Template-Type: ReDIF-Article 1.0 Author-Name: V Fichtelova Author-Workplace-Name: Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: A Kralova Author-Workplace-Name: Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: P Fleischer Author-Workplace-Name: Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: V Babak Author-Workplace-Name: Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic Author-Name: K Kovarcik Author-Workplace-Name: Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Title: Detection of Mycobacterium avium subspecies paratuberculosis in environmental samples from infected Czech dairy herds Abstract: The objective of the present study was to evaluate the suitability of environmental sampling to screen Czech dairy herds to detect Mycobacterium avium ssp. paratuberculosis (MAP) and to find the most convenient location for the MAP detection in the lactating cow area. Environmental samples (ES, n = 72) from milking parlour holding pens (n = 19), milking alleyways (n = 19) and free-stall alleyways (n = 34) from 19 herds were simultaneously tested to detect MAP by a quantitative PCR (qPCR) and bacterial culture. Eight and thirteen samples from the milking parlour holding pens, twelve and eleven samples from the milking alleyways and eleven and eighteen samples from the free-stall alleyways were qPCR and culture positive, respectively. A 4.6 times higher probability of being culture positive than qPCR positive was detected for the assessable MAP detection results from the free-stall alleyways [P = 0.008 6, odds ration (OR) = 4.572 8)] and no association was found between the results from the milking parlour holding pens (P = 0.191 4) and the milking alleyways (P > 0.999 9) and the diagnostic method used. The percentage of qPCR-positive samples in the tested locations was detected for the milking alleyways (63.2%), free-stall alleyways and milking parlour holding pens. The herd infectious status was in agreement with 16 (84.2%), 14 (73.7%) and 12 (63.2%) qPCR results from the milking alleyways, free-stall alleyways (32.4%) and milking parlour holding pens (42.1%), respectively. No statistically significant differences were detected for these results (P = 0.396 1). MAP was detected by the qPCR and bacterial culture in all three locations where the ES were collected. We suggest an environmental sampling followed by MAP detection by qPCR as an easy-to-perform time-saving protocol for MAP screening in Czech dairy herds. Although the milking alleyways seem to be the most convenient location for the environmental sampling, this assumption was not statistically supported. Keywords: Johne's disease, lactating cow, sampling location, faecal culture, quantitative PCR Journal: Veterinární medicína Pages: 1-7 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/168/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/168/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202101-0001.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:1:id:168-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: MC de Souza Author-Workplace-Name: Department of Veterinary Medicine, State University of Londrina, Londrina, PR, Brazil Author-Name: JR Calesso Author-Workplace-Name: Department of Clinic and Surgery Veterinary, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil Author-Name: B Cenci Author-Workplace-Name: Department of Veterinary Medicine, State University of Londrina, Londrina, PR, Brazil Author-Name: MJL Cardoso Author-Workplace-Name: Department of Veterinary Medicine, State University of Londrina, Londrina, PR, Brazil Author-Name: FA Moura Author-Workplace-Name: Sport Sciences Department, Laboratory of Applied Biomechanics, State University of Londrina, Londrina, PR, Brazil Author-Name: R Fagnani Author-Workplace-Name: Department of Veterinary Medicine, University of Northern Paraná, Londrina, PR, Brazil Title: Kinematics of healthy American Pit Bull Terrier dogs Abstract: A visual clinical gait analysis is useful, however, it may overlook small, but important, details about the movement, as well as differences between the normal and pathological locomotion. The branch of mechanics that describes the spatial and temporal components of motion is called kinematics, providing quantitative data regarding linear and angular motion. The objective of this study was to establish kinematic gait data of healthy American Pit Bull Terriers and to contribute to the understanding of the locomotion. We evaluated the articular and pelvic angles, and the spatiotemporal variables for walking and trotting from eleven dogs with no previous history of joint and musculoskeletal diseases. Twenty reflective markers were positioned at the anatomical points of interest. The animals walked and trotted in a linear space, led by the same researcher. The kinematic data were collected through optoelectronic cameras and analysed by motion analysis software. The movements analysed during the gait phases were the flexion, extension, range of motion (ROM), angle at the moment of the support phase, stride length and velocity. Comparing the angles between walking and trotting, there were more expressive differences for the pelvic limb joints. There was no difference between the left and right sides at all of the joint angles of the pelvic limbs during walking and trotting. Therefore, the movement of the pelvic limb is symmetrical in both trotting and walking. Our results present reference values for healthy American Pit Bull Terriers, having clinical relevance for studies of dogs with musculoskeletal diseases. Keywords: canine gait, joint angles, kinetics, musculoskeletal system Journal: Veterinární medicína Pages: 8-16 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/178/2019-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/178/2019-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202101-0002.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:1:id:178-2019-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B Vojtek Author-Workplace-Name: Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: J Mojzisova Author-Workplace-Name: Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: L Kulichova Author-Workplace-Name: Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: P Smrco Author-Workplace-Name: Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: M Drazovska Author-Workplace-Name: Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Title: Effects of dietary nucleotides and cationic peptides on vaccination response in cats Abstract: Modulation of the immune system through nutrition is the aim of many studies. As the induction of a rapid onset of immunity in cats is often critical, the objective of this study was to evaluate the effects of orally administered dietary nucleotides and cationic peptides, on the vaccination response in cats. The cats were allocated to two groups: Group A (n = 8) was fed a diet with dietary nucleotides and cationic peptides for ninety-two days, and Group C (n = 8) was fed a diet without the nucleotides and cationic peptides. The cats were vaccinated against feline herpesvirus 1 (FHV-1), feline calicivirus (FCV) and feline panleukopenia virus (FPV). The proliferation activity of lymphocytes and antibody response after vaccination were evaluated using ELISA kits. Comparing the two groups, a significant increase in the proliferation activity of the lymphocytes (P < 0.01) was observed in Group A, as well as a significant increase in the antibody titres after vaccination against feline herpesvirus (P < 0.05; P < 0.001), feline calicivirus (P < 0.01; P < 0.001) and feline panleukopenia virus (P < 0.01; P < 0.001). Protective levels of the antibodies were reached significantly earlier in Group A than in Group C. In conclusion, dietary nucleotides and cationic peptides have a positive effect on the lymphocyte proliferation and antibody production after vaccination against FHV-1, FCV and FPV in cats. Therefore, we assume that they can be used as a suitable immunostimulatory substance in feline practice. Keywords: feline calicivirus, feline herpesvirus, feline panleukopenia virus, fed supplementation, immunity Journal: Veterinární medicína Pages: 17-23 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/35/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/35/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202101-0003.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:1:id:35-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H Gaspar Author-Workplace-Name: Animal and Veterinary Research Centre (CECAV), Trás-os-Montes e Alto Douro University, Quinta de Prados, Vila Real, Portugal Author-Name: F Bargallo Author-Workplace-Name: Zoològic Veterinaris, Carrer de la Conquista, Badalona, Barcelona, Spain Author-Name: J Grifols Author-Workplace-Name: Zoològic Veterinaris, Carrer de la Conquista, Badalona, Barcelona, Spain Author-Name: E Correia Author-Workplace-Name: Mathematics Departament, Trás-os-Montes e Alto Douro University, Vila Real, Portugal Author-Name: ML Pinto Author-Workplace-Name: CEMAT/IST-ID, Center for Computational and Stochastic Mathematics, Instituto Superior Técnico, University of Lisbon, Lisbon, Portugal Title: Haematological reference intervals in captive African Grey parrots (Psittacus erithacus) Abstract: The African Grey parrot (Psittacus erithacus) is one of the most popular avian pets in exotic animal practice. In this study, blood samples (n = 459) from healthy African Grey parrots (n = 239) were collected for haematological testing. Reference intervals were determined according to the guidelines of the American Society of Veterinary Clinical Pathology with the Reference Value Advisor (v2.1) freeware. The calculated reference intervals (RIs) were: packed cell volume 38-59%; haemoglobin 105-154 g/l; RBC 2.3-3.3 106/µl; MCV 145.5-199.7 fl; MHC 38.3-55.6 pg; MCHC 232-313 g/l; WBC 2.7-12.6 103/µl; heterophils 1.2-10.2 103/µl; lymphocytes 0.8-4.3 103/µl; monocytes 0.0-0.6 103/µl; basophils 0.0-0.1 103/µl; eosinophils 0.0-0.2 103/µl. To the best of our knowledge, this is the first study in psittacines to use such a high number of birds and samples to establish haematological species-specific RIs, providing a valuable tool to professionals dealing with the health of African Grey parrots. Keywords: avian medicine, blood cell count, haematology Journal: Veterinární medicína Pages: 24-31 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/15/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/15/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202101-0004.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:1:id:15-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Kim Author-Workplace-Name: Department of Veterinary Medical Imaging, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: J Ko Author-Workplace-Name: Department of Veterinary Medical Imaging, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: H Yoon Author-Workplace-Name: Department of Veterinary Medical Imaging, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Workplace-Name: Department of Veterinary Medical Imaging, College of Veterinary Medicine, Jeonbuk National University, Iksan-si, Jeollabuk-do, Republic of Korea Author-Name: H Kim Author-Workplace-Name: Helix Animal Medical Center, Seocho-gu, Seoul, Republic of Korea Author-Name: J Hwang Author-Workplace-Name: Helix Animal Medical Center, Seocho-gu, Seoul, Republic of Korea Author-Name: K Eom Author-Workplace-Name: Department of Veterinary Medical Imaging, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: J Kim Author-Workplace-Name: Department of Veterinary Medical Imaging, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Title: Clinical and imaging findings of walled-off pancreatic necrosis misdiagnosed as an intra-abdominal neoplasia in a Schnauzer dog: A case report Abstract: A 10-year-old Schnauzer presented with a 1-month history of vomiting, anorexia, and abdominal pain, and a recently detected intra-abdominal mass. The round, soft-tissue opacity masses identified on the radiography in the left mid-abdomen were confirmed as multifocal, cystic masses via ultrasonography. The necrotic masses mimicked an intra-abdominal neoplasia on the initial imaging examinations. The computed tomography (CT) clearly showed encapsulated masses with a necrotic fluid arising from the left limb of the pancreas and extending to the peripancreatic, paracolic, and perigastric regions. Based on the multimodal imaging, surgical exploration, and histopathology, the mass was diagnosed as a walled-off pancreatic necrosis (WOPN). CT is an effective diagnostic modality for diagnosing acute pancreatitis in WOPN. Keywords: acute pancreatitis, computed tomography, necrotic debris, necrotizing pancreatitis, peripancreatic fluid collections Journal: Veterinární medicína Pages: 32-39 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/123/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/123/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202101-0005.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:1:id:123-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Colakoglu HE Author-Workplace-Name: Small Animal Hospital, Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Author-Name: MO Yazlik Author-Workplace-Name: Small Animal Hospital, Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Author-Name: A Esen Author-Workplace-Name: Small Animal Hospital, Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Author-Name: AS Tunc Author-Workplace-Name: Department of Pathology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Title: Partial uterine prolapse and ovarian cysts in two Djungarian hamsters Abstract: A 2-year-old multiparous (Case 1) and a 2.5-year-old nulliparous (Case 2) Djungarian hamster each presented with a history of a prolapsed mass from the vulva. A partial uterine prolapse was diagnosed in both cases, according to the clinical and diagnostic examinations. The prolapsed mass was replaced in each hamster, and an ovariohysterectomy was performed. The histopathological examination of the removed tissues revealed a cyst and papillary hyperplasia in the ovary. This first case report, to our knowledge, demonstrates the possibility of a uterine prolapse with a cyst and papillary hyperplasia in the ovary and how to surgically manage this condition. The report could also contribute to having a better understanding of the occurrence of a uterine prolapse without parturition in hamsters. Keywords: cysts, ovariohysterectomy, prolapse, uterus Journal: Veterinární medicína Pages: 40-44 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/144/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/144/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202101-0006.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:1:id:144-2020-VETMED