Template-Type: ReDIF-Article 1.0 Author-Name: K. Kara Author-Workplace-Name: Veterinary Medicine Faculty, Erciyes University, Kayseri, Turkey Author-Name: S. Ozkaya Author-Workplace-Name: Agriculture Faculty, Suleyman Demirel University, Isparta, Turkey Author-Name: E. Baytok Author-Workplace-Name: Veterinary Medicine Faculty, Erciyes University, Kayseri, Turkey Author-Name: B.K. Guclu Author-Workplace-Name: Veterinary Medicine Faculty, Erciyes University, Kayseri, Turkey Author-Name: E. Aktug Author-Workplace-Name: Veterinary Medicine Faculty, Erciyes University, Kayseri, Turkey Author-Name: S. Erbas Author-Workplace-Name: Agriculture Faculty, Suleyman Demirel University, Isparta, Turkey Title: Effect of phenological stage on nutrient composition, in vitro fermentation and gas production kinetics of Plantago lanceolata herbage Abstract: This study was aimed at determining the nutrient composition and in vitro ruminal digestion values of Plantago lanceolata herbage in different phenological stages. The plant samples were gathered in the vegetative, flowering and early seed stages of the plant. The crude protein, diethyl ether extract, ash, non-fibre carbohydrates, and proanthocyanidins levels of the vegetative and flowering stages were higher than those of the early seed stage (P < 0.001). Structural carbohydrate levels (P < 0.05) were determined to have a higher value in the early seed stage. Glucose, fructose, Ca, K, Mg, P, Fe and Cu concentrations decreased as the plant matured (P < 0.001), but Na, Zn, and Mn concentrations increased (P < 0.05). The asymptote gas production, gas production rate (P < 0.001), total gas production at 24 h (P = 0.002), metabolic energy, net energy lactation and organic matter digestibility values and the number of Entodinium (P < 0.001) and total bacteria count (P = 0.026) of the flowering and vegetative stages were higher than those of the early seed stage. Methane produced by 0.2 g dry matter was similar in the three phenological stages (P = 0.078). The bound condensed tannins and saponin contents of plants and ammoniacal-N, number of total protozoa and pH value of rumen fluid were similar in the three different phenological stages (P > 0.05). The present study indicates that P. lanceolata in the vegetative and flowering stages has, owing to its chemical composition, energy content and digestibility, the potential to be used as a forage source for ruminants in areas affected by drought. Keywords: in vitro gas production, Plantaginaceae, ruminal microbiota, vegetative Journal: Veterinární medicína Pages: 251-260 Volume: 63 Issue: 6 Year: 2018 DOI: 10.17221/2/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201806-0001.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:6:id:2-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: W. Sienkiewicz Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Dudek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Chroszcz Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: M. Janeczek Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: J. Kaleczyc Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Title: Distribution and immunohistochemical properties of autonomic neurons supplying the ovine hip joint capsule Abstract: Combined retrograde tracing and double labelling immunohistochemistry were applied to study the distribution and chemical coding of autonomic neurons projecting to the ovine hip joint capsule. As revealed by retrograde tracing, fast blue-positive autonomic neurons supplying the lateral side of the hip joint capsule and the medial side of the hip joint capsule were located within the lumbar and sacral of the ipsilateral sympathetic chain ganglia and within the caudal mesenteric ganglion. Immunohistochemistry revealed that nearly all (sympathetic chain ganglia: 96% and caudal mesenteric ganglion: 98.8%) the neurons were adrenergic in nature (positive for dopamine β-hydroxylase). Many retrogradely labelled neurons also displayed immunoreactivity to neuropeptide Y (approximately 34% of fast blue-positive neurons within caudal mesenteric ganglion and sympathetic chain ganglia). Populations of Met-Enk+ (20%) and Leu-Enk+ (6%) neurons were present only in the sympathetic chain ganglia while within caudal mesenteric ganglion no enkephalinergic-labelled neurons were noted. Only a small population (2.2%) of hip joint capsule-projecting neurons were Gal-IR and they were observed only within the caudal mesenteric ganglion. No cholinergic neurons involved in the innervation of the hip joint capsule were found. However, fast blue-positive nerve cell bodies were surrounded by numerous cholinergic nerve fibres often forming basket-like formations. Single Gal+ nerve fibres were found in the intraganglionic connective tissue. Substance P-positive or calcitonin gene-related peptide-positive intraganglionic nerve terminals were very numerous and formed "baskets" surrounding fast blue-positive perikarya within sympathetic chain ganglias and caudal mesenteric ganglion. Keywords: sheep, hip joint capsule, tracing, autonomic neurons, immunohistochemistry Journal: Veterinární medicína Pages: 261-270 Volume: 63 Issue: 6 Year: 2018 DOI: 10.17221/61/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/61/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201806-0002.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:6:id:61-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Imani Rastabi Author-Name: A. Baniadam Author-Workplace-Name: Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Author-Name: A. Ronagh Author-Workplace-Name: Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Author-Name: A. Khajeh Author-Workplace-Name: Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Author-Name: M. Kamyabnia Author-Workplace-Name: Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Title: Comparison of intraocular pressure, tear production and cardiorespiratory variables before and after induction of anaesthesia with either propofol or ketofol in dogs premedicated with midazolam Abstract: The objective of the present study was to compare the effects of propofol and ketofol on intraocular pressure, tear production and cardiorespiratory variables in dogs premedicated with midazolam. Six castrated adult mixed-breed dogs were used in a cross-over design with a one-week interval. Twenty minutes after premedication with midazolam (0.2 mg/kg), animals were assigned randomly to two groups and received either propofol (6 mg/kg) or ketofol (3 mg/kg; 1 : 1 mg/ml ratio) treatments intravenously. Intraocular pressure, tear production, heart rate, respiratory rate, rectal temperature and direct mean arterial blood pressure were measured at base (before induction), and at 5, 10, 15, 20 and 30 min after induction of anaesthesia. Blood gas samples were obtained at base (before induction), and at 5, 15 and 30 min after administration of treatments. Intraocular pressure showed significantly higher values at 5 min after induction in ketofol compared with propofol (16.1 ± 4.5 mm Hg vs 8.2 ± 1.2 mm Hg, respectively). There were no significant changes in tear production in either group. Significantly higher heart rate and mean arterial blood pressure were detected in ketofol at several time points. Respiratory depression occurred in both groups with no significant differences between them. In conclusion, although ketofol improved heart rate and mean arterial blood pressure and did not elicit more pronounced respiratory depression than propofol, it resulted in significantly higher values of intraocular pressure at 5 min after administration in dogs. Despite the small number of dogs in this study, our results indicate that ketofol should not be recommended for ophthalmic surgical procedures in dogs. Appropriate oxygenation should be provided when propofol is used for ophthalmic surgeries. Keywords: heart rate, respiratory rate, rectal temperature, direct mean arterial blood pressure, blood gases Journal: Veterinární medicína Pages: 271-278 Volume: 63 Issue: 6 Year: 2018 DOI: 10.17221/18/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/18/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201806-0003.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:6:id:18-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.Y. Heo Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of Korea Author-Name: S.J. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of Korea Author-Name: N.S. Kim Title: Analgesic efficacy of meloxicam with or without a buprenorphine patch in cats after ovariohysterectomy Abstract: The purpose of this prospective double blind clinical study was to evaluate the analgesic efficacy of meloxicam with/without a buprenorphine patch for pain management after ovariohysterectomy in cats. Cats were randomly divided into two groups: ten cats were treated with meloxicam s.c. after ovariohysterectomy (Group A), and eight cats were treated with s.c. meloxicam and a 20 µg/h buprenorphine transdermal patch (Group B). For patch treatment, the cat's hair was clipped on the left side in the thoracic area. Pain scores were assessed at 0.5, 1, 2, 4, 6, 8, 24 and 30 h post-ovariohysterectomy extubation. To evaluate postoperative pain, 4A-VET pain scale and visual analogue scale pain scores were used. In addition, blood was collected from all cats to determine the cortisol levels at -2 h and at 0.5, 4, 6 and 24 h after extubation. The 4A-VET scores for Group B were significantly lower at 1, 4, 6, 8, 24 and 30 h than the scores for Group A. The visual analogue scale pain scores for Group B were significantly lower at 4, 6, 24 and 30 h than the scores for Group A. Serum cortisol concentrations were not significantly different between Groups A and B at any of the measured intervals. There was a significant positive correlation between postoperative visual analogue scale and 4A-VET pain scores in both groups. Our results should be subject to careful interpretation as the study was limited by its small sample size and by observer subjectivity. Keywords: cat, transdermal route, postoperative pain, 4A-VET pain scale, visual analogue scale, cortisol Journal: Veterinární medicína Pages: 279-286 Volume: 63 Issue: 6 Year: 2018 DOI: 10.17221/148/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/148/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201806-0004.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:6:id:148-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Pellattiero Author-Workplace-Name: Department of Animal Medicine, Production and Health (MAPS), University of Padua, Legnaro, Italy Author-Name: C.M. Tucciarone Author-Workplace-Name: Department of Animal Medicine, Production and Health (MAPS), University of Padua, Legnaro, Italy Author-Name: G. Franzo Author-Workplace-Name: Department of Animal Medicine, Production and Health (MAPS), University of Padua, Legnaro, Italy Author-Name: G. Berto Author-Workplace-Name: Ceva Salute Animale, Agrate Brianza, Italy Author-Name: K. Koutoulis Author-Workplace-Name: Ceva Sante Animale, Libourne, France Author-Name: A. Meini Author-Workplace-Name: Ceva Salute Animale, Agrate Brianza, Italy Author-Name: C. Zangrandi Author-Workplace-Name: Ceva Salute Animale, Agrate Brianza, Italy Author-Name: G. Ramon Author-Workplace-Name: Ceva Sante Animale, Libourne, France Author-Name: M. Drigo Author-Workplace-Name: Department of Animal Medicine, Production and Health (MAPS), University of Padua, Legnaro, Italy Author-Name: M. Cecchinato Author-Workplace-Name: Department of Animal Medicine, Production and Health (MAPS), University of Padua, Legnaro, Italy Title: Evaluation of unintended 1/96 infectious bronchitis vaccine transmission in broilers after direct contact with vaccinated ones Abstract: Infectious bronchitis virus is characterised by an extreme degree of variability which deeply affects the first-choice control strategies against the disease. Each country tends to adopt its own protocols and even vaccine producers themselves can also adopt different strategies in attempts to confront local epidemiological concerns. In the present study, we tested the potential environmental persistence, transmission ability and replication capability of a non-directly administrated vaccine strain at a hatchery and during transportation. To this purpose, we examined a single hatchery, where combined vaccination (Mass-like plus 793B-like strains) is commonly administered following the protectotype concept, whereas a single broiler flock receives only the Mass priming. Two groups of solely Mass-primed chicks were kept in contact with chicks vaccinated with both strains, during hatchery procedures and transportation, respectively. A regularly vaccinated control group was selected and all three were monitored by swab sampling until 11 days of age. Vaccine titres were quantified using vaccine-specific real-time RT-PCRs to check the kinetics of both strains. Mass titres were consistent among the groups, while the absence of the 1/96 vaccine strain in unvaccinated chicks confirmed the low risk of unintended vaccine transmission, which could complicate the diagnostic process and the epidemiological scenario. Keywords: infectious bronchitis virus, Mass-like strain, 793B-like strain, vaccine exposure, vaccine kinetics, vaccine spreading Journal: Veterinární medicína Pages: 287-291 Volume: 63 Issue: 6 Year: 2018 DOI: 10.17221/76/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/76/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201806-0005.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:6:id:76-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Balajty Author-Workplace-Name: Institute of Forensic and Public Veterinary Medicine and Economy, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: D. Takacova Author-Workplace-Name: Institute of Forensic and Public Veterinary Medicine and Economy, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: G. Ruffo Author-Workplace-Name: Department of Health, Animal Science and Food Safety - VESPA, University of the Studies of Milano, Milano, Italy Author-Name: P. Fossati Author-Workplace-Name: Department of Health, Animal Science and Food Safety - VESPA, University of the Studies of Milano, Milano, Italy Title: Differences between the Italian and Slovak legal systems with respect to animal protection Abstract: This paper provides a comparative analysis of the legal content of animal protection legislation in the Slovak Republic with that of Italian legislation. Firstly, we compare the legal systems in the Slovak Republic and Italy with respect to the animal protection laws, especially criminal law in both countries. This comparison of the two different legislations allows detection of identical or different characteristics in the area of animal protection, which could be used later as an inspiration for lawmakers in both countries. Keywords: animal protection, Italian legislation, Slovak legislation, criminal code, cruelty to animals Journal: Veterinární medicína Pages: 292-297 Volume: 63 Issue: 6 Year: 2018 DOI: 10.17221/4/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/4/2018-VETMED.html File-Format: text/html Handle: RePEc:caa:jnlvet:v:63:y:2018:i:6:id:4-2018-VETMED