Template-Type: ReDIF-Article 1.0 Author-Name: Katarzyna Tajchman Title: Selected haematological indices in farmed male fallow deer (Dama dama) depending on the different conditions during the wintering period Abstract: Fallow deer (Dama dama) are the most common breeding species among farmed cervids in Poland. Monitoring the animals' health, nutritional status, and welfare are highly important aspects in their breeding. Haematological variables are important indicators for comparing the physiological status of the animals and for monitoring the changes in the organism related to the adaptation to the breeding conditions. The aim of this study was to assess the impact of the day's length and the total protein content in the diet for the farmed fallow deer on the selected haematological variables. The study demonstrated a significant decline in the mean corpuscular volume (MCV) and an increase of the mean corpuscular haemoglobin concentration (MCHC) (P < 0.05) in all the animals after the winter period. However, the animal group exposed to prolonged daylight exhibited a significant increase in the platelet distribution width (PDW) and platelet large cell ratio (P-LCR) (P < 0.05). In turn, the group receiving a lower amount of protein in the diet was characterised by a significant reduction in the platelet count (PLT) (P < 0.05). Thus, the length of daylight and the protein content of the diet for fallow deer exert a significant impact on several haematological characteristics, which may serve as indicators of an animal's nutritional status and welfare. Keywords: Dama dama, haematology, daylight length, total protein Journal: Veterinární medicína Pages: 379-385 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/3/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/3/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201909-0001.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:9:id:3-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Jana Jurankova Author-Workplace-Name: Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: Dagmar Jirsova Author-Workplace-Name: Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Ceske Budejovice, Czech Republic Author-Workplace-Name: Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic Author-Name: Barbora Pafco Author-Workplace-Name: Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: Pavel Forejtek Author-Workplace-Name: Department of Animal Protection, Welfare and Behaviour, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer (Capreolus capreolus L.) Abstract: The poor state of health and increased mortality rate of young roe deer, as reported by South Moravian hunters, caused by the increasing numbers of adult nematodes in the lungs of roe deer prompted us to identify the parasites using a combination of morphological measurements and a phylogenetic SSU rRNA analysis. The study was conducted in a 294 ha game reserve in South Moravia, Czech Republic. Molecular and morphometric techniques were used to identify adult nematodes collected from the respiratory tracts of nine 4-5 months old roe deer in poor health (low body weight of 3-4 kg, poor haircoat quality, and, in some cases, symptoms of diarrhoea). The morphological identification was based on a combination of adult worm characteristics corresponding to Dictyocaulus capreolus. A small subunit rRNA (SSU) partial sequence analysis showed the highest identity scores (99%) corresponding to the sequences of D. capreolus from a roe deer (GenBank: AY168859) from Sweden and the outcomes of the phylogenetic analyses resulted in a tree with a high branch support for two groups, with our sequences forming a well-supported clade with D. capreolus and Dictyocaulus sp. ex Capreolus capreolus (FJ589016) and Dictyocaulus sp. ex Rupicapra rupicapra (FJ589019) sequences from Spain. The examined roe deer have shown symptoms of diarrhoea, anorexia, and respiratory tract inflammation indicating that there might be a connection to the clinical importance of the Dictyocaulus infection. Keywords: lung nematodes, rRNA, respiratory tract, anorexia, diarrhoea Journal: Veterinární medicína Pages: 386-391 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/9/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/9/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201909-0002.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:9:id:9-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Katarina Matiaskova Author-Workplace-Name: Department of Immunology, Veterinary Research Institute, Brno, Czech Republic Author-Workplace-Name: Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: Katerina Nedbalcova Author-Workplace-Name: Department of Immunology, Veterinary Research Institute, Brno, Czech Republic Author-Name: Radek Tesarik Author-Workplace-Name: Department of Immunology, Veterinary Research Institute, Brno, Czech Republic Author-Name: Hana Kudlackova Author-Workplace-Name: Department of Immunology, Veterinary Research Institute, Brno, Czech Republic Author-Name: Jan Gebauer Author-Workplace-Name: Department of Immunology, Veterinary Research Institute, Brno, Czech Republic Author-Name: Miroslav Toman Author-Workplace-Name: Department of Immunology, Veterinary Research Institute, Brno, Czech Republic Author-Name: Martin Faldyna Author-Workplace-Name: Department of Immunology, Veterinary Research Institute, Brno, Czech Republic Title: A crude capsular polysaccharide extract as a potential novel subunit vaccine with cross-protection against the most prevalent serovars of Glaesserella (Haemophilus) parasuis in the Czech Republic Abstract: Glaesserella (Haemophilus) parasuis is a part of the normal flora of the respiratory tract of pigs. However, under certain conditions it can also induce severe systemic disease with high morbidity and mortality leading to gross economic losses in the pig industry. The most prevalent serovars in pig herds in the Czech Republic are the virulent serovars 1, 4, 5 and 13. The currently available commercial vaccines are inactivated vaccines with certain limitations, such as no or poor cross-serovar protection. Therefore, the aim of the present study was to construct a subunit vaccine with a crude capsular polysaccharide extract (cCPS) isolated from G. parasuis CAPM 6475 (serovar 5) and evaluate its immunogenicity in a mouse model. Mice were immunised subcutaneously with two doses of the constructed vaccine in a 14-day interval and challenged intraperitoneally with various G. parasuis strains (serovars 1, 4, 5, 13) at 21 days after the second immunisation. The results of the ELISA test showed that the boost dose of the vaccine induced the production of IgG antibodies in high levels. On the basis of the death cases, the pathological findings and the bacterial isolation, the mice immunised with the cCPS were partially protected against the challenge with the homologous serovar 5 as well as with heterologous serovars 1, 4 and 13 of G. parasuis. The cross-reaction of the mixed serum from the immunised mice with the tested serovars was seen in the western-blotting also. Moreover, the most abundant protein found in the cCPS by mass spectrometry was catalase, a protein of molecular weight 55 kDa that may correspond to the strongest reaction seen in the western-blotting. Our findings indicated that the crude capsular polysaccharide extract may provide an effective immunogenicity in preventing a G. parasuis infection caused by the most prevalent serovars in the Czech Republic. However, the evaluation of the efficacy needs to be performed in pigs before any conclusions can be drawn. Keywords: catalase, cross-serotype protection, mouse model, respiratory pathogen Journal: Veterinární medicína Pages: 392-399 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/71/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/71/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201909-0003.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:9:id:71-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Michela Pugliese Author-Workplace-Name: Department of Veterinary Science, University of Messina, Polo Universitario Annunziata, Messina, Italy Author-Name: Monica Ragusa Author-Workplace-Name: Department of Experimental and Clinical Medicine, University of Catanzaro, Viale Europa, Catanzaro, Italy Author-Name: Vito Biondi Author-Workplace-Name: Department of Veterinary Science, University of Messina, Polo Universitario Annunziata, Messina, Italy Author-Name: Annamaria Passantino Author-Workplace-Name: Department of Veterinary Science, University of Messina, Polo Universitario Annunziata, Messina, Italy Author-Name: Kai Zhang Author-Workplace-Name: Department of Traditional Chinese Veterinary Medicine. Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Xiaoxihu, Qilihe District, Lanzhou city, Gansu province, People's Republic of China Author-Name: Francesco Macri Author-Workplace-Name: Department of Veterinary Science, University of Messina, Polo Universitario Annunziata, Messina, Italy Title: The effect of moderate treadmill exercise on the resistive index of the medial long posterior ciliary artery in dogs Abstract: The resistive index (RI) is an indirect measurement of arterial resistance by means of a ratio between the peak systolic and end diastolic velocities recorded with a spectral Doppler device, especially used to evaluate the vascular damage in ocular diseases such as glaucoma. Some ocular variables such as the intraocular pressure (IOP), the choroidal thickness, the axial length and the ocular blood flow may be influenced by physical exercise. The purpose of this study was to evaluate the influence of the exercise on the RI of the medial long posterior ciliary artery in dogs, and correlate the data obtained with the IOP values. Ten clinically healthy dogs were subjected to moderate physical exercise on a canine motorised treadmill at different speeds for 45 minutes. A colour Doppler examination was performed and the RI values were calculated for the medial long posterior ciliary artery at rest, immediately after the exercise, and after 60 minutes at the end of the exercise. At the same times, the IOP was recorded by applanation tonometry. The data were analysed by a two-way repeated ANOVA measurement in order to compare the RI and the IOP. Wilcoxon's test was applied for the post hoc comparison. Spearman's rank correlation for non-normal distribution was used to determine a relationship between the RI and the IOP. The at rest RI was 0.722 +/-0.022, IOP 12.38 +/3.21 mm Hg. A significant decrease in the RI was observed immediately after the exercise (0.697 +/-0.035) and during the passive recovery phase (0.682 +/-0.042). A significant decrease in the IOP (11+/3.39 mmHg) was recorded after 60 min of the passive recovery phase; at the end of the exercise, a slight decrease (12.29+/4.26 mm Hg) mm Hg was detected. During the test, a linear correlation between the RI and the IOP was observed. Our results suggest that exercise induces the modification of the ophthalmic blood flow in dogs, presumably related to the compensatory neuro-hormonal mechanisms. Keywords: physical exercise, ocular variables, ocular blood flow Journal: Veterinární medicína Pages: 400-406 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/162/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/162/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201909-0004.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:9:id:162-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Hakhyun Kim Author-Workplace-Name: Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Author-Name: Ji-Houn Kang Author-Name: Byeong-Teck Kang Author-Workplace-Name: Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Author-Name: Mhan-Pyo Yang Author-Workplace-Name: Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Title: Hypoglycaemic crisis induced by non-islet cell tumours in two dogs Abstract: Two dogs were admitted for the diagnosis and treatment of neoplasia (a hepatic and a mammary tumour, respectively), lethargy, quadriparesis and abnormal mentation with hypoglycaemia. The blood analyses showed severe hypoglycaemia (1.32 and 1.60 mmol/l, respectively). Although prompt treatment, including intravenous administration of dextrose, was initiated, the blood glucose concentrations were not restored to a normal range. After the diagnostic procedures, no aetiology other than the hepatic tumour identified by the abdominal radiography and ultrasonography, and a mammary tumour that might have caused the hypoglycaemia, were identified. Because there was a high suspicion of non-islet cell tumour-induced hypoglycaemia as a paraneoplastic syndrome, the dogs underwent a hepatic lobectomy and total mastectomy with an ovariohysterectomy, respectively. Within 12 hours after surgery, the blood glucose concentrations of both cases had normalised, even without the administration of dextrose. The histopathological examinations identified a hepatocellular adenoma and a mammary carcinoma, respectively. The endocrine analysis of the serum at admission revealed low serum insulin concentrations (< 1.44 pmol/l) and high serum concentrations of insulin-like growth factor 2 in both dogs. Therefore, the diagnosis in both dogs was confirmed to be non-islet cell tumour-induced hypoglycaemia. Both dogs remained alive without the recurrence of hypoglycaemia 24 months later. Previously, the administration of intravenous dextrose has been considered as the initial treatment in dogs with hypoglycaemia; however, this can temporarily ameliorate the clinical signs related to the non-islet cell tumour-induced hypoglycaemia and help the anaesthesia for the surgical tumour resection as an emergency. Therefore, the definitive treatment of non-islet cell tumour-induced hypoglycaemia might be rapid surgical intervention, which can be associated with good prognosis in dogs with severe non-islet cell tumour-induced hypoglycaemia. Keywords: canine, glucagon, hypoglycaemia, insulin-like growth factor-2, paraneoplastic syndrome Journal: Veterinární medicína Pages: 407-416 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/145/2018-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/145/2018-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201909-0005.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:9:id:145-2018-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Jin-Ok Ahn Author-Workplace-Name: Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon, Republic of Korea Author-Name: Sang-Chul Park Author-Workplace-Name: Department of Veterinary Internal Medicine and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: Woo-Jin Song Author-Workplace-Name: Department of Veterinary Internal Medicine and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: Min-Ok Ryu Author-Workplace-Name: Department of Veterinary Internal Medicine and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: Qiang Li Author-Workplace-Name: Department of Veterinary Internal Medicine and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: Hyun-Jung Oh Author-Workplace-Name: Department of Veterinary Medical Imaging and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: Jin-Young Chung Author-Workplace-Name: Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon, Republic of Korea Author-Name: Min-Cheol Choi Author-Workplace-Name: Department of Veterinary Medical Imaging and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: Hwa-Young Youn Author-Workplace-Name: Department of Veterinary Internal Medicine and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Title: Aortic thromboembolism. A different predisposing disease in four dogs: a case report Abstract: Four dogs had signs of pelvic limb lameness, pain, and collapse resulting from an occlusion of the distal aorta or the iliac arteries by thrombi. The diagnosis of an aortic thromboembolism was based on the absence or weakness of a femoral pulse, and the two-dimensional and Doppler ultrasonography of the abdominal aorta, iliac, and femoral arteries. Three dogs had a concurrent disease predisposing to thrombosis, including hyperadrenocorticism, protein losing nephropathy, neoplasia, and cardiac disease. Three dogs were treated with a tissue plasminogen activator (t-PA) in an attempt to lyse the thrombosis; two regained pelvic limb function. The other two dogs died shortly after the diagnosis of a thrombosis. A complete description of the history, clinical signs, laboratory analysis and imaging studies is included. Moreover, a review of the aortic thromboembolism, a diagnosis protocol and the options for its treatment are discussed. Keywords: arterial thrombosis, tissue plasminogen activator, pelvic weakness, hind-limb paresis Journal: Veterinární medicína Pages: 417-421 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/83/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/83/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201909-0006.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:9:id:83-2019-VETMED