Template-Type: ReDIF-Article 1.0 Author-Name: H Sukur Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Near East University, Nicosia, North Cyprus Author-Name: OM Esendal Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Near East University, Nicosia, North Cyprus Title: Presence and antimicrobial resistance of coagulase-negative staphylococci isolated from animals in a Veterinary Teaching Hospital in Cyprus Abstract: Coagulase-negative staphylococci (CoNS) are a group of commensal microorganisms residing on the skin and mucous membranes of both humans and animals. Until recently, they have been regarded as non-pathogenic to livestock and companion animals, but since then, their clinical importance in veterinary medicine has increased with the discovery of their potential pathogenic roles in animals causing skin and soft tissue infections together with spontaneous abortions and mastitis. Scientific data concerning the presence of CoNS in North Cyprus are very limited. Therefore, the purpose of the study reported herein was to investigate the presence and antimicrobial resistance patterns of CoNS species isolated from various animals presented at the Veterinary Teaching Hospital in North Cyprus between July 2018 and 2019. Staphylococci were isolated from 37.0% (87/235) of the samples submitted, within which 60.9% (53/87) and 39.1% (34/87) were identified as coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS), respectively. Among the CoNS, S. chromogenes was the most predominantly isolated species (14/34, 41.2%), followed by S. capitis (5/34, 14.7%) and S. simulans (4/34, 11.8%). Of these 34 CoNS, 24 (70.6%) and 10 (29.4%) were identified as MRCoNS and MSCoNS, respectively. The CoNS isolates showed relatively high levels of resistance towards amoxicillin/clavulanic acid (19/34, 55.9%), tetracycline (14/34, 41.2%) and penicillin (13/34, 38.2%). In conclusion, the presence of CoNS, especially MRCoNS, and the detection of multiple drug resistant (MDR) species with a high prevalence were regarded as being important since they might limit and have negative effects on the therapeutic treatment options of staphylococcal infections in animals, and might have both public and veterinary concerns. Keywords: cows, companion animals, CoNS, North Cyprus, prevalence Journal: Veterinární medicína Pages: 191-198 Volume: 65 Issue: 5 Year: 2020 DOI: 10.17221/166/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/166/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202005-0001.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:5:id:166-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: MSA Faruk Author-Workplace-Name: Department of Animal Science and Technology, Sunchon National University, Suncheon, Jeonnam, Republic of Korea Author-Name: B Park Author-Workplace-Name: Animal Clinic of Hongseong Naknong Nonghyup, Republic of Korea Author-Name: S Ha Author-Workplace-Name: Dairy Science Division, Department of Animal Resources Development, National Institute of Animal Science, Republic of Korea Author-Name: SS Lee Author-Workplace-Name: Department of Animal Science and Technology, Sunchon National University, Suncheon, Jeonnam, Republic of Korea Author-Name: LL Mamuad Author-Workplace-Name: Department of Animal Science and Technology, Sunchon National University, Suncheon, Jeonnam, Republic of Korea Author-Name: Y Cho Author-Workplace-Name: Department of Animal Science and Technology, Sunchon National University, Suncheon, Jeonnam, Republic of Korea Title: Comparative study on different field tests of ketosis using blood, milk, and urine in dairy cattle Abstract: This study assessed the diagnostic performance of five field tests for ketosis in the blood, milk and urine in dairy cows. Samples were collected simultaneously from 102 dairy cows to determine the accuracy of the test results. The concentration of β-hydroxybutyrate (BHBA) in the blood was measured quantitatively by using a portable ketone test kit. The milk and urine samples were analysed semi-quantitatively using different commercial test kits and urine sticks. The animals were categorised into a subclinical ketosis (SCK) and clinical ketosis (CK) group based on the BHBA concentration in the blood. The diagnostic performance of the milk and urine test kits was compared with the blood test kit. In the blood test, out of 102 cows, 27 and 19 cows were diagnosed with SCK and CK, respectively. The percentage agreement (kappa value) for the three different milk tests and the urine test kit was 43% (κ = -0.181), 72% (κ = 0.243), 63% (κ = 0.065) and 64% (κ = 0.163), respectively, for the SCK detection, which indicates lower sensitivity. However, a strong agreement with 88% (κ = 0.612), 85% (κ = 0.505), 86% (κ = 0.528) and 91% (κ = 0.753) was observed in the three milk tests and the urine test, respectively, for the CK diagnosis, showing a higher sensitivity. For the diagnosis of the SCK and CK, all the tested kits are generally applicable for the detection of CK in dairy cattle, but caution is needed during the interpretation of the SCK data due to the low sensitivity of the milk and urine tests for the SCK detection. Furthermore, this study suggests that milk and urine samples can be used as alternatives for the diagnosis of CK in replacement of blood tests. Keywords: β, -hydroxybutyrate, clinical ketosis, subclinical ketosis, diagnosis, semi-quantitative determination Journal: Veterinární medicína Pages: 199-206 Volume: 65 Issue: 5 Year: 2020 DOI: 10.17221/69/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/69/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202005-0002.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:5:id:69-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D Zapletal Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: L Kudelkova Author-Workplace-Name: Department of Animal Protection and Welfare and Public Veterinary Medicine, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: P Jakesova Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: V Simek Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: E Strakova Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: P Suchy Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Title: Dehulling effect of dietary administered white lupine seeds on the blood biochemistry of broilers Abstract: The aim of the study was to assess the effect of the partial or complete dietary replacement of crude protein (CP) from soybean meal (SBM) with CP from the meal of whole (trial 1) or dehulled (trial 2) white lupine (WL) seeds on the blood plasma biochemical indicators in broilers. Chickens of the control (C) group were fed diets containing the SBM as the main protein source in the feed. It results from the findings that the complete and also partial replacement of CP from the SBM by CP from the meal of whole WL seeds in diets led to a decrease in the plasma cholesterol and triacylglycerides (TAG) levels (P < 0.001). Concerning the dietary administration of dehulled WL seeds, only the complete replacement of CP resulted in a decrease in the plasma glucose and TAG levels in 35-day-old broilers (P < 0.05). In addition, when including the dehulled WL seeds into the diet, an increase in the plasma aspartate aminotransferase activity was found in both the E1 and E2 groups (P < 0.01). Based on the results found, it can be stated that the use of meal from whole WL seeds seems to be a promising protein feed component which has beneficial effects on the health of broilers. Keywords: feed, lupine kernel, meat-type chickens, plasma biochemical indicators, whole seeds, Zulika variety Journal: Veterinární medicína Pages: 207-214 Volume: 65 Issue: 5 Year: 2020 DOI: 10.17221/27/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/27/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202005-0003.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:5:id:27-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Marchegiani Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (MC), Italy Author-Name: A Spaterna Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (MC), Italy Author-Name: A Palumbo Piccionello Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (MC), Italy Author-Name: M Meligrana Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (MC), Italy Author-Name: A Fruganti Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (MC), Italy Author-Name: AM Tambella Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (MC), Italy Title: Fluorescence biomodulation in the management of acute traumatic wounds in two aged dogs Abstract: Traumatic wounds represent a fairly common reason for the admission of dogs to a veterinary practice. In elderly patients, the management of wound healing can be challenging due to both the concurrent diseases and impaired physiological states, potentially resulting in delayed healing and chronic or non-healing wounds. The aim of this article is to describe an innovative therapy based on photobiomodulation (PBM) for the management of acute traumatic wounds with significant tissue loss in aged dogs. Two mixed breed dogs were presented with similar wounds in the cervical region and were managed with a fluorescence biomodulation system, a form of PBM, which consists of a photoconverter topical gel that is illuminated with a blue light-emitting diode lamp, applied on a weekly basis. Wound closure was achieved after 9 and 16 weekly treatments, respectively, with a complete re-epithelisation of the skin. This fluorescence-generating system is an innovative, non-invasive, wound care therapy and these results indicate it could be successfully applied in the management of acute traumatic wounds with tissue loss in dogs. Keywords: canine, moist wound therapy, photobiomodulation therapy, injury, wound healing Journal: Veterinární medicína Pages: 215-220 Volume: 65 Issue: 5 Year: 2020 DOI: 10.17221/131/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/131/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202005-0004.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:5:id:131-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N Lee Author-Workplace-Name: College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea Author-Workplace-Name: Time Animal Medical Center, Dunsan-ro, Seo-gu, Daejeon, Republic of Korea Author-Name: J Seo Author-Workplace-Name: Time Animal Medical Center, Dunsan-ro, Seo-gu, Daejeon, Republic of Korea Author-Name: J Kim Author-Workplace-Name: Time Animal Medical Center, Dunsan-ro, Seo-gu, Daejeon, Republic of Korea Author-Name: J Yoon Author-Workplace-Name: College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea Title: Diagnostic imaging of a mass-shape hepatic steatosis in a dog treated with chemotherapy Abstract: A left medial liver mass was found through the monitoring of the metastasis in a 15-years-old, spayed female Maltese dog. The patient had a history of chemotherapy with carboplatin after surgical resection of the mammary gland tumour. Ultrasonography (US) showed a massive hyperechoic mass in the left liver. The left medial liver mass was identified as a poorly demarcated, hypoattenuated area on the non-enhanced and all the phases of contrast-enhanced computed tomography. The liver mass was surgically removed and the histopathological examination showed a fatty deposition and no evidence of neoplasm. Based on the patient's history, diagnostic imaging and histopathological findings, the liver mass was considered as a chemotherapy-induced hepatic steatosis. This report provides the diagnostic imaging characteristics of the hepatic steatosis that could help differentiate it from a malignancy in a dog treated with chemotherapy. Keywords: chemotherapy, diagnostic imaging, dog, hepatic steatosis, mass shaped Journal: Veterinární medicína Pages: 221-226 Volume: 65 Issue: 5 Year: 2020 DOI: 10.17221/13/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/13/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202005-0005.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:5:id:13-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: HE Kim Author-Workplace-Name: Department of Veterinary Internal Medicine, Konkuk University Veterinary Medical Teaching Hospital, Seoul, Republic of Korea Author-Name: JH Kim Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Title: Successful long-term management with toceranib phosphate of a recurrent muzzle mast cell tumour in a dog Abstract: A 10-year-old spayed female Yorkshire terrier with a muzzle mass was presented. The ulcerated mass was surgically resected, but recurred and grew rapidly over the subsequent 7 months. The submandibular lymph nodes were remarkably enlarged (right: 9.5 × 7.8 cm; left, 4.7 × 4.0 cm). The cytological analysis of the mass and lymph nodes revealed a high-risk mast cell tumour characterised by high mitotic figures (> 5/oil immersion field), nuclear pleomorphism, multi-nucleation, karyomegaly, and anisokaryosis. A polymerase chain reaction analysis targeting the c-kit domain revealed an exon 8 mutation. The resection of the mass was not considered optional because of the location. Oral toceranib (3.25 mg/kg, every other day) and prednisolone (1.0 mg/kg to 0.5 mg/kg, once a day) were administered, and the mass disappeared within 1 month. Subsequently, the toceranib therapy was stopped for 4 months due to neutropenia (0.7 × 109/l). However, the mass recurred and the toceranib was resumed at the original dose, without affecting the neutropenia. No recurrence has been observed at the 16 months' follow up. Despite the persistent neutropenia, no clinical symptoms have presented. This is another report of the successful treatment of a recurrent muzzle MCT with toceranib in a dog. Keywords: canine, c-kit mutation, inoperable, non-resectable, tyrosine kinase inhibitor Journal: Veterinární medicína Pages: 227-232 Volume: 65 Issue: 5 Year: 2020 File-URL: http://vetmed.agriculturejournals.cz/artkey/vet-202005-0006_successful-long-term-management-with-toceranib-phosphate-of-a-8239-recurrent-muzzle-mast-cell-tumour-in-8239.php File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202005-0006.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:5:id:78-2019-VETMED