Template-Type: ReDIF-Article 1.0 Author-Name: D Sperling Author-Workplace-Name: Ceva Santé Animale, Libourne, France Author-Name: H Karembe Author-Workplace-Name: Ceva Santé Animale, Libourne, France Author-Name: M Zouharova Author-Workplace-Name: Derpartment of Immunology, Veterinary Research Institute, Brno, Czech Republic Author-Name: K Nedbalcova Author-Workplace-Name: Derpartment of Immunology, Veterinary Research Institute, Brno, Czech Republic Title: Examination of the minimum inhibitory concentration of amoxicillin and marbofloxacin against Streptococcus suis using standardised methods Abstract: The results of the antimicrobial susceptibility testing of clinical isolates Streptococcus suis to amoxicillin and marbofloxacin obtained by the agar dilution method and broth microdilution method with the results obtained by the commercially available E-test were compared. Comparisons between the methods based on the determination of the minimal inhibitory concentration (MIC) of the antimicrobials were assessed based on the degree and frequency of the categorical agreement (Agar dilution method as a reference system) and the percentage of the categorical agreement and error rate. A statistical evaluation was determined using the Bland-Atman method. The presented MIC values, determined for the isolates in the E-test, were slightly different from the MIC values determined by the dilution tests, mainly due to the different defined testing concentrations. For the E-test as the test system and agar-dilution method as the reference system, no error of any class was detected (very major, major and minor error) and a complete categorical agreement was obtained between the evaluated methods for amoxicillin. For amoxicillin, the regression and correlation analysis show linear relationships between the E-test and the two dilution methods with significant coefficients of determination (0.62 and 0.75). The slopes of the equality and regression lines were not significantly different. However, the E-test tends to slightly overestimate the MIC values when compared to the microdilution. The reverse is true when compared with the agar dilution. There was good agreement between the E-test and the dilution methods with a low bias (0.001 3 and -0.005 0), all the experimental data were within the computed limits of agreement. For marbofloxacin, the same trends were observed with lower coefficients of determination (0.42 and 0.73) and a less favourable agreement. The E-test constantly underestimated the MIC values when compared to the two dilution methods. No significant difference between the microdilution and agar dilution was obtained. Keywords: antimicrobial, resistance, agar dilution, microdilution, E-test Journal: Veterinární medicína Pages: 377-386 Volume: 65 Issue: 9 Year: 2020 DOI: 10.17221/111/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/111/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202009-0001.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:9:id:111-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: X Li Author-Workplace-Name: College of Life Science, Longyan University, Longyan, Fujian, P.R. China Author-Workplace-Name: Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian, P.R. China Author-Name: L Qiu Author-Workplace-Name: College of Life Science, Longyan University, Longyan, Fujian, P.R. China Author-Workplace-Name: Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian, P.R. China Author-Name: GH Qiu Author-Workplace-Name: College of Life Science, Longyan University, Longyan, Fujian, P.R. China Author-Workplace-Name: Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian, P.R. China Author-Name: X Yang Author-Workplace-Name: College of Life Science, Longyan University, Longyan, Fujian, P.R. China Author-Workplace-Name: Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian, P.R. China Author-Name: X Zheng Author-Workplace-Name: College of Life Science, Longyan University, Longyan, Fujian, P.R. China Author-Workplace-Name: Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian, P.R. China Title: Evaluation of red clover isoflavone extract as a vaccine adjuvant for piglets against Haemophilus parasuis Abstract: Glässer's disease of swine caused by Haemophilus parasuis (H. parasuis) is one of the major bacterial diseases affecting pig farms worldwide. Vaccination is a crucial measure for controlling the H. parasuis infection. Adjuvants are employed to enhance the immunity effects of inactivated vaccines or subunit vaccines. In the present study, a red clover isoflavone extract (RCIE) was investigated as an adjuvant for the H. parasuis inactivated vaccine. Thirty colostrum-deprived (CD) piglets (mixed-breed: Large White × Landrace) aged 15 days were vaccinated on days 0 and 14 with an inactivated H. parasuis vaccine with or without an adjuvant. The adjuvant groups' vaccines were mixed with a high-dose RCIE (20 mg/ml), a middle-dose RCIE (10 mg/ml), a low-dose RCIE (5 mg/ml), or with Montanide Gel 01 (10%, v/v). Phosphate buffer saline (PBS) was also given as a blank control. Fourteen days after the booster immunisation, the piglets were challenged with H. parasuis LY02 (serotype 5). The IgG antibody, cytokines, T lymphocyte subpopulations, and clinical and pathological signs of the piglets were evaluated. The results showed that the RCIE enhanced the H. parasuis vaccine and elicited strong antibody levels as well as the cytokines IL-2, IL-4, and IFN-γ in serum, and the levels depended on the RCIE dose. Moreover, the piglets vaccinated with the inactivated LY02 containing the Middle-dose RCIE had a higher survival rate in the challenge experiments. In conclusion, RCIE can enhance the H. parasuis vaccine immunity by promoting titres of IgG antibody and by improving the Th1-type cellular immunity. Keywords: adjuvant, Haemophilus parasuis, red clover isoflavone extract, vaccine, immunity Journal: Veterinární medicína Pages: 387-393 Volume: 65 Issue: 9 Year: 2020 DOI: 10.17221/44/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/44/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202009-0002.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:9:id:44-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Zupcic Author-Workplace-Name: Student (6th year) at Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M Surbek Author-Workplace-Name: Student (6th year) at Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: A Ekert Kabalin Author-Workplace-Name: Department of Animal Breeding and Livestock Production, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: J Dragovic Author-Workplace-Name: SUZA - Alliance of Animal Protection Associations of the City of Zagreb, Zagreb, Croatia Author-Name: S Mencik Author-Workplace-Name: Department of Animal Breeding and Livestock Production, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M Ostovic Author-Workplace-Name: Department of Animal Hygiene, Behaviour and Welfare, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Missing cat features influencing their returning to owners Abstract: In this study, the features influencing finding and returning missing cats to their owners were investigated. The data were collected on the missing and recovered cats in the City of Zagreb, Croatia, during the 2011-2016 period, including the following features: breed, sex, age, colour, hair length, castration and microchipping. A data analysis was performed by use of the Statistica v13.4 software. Out of 946 cats reported to be missing, 372 (39%) were returned to their owners. Purebred (P < 0.01), castrated (P < 0.05), microchipped (P < 0.05) cats, and those with semi-long and long hair (P < 0.05) were more frequently returned to their owners, whereas the sex, age and colour had no significant impact on the missing cat recovery. The study results showed particular cat features influencing their finding to be correlated (P < 0.05). In conclusion, cat owners should attempt to control their movements, in particular of older and non-castrated cats, to reduce the rate of cats going missing. In addition, microchipping would facilitate finding them. The large-scale implementation of the concepts highlighted in this study can contribute to reducing the number of missing cats and increasing the number of cats being returned to their owners. Keywords: missing cat, found cat, microchipping, castration, breed, hair length Journal: Veterinární medicína Pages: 394-400 Volume: 65 Issue: 9 Year: 2020 DOI: 10.17221/185/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/185/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202009-0003.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:9:id:185-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P Martz Author-Workplace-Name: Clinic for Ruminants and Swine, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany Author-Name: A Oezcan-Martz Author-Workplace-Name: Clinic for Ruminants and Swine, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany Author-Name: L Bittner Author-Workplace-Name: Clinic for Ruminants and Swine, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany Author-Name: F Ebert Author-Workplace-Name: Clinic for Ruminants and Swine, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany Author-Name: W Wippermann Author-Workplace-Name: Clinic for Ruminants and Swine, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany Author-Name: A Woeckel Author-Workplace-Name: Clinic for Ruminants and Swine, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany Author-Name: D Boettcher Author-Workplace-Name: Institute of Veterinary Pathology, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany Author-Name: W Baumgartner Author-Workplace-Name: University Clinic for Ruminants, University of Veterinary Medicine Vienna, Vienna, Austria Author-Name: A Starke Author-Workplace-Name: Clinic for Ruminants and Swine, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany Title: Case reports of genital tract tumours in cows Abstract: The goal of this report is to analyse the incidence of genital tumours in cattle referred to the Clinic between 2016 and 2019 and to describe the outcome after the surgical tumour resection. All cattle with a confirmed diagnosis of a genital tumour (n = 3) were included in the study. The breed, age, tumour localisation and type of treatment were considered. Telephone interviews were used to ask owners about the integration of the cows into the herd after discharge, the survival, the reason for leaving the herd, wound complications and tumour recurrence. Three genital tumours including a vaginal fibrosarcoma, vaginal leiomyosarcoma and luteoma were diagnosed. A tumour recurrence or metastasis did not occur. In conclusion, the tumour resection was successful in all three cases. To our knowledge, this is the first clinical description of a cow with a luteoma. Keywords: neoplasm, mass, cow, reproductive tract, ovary Journal: Veterinární medicína Pages: 401-408 Volume: 65 Issue: 9 Year: 2020 DOI: 10.17221/124/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/124/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202009-0004.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:9:id:124-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I Butkovic Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: S Vince Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: K Marjanovic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: I Folnozic Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: N Macesic Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: B Spoljaric Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: J Savoric Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M Samardzija Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M Lojkic Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: D Djuricic Author-Workplace-Name: Veterinary Practice Djurdjevac, Djurdjevac, Croatia Author-Name: G Bacic Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: T Karadjole Author-Workplace-Name: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Laparoscopic ovariectomy in a pygmy goat Abstract: The aim of this study was to describe a laparoscopic ovariectomy in a pygmy goat. This minimally invasive surgical technique was performed for the first time on this type of animal at the Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Zagreb. The owner requested this surgical procedure because of its advantages, such as the reduction of the incision size, the duration of surgery, the improved visualisation of the surgical site, the minimum extent of the anatomical approach and the reduction in any trauma, pain and postoperative complications including improved cicatrisation. Keywords: endoscopic surgery, small ruminants, spaying Journal: Veterinární medicína Pages: 409-414 Volume: 65 Issue: 9 Year: 2020 DOI: 10.17221/113/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/113/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202009-0005.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:9:id:113-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: REM Oliveira Author-Workplace-Name: Postgraduate Program in Animal Science, Department of Animal Sciences, Federal Rural University of Semi-Árido - UFERSA, Mossoró-RN, Brazil Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Vertebrate Morphophysiology Laboratory, Department of Morphology, Bioscience Center - CB, Federal University of Rio Grande do Norte - UFRN, Natal-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Name: JML Pires Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Name: JS Batista Author-Workplace-Name: Postgraduate Program in Animal Science, Department of Animal Sciences, Federal Rural University of Semi-Árido - UFERSA, Mossoró-RN, Brazil Author-Name: FLN Attademo Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Name: DSD Farias Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Vertebrate Morphophysiology Laboratory, Department of Morphology, Bioscience Center - CB, Federal University of Rio Grande do Norte - UFRN, Natal-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Name: ACB Freire Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Name: AC Bomfim Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Vertebrate Morphophysiology Laboratory, Department of Morphology, Bioscience Center - CB, Federal University of Rio Grande do Norte - UFRN, Natal-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Name: LRP Lima Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Vertebrate Morphophysiology Laboratory, Department of Morphology, Bioscience Center - CB, Federal University of Rio Grande do Norte - UFRN, Natal-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Name: RM Oliveira Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Workplace-Name: Postgraduate Program in Natural Sciences, Faculty of Exact and Natural Sciences, State University of Rio Grande do Norte -UERN, Mossoró-RN, Brazil Author-Name: SA Gavilan Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Vertebrate Morphophysiology Laboratory, Department of Morphology, Bioscience Center - CB, Federal University of Rio Grande do Norte - UFRN, Natal-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Name: FJL Silva Author-Workplace-Name: Cetáceos Costa Branca Project, State University of Rio Grande do Norte - PCCB-UERN, Mossoró-RN, Brazil Author-Workplace-Name: Vertebrate Morphophysiology Laboratory, Department of Morphology, Bioscience Center - CB, Federal University of Rio Grande do Norte - UFRN, Natal-RN, Brazil Author-Workplace-Name: Center for Environmental Studies and Monitoring - CEMAM, Areia Branca -RN, Brazil Author-Name: MF Oliveira Author-Workplace-Name: Postgraduate Program in Animal Science, Department of Animal Sciences, Federal Rural University of Semi-Árido - UFERSA, Mossoró-RN, Brazil Title: Death of a loggerhead sea turtle (Caretta caretta) from ingestion of an eel (Myrichthys ocellatus) Abstract: Gastrointestinal perforation in sea turtles may be associated with the ingestion of solid waste; however, other factors, including the ingestion of marine organisms, may cause intestinal perforation. Herein, we report, presumably, the first case of a death of a loggerhead sea turtle (Caretta caretta) from ingesting a live goldspotted eel (Myrichthys ocellatus) and describe the necropsy findings. The adult female loggerhead sea turtle was registered alive by a team of the Cetáceos da Costa Branca Project of Rio Grande do Norte State University (PCCB-UERN). The animal died in captivity after 8 days of an attempted rehabilitation process, and the carcass was immediately sent for necropsy. After incision, an encapsulated structure in the cranial region of the left lung was identified as a specimen of the ingested eel. The histopathological examination of the lung showed fibrin and numerous leukocytes, mainly macrophages, in the alveolus and bronchioles. The necropsy revealed that the ingestion of a live goldspotted eel (M. ocellatus) caused a gastric perforation in the turtle; this resulted in the displacement of the eel to the celomatic cavity with its cranial portion in the left lung, leading to sepsis and the consequent death of the turtle. Keywords: Cheloniidae, gastric perforation, septicaemia, Testudines Journal: Veterinární medicína Pages: 415-420 Volume: 65 Issue: 9 Year: 2020 DOI: 10.17221/83/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/83/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202009-0006.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:9:id:83-2020-VETMED