Template-Type: ReDIF-Article 1.0 Author-Name: S. Yesilmen Author-Name: A. Vural Author-Workplace-Name: Faculty of Veterinary Medicine, University of Dicle, Diyarbakir, Turkey Author-Name: M.E. Erkan Author-Workplace-Name: Faculty of Veterinary Medicine, University of Dicle, Diyarbakir, Turkey Author-Name: I.H. Yildirim Author-Workplace-Name: Faculty of Veterinary Medicine, University of Dicle, Diyarbakir, Turkey Title: Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method Abstract: In this study, the presence of Arcobacter spp. was investigated in the faeces of cattle, sheep, goats, dogs and cloacal swab samples of chickens using the 16S rDNA-RFLP method. The prevalence of Arcobacter in these species was found to be 13%, 12%, 16%, 4% and 33%, respectively. On the other hand, Arcobacter spp. could not be isolated from rabbit faeces. A total of 78 (13%) Arcobacter spp. isolates were obtained from the 500 faecal samples and 100 cloacal swab samples examined in this study. From these 78 Arcobacter isolates, 24 (30.8%), 20 (25.6%), 11 (14.1%), 8 (10.7%), 4 (5.1%), 3 (3.9%) and 2 (2.6%) were identified by 16S rDNA-RLFP as A. cryaerophilus, A. butz- leri, A. skirrowii, A. cloacae, A. cibarius, A. halophilus, and A. nitrofigilis, respectively. All A. cryaerophilus (n = 24) isolates were found to be resistant to cloxacillin; all A. butzleri (n = 20) and A. skirrowii isolates were found to be resistant to penicillin/novobiocin, cefoperazone, tetracycline and cloxacillin. It was determined in this study that clinically healthy cattle, sheep, goats, dogs and chickens are reservoirs of Arcobacter spp. Keywords: cattle, sheep, goats, dogs, chickens, rabbits, antibiogram Journal: Veterinární medicína Pages: 301-307 Volume: 62 Issue: 6 Year: 2017 DOI: 10.17221/69/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/69/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201706-0001.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:6:id:69-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B.B. Yagci Author-Workplace-Name: Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey Author-Name: N. Ocal Author-Workplace-Name: Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey Author-Name: S. Yasa Duru Author-Workplace-Name: Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey Author-Name: M. Akyol Author-Workplace-Name: Department of Biostatistics, Yildirim Beyazit University, Ankara, Turkey Title: The efficacy of a combination of azithromycin and toltrazuril for the treatment of calves naturally infected with cryptosporidiosis: a randomised, double-blind, placebo-controlled comparative clinical trial Abstract: Cryptosporidium spp. are important emerging pathogens that can cause infections in humans, especially in immunosuppressed patients. Treatment of diseased calves that shed the infectious stage of the protozoon is critically important to prevent contamination by Cryptosporidium spp. oocysts. The objective of this study was to determine if a combination of azithromycin and toltrazuril provides a better treatment option for calf cryptosporidiosis compared to the use of either agent alone. A total of 55 Holstein calves with cryptosporidiosis were randomly assigned into four groups. Group A (n = 15) received azithromycin at a dose of 20 mg/kg/day per os for six days, group T (n = 15) received toltrazuril at a dose of 20 mg/kg per os every other day on a total of three occasions, and group AT (n = 15) received the combination of both drugs. The control group (n = 10) received purified water. A randomised, placebo-controlled, double-blinded clinical study was designed. The number of oocysts in faeces and clinical parameters were followed daily. Selected haematological and biochemical parameters were measured at the beginning and end of the study. The calves receiving the combination of azithromycin and toltrazuril exhibited a better clinical score as well as the lowest number of oocysts at all time-points. In conclusion, the combination of azithromycin and toltrazuril promotes rapid clinical recovery in calves infected with cryptosporidiosis and stops oocyst shedding. Thus, the combination of azithromycin and toltrazuril is an effective alternative treatment option for calf cryptosporidiosis. Keywords: neonatal, cattle, diarrhoea, apicomplexan, therapy Journal: Veterinární medicína Pages: 308-314 Volume: 62 Issue: 6 Year: 2017 DOI: 10.17221/125/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/125/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201706-0002.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:6:id:125-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Pyszkova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Pyszko Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: V. Paral Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: F. Tichy Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Kyllar Title: A radiographic and anatomic study of caudolateral curvilinear osteophytes on the canine femoral neck Abstract: Caudolateral curvilinear osteophytes (CCOs) are considered to be an important finding for the early detection of canine hip dysplasia. The objective of this study was to determine the association between the radiographic and anatomic appearance of CCOs, and to determine whether CCOs are indicative of osteoarthritis. One hundred canine femurs were used to determine the topographical location of CCOs on the femoral neck, and the anatomic and radiographic presence of CCOs and their association with weight, sex, osteoarthritis, and femoral morphometry. Three angles were calculated to assess femoral conformation. The alpha angle and gamma angle described the angulation of the proximal femur and femoral neck, respectively. The beta angle described the angulation of the distal femur. Anatomic CCO was not detected in four femurs. No radiographic CCO was detected in 35 femurs and osteoarthritis changes were not found in 30 femurs. Statistically significant relationships were observed between anatomic CCO, alpha angle, and gamma angle. Statistically significant associations were observed between radiographic CCO, alpha angle, and gamma angle. The results of this study support an association between radiographic CCO on the femoral neck and osteoarthritis changes in the proximal femur. It can also be concluded that CCOs develop in association with femoral neck angulation, and may therefore be considered as an adaptation to the forces created by body weight that act through the femoral neck. Keywords: hip laxity, femoral luxation, hip dysplasia, osteoarthritis Journal: Veterinární medicína Pages: 315-324 Volume: 62 Issue: 6 Year: 2017 DOI: 10.17221/2/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201706-0003.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:6:id:2-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.Y. Heo Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University Specialized Campus, Iksan, Republic of Korea Author-Name: H.Y. Kim Author-Workplace-Name: Department of BIN Fusion Technology, Chonbuk National University, Jeonju, Republic of Korea Author-Name: N.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University Specialized Campus, Iksan, Republic of Korea Title: Evaluation of poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of critical-sized segmental bone defects in a canine model Abstract: The treatment of segmental bone defects is a challenging problem for both human and veterinary medicine. Various biomaterials have successfully been used to treat these defects. Numerous recent in vitro studies have shown the potential of treating bone tissues using poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres, which are fabricated using electrospinning. The purpose of this study was to evaluate the possibility of using a bone scaffold of PLGA/HAp nanofibres to repair critical-sized segmental bone defects in a canine model. The experimental bone defects were created in a 15 mm-long region of the radius. The area of the defect in each of 10 Beagle dogs was treated with a transplant of PLGA/HAp nanofibres in gelatin. The control group consisted of five Beagle dogs with similar defect sites that were not treated. Radiological and histological examinations were used to monitor the response of PLGA/HAp nanofibre-treated canine bone. Micro-computed tomography (micro-CT) was used to evaluate bone mass parameters 18 weeks after treatment in the experimental bone defect group. Our radiological and histological results showed that the PLGA/HAp nanofibre is biodegradable in the defect sites and replaces new bone tissue. Micro-CT showed that bone mass parameters were significantly (P < 0.05) increased in the critical-sized segmental bone defects of PLGA/HAp nanofibre-treated animals as compared to those of untreated animals. Based on these results, we conclude that PLGA/HAp nanofibres may be used as a bone scaffold biomaterial in canines. Keywords: poly(lactide-co-glycolide), hydroxyapatite, nanofiber, electrospinning, critical-size canine defect model Journal: Veterinární medicína Pages: 325-332 Volume: 62 Issue: 6 Year: 2017 DOI: 10.17221/283/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/283/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201706-0004.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:6:id:283-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: X.Y. Hu Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China Author-Name: L. Luan Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China Author-Name: W. Guan Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China Author-Name: J. Shi Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China Author-Name: Y.B. Zhao Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China Author-Name: H.G. Fan Title: Tolfenamic acid and meloxicam both provide an adequate degree of postoperative analgesia in dogs undergoing ovariohysterectomy Abstract: This study was aimed at comparing the postoperative analgesic effects of tolfenamic acid and meloxicam in dogs undergoing ovariohysterectomy. Ovariohysterectomy was performed in 24 female dogs. All dogs were administered pre-anaesthetic medication comprised of 0.02 mg/kg i.m. acepromazine, and general anaesthesia was induced with i.v. propofol (4-6 mg/kg) and maintained with 1.5-2.0% isoflurane. Dogs were divided into three groups (n = 8). Following induction of anaesthesia, group C received 0.05 ml/kg sterile saline i.m.; group T received 4 mg/kg tolfenamic acid i.m.; group M received 0.2 mg/kg meloxicam s.c. Heart rate, respiratory rate, rectal temperature, mean arterial pressure and arterial oxygen saturation of haemoglobin were monitored intraoperatively. Pain was assessed using the short form of the Glasgow composite pain scale (SF-GCPS) by two observers who were blinded to the treatment groups; pain was assessed at the time of pre-medication (baseline), and at 2, 4, 6, 8, 12 and 24 h after extubation. Rescue analgesia (0.2 mg/kg i.m. methadone) was administered to any dog with an SF-GCPS score of greater than or equal to six during postoperative monitoring. The pain score in group C was significantly higher compared with group T and group M at 4, 6 and 8 h, while there were no significant differences between the two treatment groups. The mean pain score in group C was also higher than that in group M at 2 h. Rescue analgesia was first administered at 4 h in group C. Rescue analgesia was required by significantly more dogs in group C (n = 8) compared with groups T (n = 0) and M (n = 1), but there was no significant difference between the two treatment groups. Thus, tolfenamic acid and meloxicam provide adequate postoperative analgesia to similar degrees over 24 h in healthy dogs undergoing ovariohysterectomy. Keywords: postoperative analgesia, dogs, assessment, pain, SF-GCPS score Journal: Veterinární medicína Pages: 333-341 Volume: 62 Issue: 6 Year: 2017 DOI: 10.17221/143/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/143/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201706-0005.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:6:id:143-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: C.S. Lin Author-Workplace-Name: School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan Author-Name: G.H. Chiang Author-Workplace-Name: School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan Author-Name: C.H. Liu Author-Workplace-Name: School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan Author-Name: H.C. Tsai Author-Workplace-Name: ProtectLife International Biomedical, Inc. Author-Name: C.C. Yang Author-Workplace-Name: ProtectLife International Biomedical, Inc. Author-Name: K. Chen Author-Workplace-Name: UHSIO Taiwan, Inc. Author-Name: S.K. Huang Author-Workplace-Name: UHSIO Taiwan, Inc. Author-Name: T.L. Cheng Author-Workplace-Name: College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan Author-Name: C.C. Chou Author-Workplace-Name: College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan Title: Comparison of a full-spectrum multi-analyte clinical analyser with six reference instruments using canine and feline blood samples Abstract: In this study, we report the characterisation of a novel centrifugation and spectrum-integrated veterinary clinical analyser, the AmiShieldTM, which has been developed for the multiplex measurement of biochemical, electrolyte and immunoassay parameters in a point-of-care testing environment. The aims of this study were to evaluate the analytical performance of the AmiShieldTM and to compare it with six reference instruments using clinical blood samples. Two hundred and four canine and 120 feline blood samples collected from veterinary teaching hospitals were analysed in parallel using the AmiShield and appropriate reference instruments. All results were evaluated separately for canine and feline specimens. The instrument's analytical performance was evaluated initially for short- and long-term precision, bias, and observed total error using quality control material. This was followed by comparison of clinical specimens on the AmiShield analyser in parallel with the Vitros and Hitachi for biochemical parameters, VetScan and SNAPshot for total bile acids, and VetLyte and Biolyte for electrolytes. Overall, the AmiShield analyser's performance met the standards of the American Society for Veterinary Clinical Pathology for total allowable error for most analytes, and can be considered suitable for use in veterinary clinical practices. Using canine samples, excellent correlation coefficients (r ≧ 0.92) were identified for 14 analytes of various categories including glucose, total protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, phosphorus, Na+, K+, Cl- and total bile acid, while good correlations (0.91 ≧ r ≧ 0.80) were recorded for albumin (r = 0.91). Bland-Altman difference plots also showed agreement (greater than 95% within Limits of Agreement) for glucose, total protein, albumin, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, blood urea nitrogen, creatinine, Na+, K+, Cl- and total bile acid between AmiShield and the reference instruments. However, aspartate aminotransferase and phosphorus exhibited higher outliers, implying potential problems associated with matrix interferences such as lipemic samples, which warrant further study. This study demonstrates that the AmiShield compares favourably with standard reference instruments, and the new device generated data of high quality for most analytes in clinical canine and feline samples. The capability of reliably measuring multi-category analytes in one device using minute amounts (170 μl) of whole blood and short turn-around times (< 15 min) underlines the high potential of the device as a good alternative in-house diagnostic application. Keywords: point-of-care, multi-analytes, whole blood Journal: Veterinární medicína Pages: 342-350 Volume: 62 Issue: 6 Year: 2017 DOI: 10.17221/109/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/109/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201706-0006.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:6:id:109-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D.H. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Korea Author-Name: H.J. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Korea Author-Name: E.J. Choi Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Korea Author-Name: K.W. Sah Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Korea Author-Name: H.S. Lee Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Korea Author-Name: K.B. Kwon Author-Workplace-Name: Yeeun Veterinary Hospital, Seoul, Korea Author-Name: H.Y. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Korea Author-Name: S.H. Do Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Korea Title: Extranodal marginal zone B-cell lymphomas of the bilateral third eyelids in a dog Abstract: Extranodal marginal zone B-cell type lymphoma of mucosa-associated lymphoid tissue (MALT) in dogs has similar properties to human low-grade B-cell lymphoma. Both are characterised by a relatively low mitotic rate and a slow manifestation of clinical signs. Primary MALT lymphoma of the third eyelid in canines is very rare. In this case report we describe bilateral MALT lymphoma in a 21-month-old miniature poodle. Histological analysis indicated that the masses were mainly composed of lymphoid cells and lymphoepithelial lesions, a typical feature of MALT lymphoma. Immunohistochemical analysis revealed that the neoplastic cells were positive for CD79α, but negative for CD3. We believe that this case report will facilitate the diagnosis, treatment, and prognosis of canine MALT lymphoma of the third eyelid. Keywords: immunohistochemistry, nuclear atypia, lymphoid cell, polycyctic lesion, ocular adnexal tumor, canine Journal: Veterinární medicína Pages: 351-355 Volume: 62 Issue: 6 Year: 2017 DOI: 10.17221/126/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/126/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201706-0007.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:6:id:126-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: G. Lanteri Author-Workplace-Name: Department of Veterinary Sciences, University of Messina, Italy Author-Name: G. Di Caro Author-Workplace-Name: Department of Veterinary Sciences, University of Messina, Italy Author-Name: M.T. Capucchio Author-Workplace-Name: Department of Veterinary Sciences, University of Torino, Grugliasco, Italy Author-Name: G. Gaglio Author-Workplace-Name: Department of Veterinary Sciences, University of Messina, Italy Author-Name: V. Reina Author-Workplace-Name: Veterinary practitioner Author-Name: C. Lo Giudice Author-Workplace-Name: Veterinary practitioner Author-Name: S. Zanet Author-Workplace-Name: Department of Veterinary Sciences, University of Torino, Grugliasco, Italy Author-Name: F. Marino Author-Workplace-Name: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Italy Title: Mesocestoidosis and multivisceral tetrathyridiosis in a European cat Abstract: This report describes the clinical, parasitological and pathological findings in a 6-year-old intact female European cat with thoracic and peritoneal tetrathyridiosis, characterized by genital involvement. Physical examination and X-ray evaluation revealed laboured breathing and several pulmonary nodules suggestive of cancer. However, necropsy demonstrated a parasitic aetiology of the disease. Histologically, multifocal granulomas were detected in the lungs, uterus and ovary. Parasitological examination permitted identification of the intestinal parasites as Mesocestoides lineatus, which was later confirmed by molecular examination. The larval forms in the peritoneal and chest cavity were identified as the second stage of the Mesocestoides sp. cestode named Tetrathyridia spp. The chronic injuries observed and the rapid course of the disease from the onset of the symptoms until death suggested a long period without clinical signs and indicate that overt disease can potentially be triggered by a failure of the immune system. The observed oophoritis and metritis identify tetrathyridiosis as a possible differential diagnosis in genital dysfunction. Keywords: feline, tetrathyridiosis, necropsy, histopathology, PCR Journal: Veterinární medicína Pages: 356-362 Volume: 62 Issue: 6 Year: 2017 DOI: 10.17221/6/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201706-0008.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:6:id:6-2017-VETMED