Template-Type: ReDIF-Article 1.0 Author-Name: J. Siugzdaite Author-Workplace-Name: Lithuanian University of Health Science, Veterinary Academy, Kaunas, Lithuania Author-Name: A. Gabinaitiene Title: Methicillin-resistant coagulase-negative staphylococci in healthy dogs Abstract: The objective of this study was to evaluate the prevalence of coagulase-negative staphylococci in healthy dogs and to determine whether methicillin-resistant staphylococci expressed the mecA gene. Nasal and rectal swab samples were taken from 50 clinically healthy dogs. The prevalence of coagulase-negative staphylococci was evaluated according to phenotypic properties. The agar diffusion method was applied to evaluate antimicrobial resistance and the prevalence of methicillin resistance was determined using PCR analysing the mecA gene. A total of 59 coagulase-negative staphylococcus strains were isolated from the nostrils and rectums of 37 (74%) clinically healthy dogs. The prevalence of coagulase-negative staphylococci in female dogs was significantly higher compared with male dogs (P < 0.05). The results of antimicrobial susceptibility testing showed that 6.7% of the strains were resistant to oxacillin, 23.7% were resistant to penicillin, 22% to ampicillin and 16.9% to erythromycin. The mecA PCR revealed one oxacillin-sensitive and four oxacillin-resistant coagulase-negative staphylococci strains to be mecA carriers. Staphylococcus sciuri (60%) and Staphylococcus warneri (20%) were the most prevalent species among methicillin-resistant coagulase negative staphylococci. High antimicrobial resistance rates for these bacteria were observed against penicillin (100%), ampicillin (100%), oxacillin (80%), erythromycin (80%) and gentamicin (60%). All strains were susceptible to vancomycin and enrofloxacin. It is assumed that methicillin-resistance genes evolved in coagulase-negative staphylococcus and were then horizontally transferred among staphylococci. Keywords: Staphylococcus sciuri, antimicrobial resistant, mecA, age, nasal, rectal Journal: Veterinární medicína Pages: 479-487 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/96/2015-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/96/2015-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201709-0001.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:9:id:96-2015-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Titkova Author-Name: M. Fialkovicova Author-Workplace-Name: Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: M. Karasova Author-Workplace-Name: Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: J. Hajurka Author-Workplace-Name: Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Title: Puppy Apgar scores after vaginal delivery and caesarean section Abstract: The aim of this study was to evaluate the vital signs of newborn puppies using the modified Apgar score after spontaneous delivery and caesarean section. The survival of puppies was monitored for up to 24 hours after birth. The experiment consisted of ten deliveries of bitches with dystocia terminated by caesarean section (C-section group) and ten bitches with spontaneous vaginal delivery (SVD group). Vital signs of neonates were evaluated using a modified method of the Apgar score 5, 10 and 15 min after birth. Five evaluation criteria (colour of mucous membranes, reflex irritability, motility, heart rate and respiration) were used with puppies classified into three scoring groups: "severe distress" (0-3 points), "moderate distress" (4-6 points) and "no distress" (7-10 points). In the SVD group, 57 puppies were evaluated; 98.2% of puppies were born alive, all survived their first 24 hours of life. At the time of birth, 1.8% of puppies were dead (1/57). The Apgar scores of all foetuses were in the "no distress" category. In the C-section group (n = 37), 13.5% of puppies (5/37) were stillborn, two puppies (6.3%) died within 24 hours of delivery. The "no distress" group included 51.4% (19/37) of puppies, while 5.4% (2/37) of puppies were classified into "moderate distress" and 43.2% of puppies (16/37; P < 0.001 at 5 min, P < 0.005 at 10 min and P < 0.005 at 15 min after delivery) were classified into "severe distress". These results confirm the influence of type of delivery on the Apgar score immediately after parturition and on the short-term survival rate of puppies after parturition. Keywords: dog, dystocia, eutocia, neonatal distress, periparturient mortality of puppies Journal: Veterinární medicína Pages: 488-492 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/158/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/158/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201709-0002.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:9:id:158-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O. Bado Author-Name: M. Dlouha Author-Workplace-Name: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: E. Kolmanova Author-Workplace-Name: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Frydrych Author-Workplace-Name: Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Influence of new ultra-short-acting β-blockers on selected physiological indicators in laboratory rats Abstract: High rates of cardiovascular mortality have long been a serious problem in all European countries. Despite advancements in health care the situation is not improving fast enough. In the last decades, no new ultra-short-acting β-blockers have been registered in the European Union except for esmolol and landionol. In this study, eight newly-synthesised ultra-short-acting β-blockers were tested. These β-blockers contain an ester functional group which can be easily cleaved by plasma or cytoplasmic esterases. The substances were prepared in the Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno. Systolic blood pressure, heart rate and the interval of the QRS complex were evaluated using normotensive laboratory Wistar rats. The tested compounds were administered intravenously into the vena jugularis during general anaesthesia. The arteria carotis was exposed and cannulated to a Universal Perfusion System Basic Unit (UPSBU) of type Uniper UP-100. The universal perfusion system for isolated organs was capable of measuring and transducing actual values of blood pressure. ECG records were made using the ECG SEIVA - Praktik Veterinary. A series of substances named 2FC2a, 2FC2b, 2FC2c, 2FC2d, and another series with substances named 2FT2a, 2FT2b, 2FT2c, 2FT2d were tested at a dose of 3 mg/kg. Results were statistically compared to placebo. The best results were obtained for propyl and butyl derivatives with the highest lipophilicity. These acted as the best blood pressure reducers immediately after their administration. None of the compounds notably affected the heart rate. Statistical data show that carbamate substitution considerably prolongated the duration of the QRS complex as compared to placebo or etheric substitution. The carbamate substitution caused a pronounced arrhythmogenic effect. Thus, we could confirm the short-term hypotensive effect of the compounds. We observed an effect on the electrical conduction system of the heart while no effects were observed on heart rate. Our study contributes to better describing potential new ultra-short-acting β-blockers and facilitates selection for further testing. Keywords: ultra-short-acting β -blocker, laboratory rat, systolic blood pressure, heart rate, QRS complex Journal: Veterinární medicína Pages: 493-507 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/65/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/65/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201709-0003.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:9:id:65-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Schmidt-Ukaj Author-Workplace-Name: Service for Birds and Reptiles, Small Animal Internal Medicine, Vetmeduni Vienna, Vienna, Austria Author-Name: M. Hochleithner Author-Workplace-Name: Veterinary Hospital Strebersdorf, Vienna, Austria Author-Name: B. Richter Author-Workplace-Name: Institute of Pathology and Forensic Veterinary Medicine, Vetmeduni Vienna, Vienna, Austria Author-Name: C. Hochleithner Author-Workplace-Name: Veterinary Hospital Strebersdorf, Vienna, Austria Author-Name: D. Brandstetter Author-Workplace-Name: Veterinary Hospital Strebersdorf, Vienna, Austria Author-Name: Z. Knotek Author-Workplace-Name: Avian and Exotic Animal Clinic, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Title: A survey of diseases in captive bearded dragons: a retrospective study of 529 patients Abstract: The aim of this clinical retrospective study was to analyse the prevalence of common disorders in 529 captive bearded dragons that were presented to three exotic animal clinics in Central Europe (Austria and Czech Republic) over a period of three years. A diagnosis was made on the basis of the presenting clinical signs and physical examination in 30.8% of the cases, whereas various additional diagnostic tests were performed in the other cases (69.2%). These included diagnostic imaging (radiography, ultrasound and computed tomography), examination of faecal samples for the presence of parasites, a blood-profile analysis, histological, as well as bacteriological and mycological examinations and necropsy. Gastrointestinal diseases (42.67%) like endoparasitism, constipation, sand ingestion, tympany and meteorism were the most common disorders. In 51.92% of the cases of constipation, endoparasites were present, whereas in 38.46% of the cases of constipation, metabolic bone diseases and imbalances in calcium and phosphorus levels were detected. Most of the analysed faecal samples (83.27%) were positive for the presence of endoparasites (48.7% pinworms, 25.39% coccidians and 16.06% flagellates). Dermatological problems (22.4%) contained skin tumours, which were observed in adult lizards (age range from three to eight years) and included spindle cell tumour on the eyelid, as well as spindle cell sarcoma, squamous cell carcinoma and melanoma on the trunk. Osteodystrophy, limb fractures and various types of necrosis on the limbs and tail were frequently observed musculoskeletal disorders (18.93%). Over half of all animals (57.14%) that underwent a blood test, showed a deviation in the calcium-phosphorus ratio, 63.98% showed hypocalcaemia and 26.71% hyperphosphataemia. Diseases of the urogenital system (9.47%) included renal diseases and dystocia. Neoplastic diseases besides skin tumours included two cases of leukaemia in 4-year-old bearded dragons, one of these with lymphoma in kidneys, lungs and liver and one ganglioneuroma in the body cavity of a bearded dragon of unknown age. Orthopaedic surgeries were most frequently performed to amputate the limbs or tail, whereas soft-tissue surgeries most often included the treatment of skin wounds and correction of cloacal prolapse, in addition to ovariectomy, salpingotomy or salpingectomy in female bearded dragons. Similar to the situation in Australia and the USA, infectious as well as non-infectious diseases are common in captive bearded dragons in Central Europe. Due to the high occurrence of endoparasitism, skin diseases and metabolic bone diseases in this present study, regular veterinary controls in bearded dragons including parasitological faecal examinations and optimisation of feeding and housing are necessary to improve the standard of health of bearded dragons kept as pet lizards in Europe. Keywords: agamid lizards, captive reptiles, infectious diseases, metabolic diseases Journal: Veterinární medicína Pages: 508-515 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/162/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/162/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201709-0004.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:9:id:162-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: T.S. Hwang Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: D.I. Jung Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: J.H. Kim Author-Workplace-Name: College of Veterinary Medicine, Jeju National University, Jeju, Republic of Korea Author-Name: S.C. Yeon Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: H.C. Lee Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Title: Non-communicating small intestinal duplication in a dog: a case report Abstract: Enteric duplication is rare in dogs. Here, we report the rarest form of duplication in which two segments are parallel and share a wall for most of their lengths. A nine-year-old spayed female Yorkshire terrier was referred to the Veterinary Medical Teaching Hospital at Gyeongsang National University due to anorexia and diarrhoea. Physical examination, haematological examination, radiography, and ultrasonography were performed. On physical examination, dry, pale mucous membrane was identified. Moderate anaemia with decreased packed cell volume was detected in complete blood count. Serum urea nitrogen and creatinine levels were mildly increased. Radiographic images revealed no significant findings. On ultrasonographic examination, a multi-layered appearance of a focal small intestinal segment was identified in the left mid abdomen. Following the lesion, it was divided into two small intestinal segments. Based on imaging findings, intussusceptions or enteric duplication were suspected. To resect the abnormal small intestinal segment, enterectomy was performed. Follow-up was not performed because the patient expired during the postoperative recovery time. The histopathological diagnosis was non-communicating small intestinal duplication. Non-communicating intestinal duplication is related to embryologic abnormalities and is usually concurrent with other anomalies such as vertebral malformations and urogenital duplications. However, this case had no other anomalies associated with the malformation of the intestine. Keywords: intestine, enteric duplication, ultrasonography malformation, surgery Journal: Veterinární medicína Pages: 516-521 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/73/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/73/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201709-0005.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:9:id:73-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Sung Author-Workplace-Name: Chonbuk National University, Iksan, Republic of Korea Author-Name: S. Lim Author-Workplace-Name: Chonbuk National University, Iksan, Republic of Korea Author-Name: H. Oh Author-Workplace-Name: Chonbuk National University, Iksan, Republic of Korea Author-Name: K. Kim Author-Workplace-Name: Chonbuk National University, Iksan, Republic of Korea Author-Name: Y. Choi Author-Workplace-Name: Chonbuk National University, Iksan, Republic of Korea Author-Name: K. Lee Title: Atypical radiographic features of multiple myeloma in a dog: a case report Abstract: A 12-year-old castrated male miniature pinscher dog presented with right forelimb lameness. An expansile osteolytic lesion of the right humerus was conspicuous on right forelimb radiographs. Thoracic and abdominal radiographs showed multiple lytic bone lesions on the right scapula, ribs, vertebrae, femurs and pelvis. Additionally, an expansile osteolytic lesion was observed in two thoracic vertebrae and two ribs. Many plasma cells were identified on fine-needle aspiration of the right humerus expansile osteolytic lesion, and monoclonal gammopathy consistent with multiple myeloma was confirmed on serum protein electrophoresis. The expansile osteolytic lesion, an extraordinary radiographic feature of multiple myeloma in dogs, should be considered as a possible radiographic finding of multiple myeloma. Keywords: plasma cell myeloma, radiography, bone, expansile lesion Journal: Veterinární medicína Pages: 522-526 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/140/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/140/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201709-0006.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:9:id:140-2016-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J.Y. Kong Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: H.K. Kim Author-Workplace-Name: Dr. Kim Animal Hospital, Seoul, Republic of Korea Author-Name: H.J. Lee Author-Workplace-Name: Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: S.C. Yeon Author-Workplace-Name: Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Name: J.K. Park Author-Workplace-Name: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: K.S. Jeong Author-Workplace-Name: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: I.H. Hong Author-Workplace-Name: College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Author-Workplace-Name: Institute of Animal Medicine, Gyeongsang National University, Jinju, Republic of Korea Title: Embryonal rhabdomyosarcoma in a Siberian chipmunk (Tamias sibiricus): a case report Abstract: A 2-year-old female Siberian chipmunk (Tamias sibiricus) was presented to the veterinary clinic for swelling, pain and lameness of the left rear leg. Radiologically, an invasive tumour around the distal femur was suspected, and the leg was surgically amputated and submitted for histopathological diagnosis. Microscopically, the mass was densely packed with multinucleated strap cells that had round-to-oval, or elongated nuclei with prominent nucleoli. These neoplastic cells occasionally formed myotubes with cross-striations and were immunohistochemically positive for muscle markers including desmin and myogenin. Consequently, embryonal rhabdomyosaroma myotubular variant of the leg with metastasis to the femur was diagnosed. Spontaneous rhabdomyosaromas are rare tumours in animals and humans, and this is the first report of its occurrence in a Siberian chipmunk. Keywords: myogenin, PTAH stain, rodents, skeletal muscle, squirrel Journal: Veterinární medicína Pages: 527-531 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/173/2016-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/173/2016-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201709-0007.txt Handle: RePEc:caa:jnlvet:v:62:y:2017:i:9:id:173-2016-VETMED