Template-Type: ReDIF-Article 1.0 Author-Name: K. Machackova Author-Name: M. Boselova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: I. Vanova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: Z. Drabkova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: J. Doubek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Title: Evaluation of kaolin-activated thromboelastography and sample stability in healthy horses Abstract: Thromboelastography is an accurate alternative to routine coagulation testing for the monitoring of haemostasis. However, its use in equine medicine is limited not only by the lack of reference interval values for kaolin-activated citrated samples, but also by the limited accessibility of the test for field practitioners within the 2-hour storage time recommended by the manufacturer. To address this issue, we here evaluated kaolin-activated thromboelastography using a TEG® 5000 Thrombelastograph® Hemostasis System in 36 healthy horses, and sample stability was evaluated at four timepoints post collection in seven horses. Reference values were established as follows: reaction-time 5.0-16.0 min, K-time (period in which the clot strength reaches 20 mm of amplitude) 1.1-5.2 min, α-angle (speed of fibrin cross-linking) 36.5-79.0°, maximal amplitude 44.5-69.7 mm, fibrinolysis 30 minutes after maximal amplitude was reached 0.0-2.8%. During storage, the trends in the changes of values were similar for most parameters, and values remained mostly within the reference intervals. Thromboelastography is thus useful in defining thrombohaemorrhagic complications in horses but can be sensitive to preanalytical factors and storage. Keywords: haemostasis, TEG, physiological values, coagulation, stability Journal: Veterinární medicína Pages: 203-209 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/16/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/16/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201805-0001.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:5:id:16-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Pidsudko Author-Workplace-Name: University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: J. Kaleczyc Author-Workplace-Name: University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: J. Zmudzki Author-Workplace-Name: National Veterinary Research Institute, Pulawy, Poland Author-Name: W. Sienkiewicz Author-Workplace-Name: University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: M. Zalecki Author-Workplace-Name: University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: M. Klimczuk Author-Workplace-Name: University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: K. Wasowicz Author-Workplace-Name: University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Title: Changes in the tissue concentrations of several neuropeptides in porcine intestines and intestine-innervating ganglia in the course of porcine proliferative enteropathy Abstract: Inflammatory processes are associated with changes in the interplay of different pro- and anti-inflammatory factors, including neuropeptides, in tissue. This study was performed to investigate the influence of proliferative enteropathy on the concentration of several neuropeptides known to be involved in the regulation of the inflammatory process in porcine intestine and intestine-innervating ganglia. The concentration of galanin, vasoactive intestinal polypeptide, somatostatin, neuropeptide Y, substance P and calcitonin gene-related peptide were assayed with ELISA in the coeliac-superior mesenteric ganglion, inferior mesenteric ganglion, selected dorsal root ganglia, ileum and the descending colon in healthy and sick pigs. The concentrations of the studied neuropeptides were higher in sick animals. Statistically significant differences were found for coeliac-superior mesenteric ganglion (galanin, vasoactive intestinal polypeptide, somatostatin and neuropeptide Y), inferior mesenteric ganglion (galanin, somatostatin and neuropeptide Y), dorsal root ganglia (galanin, somatostatin, neuropeptide Y and calcitonin gene-related peptide), ileum (galanin and somatostatin) and the descending colon (galanin, somatostatin and neuropeptide Y). The data clearly show the influence of the inflammatory process on the concentration of some of the studied neuropeptides present in inflamed tissues and ganglia innervating the inflamed gut. These changes must be associated with the role the studied neuropeptides play in the inflammatory process. Keywords: adenomatosis, inflammation, gut, neuropeptides, pig Journal: Veterinární medicína Pages: 210-215 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/62/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/62/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201805-0002.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:5:id:62-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B. Gasser Author-Workplace-Name: School of Agrarian Sciences and Veterinary Medicine, University Estadual Paulista "Julio de Mesquita Filho", Jaboticabal, Brazil Author-Name: M.G.K. Rodriguez Author-Workplace-Name: School of Agrarian Sciences and Veterinary Medicine, University Estadual Paulista "Julio de Mesquita Filho", Jaboticabal, Brazil Author-Name: R.A.R. Uscategui Author-Workplace-Name: School of Agrarian Sciences and Veterinary Medicine, University Estadual Paulista "Julio de Mesquita Filho", Jaboticabal, Brazil Author-Name: P.A. Silva Author-Workplace-Name: School of Agrarian Sciences and Veterinary Medicine, University Estadual Paulista "Julio de Mesquita Filho", Jaboticabal, Brazil Author-Name: M.C. Maronezi Author-Workplace-Name: School of Agrarian Sciences and Veterinary Medicine, University Estadual Paulista "Julio de Mesquita Filho", Jaboticabal, Brazil Author-Name: L. Pavan Author-Workplace-Name: School of Agrarian Sciences and Veterinary Medicine, University Estadual Paulista "Julio de Mesquita Filho", Jaboticabal, Brazil Author-Name: M.A.R. Feliciano Author-Workplace-Name: School of Agrarian Sciences and Veterinary Medicine, University Estadual Paulista "Julio de Mesquita Filho", Jaboticabal, Brazil Author-Workplace-Name: Department of Veterinary Medicine, Federal University of the "Reconcavo da Bahia", Cruz das Almas, Bahia, Brazil Author-Name: W.R.R. Vicente Author-Workplace-Name: School of Agrarian Sciences and Veterinary Medicine, University Estadual Paulista "Julio de Mesquita Filho", Jaboticabal, Brazil Title: Ultrasonographic characteristics of benign mammary lesions in bitches Abstract: The aim of this study was to evaluate the applicability of B-mode, Doppler, acoustic radiation force impulse elastography and contrast-enhanced ultrasound exams in the differentiation of neoplastic and non-neoplastic benign mammary lesions in bitches. This research was conducted as a prospective secondary observational cohort (2014-2016) study, which included 36 mammary lesions, evaluated physically and by ultrasound (B-mode, Doppler, contrast-enhanced ultrasound and acoustic radiation force impulse) exams prior to mastectomy and histopathological classification as neoplastic or non-neoplastic lesions. All ultrasonographic parameters studied were compared between histopathological classifications using Fisher's or Student's tests and differences were considered significant when P < 0.05. Out of 36 benign mammary lesions evaluated, 25 were classified as neoplastic and 11 as non-neoplastic. The qualitative and quantitative parameters evaluated using the different ultrasound methods were not effective (P > 0.05) in differentiating between neoplastic and non-neoplastic mammary masses in bitches. Nevertheless, some B-mode variables, such as longitudinal length (P = 0.0292), width/length ratio (P = 0.0001) and width/height ratio (P = 0.0001) showed limited efficacy in the differentiation of mammary lesions types. In conclusion, ultrasonographic evaluation of benign canine mammary lesions did not allow differentiation between neoplastic and non-neoplastic tissues and only a few B-mode variables may aid in the prediction of specific tumour types. Keywords: Doppler, acoustic radiation force impulse, ARFI elastography, contrast-enhanced ultrasound, dogs, neoplastic Journal: Veterinární medicína Pages: 216-224 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/87/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/87/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201805-0003.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:5:id:87-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Adaszek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland Author-Name: P. Lyp Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland Author-Name: P. Poblocki Author-Workplace-Name: Veterinary Clinic, Kosakowo, Poland Author-Name: M. Skrzypczak Author-Workplace-Name: University School of Medicine, Lublin, Poland Author-Name: L. Mazurek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland Author-Name: S. Winiarczyk Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland Title: The first case of Babesia gibsoni infection in a dog in Poland Abstract: Canine babesiosis is a tickborne, protozoal, haemoparasitic disease that can cause varying degrees of haemolytic anaemia, splenomegaly, thrombocytopenia and fever. Babesia organisms are frequently classified as either large or small. All small Babesia infections were previously attributed to B. gibsoni, but molecular analysis and DNA sequencing have revealed that there are at least three small piroplasms which infect dogs. Correctly identifying the infectious agent is important for treatment planning and prognosis. In this report, the first case of Babesia gibsoni infection in a Polish dog is presented. Keywords: tickborne, molecular analysis, DNA sequencing, vector-borne diseases Journal: Veterinární medicína Pages: 225-228 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/155/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/155/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201805-0004.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:5:id:155-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Pospichal Author-Workplace-Name: Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: D. Pokorova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: T. Vesely Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: V. Piackova Author-Workplace-Name: Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Title: Susceptibility of the topmouth gudgeon (Pseudorasbora parva) to CyHV-3 under no-stress and stress conditions Abstract: Cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus, is the causative agent of the highly contagious koi herpesvirus disease, which is restricted to koi and common carp and causes significant losses in both fish stock. Some experimental investigations have shown that other cyprinid or non-cyprinid species may be asymptomatically susceptible to this virus and might play roles as potential carriers of CyHV-3 or might contribute to persistence of this virus in environment. Therefore, it seems important to verify not only the susceptibility of other cyprinid or non-cyprinid species, but also their ability to transmit CyHV-3 infection to susceptible species. Our previous investigation of the susceptibility of the topmouth gudgeon (Pseudorasbora parva) did not reveal the presence of CyHV-3 DNA in the tissues of this species after cohabitation with infected koi. Consequently, we changed the experimental conditions and applied two stress factors (removal of skin mucus and scaring) which would presumably mimic the stress most commonly encountered in the wild. Both experiments (without and with stress factors) consisted of primary and secondary challenges. In both the no-stress and stress experiments, the first challenge was focused only on testing the susceptibility of the topmouth gudgeon to the virus. With the secondary challenge, we investigated potential viral transmission from the topmouth gudgeon to healthy naive koi after exposure to stress factors. All fish (dead, surviving and sacrificed) were tested for the presence of CyHV-3 DNA using nested PCR (no-stress experiment) and real-time PCR (stress experiment). After the primary challenge of the no-stress experiment, PCR did not reveal the presence of CyHV-3 DNA in any specimen of cohabitated topmouth gudgeon, but all specimens of dead koi were CyHV-3 DNA-positive. PCR of fish tissues subjected to the secondary challenge did not show the transfer of virus to naive fish. After exposure to stress (removal of skin mucus), qPCR revealed four out of five samples (80%) of topmouth gudgeon to be positive for CyHV-3 DNA. Two out of five samples (40%) of topmouth gudgeon treated by scaring were found to be positive for the presence of viral DNA. Real-time PCR after the secondary challenge did not reveal any viral DNA positivity in specimens of topmouth gudgeon from groups previously exposed to stress. The stress experiments show that removal of skin mucus might potentially lead to susceptibility of topmouth gudgeon to CyHV-3 infection, but the transmission of the virus to koi carp was not observed. Keywords: resistance, transmission, viral disease, KHV, carrier Journal: Veterinární medicína Pages: 229-239 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/88/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/88/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201805-0005.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:5:id:88-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N. Butto Author-Workplace-Name: Faculty of Veterinary and Animal Science, University of Chile, Santiago de Chile, Chile Author-Name: C. Hamilton-West Author-Workplace-Name: Faculty of Veterinary and Animal Science, University of Chile, Santiago de Chile, Chile Author-Name: J.I. Arias Title: In vitro heat transfer from epoxy polymer and poly(methyl methacrylate) to fixation pins: recommendations to avoid tissue damage in free-form external skeletal fixation Abstract: External skeletal fixation has been used for the management of fractures of many types of bone. The use of polymeric free-form connecting bars in external fixators has become widely used in veterinary orthopaedics because of its versatile forms of frame construction and its relative low costs. Determining thermal-transfer to trans fixation pins during polymerisation of poly(methyl methacrylate) and epoxy putty polymers used for free-form external skeletal fixation connecting bars is important for avoiding temperatures of more than 47 °C, which would cause thermal soft tissue injury. Therefore, thermal transfer to trans fixation pins was measured in vitro during the polymerisation phase of these polymers. We used trocar-pointed pins of different diameters that punctured one wall of a connecting tube, resulting in the tip of the trocar-pointed pin reaching the centre of the tube. The FLUKE® VT02 infrared digital camera was then used to measure heat transfer to the pins at 1 or 2 cm from poly(methyl methacrylate) or epoxy putty. The polymerisation temperatures of these polymers yield a potentially dangerous level of heat for soft and hard tissue. This was observed in almost all the experimental conditions tested. On the other hand, epoxy putty transfer to the pins did not cause the temperature to reach 47 °C at any time or in any of the setups examined. Poly(methyl methacrylate) did reach more than 47 °C and remained at that level for more than 1 min at 1 cm from the polymer. This acrylate exhibited polymerisation temperatures higher than epoxy and its heat transfer to the pins was potentially dangerous if used at less than 1 cm from soft or hard tissue. Keywords: epoxy putty, bone necrosis, exothermic polymerisation, infrared digital image Journal: Veterinární medicína Pages: 240-247 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/107/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/107/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201805-0006.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:5:id:107-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Yaygingul Author-Name: N. Kilic Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Author-Name: B. Kibar Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Title: Surgical treatment of a mandibular symphyseal fracture in a calf using a continuous wire-loop technique: a case report Abstract: In this case report, we describe the clinical and radiological features of a calf mandibular symphyseal fracture and evaluate the treatment results. A 1.5-month-old male Holstein weighing 65 kg was presented to the Surgery Clinic, Faculty of Veterinary Medicine, Adnan Menderes University with a history of salivation and anorexia for three days. After clinical and radiographic assessments, a mandibular symphyseal fracture was determined. Stabilisation of the fracture region was achieved using cerclage wire with a continuous wire-loop technique under general anaesthesia. The cerclage wire was removed five weeks post-surgery. The calf was clinically healthy. Keywords: cow, bovine, lower jaw, cerclage wire Journal: Veterinární medicína Pages: 248-250 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/119/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/119/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201805-0007.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:5:id:119-2017-VETMED