Template-Type: ReDIF-Article 1.0 Author-Name: C Perez-Martinez Author-Workplace-Name: Department of Animal Health, Section of Pathology, Veterinary School, University of León, León, Spain Author-Workplace-Name: Institute of Biomedicine (IBIOMED), University of León, León, Spain Author-Name: A Perez de Prado Author-Workplace-Name: Institute of Biomedicine (IBIOMED), University of León, León, Spain Author-Workplace-Name: Health Research Foundation in León, León, Spain Author-Name: V Caballero-Manso Author-Workplace-Name: Department of Animal Health, Section of Pathology, Veterinary School, University of León, León, Spain Author-Name: M Regueiro-Purrinos Author-Workplace-Name: Institute of Biomedicine (IBIOMED), University of León, León, Spain Author-Workplace-Name: Department of Veterinary Medicine, Surgery and Anatomy, Section of Surgery, Veterinary School, University of León, León, Spain Author-Name: Garcia MG de Garnica Author-Workplace-Name: MicrosVeterinaria SL, León, Spain Author-Name: C Cuellas-Ramon Author-Workplace-Name: Institute of Biomedicine (IBIOMED), University of León, León, Spain Author-Workplace-Name: Health Research Foundation in León, León, Spain Author-Name: JM Gonzalo-Orden Author-Workplace-Name: Institute of Biomedicine (IBIOMED), University of León, León, Spain Author-Workplace-Name: Department of Veterinary Medicine, Surgery and Anatomy, Section of Surgery, Veterinary School, University of León, León, Spain Author-Name: M Lopez-Benito Author-Workplace-Name: Institute of Biomedicine (IBIOMED), University of León, León, Spain Author-Workplace-Name: Health Research Foundation in León, León, Spain Author-Name: JR Altonaga Author-Workplace-Name: Institute of Biomedicine (IBIOMED), University of León, León, Spain Author-Workplace-Name: Department of Veterinary Medicine, Surgery and Anatomy, Section of Surgery, Veterinary School, University of León, León, Spain Author-Name: T Benito-Gomez Author-Workplace-Name: Institute of Biomedicine (IBIOMED), University of León, León, Spain Author-Workplace-Name: Health Research Foundation in León, León, Spain Author-Name: F Fernandez-Vazquez Author-Workplace-Name: Institute of Biomedicine (IBIOMED), University of León, León, Spain Author-Workplace-Name: Health Research Foundation in León, León, Spain Title: Adventitial histopathological changes after coronary stenting in a porcine model Abstract: The adverse long-term events in first-generation drug-eluting stents were associated with chronic inflammatory response to the polymer. As an alternative, stents with biodegradable polymers emerged, whose effects on the vascular response are not yet fully known. Our objectives were to study the adventitial response to the stent implantation and the role of the polymeric vehicle. A histological (Haematoxylin-Eosin, Verhoeff van Gieson) and immunohistochemical (von Willebrand factor, alpha-smooth muscle actin) analysis were performed on resin-embedded arterial sections from fifteen Large White pigs, 28 days after the random implantation in the coronary arteries of: a chromium-cobalt stent and a stent coated with a permanent polyacrylate or biodegradable poly(D,L)-lactic-co-glycolic polymer, the two latter ones are loaded with sirolimus. Independent of the stent, the adventitial inflammation was associated with the adventitial area (P = 0.006 8) and the inflammation score (P = 0.037 1); and the adventitial actin-positive cells with the vascular damage (P = 0.001 2). A significant relationship was observed between the greater percentages of the restenosis and the more intense inflammation (P = 0.035 1) and the actin-positive cells (P = 0.011 9) in the adventitia. The polymeric vehicle increased the adventitial actin-positive cells (P = 0.018), independent of the type of polymer. The adventitial changes seem to be related to the restenosic process 28 days after the coronary stenting. Further investigations are necessary to confirm the role of the polymeric vehicle on the adventitial histopathological changes. Keywords: adventitia, pig, polymer, restenosis, stent Journal: Veterinární medicína Pages: 465-472 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/159/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/159/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202011-0001.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:11:id:159-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H Jankowiak Author-Workplace-Name: Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland Author-Name: P Balogh Author-Workplace-Name: Department of Research Methodology and Statistics, University of Debrecen, Hungary Author-Name: A Cebulska Author-Workplace-Name: Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland Author-Name: E Vaclavkova Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves/Kostelec nad Orlicí, Czech Republic Author-Name: M Bocian Author-Workplace-Name: Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland Author-Name: P Reszka Author-Workplace-Name: Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Bydgoszcz, Poland Title: Impact of piglet birth weight on later rearing performance Abstract: The piglet birth weight and its variance within a litter may be considered important traits that influence pig productivity in the first and later stages of rearing. 222 piglets were evaluated from the moment they were born until weaning, and then as fattening pigs until the end of the fattening (n = 207) in three birth weight groups. Negative consequences of an excessively low piglet birth weight were observed, including higher mortality until weaning and a lower average daily gain during suckling. The correlation coefficients between the piglet birth weight and the remaining indicators confirmed the negative impact of the low piglet birth weight, fattening performance and carcass slaughter value (P < 0.01). The regression analysis between the piglet birth weight and the growth rate during the whole rearing phases indicated that only the piglet growth rate from birth to weaning is determined by their birth weight. Keywords: pigs, body weight at , birth, fattening, slaughter traits Journal: Veterinární medicína Pages: 473-479 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/117/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/117/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202011-0002.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:11:id:117-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B Savic Author-Workplace-Name: Department for Pathology, Institute of Veterinary Medicine Belgrade, Belgrade, Republic of Serbia Author-Workplace-Name: Department for Veterinary Medicine, Agriculture Faculty, University of Novi Sad, Novi Sad, Republic of Serbia Author-Name: O Radanovic Author-Workplace-Name: Department for Pathology, Institute of Veterinary Medicine Belgrade, Belgrade, Republic of Serbia Author-Name: V Milicevic Author-Workplace-Name: Department for Pathology, Institute of Veterinary Medicine Belgrade, Belgrade, Republic of Serbia Author-Name: B Kureljusic Author-Workplace-Name: Department for Pathology, Institute of Veterinary Medicine Belgrade, Belgrade, Republic of Serbia Author-Name: N Zdravkovic Author-Workplace-Name: Department for Pathology, Institute of Veterinary Medicine Belgrade, Belgrade, Republic of Serbia Author-Name: K Nesic Author-Workplace-Name: Department for Pathology, Institute of Veterinary Medicine Belgrade, Belgrade, Republic of Serbia Author-Name: O Stevancevic Author-Workplace-Name: Department for Veterinary Medicine, Agriculture Faculty, University of Novi Sad, Novi Sad, Republic of Serbia Title: Distribution and aetiology of valvular endocarditis in suckling and weaned piglets in Serbia Abstract: There are limited data available in the literature about the frequency and distribution of endocarditis in suckling and weaned piglets. The goal of this study was to investigate the frequency and distribution of lesions from cases of spontaneous valvular endocarditis in young piglets and to determine the bacterial agents involved in those lesions. Valvular endocarditis lesions in suckling piglets were detected in 21/454 cases (4.62%) and in 20/306 cases (6.53%) in weaned piglets. Streptococci were the dominant bacterial species isolated in 68% of the cases; S. suis was identified in 48.7% of the cases, S. pyogenes in 7.3% and S. spp., which could not be further identified, were cultured in 12.1% of the cases. E. rhusiopathiae was identified as the causal agent of endocarditis in four cases in the weaned piglets. In three cases, A. suis and S. aureus were isolated, and, in two cases, A. pleuropneumoniae was identified. Y. enterocolitica was isolated form one case of valvular endocarditis in a weaned pig. The finding of valvular endocarditis caused by Y. enterocolitica is an uncommon finding in pigs, and to the authors' knowledge, there are no other reports of this agent being the cause of endocarditis in pigs. The involvement of Y. enterocolitica in endocarditis might have been assisted by the predisposing role of immunosuppressive viruses, such as porcine reproductive and respiratory syndrome. Keywords: swine, piglets, endocarditis, bacteria Journal: Veterinární medicína Pages: 480-486 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/99/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/99/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202011-0003.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:11:id:99-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P Mikula Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: J Blahova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: A Honzlova Author-Workplace-Name: State Veterinary Institute Jihlava, Horni Kosov, Jihlava, Czech Republic Author-Name: J Kalinova Author-Workplace-Name: Central Institute for Supervising and Testing in Agriculture, Brno, Czech Republic Author-Name: P Macharackova Author-Workplace-Name: State Veterinary Institute Olomouc, Olomouc, Czech Republic Author-Name: J Rosmus Author-Workplace-Name: State Veterinary Institute Prague, Prague-Lysolaje, Czech Republic Author-Name: Z Svobodova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Author-Name: M Svoboda Author-Workplace-Name: Ruminant and Swine Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic Title: Occurrence of mycotoxins in complete poultry feeds in the Czech Republic - Multiannual survey (2013-2018) Abstract: To assess the contamination and potential health risks for poultry, we investigated concentrations of selected mycotoxins in complete feeds sampled within the period of 2013-2018 in the Czech Republic. Broiler feeds, feeds for laying hens, chickens and/or other poultry species were investigated by ELISA methods or chromatography. Our results demonstrated that unlike other mycotoxins, (such as aflatoxins, ochratoxin A or zearalenone), deoxynivalenol (DON) may represent a potential threat. The prevalence of this mycotoxin in all the feed categories was very high, always reaching at least 81.29%. Moreover, in 14 of the 139 analysed broiler feed samples (i.e., in 10.81% of samples), DON was detected in concentrations exceeding the recent guidance value of 5 000 µg/kg established by EU legislation. Since previous studies demonstrated that the long-term feeding of such a highly contaminated diet could cause stress in the animals and could lead to their growth suppression, good agricultural practices and the further systematic monitoring of Czech poultry feeds are still highly needed. Keywords: broilers, deoxynivalenol, feed contamination, filamentous fungi, Fusarium, mycotoxin Journal: Veterinární medicína Pages: 487-494 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/109/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/109/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202011-0004.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:11:id:109-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Abidi Author-Workplace-Name: Faculty of Sciences of Tunis, University of Tunis El Manar, Manar II Tunis, Tunisia Author-Workplace-Name: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia Author-Name: E Sebai Author-Workplace-Name: Faculty of Sciences of Tunis, University of Tunis El Manar, Manar II Tunis, Tunisia Author-Workplace-Name: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia Author-Name: M Dhibi Author-Workplace-Name: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia Author-Name: MA Darghouth Author-Workplace-Name: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia Author-Name: H Akkari Author-Workplace-Name: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia Title: Chemical analyses and evaluation of the anthelmintic effects of Origanum majorana essential oil, in vitro and in vivo studies Abstract: Because of the development of resistance in helminths against major anthelmintic drugs, the search for alternatives is necessary. Medicinal plants are being studied as an alternative source of anthelmintics against gastrointestinal nematodes. The objective of this study is to analyse the chemical composition and evaluate the anthelmintic efficacy of Origanum majorana essential oil. The determination of the chemical composition by gas chromatography/mass chromatography (GC/MS) revealed that the essential oil was dominated by terpenoids, particularly carvacrol (35.65%) and terpenic hydrocarbons p-cymene (15.82%). The in vitro anthelmintic effects against Haemonchus contortus were assessed by an egg hatch assay (EHA) and an adult worm motility assay (AWMA) compared with the reference drug albendazole. The essential oil showed ovicidal activity at all the tested concentrations (1, 2, 4 and 8 mg/ml) and more than 80% egg hatching inhibition was observed at the highest dose (8 mg/ml). Exposure to 0.5 mg/ml of the essential oil for eight hours induced a 50% inhibition in the worm motility. The in vivo study was performed on H. polygyrus by measuring the egg count reduction (ECR) and adult worm count reduction (AWCR) following the treatment of the animals with different doses (2 000, 4 000 and 5 000 mg/kg) of the plant essential oil, and 22 mg/kg of albendazole as the positive control. The results showed that 5 000 mg/kg of the essential oil inhibited the egg count and adult worm count by 76.3 and 74.0%, respectively, seven days post treatment. These findings support the possible use of O. majorana essential oil to control gastrointestinal nematodes. Keywords: fresh herb, Haemonchus contortus, Heligmosomoides polygyrus, anthelmintic activity, faecal egg count, GC/MS analysis Journal: Veterinární medicína Pages: 495-505 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/115/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/115/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202011-0005.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:11:id:115-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K Lee Author-Workplace-Name: Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea Author-Name: H Kim Author-Workplace-Name: Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea Author-Name: J Sohn Author-Workplace-Name: Veterinary Service Laboratory, Daegu, Republic of Korea Author-Name: BJ So Author-Workplace-Name: Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea Author-Name: EJ Choi Author-Workplace-Name: Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea Title: Systemic mucormycosis caused by Lichtheimia ramosa in a pregnant cow Abstract: Mucormycosis is a life-threatening fungal disease caused by opportunistic pathogens present in the environment. This report presents a case of a Lichtheimia ramosa infection that manifested as characteristic lesions in various visceral organs of a pregnant cow in Korea. The post-mortem examination revealed the cause of death to be mucormycosis. The fungi isolated from the liver were found to be Lichtheimia spp. Analysis of the fungi using Internal Transcribed Spacer gene sequencing facilitated the identification of Lichtheimia ramosa. To the best of our knowledge, this is the first clinical case of angioinvasive mucormycosis in cattle. Keywords: cattle, fungal disease, angiovasive, Internal Transcribed Spacer Journal: Veterinární medicína Pages: 506-510 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/8/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202011-0006.txt Handle: RePEc:caa:jnlvet:v:65:y:2020:i:11:id:8-2020-VETMED