Template-Type: ReDIF-Article 1.0 Author-Name: Frantisek Zigo Author-Workplace-Name: Department of Animal Breeding Author-Name: Juraj Elecko Author-Workplace-Name: Department of Animal Breeding Author-Name: Milan Vasil Author-Workplace-Name: Department of Animal Breeding Author-Name: Silvia Ondrasovicova Author-Workplace-Name: Department of Anatomy, Histology and Physiology Author-Name: Zuzana Farkasova Author-Workplace-Name: Department of Animal Breeding Author-Name: Jana Malova Author-Workplace-Name: Department of Food Hygiene and Technology Author-Name: Ladislav Takac Author-Workplace-Name: Institute of Judicial and Public Veterinary Medicine and Economics Author-Name: Martina Zigova Author-Workplace-Name: Department of Pharmacology, Faculty of Medicine, University of Pavol Jozef Šafárik in Košice, Košice, Slovakia Author-Name: Jolanta Bujok Author-Workplace-Name: Department of Biostructure and Animal Physiology, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: Ewa Pecka-Kielb Author-Workplace-Name: Department of Biostructure and Animal Physiology, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: Petra Timkovicova-Lackova Author-Workplace-Name: Institute of Nutrition, Dietetics and Feed Production, University of Veterinary Medicine and Pharmacy, KoĹĄice, Slovakia Title: The occurrence of mastitis and its effect on the milk malondialdehyde concentrations and blood enzymatic antioxidants in dairy cows Abstract: Early identification of mastitis is a serious challenge for dairy farmers and veterinarians in ensuring the health of an animal and the hygienic quality of the produced milk. The purpose of this study was to detect the occurrence and aetiology of mastitis in a dairy herd of 153 milked cows localised in a farm in west Slovakia. During the complex investigation, 606 quarter milk samples were examined (6 quarters were discarded) and classified based on the clinical status, the presence of abnormal udder secretions, the result of the California mastitis test (CMT), the somatic cell count (SCC) and the bacteriological identification of the pathogens causing the intramammary infection (IMI). The study was augmented by the detection of malondialdehyde (MDA) in the milk and the measurements of the blood enzymatic activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) as potential biomarkers for the udder health screening. A positive CMT score was recorded in 19.5% (118) of the examined quarters and 12.5% (76) of the quarters were infected with bacterial pathogens causing latent mastitis (LM; 1.3%), subclinical mastitis (SM; 8.3%), and clinical mastitis (CM; 2.9%). The most commonly isolated bacteria from the infected quarters were coagulase-negative staphylococci (55.2%), Staphylococcus aureus (11.8%) and streptococci (10.5%). The concentration of MDA and SCC were significantly higher from both the SM and CM cases than in the milk samples from the healthy cows, while the blood activities of SOD and GPx were lower in the cows with CM compared to the healthy cows. The higher MDA concentrations in the SM and CM milk observed in this study showed the presence of an oxidative stress in the infected milk, accompanied by a decrease in the antioxidative enzymatic activity in the blood of the cows. Therefore, the measurement of the milk MDA concentration and the activity of the blood SOD and GPx may prove insightful for the better screening of the udder health in the early diagnosis of mastitis. Keywords: milking, biomarkers, lipid peroxidation, udder health, staphylococci Journal: Veterinární medicína Pages: 423-432 Volume: 64 Issue: 10 Year: 2019 DOI: 10.17221/67/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/67/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201910-0001.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:10:id:67-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Kamil Siatka Author-Workplace-Name: Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland Author-Name: Anna Sawa Author-Workplace-Name: Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland Author-Name: Mariusz Bogucki Author-Workplace-Name: Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland Author-Name: Dariusz Piwczynski Author-Workplace-Name: Department of Biotechnology and Animal Genetics, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland Author-Name: Sylwia Krezel-Czopek Author-Workplace-Name: Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland Title: The relationships between the somatic cell counts in the milk and the fertility of Polish Holstein-Friesian cows Abstract: The aim of the study was to analyse the relationship between the somatic cell counts in the milk (the udder health status indicator) and the cow's fertility, taking the influence of the selected factors into account. The udder health status was determined based on the somatic cell count of the milk from 88 745 test-day records from 55 685 Polish Holstein-Friesian cows. The test-day measurements were made up to 30 days before the first insemination, during the period up to 180 days of the first and second lactation. Because the somatic cell count showed high variation and is not normally distributed, the data were transformed to the natural logarithm scale. Based on the results of the statistical analysis, it was shown that with the increase in the number of somatic cells in the milk, the calving intervals were extended by 11 days (P ≤ 0.01), the service period by around 4 days (P ≤ 0.05), the increase in the services per conception by 0.11 (P ≤ 0.01). There was a statistically significant correlation, weak on the Guilford scale, between the natural logarithm of the somatic cell count and the fertility of the cows: the calving interval - CI (r = 0.050**, P ≤ 0.01), service period - SP (r = 0.016**) end services per conception - SPC (r = 0.019**). Monitoring the number of somatic cells in the milk could contribute to improving the fertility of the cows in particular: in the second lactation in the double lactation (for the CI (r = 0.059**)); in herds with a production level of 7000-9000 kg of milk (for the CI (r = 0.055**), the SP (r = 0.022**) and the SPC (r = 0.024**)); the daily productivity of > 40 kg (for the CI (r = 0.052**), the SP (r = 0.033**) and the SPC (r = 0.029**)), the number of cows in the herd of > 200 (for the CI (r = 0.061**), the SP (r = 0.034**) and the SPC (r = 0.033**)), in the autumn season of the first insemination (for the CI (r = 0.072**), the SP (r = 0.027**) and the SPC (r = 0.031**)). The magnitude of these correlations varied within the classes of the factors such as the daily production level, the age of cows´ lactation number, the season of the first insemination, the herd production level, and the herd size. It appears that the somatic cell count results obtained from the periodic milk recording, considered as an indirect measure of the udder health and used when deciding on the mastitis treatment, could be a useful tool for controlling the fertility in the cows. Keywords: cow fertility, mastitis, correlations, calving interval, service period, services per conception Journal: Veterinární medicína Pages: 433-439 Volume: 64 Issue: 10 Year: 2019 DOI: 10.17221/34/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/34/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201910-0002.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:10:id:34-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Ramazan Yildiz Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Mehmet Akif University, Burdur, Turkey Author-Name: Mahmut Ok Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey Author-Name: Merve Ider Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey Author-Name: Aybars Akar Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Mehmet Akif University, Burdur, Turkey Author-Name: Amir Naseri Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey Author-Name: Erman Koral Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey Title: The changes in biomarkers for necrotising enterocolitis in premature calves with respiratory distress syndrome Abstract: The aim of this study was to determine the changes of the biomarkers used for the diagnosis of necrotising enterocolitis of human neonates in premature calves with respiratory distress syndrome (RDS). Novel biomarkers including the intestinal fatty acid binding protein (IFABP), the liver-type FABP (LFABP), trefoil factor-3 (TFF3), actin gamma 2 smooth muscle (ACTG2), and Claudin-3 were investigated using bovine specific ELISA kits. Thirty premature calves with respiratory distress syndrome (the RDS group), seven premature calves without RDS (the non-RDS group), and seven healthy calves (control) were included in the study. Blood samples from all the groups were taken at 0 and 48 h for the blood gas and biomarker measurement. It was determined that IFABP (P < 0.05), LFABP (P < 0.05), TFF3 (P < 0.05), ACTG2 (P < 0.05), and Claudin-3 (P < 0.05) in the control group were significantly higher than those in the RDS and non-RDS groups at 0 hour. The LFABP and Claudin-3 concentrations in the control group were statistically higher (P < 0.05) than those in the RDS and non-RDS groups at 48 h, whereas the ACTG2 and TFF3 contents were significantly higher (P < 0.05) than the non-RDS group. A significant increase in the contents of IFABP (P ≤ 0.01), LFABP (P < 0.05), TFF3 (P < 0.05), ACTG2 (P < 0.05) at 48 h was detected in the RDS group only. In conclusion, the changes in the biomarkers support the suspicion of intestinal damage such as necrotising enterocolitis (NEC) after enteral feeding in premature calves with RDS. Intestinal damage biomarkers such as IFABP, LFABP, TFF3, and ACTG2 may be useful in the diagnosis of intestinal damage in premature calves. These results also indicate that the plasma concentrations of the intestinal biomarkers change in new born calves with their gestational age. Keywords: actin, claudin, FABP, hypoxia, trefoil factor Journal: Veterinární medicína Pages: 440-447 Volume: 64 Issue: 10 Year: 2019 DOI: 10.17221/37/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/37/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201910-0003.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:10:id:37-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Petr Rauser Author-Name: Hana Nemeckova Author-Workplace-Name: Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: Marketa Mrazova Author-Workplace-Name: Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: Jana Burova Author-Workplace-Name: Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: Lukas Novak Author-Workplace-Name: Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: The influence of fentanyl injection followed by infusion on the intraocular pressure, pupil size and aqueous tear production in healthy non-painful dogs Abstract: The goal of the presented research was to assess the influence of continuously administered fentanyl on the intraocular pressure, pupil size and aqueous tear production in dogs. A prospective, randomised, double "blind" clinical study was performed. Twenty-five non-painful dogs, 13 breeds, a body weight of 10.0 ± 5.4 kg (mean ± SD) and age of 6.5 ± 3.3 years, 12 males and 13 females with no ocular abnormalities were randomly allocated into two groups receiving an intravenous injection of saline (SAL) 0.3 ml/kg followed by an infusion 2 ml/kg/h or an intravenous injection of fentanyl (FEN) 0.005 mg/kg (diluted in 0.3 ml/kg) followed by an infusion 0.005 mg/kg/h (diluted in 2 ml/kg/h). The intraocular pressure (IOP), pupil size (PS), pulse rate (PR), respiratory frequency (fR) and systolic and diastolic arterial pressures (SAP, DAP) were measured before (baseline) and at 2, 5, 10, 20 and 30 minutes after the premedication. The Schirmer Tear Test I (STT-I) was measured prior to and at 30 min after the premedication. The data were analysed by Bartlett's, Anderson-Darling and Dunnett's tests, the t-test and an analysis of variance (ANOVA) (P < 0.05). Relative to the baseline, in the fentanyl group, the PS was significantly decreased at all time points, the PR was significantly decreased at T30 and the fR was significantly decreased at T5, T10, T20 and T30. There were no other significant changes in the IOP, STT-I, SAP and DAP relative to the baseline. Compared to the control group, in the fentanyl group, the PS was significantly smaller at T2, T5, T10, T20 and T30, the PR was significantly lower at T2, T20 and T30 and the fR was significantly higher at T20. Within thirty minutes of a constant rate infusion of fentanyl in the healthy non-painful dogs, the intraocular pressure and aqueous tear production were not affected. However, the fentanyl significantly decreased pupil size. This fact should be considered, when planning analgesia where miosis is undesirable. Keywords: canine, analgesia, opioids, pupil diameter, CRI Journal: Veterinární medicína Pages: 448-455 Volume: 64 Issue: 10 Year: 2019 DOI: 10.17221/94/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/94/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201910-0004.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:10:id:94-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Tae Geum Kim Author-Workplace-Name: Center for Jeongup Industry-Academy-Institute Cooperation, Chonbuk National University, Jeollabuk-do, Republic of Korea Author-Name: Bong Jo Kang Author-Workplace-Name: Ocean and Fisheries Research Institute, Jeju special self-governing province, Republic of Korea Author-Name: Sang Ik Park Author-Workplace-Name: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Author-Name: Tae Jung Kim Author-Workplace-Name: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Title: Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco Abstract: The viral haemorrhagic septicaemia virus (VHSV) causes high mortality in many marine and freshwater fish species, resulting in heavy economic losses in fish farming. Previously, cholera toxin B subunit (CTB)-fused recombinant viral haemorrhagic septicaemia virus glycoproteins (rec-VHSV-GPs) have been successfully expressed in tobacco, Nicotiana benthamiana. Here, we evaluated the potential of rec-VHSV-GPs as an oral vaccine against a live viral challenge. After immunisation of mice and fish (olive flounder, Paralichthys olivaceous) with those antigenic proteins in a feed additive form, the antibody titres were increased statistically, especially in the primed groups (P < 0.0001) in both the mouse and fish. After the viral challenge under low water temperature culture conditions (below 18 °C), the immunised fish were protected successfully against the challenge, showing a significantly lower mortality rate (P < 0.05). This result suggests that this plant-based immunisation system could induce an effective immune response. It could be used as a candidate to develop an oral vaccine for fish. Keywords: flounder, oral vaccine, plant expression, viral haemorrhagic septicaemia virus Journal: Veterinární medicína Pages: 456-461 Volume: 64 Issue: 10 Year: 2019 DOI: 10.17221/26/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/26/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201910-0005.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:10:id:26-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Minho Ko Author-Workplace-Name: Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon-si, Gangwon-do, Republic of Korea Author-Name: Tae Hyeong Kim Author-Workplace-Name: Department of Veterinary Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon-si, Gangwon-do, Republic of Korea Author-Name: Yoonhwan Kim Author-Workplace-Name: Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon-si, Gangwon-do, Republic of Korea Author-Name: Doo Kim Author-Workplace-Name: Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon-si, Gangwon-do, Republic of Korea Author-Name: Jin-Ok Ahn Author-Workplace-Name: Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon-si, Gangwon-do, Republic of Korea Author-Name: Byung-Jae Kang Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Kangwon National University, Chuncheon-si, Gangwon-do, Republic of Korea Author-Name: Sooyoung Choi Author-Workplace-Name: Department of Veterinary Diagnostic Imaging, College of Veterinary Medicine, Kangwon National University, Gangwondo, Republic of Korea Author-Name: Inchul Park Author-Workplace-Name: Department of Veterinary Diagnostic Imaging, College of Veterinary Medicine, Kangwon National University, Gangwondo, Republic of Korea Author-Name: Jung Hoon Choi Author-Workplace-Name: Department of Veterinary Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon-si, Gangwon-do, Republic of Korea Author-Name: Jin Young Chung Author-Workplace-Name: Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon-si, Gangwon-do, Republic of Korea Title: Improvement of systemic lupus erythematosus in dogs with canine adipose-derived stem cells Abstract: A 6-year-old, intact female, Maltese presented with limited movement of the hind limbs and intermittent pruritus for three months. The patient was diagnosed with systemic lupus erythematosus. Conventional immunosuppressive therapy was attempted for 70 days; however, the patient still suffered from life-threatening pancreatitis and hepatopathy. Therefore, we tried canine adipose-derived mesenchymal stem cells for immunomodulation and liver protection. After 6-months of the stem cell therapy, the patient's walking and hepatopathy improved. These findings indicate that stem cell therapy may be another option for systemic lupus erythematosus in dogs. Keywords: dog, SLE, stem cell therapy Journal: Veterinární medicína Pages: 462-466 Volume: 64 Issue: 10 Year: 2019 DOI: 10.17221/46/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/46/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201910-0006.txt Handle: RePEc:caa:jnlvet:v:64:y:2019:i:10:id:46-2019-VETMED