Template-Type: ReDIF-Article 1.0 Author-Name: Maja Mauric Author-Workplace-Name: Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Tomislav Masek Author-Workplace-Name: Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Darija Bendelja Ljoljic Author-Workplace-Name: Department of Dairy Science, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia Author-Name: Jozo Grbavac Author-Workplace-Name: Faculty of Agricultural and Foodbiotechnology, University of Mostar, Mostar, Bosnia and Hercegovina Author-Name: Kristina Starcevic Author-Workplace-Name: Department of Forensic and Judicial Veterinary Medicine, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Effects of different variants of the FASN gene on production performance and milk fatty acid composition in Holstein × Simmental dairy cows Abstract: Crossbreeding of Holstein cattle with bulls of other breeds has garnered increasing interest with respect to efforts to improve performance traits and to decrease problems with fertility and health. Therefore, the objective of this study was to compare the effects of the fatty acid synthase (FASN) gene on milk production traits and fatty acid composition of Simmental and first-generation Holstein × Simmental crossbred cows. A total of 105 cows (72 Simmental and 33 crossbred Holstein) were genotyped using the PCR-RFLP method and their fatty acid profiles were analysed. The crossbred Holstein cows with diplotype TW/AR had significantly higher fat content and yield compared to the purebred Simmental cows of the same diplotype. The Holstein × Simmental cows with the diplotype AR/AR were also characterised by significantly lower content of C16:0 and saturated fatty acids, but higher C18:1n9, monounsaturated fatty acid and monounsaturated fatty acid/saturated fatty acid content compared to the same diplotype of the Simmental cattle. These results indicate that with accurate breeding plans, crossbreeding Holstein cows with Simmental bulls could be directed towards a more desirable fatty acid composition of milk and dairy products. Keywords: milk production, fatty acid synthase, crossbreeding Journal: Veterinární medicína Pages: 101-108 Volume: 64 Issue: 3 Year: 2019 DOI: 10.17221/73/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/73/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201903-0001.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:3:id:73-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Sven Mencik Author-Workplace-Name: Department of Animal Breeding and Livestock Production, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Vlado Vukovic Author-Workplace-Name: Department of Livestock Production, Faculty of Agricultural Sciences and Food, Skopje, Macedonia Author-Name: Marija Spehar Author-Workplace-Name: Croatian Agricultural Agency, Zagreb, Croatia Author-Name: Mario Modric Author-Workplace-Name: Krmiva Ltd., Zagreb, Croatia Author-Name: Mario Ostovic Author-Workplace-Name: Department of Animal Hygiene, Behaviour and Welfare, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Anamaria Ekert Kabalin Author-Workplace-Name: Department of Animal Breeding and Livestock Production, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Association between ESR1 and RBP4 genes and litter size traits in a hyperprolific line of Landrace × Large White cross sows Abstract: This study was aimed at analysing single-nucleotide polymorphisms in the oestrogen receptor 1 (ESR1) and retinol-binding protein 4 (RBP4) genes in a hyperprolific line of Landrace × Large White (Topigs 20) cross sows (n = 101). The following litter size traits were analysed: total number born, number of born alive and number of weaned piglets. ESR1 and RBP4 genotypes determined on the basis of single-nucleotide polymorphisms were analysed using the least square method with the GLM procedure in SAS with eight effects. The REG procedure was used to calculate the effects of the additive and dominance components. The second parity sows with ESR1 BB genotype had a significantly higher (P < 0.05) number of weaned piglets compared to AB, with a tendency towards difference (P < 0.1) between homozygotes for number of born alive and number of weaned piglets. In the case of the RBP4 gene, the first parity sows of the AA genotype had a significantly higher total number born (P < 0.05) compared with the BB genotype, with a tendency towards difference (P < 0.1) between AA and heterozygotes for total number born, and homozygotes for number of born alive. The BB genotype showed a tendency for higher number of weaned piglets (P < 0.1) as compared with the AA genotype in the third parity sows for the RBP4 gene. In all parities, significant effects (P < 0.05) of parity were recorded for total number born, number of born alive and number of weaned piglets, season of farrowing for total number born, and the ESR1 and RBP4 interaction for number of born alive. In the first parity sows, significant effects (P < 0.05) on total number born were determined for gene interaction and gestation length, the latter also being recorded in the second parity sows. The additive (a) effect of single-nucleotide polymorphisms in RBP4 was significant (P < 0.05) for total number born in all parities as well as in the first parity sows, and dominance effect (d) (P < 0.05) of single-nucleotide polymorphisms in ESR1 for number of weaned piglets in the third parity sows. The obtained results regarding the investigated genes could help to provide a better understanding of the effect of single-nucleotide polymorphisms on litter size and thus promote genetic progress in pig reproduction management. Keywords: candidate gene, pigs, reproductive trait, SNPs Journal: Veterinární medicína Pages: 109-117 Volume: 64 Issue: 3 Year: 2019 DOI: 10.17221/87/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/87/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201903-0002.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:3:id:87-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Muhammed Enes Inanc Author-Workplace-Name: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey Author-Name: Sukru Gungor Author-Workplace-Name: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey Author-Name: Caner Ozturk Author-Workplace-Name: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Turkey Author-Name: Firat Korkmaz Author-Workplace-Name: International Center for Livestock Research and Training, Ankara, Turkey Author-Name: Ilktan Bastan Author-Workplace-Name: International Center for Livestock Research and Training, Ankara, Turkey Author-Name: Beste Cil Author-Workplace-Name: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Title: Cholesterol-loaded cyclodextrin plus trehalose improves quality of frozen-thawed ram sperm Abstract: The objective of this study was to determine effects of supplementing Tris-based semen extenders with either cholesterol-loaded cyclodextrin (CLC) or 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) plus trehalose (T) for cryopreservation of ram semen. Semen was collected with an artificial vagina from five Merino rams (2-3 years of age) during the non-breeding season. Ejaculates were pooled, divided into eight equal portions, diluted with a standard Tris-based extender containing: no additive (control); T (50 mM); or T (50 mM) + 1.5, 2.5 or 3.5 mg of either 7-DCLC or CLC. Semen was chilled from 37°C to 4°C, placed in 0.25 ml French straws, held 5 cm above liquid nitrogen for 12 minutes, then plunged into liquid nitrogen. After thawing, a computer-aided semen analyzer system (CASA) was used to assess motility, whereas plasma membrane and acrosome integrity (PMAI) and high mitochondrial membrane potential (HMMP) were assessed with flow cytometry. Sperm supplemented with 2.5 mg and 3.5 mg CLC + T had the highest (P < 0.05) total and progressive motility (65.2 ± 4.7 and 19.0 ± 1.0% respectively, mean ± SEM), albeit with no significant differences from sperm with 1.5 or 3.5 mg CLC + T. Sperm with 2.5 mg CLC + T had the highest (P < 0.05) PMAI (59.3%; not different from 3.5 mg CLC + T) and highest (P < 0.05) HMMP (64.6%; not different from 1.5 or 3.5 mg CLC + T). The lowest ALH value, 2.8 ± 0.3 µm was in the 2.5 mg 7-DCLC + T group; otherwise, there were no significant differences among groups for any other CASA end point. In conclusion, adding CLC + T to a tris-based extender optimized quality of frozen-thawed ram semen. Therefore, extenders including CLC + T have potential to improve quality of frozen-thawed ram sperm. Keywords: 7-dehydrocholesterol, semen cryopreservation, computer-assisted semen analyzer, flow cytometry Journal: Veterinární medicína Pages: 118-124 Volume: 64 Issue: 3 Year: 2019 DOI: 10.17221/146/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/146/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201903-0003.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:3:id:146-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Jaroslava Lidova Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: Milos Buric Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: Antonin Kouba Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: Josef Velisek Title: Acute toxicity of two pyrethroid insecticides for five non-indigenous crayfish species in Europe Abstract: Pyrethroid insecticides are highly toxic to many aquatic organisms. The aim of this study was to evaluate the toxicity of the commercial products Cyperkill 25 EC (active compound 250 g/l cypermethrin) and Decis Mega (active compound 50 g/l deltamethrin) for European non-indigenous marbled crayfish Procambarus virginalis, red swamp crayfish Procambarus clarkii, signal crayfish Pacifastacus leniusculus, spiny-cheek crayfish Orconectes limosus and yabby Cherax destructor. These data will provide a baseline for potential programmes to eradicate alien crayfish from Europe (EU Regulation No. 1143/2014; Commission Implementing Regulation No. 2016/1141) and are also relevant globally. The 96hLC50 values of Cyperkill 25 EC were 0.09, 0.17, 0.18, 0.19 and 0.30 µg/l for spiny-cheek crayfish, red swamp crayfish, marbled crayfish, signal crayfish and yabby, respectively. In the same order, the 96hLC50 values of Decis Mega were 0.76, 0.16, 0.21, 0.03 and 0.27 µg/l. The toxicity of the insecticides was similar and species-specific, possibly reflecting the size difference of the tested animals. This study shows that cypermethrin and deltamethrin are highly toxic to the tested crayfish species at low concentrations. This high sensitivity, along with the low accumulation in the food chain and short-term persistence in the aquatic environment, suggests that they are suitable biocides for eradicating crayfish in the wild. Stagnant, closed water bodies with newly emerging invaders are ideal sites for possible application, although local conditions must be considered. Keywords: biological invasion, insecticide, cypermethrin, deltamethrin, eradication, invasive species Journal: Veterinární medicína Pages: 125-133 Volume: 64 Issue: 3 Year: 2019 DOI: 10.17221/136/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/136/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201903-0004.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:3:id:136-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Chul Ho Park Author-Workplace-Name: Veterinary Teaching Hospital, Chonnam National University, Gwangju, Republic of Korea Author-Name: Chang Ho Son Author-Workplace-Name: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Title: Segmental agenesis of the uterine body, cervix and vagina in a bitch Abstract: This report describes a case of congenital segmental agenesis of the uterine body, cervix and vagina in a bitch. A five-year-old, Pomeranian bitch was presented with anorexia, abdominal distension and absence of the oestrus vulvar discharge. Complementary examinations were performed and ultrasonography revealed marked distention of the uterine horns with fluid-filled thin wall and defects in the uterine-vaginal segment. During surgery, closer inspection of the reproductive tract showed segmental agenesis of the uterine body, cervix and cranial part of vagina. The dog recovered uneventfully and six months later there were no further specific problems. Keywords: Müllerian duct, congenital defect, uterine-vaginal segment Journal: Veterinární medicína Pages: 134-137 Volume: 64 Issue: 3 Year: 2019 DOI: 10.17221/139/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/139/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201903-0005.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:3:id:139-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Carlos F. Agudelo Author-Workplace-Name: Small Animal Clinic, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: Michal Crha Author-Workplace-Name: Small Animal Clinic, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: Zeki Yilmaz Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey Author-Name: Branislav Lukac Author-Workplace-Name: Small Animal Clinic, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovakia Title: Gerbode defect in a dog Abstract: An intracardiac communication between the left ventricle and the right atrium (Geborde defect) was diagnosed in a 9-year Yorkshire Terrier with a history of chronic exercise intolerance. The history, clinical examination, and diagnostic imaging confirmed the diagnosis and did not reveal evidence of trauma or endocarditis which could lead to this special type of left-to-right shunting. A Gerbode defect is a very rare finding in human beings and animals. In the veterinary literature all reports about this condition were related to thoracic trauma or valvular infection. According to the authors, this would be the first clinical case of congenital Geborde defect in a dog. Keywords: canine, cushion defect, echocardiography, ventricular septal defect Journal: Veterinární medicína Pages: 138-143 Volume: 64 Issue: 3 Year: 2019 DOI: 10.17221/102/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/102/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201903-0006.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:3:id:102-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Chul Ho Park Author-Workplace-Name: Veterinary Teaching Hospital, Chonnam National University, Gwangju, Republic of Korea Author-Name: Jae Bok Han Author-Workplace-Name: Department of Radiological Science, Dongshin University, Jeonlanamdo, Republic of Korea Author-Name: Sang Ik Park Author-Workplace-Name: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Title: Dual infection with Entamoeba invadens and Aeromonas hydrophila in a captive anaconda (Eunectes murinus) leading to necrotising gastroenteritis and hepatocyte death Abstract: A ten-year-old male captive anaconda (Eunectes murinus) with a history of anorexia was found dead in Gwangju Uchi Zoo. Pathologically, this case showed multiple necrotising gastroenteritis ulcers, the death of large numbers of hepatocytes and multiple instances of chronic active interstitial nephritis. Entamoeba invadens and Aeromonas hydrophila were frequently found in these lesions. Confirmation of these pathogens was made using molecular and phylogenetic analyses. The TUNEL assay revealed the apoptotic nature of hepatocyte cell death. Keywords: snake, E. invadens, A. hydrophila, coinfection Journal: Veterinární medicína Pages: 144-148 Volume: 64 Issue: 3 Year: 2019 DOI: 10.17221/140/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/140/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201903-0007.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:3:id:140-2018-VETMED