Template-Type: ReDIF-Article 1.0 Author-Name: L. Bartosikova Author-Name: J. Necas Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Title: Epigallocatechin gallate: a review Abstract: Epigallocatechin gallate is the major component of the polyphenolic fraction of green tea and is responsible for most of the therapeutic benefits of green tea consumption. A number of preclinical in vivo and in vitro experiments as well as clinical trials have shown a wide range of biological and pharmacological properties of polyphenolic compounds such as anti-oxidative, antimicrobial, anti-allergic, anti-diabetic, anti-inflammatory, anti-cancer, chemoprotective, neuroprotective and immunomodulatory effects. Epigallocatechin gallate controls high blood pressure, decreases blood cholesterol and body fat and decreases the risk of osteoporotic fractures. Further research should be performed to monitor the pharmacological and clinical effects of green tea and to more clearly elucidate its mechanisms of action and the potential for its use in medicine. Keywords: epigallocatechin-3-O-gallate, pharmacokinetics, toxicity, biological activity Journal: Veterinární medicína Pages: 443-467 Volume: 63 Issue: 10 Year: 2018 DOI: 10.17221/31/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/31/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201810-0001.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:10:id:31-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.J. Ding Author-Workplace-Name: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Author-Name: Y.R. Luo Author-Workplace-Name: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Author-Name: S.T. Zhou Author-Workplace-Name: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Author-Name: C. Xie Author-Workplace-Name: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Author-Name: K. Wang Author-Workplace-Name: Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Shanghai, China Author-Name: Y.Y. Xie Author-Workplace-Name: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Author-Name: L. Cui Author-Workplace-Name: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Author-Name: X.G. Hua Author-Workplace-Name: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Author-Name: C.L. Yuan Author-Workplace-Name: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Author-Name: Y.J. Zhou Author-Workplace-Name: Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Shanghai, China Author-Name: Z.B. Yang Author-Workplace-Name: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China Title: Preparation and characterisation of monoclonal antibodies against the N protein of the SHpd/2012 strain of porcine epidemic diarrhoea virus Abstract: Porcine epidemic diarrhoea is caused by the porcine epidemic diarrhoea virus, and is a highly contagious disease which affects the intestines of new-born piglets resulting in intense diarrhoea. Historically, the virus has caused enormous economic losses in the pig industry. In particular, the emergence of new epidemic strains means there is a pressing need for prevention and control of the disease. Owing to the specificity of the monoclonal antibodies now available, study of the pathogenesis, immune mechanisms and new diagnostic methods can be performed. In this study, 13 strains of positive hybridoma cells were prepared by immunising mice with purified whole porcine epidemic diarrhoea virus, and analysis was performed using ELISA and Western blotting. Three cell strains specifically recognised the porcine epidemic diarrhoea virus nucleocapsid protein (N protein). In this study, we report the characterisation of effective tools for the establishment of porcine epidemic diarrhoea virus diagnostic methods and we have specifically generated primary antibodies for ELISA, IFA, test strip and Western blotting. Keywords: porcine epidemic diarrhoea virus, PEDV, nucleocapsid protein, N protein, monoclonal antibodies, MAbs Journal: Veterinární medicína Pages: 468-475 Volume: 63 Issue: 10 Year: 2018 DOI: 10.17221/23/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/23/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201810-0002.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:10:id:23-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H.N. Araujo Author-Name: G.B. Oliveira Author-Workplace-Name: Faculty of Veterinary Medicine, Federal University of the Semi-Arid Region, Mossoro, Brazil Author-Name: H.S. Costa Author-Workplace-Name: Faculty of Veterinary Medicine, Federal University of the Semi-Arid Region, Mossoro, Brazil Author-Name: P.M.A. Lopes Author-Workplace-Name: Faculty of Veterinary Medicine, Federal University of the Semi-Arid Region, Mossoro, Brazil Author-Name: R.E.M. Oliveira Author-Workplace-Name: Faculty of Veterinary Medicine, Federal University of the Semi-Arid Region, Mossoro, Brazil Author-Name: F.V.F. Bezerra Author-Workplace-Name: Faculty of Veterinary Medicine, Federal University of the Semi-Arid Region, Mossoro, Brazil Author-Name: C.E.B. Moura Author-Workplace-Name: Faculty of Veterinary Medicine, Federal University of the Semi-Arid Region, Mossoro, Brazil Author-Name: M.F. Oliveira Author-Workplace-Name: Faculty of Veterinary Medicine, Federal University of the Semi-Arid Region, Mossoro, Brazil Title: Anatomy of the brachial plexus in the Mongolian gerbil (Meriones unguiculatus Milne-Edwards, 1867) Abstract: The Mongolian squirrel is a murine rodent widely used as an experimental model and kept as a pet. With respect to the significance of the brachial plexus, we aimed to describe the origin and the nerves that constitute it, providing new information about the neuroanatomy of this species. Twenty adult animals were used, which were fixed in a 10% formaldehyde solution at the Applied Animal Morphophysiology Laboratory of the Brazilian Federal University of the Semi-Arid Region (UFERSA). The animals were medially incised with an opening of the thoracic cavity, and then bilateral dissection was carried out. Once the nerves were identified, cotton wool soaked with a 2% hydrogen peroxide solution was added for bleaching and better visualisation of structures. The gerbil brachial plexus derived from ventral roots of the fourth, fifth, sixth, seventh and eighth cervical nerves (C4-C8) and the first thoracic root (T1). The suprascapular nerve was formed from the nerve roots C4 and C5; the subscapular and cranial pectoral nerves from C5 and C6; the musculocutaneous and axillary nerves from C5, C6 and C7; and the caudal pectoral, radial, median, ulnar, thoracodorsal, lateral thoracic and long thoracic nerves from C7, C8 and T1. This resembles what is described in other rodents, such as the rat and capybara. The gerbil has a pre-fixed plexus conformation. Keywords: neuroanatomy, innervation, thoracic limb, expimental model, murine Journal: Veterinární medicína Pages: 476-481 Volume: 63 Issue: 10 Year: 2018 DOI: 10.17221/78/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/78/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201810-0003.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:10:id:78-2017-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.W. Lee Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: A.K. Elfadl Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea Author-Name: M.J. Chung Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea Author-Name: H.M. Arif Ullah Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea Author-Name: D.K. Yuh Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: S.H. Lee Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: K.S. Jeong Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea Author-Name: J.K. Park Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Title: Urocystitis, pyelonephritis, renal papillary necrosis and chronic tubulointerstitial disease causing chronic renal insufficiency in a Siberian tiger (Panthera tigris altaica): a case report Abstract: The present case report describes a case of chronic renal failure characterised by renal medullary fibrosis and renal papillary necrosis in a male Siberian tiger (Panthera tigris altaica). A 12-year-old male Siberian tiger presented with depression, anorexia and weight loss. Blood urea nitrogen (> 50.4 mmol/l) and ammonia (71.7 µmol/l) were increased, suggesting chronic renal failure and uraemia. The tiger died secondary to gastric haemorrhage. At necropsy, the kidneys had yellow lesions in the medulla and renal papillae and petechiae in the cortex. The stomach had multiple mucosal ulcers and haemorrhage. Microscopically, marked renal medullary fibrosis and renal papillary necrosis were observed with tubular atrophy, degeneration, coagulative necrosis, calcification and chronic inflammatory cell infiltration. The renal cortex showed moderate interstitial inflammation. The urinary bladder exhibited epithelial desquamation and submucosal fibrosis. The tiger was diagnosed with chronic renal failure secondary to renal papillary necrosis and medullary fibrosis. Keywords: chronic renal failure, emesis, gastric ulcer, papillary necrosis, renal fibrosis, uraemia, feline Journal: Veterinární medicína Pages: 482-487 Volume: 63 Issue: 10 Year: 2018 DOI: 10.17221/132/2017-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/132/2017-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201810-0004.txt Handle: RePEc:caa:jnlvet:v:63:y:2018:i:10:id:132-2017-VETMED