak Republic Author-Name: M. Vavra Author-Workplace-Name: Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: C.F. Agudelo Author-Workplace-Name: Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Title: Toxic encephalopathy associated with high-dose metronidazole therapy in a dog: a case report Abstract: This case report describes an episode of acute ataxia, tremor, vertical nystagmus and progressive weakness in a mixed breed dog treated with high doses of metronidazole. Complete blood cell count, serum biochemistry, coagulation profile, blood pressure measurement, urinalysis, computed tomography of the brain and cerebrospinal fluid examination were unremarkable. Metronidazole had been administered at a dose of 65 mg/kg/day and neurotoxicity was, therefore, suspected. Drug concentrations in the patient's serum and cerebrospinal fluid were measured using high-performance liquid chromatography and compared to control dogs. Metronidazole administration was immediately discontinued; supportive care consisted of fluid therapy and diazepam treatment. The neurological status of the patient improved rapidly within 72 h. The aim of this case report is to describe the clinical presentation of metronidazole intoxication in a mixed breed dog and to interpret the chromatographic analysis which can be a beneficial diagnostic and screening tool in dogs intoxicated with metronidazole. Keywords: vestibular diseases, cerebellar dysfunction, canine diseases, gamma-aminobutyric acid, chromatography Journal: Veterinární medicína Pages: 105-110 Volume: 62 Issue: 2 Year: 2017 DOI: 10.17221/264/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/264/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201702-0007.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:2:id:264-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Y. Zhalniarovich Author-Name: A. Przeworski Author-Workplace-Name: Department of Surgery and Radiology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: J. Glodek Author-Workplace-Name: Department of Surgery and Radiology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: Z. Adamiak Author-Workplace-Name: Department of Surgery and Radiology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Title: Low-field magnetic resonance imaging of otitis media in two cats: a case report Abstract: Otitis media is a common disease in clinical veterinary practice. Although low-field magnetic resonance imaging reports for otitis media in dogs exist, in cats detailed information is missing. Radiography is helpful in diagnosing chronic inflammation, but may be insufficient during the initial phase of inflammation. For this reason, this report describes the magnetic resonance findings in two cats with otitis media. In both cases, middle ear empyema was detected. Magnetic resonance imaging of middle ear disorders in cats should contain pre- and post-contrast T1-weighted sequences in the dorsal and transverse planes, a T2-weighted sequence in the dorsal and transverse planes and a fluid-attenuated inversion recovery sequence in the dorsal or transverse planes. On pre-contrast T1-weighted images, the empyema had an intensity similar to that of brain tissue with a delicate hyper-intensity in the middle. On post-contrast T1-weighted images, the material had non-uniform enhancement in the dorsolateral compartment and circumference enhancement in the ventromedial compartment of the tympanic bulla with a hypo-intense centre. On T2-weighted images, the mass had heterogeneously increased signal intensity to brain tissue, but was less intense than cerebrospinal fluid. In the fluid-attenuated inversion recovery sequence, the pathological lesion was distinctly hyper-intense in comparison to other tissues with a narrow area of increased signal intensity in the middle of the ventromedial tympanic bulla compartment. Magnetic resonance imaging is commonly used for the visualisation of different disorders of the membranous labyrinth and allows the differentiation of chronic hematomas, empyemas and middle and internal ear neoplasia. The recommended magnetic resonance protocol of the middle ear should include pre- and post-contrast T1 sequences in the dorsal and transverse planes, the T2 sequence in the dorsal and transverse planes and the fluid-attenuated inversion recovery sequence in the dorsal or transverse planes. Keywords: MRI, middle ear, tympanic bulla, empyema, feline Journal: Veterinární medicína Pages: 111-115 Volume: 62 Issue: 2 Year: 2017 DOI: 10.17221/94/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/94/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201702-0008.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:2:id:94-2016-VETMED