Template-Type: ReDIF-Article 1.0 Author-Name: L. Mesarcova Author-Name: J. Kottferova Author-Workplace-Name: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: L. Skurkova Author-Workplace-Name: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: L. Leskova Author-Workplace-Name: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: N. Kmecova Author-Workplace-Name: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Title: Analysis of cortisol in dog hair - a potential biomarker of chronic stress: a review Abstract: Cortisol, which is produced in the adrenal glands, is an endogenous glucocorticoid hormone that delivers its hormonal message to cells by acting on glucocorticoid receptors. It is one of the main stress hormones responsible for stress responses in animals and humans, and its overproduction is characteristic of certain diseases. While acute stress disorder can be evaluated by means of measuring the cortisol concentration in blood and urine, chronic stress disorder can be detected by monitoring the cortisol concentration in fur or hair. Hair collection is simple, inexpensive and non-invasive, and can be performed easily and rapidly; thus, it appears to be a suitable method for determining the level of stress in dogs from shelters, abused dogs or dogs involved in different types of animal interactions. Since it is a relatively new method, monitoring cortisol in hair or fur requires further research in order to definitively prove its efficacy, and possibly to determine reference range values for different breeds of dogs. Keywords: glucocorticoids, lifestyle, stress response, canine, coat colour, welfare Journal: Veterinární medicína Pages: 363-376 Volume: 62 Issue: 7 Year: 2017 DOI: 10.17221/19/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/19/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201707-0001.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:7:id:19-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Martins Author-Workplace-Name: Centre for Research and Technology of Agro-Environmental and Biological Sciences, University of Tras-os-Montes e Alto Douro, Vila Real, Portugal Author-Name: B. Colaço Author-Workplace-Name: Centre for Research and Technology of Agro-Environmental and Biological Sciences, University of Tras-os-Montes e Alto Douro, Vila Real, Portugal Author-Workplace-Name: Department of Animal Science; University of Tras-os-Montes e Alto Douro, Vila Real, Portugal Author-Name: S. Alves-Pimenta Author-Workplace-Name: Centre for Research and Technology of Agro-Environmental and Biological Sciences, University of Tras-os-Montes e Alto Douro, Vila Real, Portugal Author-Workplace-Name: Department of Animal Science; University of Tras-os-Montes e Alto Douro, Vila Real, Portugal Author-Name: A. Ferreira Author-Workplace-Name: Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal Author-Name: M. Ginja Author-Workplace-Name: Centre for Research and Technology of Agro-Environmental and Biological Sciences, University of Tras-os-Montes e Alto Douro, Vila Real, Portugal Author-Workplace-Name: Department of Veterinary Science, University of Tras-os-Montes e Alto Douro, Vila Real, Portugal Title: Effects of pelvis rotation on the projected radiographic position of the femoral head in relationship to the acetabulum Abstract: The aim of this study was to describe the influence of pelvis rotation on the projected radiographic position of the femoral head in relationship to the acetabulum in the standard ventrodorsal hip extended view, i.e. Norberg angle, pelvic thickness at the level of the cranial effective acetabular rim, femoral head subluxation index, femoral head subluxation category and femoral head-acetabular distance. Twelve adult dogs of large and giant breeds were radiographed in standard hip extended view and with 2°, 4° and 6° of rotation on the right and left sides. Norberg angle, pelvic thickness, subluxation index, subluxation category and femoral head-acetabular distance were measured on radiographs and analysed comparing a normal position with positions of different degrees of rotation for the dependent and non-dependent side. All the studied parameters showed adequate repeatability. Norberg angle and femoral head-acetabular distance did not show significant mean differences in all degrees of rotated pelvis (P > 0.05). The pelvic thickness showed an increase with the degree of rotation on the dependent side (P < 0.05) and a slight decrease on the non-dependent side (P > 0.05). The subluxation index and subluxation category showed an increase with the degree of rotation on the dependent side and a decrease on the non-dependent side (P < 0.05). As the subluxation index and subluxation category are parameters used in the classification of the main international hip dysplasia scoring systems, pelvic rotation can impair the final hip score, especially in scoring systems that are based on the worst joint. Keywords: femoral head-acetabular distance, Norberg angle, pelvic thickness, subluxation categories, subluxation index Journal: Veterinární medicína Pages: 377-385 Volume: 62 Issue: 7 Year: 2017 DOI: 10.17221/127/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/127/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201707-0002.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:7:id:127-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Lew-Kojrys Author-Name: E. Mikulska-Skupien Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Snarska Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: W. Krystkiewicz Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Pomianowski Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Title: Evaluation of clinical signs and causes of lower urinary tract disease in Polish cats Abstract: This paper describes the results of a retrospective study performed on 385 cats with feline lower urinary tract disease. The study was conducted to obtain epidemiological data and to evaluate clinical symptoms and the results of laboratory tests in a population of Polish cats with symptoms of lower urinary tract disease. The analysed population comprised feline patients of the veterinary clinic at the University of Warmia and Mazury in Olsztyn who had not been treated prior to admission. Medical history was obtained for all patients. Urine samples were collected mostly, but not solely, by cystocentesis. Feline idiopathic cystitis was diagnosed in most cats (60.7%), while urinary tract infections were noted in only 7.8% of patients. Urethral obstruction caused by mucus plugs was observed in 17.4% of animals. Urolithiasis was observed in 13% of cats, 5% of whom were also diagnosed with urinary tract infections. Hyperplastic changes were identified in only 1% of the studied population. In 59% of cats, feline lower urinary tract disease was accompanied by urethral obstruction. Cats with feline idiopathic cystitis were the youngest animals in the analysed population, and the risk of urinary tract infections and neoplasia increased with age. Our results, obtained over a period of six years in a Polish feline population, show that sex, neutering, age, living conditions and diet influence the type of urinary tract disease, data which are consistent with those obtained in other countries. Keywords: feline lower urinary tract disease, feline idiopathic cystitis, urethral obstruction, bacterial cystitis Journal: Veterinární medicína Pages: 386-393 Volume: 62 Issue: 7 Year: 2017 DOI: 10.17221/170/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/170/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201707-0003.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:7:id:170-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J.D. Hernandez Author-Workplace-Name: Veterinary Faculty, University of Las Palmas de Gran Canaria, Las Palmas, Spain Author-Workplace-Name: Serviexotic, Las Palmas, Spain Author-Name: P. Castro Author-Workplace-Name: Veterinary Faculty, University of Las Palmas de Gran Canaria, Las Palmas, Spain Author-Name: P. Saavedra Author-Workplace-Name: Department of Mathematics, University of Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Spain Author-Name: P. Ramirez Author-Workplace-Name: Serviexotic, Las Palmas, Spain Author-Name: J. Oros Author-Workplace-Name: Veterinary Faculty, University of Las Palmas de Gran Canaria, Las Palmas, Spain Title: Seasonal variations in haematological parameters in yellow-bellied slider turtles (Trachemys scripta scripta) Abstract: Yellow-bellied slider turtles (Trachemys scripta scripta) are increasingly being used as animal models for experimental purposes. The aim of this work was to generate some seasonal haematological information for captive yellow-bellied slider turtles as a useful and complementary tool for research trials. Blood samples from 30 healthy adult yellow-bellied turtles (13 males and 17 females) were obtained in winter and summer, and complete blood counts were performed. Within each season, the medians for males and females were compared using the Wilcoxon test for independent data. Likewise, the medians for both seasons were compared by means of the Wilcoxon test for paired data. All P-values were corrected using the Bonferroni multiple comparisons procedure. The concordance of the two methods used to determine the thrombocyte count (haemocytometer and smear estimation) was evaluated using intraclass correlation coefficients. The effects of sex were not statistically significant in either season (Bonferroni correction). However, values of heterophils, lymphocytes, basophils, and thrombocytes (haemocytometer method), when compared between seasons, exhibited differences that remained statistically significant after Bonferroni correction. Whereas heterophils were the most numerous leukocytes in summer for both sexes, basophils were the most numerous leukocytes in winter. In addition, lymphocyte percentages were also higher in winter for both sexes. Smear thrombocyte estimations did not show any significant differences between sexes and between seasons. However, when using the haemocytometer method, summer values were significantly higher than winter ones. The concordance between the two methods for measuring the thrombocyte count was not statistically significant in winter, but there was significant concordance in summer. Although the two techniques can be complementary, we recommend smear estimation as an easier and more reliable method. The haematological values obtained in this study may be useful as a complementary profile for researchers carrying out experimental procedures with this turtle subspecies. Keywords: haematology, reptile, blood, chelonian Journal: Veterinární medicína Pages: 394-400 Volume: 62 Issue: 7 Year: 2017 DOI: 10.17221/159/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/159/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201707-0004.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:7:id:159-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Fazio Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: V. Ferrantelli Author-Workplace-Name: Experimental Zooprophylaxy Institute of Sicily "A. Mirri", Palermo, Italy Author-Name: C. Saoca Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Author-Name: G. Giangrosso Author-Workplace-Name: Experimental Zooprophylaxy Institute of Sicily "A. Mirri", Palermo, Italy Author-Name: G. Piccione Author-Workplace-Name: Department of Veterinary Science, University of Messina, Messina, Italy Title: Stability of haematological parameters in stored blood samples of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) Abstract: The aim of this study was to investigate the influence of storage time at +4 °C on haematological indicators in rainbow trout Oncorhynchus mykiss (Walbaum, 1792). For this purpose, 60 Italian trout were evaluated and red blood cells, white blood cells, thrombocyte count, haematocrit, haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were determined. After blood collection (T0), all samples were immediately analysed using an automatic method to assess the haematological parameters and then divided into two different aliquots and stored at +4 °C. The first aliquot was refrigerated for 168 h (T1), the second one for 336 h (T2). Statistical analysis (one-way repeated-measures ANOVA) showed a significant effect of storage time (P < 0.05) on red blood cells, thrombocyte count, haematocrit, haemoglobin concentration, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. The obtained data expand our knowledge of haematological evaluation techniques and could contribute to the development of an appropriate method for haematological analysis that is suitable for the evaluation of the health status of wild and farmed fish. Keywords: fish farms, blood parameters, refrigeration, storage time Journal: Veterinární medicína Pages: 401-405 Volume: 62 Issue: 7 Year: 2017 DOI: 10.17221/51/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/51/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201707-0005.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:7:id:51-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: C. Serrano Author-Name: A. Ortilles Author-Workplace-Name: University of Zaragoza, Zaragoza, Spain Author-Name: A. Diaz Author-Workplace-Name: University of Zaragoza, Zaragoza, Spain Author-Name: A. Whyte Author-Workplace-Name: University of Zaragoza, Zaragoza, Spain Title: Corticoids as a treatment to control complications arising after reconstructive surgery of the skin: a case report Abstract: Corticosteroids are not indicated in wound healing, but the intralesional and topical administration of these agents has been described to control tissue overgrowth in humans. The aim of the present study was to assess the use of topical corticosteroids to control pre- and post-surgical tissue overgrowth in a 5-year-old male Belgian shepherd weighing 33.5 kg. We report successful use of topical administration of corticosteroids to treat granulation tissue overgrowth before and after skin grafting. Corticosteroids are a safe and easy way to treat this complication and can be used to prepare a healthy granulation tissue bed before performing surgical procedures to cover large defects in dogs. Keywords: corticosteroids, dog, granulation tissue, overgrowth, skin grafting Journal: Veterinární medicína Pages: 406-409 Volume: 62 Issue: 7 Year: 2017 DOI: 10.17221/154/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/154/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201707-0006.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:7:id:154-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Bzdil Author-Workplace-Name: Department of Special Microbiology, State Veterinary Institute Olomouc, Olomouc, Czech Republic Author-Name: O. Holy Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic Author-Name: J. Toporcak Author-Workplace-Name: Clinic of Birds, Exotic and Free Living Animals, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Title: Salmonella enterica subspecies enterica serovar Paratyphi B as a disease-causing agent in reptiles in the Czech Republic Abstract: The aim of this study was to describe two case studies of reptile disease, in which the primary pathogen was apparently Salmonella enterica subspecies enterica serovar Paratyphi B. Pathological examinations, sampling from different organs and cloacal swabs was performed on a dead savannah monitor (Varanus exanthematicus) and a sick green tree python (Chondropython viridis). This material was subjected to culture examination, including selective enrichment using standard methods. Typing was performed using MALDI-TOF and strains were also serologically typified. The utilisation of d-tartrate was confirmed biochemically and also using PCR. Antibiotic susceptibility was determined by the standard disc diffusion method using Mueller-Hinton agar without blood and antibiotic discs. In both cases the detected Salmonella enterica subspecies enterica serovar Paratyphi B was positive for d-tartrate and exquisitely susceptible to chloramphenicol, tetracycline, ampicillin, amoxycillin/clavulanic acid, gentamicin, ceftazidime, enrofloxacin and piperacillin. Keywords: paratyphoid, Varanus exanthematicus, Chondropython viridis, d-tartrate, treatment Journal: Veterinární medicína Pages: 410-415 Volume: 62 Issue: 7 Year: 2017 DOI: 10.17221/174/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/174/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201707-0007.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:7:id:174-2016-VETMED