Template-Type: ReDIF-Article 1.0 Author-Name: L Pena-Avelino Author-Workplace-Name: Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, México Author-Name: I Ceballos-Olvera Author-Workplace-Name: Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, México Author-Name: J Alva-Perez Author-Workplace-Name: Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, México Author-Name: J Vicente Author-Workplace-Name: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, México Author-Name: J Pinos-Rodriguez Author-Workplace-Name: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, México Title: Effects of mesquite (Prosopis laevigata) pods as a potential feed material for kids Abstract: To evaluate the dietary inclusion of mesquite pods (Prosopis laevigata) on the growth performance, rumen fermentation, nitrogen balance, blood metabolites and carcass traits, 15 Creole goat kids (12.1 ± 2.8 kg body weight) were randomly assigned to one of three experimental diets with 0, 300 and 600 g of mesquite pods (dry matter basis). The study lasted 80 days. At the end of this period, the blood and ruminal fluid were sampled and the nitrogen (N) balance was calculated. The growth performance and feed intake were not affected by the mesquite pods. The nitrogen digestibility, N absorbed, and N retained increased linearly (P < 0.05) with the increasing amounts of mesquite pods in the diet. The serum glucose and triglycerides were not affected by the mesquite pods, but the creatinine and uric acid decreased linearly (P < 0.05) as the mesquite pods increased in the diet. The highest (P < 0.05) rumen pH value, ammonia-N concentration, molar proportion of the acetate and the acetate : propionate ratio was found in the goat kids fed the diet with 600 g when compared to those fed diets with 0 and 300 g mesquite pods. Beneficial dietary effects of mesquite pods on the blood metabolites and N retention of the goat kids were found in this study; therefore, the dietary inclusion of mesquite pods at 600 g/kg can be used as a feed alternative for growing goat kids. Keywords: Capra aegagrus hircus, blood metabolites, feed intake, N , excretion, rumen fermentation Journal: Veterinární medicína Pages: 289-296 Volume: 65 Issue: 7 Year: 2020 DOI: 10.17221/106/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/106/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202007-0001.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:7:id:106-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Galan Author-Workplace-Name: Department of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, Spain Author-Name: A Seisdedos-Benzal Author-Workplace-Name: Department of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, Spain Author-Name: BE Carletti Author-Workplace-Name: Small Animal Teaching Hospital, University of Liverpool, Liverpool, United Kingdom Author-Name: S Quiros Author-Workplace-Name: Department of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, Spain Author-Name: EM Martin Author-Workplace-Name: Department of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, Spain Author-Name: D Menor Author-Workplace-Name: Department of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, Spain Author-Name: MM Granados Author-Workplace-Name: Department of Animal Medicine and Surgery, Faculty of Córdoba, University of Córdoba, Córdoba, Spain Title: Cisternal versus lumbar cerebrospinal fluid lactate concentration in healthy dogs Abstract: The analysis of cerebrospinal fluid biomarkers provides a wide range of information about the neurological health of a patient. Lactate is a metabolic precursor necessary for brain gluconeogenesis. When oxidative impairment or mitochondrial damage is present, lactate alteration occurs. The rostro-caudal dynamics of cerebrospinal biomarkers along the craniospinal axis in humans and horses was demonstrated in other studies. To the authors' knowledge, no clinical study has, so far, investigated the cerebrospinal fluid lactate concentration in dogs in association with the puncture site. The purpose of this study was to compare the cerebellomedullary cistern and lumbar cistern cerebrospinal fluid lactate concentrations in healthy dogs. Cerebellomedullary and lumbar cerebrospinal fluids were collected for the cell count, total protein determination and lactate analysis from ten healthy Beagle dogs. The results revealed a significantly increased lumbar cerebrospinal fluid lactate concentration when compared with the cerebellomedullary cistern level. The results included: the total nucleated cell count < 5 cells/µl, the red blood cell count < 500 cell/µl, the total proteins < 0.3 g/l, as well as the cerebellomedullary lactate values (1.44 ± 0.06 mM/l) and the lumbar cistern lactate values (1.58 ± 0.1 mM/l). The results of this study highlight useful data that help to understand the physiological lactate variations depending on the cerebrospinal fluid puncture site. Keywords: brain energy metabolism, canine, puncture, rostro-caudal dynamics Journal: Veterinární medicína Pages: 297-300 Volume: 65 Issue: 7 Year: 2020 DOI: 10.17221/136/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/136/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202007-0002.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:7:id:136-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E Salkova Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic Author-Name: M Flajshans Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic Author-Name: C Steinbach Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic Title: Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus) Abstract: In this study, we describe the immunohistochemical characterisation of the thymus, the main lymphoid organ, in sturgeon. The wide range cytokeratin, vimentin, S-100 protein, LCA (CD45) and CD3 were selected as the immunohistochemical markers to map the thymus in juvenile sterlet (Acipenser ruthenus). The epithelial cells and Hassall's corpuscles were labelled with a wide range cytokeratin. The fibroblasts and connective tissue within the thin fibrous capsule on the thymic surface expressed vimentin positivity. The stromal reticular cells were S-100 protein positive. The Leukocyte Common Antigen LCA (CD45) was negative on the thymic lymphocytes. The CD3 was negative on the thymic lymphocytes with cross-reactivity on the non-targeted structures. In conclusion, the commercially available antibodies against the wide range cytokeratin, vimentin and S-100 protein can be used to differentiate components of the sturgeon thymus, while the LCA (CD45) and CD3 application failed. We suggest that further studies are needed to generate fish specific antibodies. Keywords: CD3, Hassall's corpuscles, LCA (CD45), S-100 protein, vimentin, wide range cytokeratin Journal: Veterinární medicína Pages: 301-308 Volume: 65 Issue: 7 Year: 2020 DOI: 10.17221/181/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/181/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202007-0003.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:7:id:181-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: LPB Borges Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Author-Name: GMA Santos Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Author-Name: RA Oliveira Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Author-Name: LO Silva Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Author-Name: LA de Souza Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Author-Name: HH Perdigao Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Author-Name: MM Guimaraes Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Author-Name: JAS Silveira Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Author-Name: MD Duarte Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Author-Name: PPM Teixeira Author-Workplace-Name: Institute of Veterinary Medicine, Veterinary Hospital, Universit Federal do Pará (UFPA), Castanhal, PA, Brazil Title: Metacarpophalangeal joint luxation with joint capsule rupture and bone exposure in a horse Abstract: A 300 kg, 11-month-old quarter horse filly was presented with a joint dislocation in the right anterior limb. The animal was attended at the Federal University of Pará (UFPA) Veterinary Hospital in September 2016, where it was reported that the horse was injured on a fence. On clinical examination, the dislocation of the metacarpophalangeal joint with a rupture of the joint capsule and exposure of bone surfaces was observed, however, with no rupture of the extensor and flexor tendons. Surgery was recommended and the patient was anaesthetised with detomidine (Dormiun V; Agener União, São Paulo, Brazil; 0.04 mg/kg), ketamine (Cetamin; Syntec, São Paulo, Brazil; 0.03 mg/kg/h) and guaiacol glyceryl ether (JA Saúde Animal, São Paulo, Brasil, 0.5 ml/kg/h) i.v. The reconstitution of the joint capsule, lateral collateral ligament (LCL) and the other soft tissues around the injury was performed. A chemical arthrodesis was performed, then the tissue approximation and finally the skin suture. Bandages were applied on the wound with hydrophobic cotton and a Robert-Jones dressing. Systemic treatment was performed with enrofloxacin (Zelotril 10%; Agener União, São Paulo, Brazil; 5 mg/kg) i.m., meloxicam (Maxicam 2%; São Paulo, Brasil; 0.6 mg/kg) i.v. during 10 and 20 days, respectively, then replacing the antibiotic with ceftiofur (Cef 50; Agener União, São Paulo, Brasil; 2.2 mg/kg) i.m. for another 22 days. An iodine solution was applied to the joint optimising arthrodesis. After three and a half months, the lesion was entirely healed and with good movement, so the patient was discharged. This case demonstrates that, with adequate treatment and attention, it is possible to recover the patient's quality of life. Keywords: chemical arthrodesis, equine, joint reconstruction, lateral collateral ligament, injury Journal: Veterinární medicína Pages: 309-313 Volume: 65 Issue: 7 Year: 2020 DOI: 10.17221/170/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/170/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202007-0004.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:7:id:170-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: T Plavec Author-Workplace-Name: Small Animal Veterinary Hospital Hofheim, Hofheim, Germany Author-Workplace-Name: Small Animal Clinic, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Author-Name: T Svara Author-Workplace-Name: Institute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Author-Name: N Tozon Author-Workplace-Name: Small Animal Clinic, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Author-Name: D Pavlin Author-Workplace-Name: Small Animal Clinic, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Title: Spontaneous rupture of the lymph nodes as a cause of haemoabdomen in two canine lymphoma patients Abstract: Non-traumatic haemoabdomen in dogs usually occurs due to abdominal neoplasia, coagulopathies or organ torsion. The most common sources of bleeding in neoplastic cases are the spleen and liver, but other abdominal organs can also be involved. However, in the available veterinary literature, ruptured lymph nodes are not described as a cause of haemoabdomen. In the present manuscript, two canine cases of intra-abdominal haemorrhage from ruptured lymph nodes secondary to B-cell lymphoma are described. Keywords: dog, haemoperitoneum, multicentric lymphoma, neoplasia, nontraumatic haemoabdomen Journal: Veterinární medicína Pages: 314-319 Volume: 65 Issue: 7 Year: 2020 DOI: 10.17221/21/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/21/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202007-0005.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:7:id:21-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S Park Author-Workplace-Name: Veterinary Medical Imaging, College of Veterinary Medicine and BK 21 Plus Project Team, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju, Republic of Korea Author-Name: MJC Ang Author-Workplace-Name: Laboratory of Veterinary Pathology, College of Veterinary Medicine and BK 21 Plus Project Team, College of Veterinary Medicine, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju, Republic of Korea Author-Name: MD Kittleson Author-Workplace-Name: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California Davis, Davis, CA Author-Name: KO Cho Author-Workplace-Name: Laboratory of Veterinary Pathology, College of Veterinary Medicine and BK 21 Plus Project Team, College of Veterinary Medicine, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju, Republic of Korea Author-Name: J Choi Author-Workplace-Name: Veterinary Medical Imaging, College of Veterinary Medicine and BK 21 Plus Project Team, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju, Republic of Korea Title: Tetralogy of Fallot with right ventricular outflow tract obstruction and patent ductus arteriosus in a dog Abstract: In this study we describe the echocardiographic features of a complex cardiac defect with a right to left shunt through tetralogy of Fallot and also a left to right shunt through a patent ductus arteriosus (PDA) in a 3-year-old dog. The echocardiography revealed a tetralogy of Fallot with a severely hypertrophied right ventricular outflow tract (RVOT), and a left to right shunting PDA. A contrast echocardiogram showed microbubbles moving from the right ventricle to the aorta through the ventricular septal defect. They then reached the main pulmonary artery through the PDA rather than through the RVOT. The necropsy confirmed tetralogy of Fallot with an RVOT obstruction and a PDA. This patient could have maintained the pulmonary circulation through the PDA in the spite of the right ventricular outflow tract obstruction and survive a long period. Not only the echocardiography, but also the contrast echocardiogram using agitated saline and trans-sectional images in CT enhanced the comprehensive understanding of the anatomic defects in this complex cardiac defect. Keywords: canine, computed tomography, contrast echocardiogram Journal: Veterinární medicína Pages: 320-326 Volume: 65 Issue: 7 Year: 2020 DOI: 10.17221/76/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/76/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202007-0006.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:7:id:76-2019-VETMED