Template-Type: ReDIF-Article 1.0 Author-Name: A Marchegiani Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (Macerata), Italy Author-Name: M Bazzano Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (Macerata), Italy Author-Name: MP Cassarani Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (Macerata), Italy Author-Name: R Arcelli Author-Workplace-Name: Department of Veterinary Medicine, University of Perugia, Perugia, Italy Author-Name: C Orzalesi Author-Workplace-Name: Department of Veterinary Medicine, University of Perugia, Perugia, Italy Author-Name: G Lombardo Author-Workplace-Name: CNR-IPCF, Istituto per i Processi Chimico-Fisici, Messina, Italy Author-Workplace-Name: Vision Engineering Italy srl, Rome, Italy Author-Name: M Lombardo Author-Workplace-Name: Vision Engineering Italy srl, Rome, Italy Author-Workplace-Name: Studio Italiano di Oftalmologia, Rome, Italy Author-Name: A Spaterna Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (Macerata), Italy Author-Name: R Gialletti Author-Workplace-Name: Department of Veterinary Medicine, University of Perugia, Perugia, Italy Title: Efficacy of riboflavin/UV-A corneal phototherapy as stand-alone treatment for ulcerative keratitis in horses Abstract: The purpose of this study was to assess the efficacy of riboflavin/UV-A corneal phototherapy in comparison with topical antimicrobials for the treatment of corneal ulcers in horses. Nineteen horses with a diagnosis of corneal ulceration were randomised to receive either corneal phototherapy (11 horses) or topical antimicrobial therapy (8 horses). The corneal phototherapy consisted in the application of a riboflavin-enriched ophthalmic solution onto the cornea for 20 min followed by cornea UV-A irradiation at 30 mW/cm2 for 3 min using a point-of-care UV-A device. A complete ophthalmological examination was performed at enrolment and then at day 1, 3, 7, 14, 21, 28, 35, 42, and 49 after treatment. The corneal ulcer was considered healed when the fluorescein staining was negative. The corneal phototherapy succeeded in the complete corneal wound healing in 91% cases (10/11 horses) by 12.7 ± 5.5 days. In the topical antimicrobial group, no horse showed improvement of the corneal ulceration (0/8; 0%) and two eyes had a corneal perforation. Six horses, due to an enlarged corneal lesion, underwent corneal phototherapy at day 14 and achieved complete wound healing by 17.2 ± 5.5 days in 83% cases (5/6). The corneal phototherapy was shown to be an effective and safe treatment for equine corneal ulcers. Keywords: corneal collagen cross-linking, corneal melting, equine, photobiomodulation Journal: Veterinární medicína Pages: 321-329 Volume: 66 Issue: 8 Year: 2021 DOI: 10.17221/60/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/60/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202108-0001.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:8:id:60-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H Gaspar Author-Workplace-Name: Animal and Veterinary Research Centre (CECAV), Trás-os-Montes e Alto Douro University, Quinta de Prados, Vila Real, Portugal Author-Name: F Bargallo Author-Workplace-Name: Zoològic Veterinaris, Badalona, Barcelona, Spain Author-Name: J Grifols Author-Workplace-Name: Zoològic Veterinaris, Badalona, Barcelona, Spain Author-Name: E Correia Author-Workplace-Name: Mathematics Departament, Trás-os-Montes e Alto Douro University, Quinta de Prados, Vila Real, Portugal Author-Name: ML Pinto Author-Workplace-Name: CEMAT/IST-ID, Center for Computational and Stochastic Mathematics, Instituto Superior Técnico, University of Lisbon, Lisbon, Portugal Title: Age and sex-related differences in the haematological parameters of captive African grey parrots (Psittacus erithacus) Abstract: African grey parrots (Psittacus erithacus) are very popular pets, commonly seen in avian clinical practice. Haematological profiles are critical to the understanding of several disease processes, being particularly useful as diagnostic tools in clinical practice, since birds tend to hide clinical signs of disease. We have previously proposed new haematological reference intervals (RI) for captive African grey parrots, and in the present work the basic data obtained was studied in detail to investigate the influence of factors, such as age and sex, on the haematological profile of this bird species. During an 8-year period (March 2009 to July 2017), animals (n = 239) examined in first consultations or check-ups at the Zoològic Veterinaris (Barcelona) were submitted to blood collection at different time points, rendering a total of 459 blood samples. The haematological testing was performed according to the guidelines of the American Society of Veterinary Clinical Pathology to determine the packed cell volume (PCV), haemoglobin (Hb), mean haemoglobin concentration (MHC), mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC), total erythrocyte count (TRBC), total leukocyte count (TWBC), and differential leukogram with absolute and relative counts. All the haematological testing was performed in an in-house laboratory as previously described. Animals with 0 to 4 years of age showed higher values of PCV (P < 0.001), Hb (P = 0.023) and RBC (P = 0.018), and lower values of MCHC (P = 0.008), WBC (P = 0.012) and heterophils (P < 0.001) than older animals. There were significant differences exhibited in the monocytes (P = 0.035) between different age groups. Females presented higher PCV, Hb and RBC values (P < 0.001) compared to males. Our results suggest that the age and sex influence the haematological parameters in a significant manner in African grey parrots and should be accounted for when assessing the health status of individuals from this species. Keywords: avian medicine, blood cell count, exotic pets, physiology Journal: Veterinární medicína Pages: 330-341 Volume: 66 Issue: 8 Year: 2021 DOI: 10.17221/31/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/31/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202108-0002.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:8:id:31-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M Martiniakova Author-Workplace-Name: Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic Author-Name: J Blahova Author-Workplace-Name: Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic Author-Name: V Kovacova Author-Workplace-Name: Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic Author-Name: V Mondockova Author-Workplace-Name: Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic Author-Name: R Babosova Author-Workplace-Name: Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic Author-Name: A Kalafova Author-Workplace-Name: Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: M Capcarova Author-Workplace-Name: Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: R Omelka Author-Workplace-Name: Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic Title: Effects of bee bread, Cornelian cherries treatment on the femoral bone structure using Zucker diabetic fatty rats as an animal model Abstract: This is a pilot study dealing with the reduced femoral bone structure in Zucker diabetic fatty (ZDF) rats following a 10 weeks treatment with bee bread and Cornelian cherries. The adult ZDF rats were divided into 4 groups: the E1 group received bee bread (500 mg/kg b.w. daily), the E2 group received Cornelian cherries (500 mg/kg b.w. per day), the E3 group was simultaneously supplemented by bee bread and Cornelian cherries (500 + 500 mg/kg b.w. daily), and the C group served as an untreated diabetic control. A non-significant decrease in the blood glucose, total cholesterol, triglyceride concentrations, ALP activity and a non-significant increase in the insulin, total body weight of the ZDF rats, femoral length and weight were observed in the E1, E2, and E3 groups. The treatments had no impact on the relative volume of the cortical bone, bone mineral density, bone surface and cortical bone thickness. On the contrary, the relative volume of the trabecular bone, trabecular thickness and trabecular bone surface were significantly increased in the E1 group. The relative volume of the trabecular bone and trabecular thickness were significantly elevated in the E2 group. In the E3 group, the trabecular thickness was found to have significantly increased. Single administrations of either bee bread or Cornelian cherries had more positive effects on the trabecular bone microarchitecture in the ZDF rats than the simultaneous supplementation. Keywords: bone health, Cornus mas L., diabetes mellitus, experimental animals, honey product Journal: Veterinární medicína Pages: 342-349 Volume: 66 Issue: 8 Year: 2021 DOI: 10.17221/224/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/224/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202108-0003.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:8:id:224-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Kolarova Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic Author-Name: J Velisek Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic Author-Name: Z Svobodova Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic Title: Comparison of in vitro (fish cell line) and in vivo (fish and crustacean) acute toxicity tests in aquatic toxicology Abstract: The use of in vitro (fish cell lines) is a cost-effective, very rapid, and informative tool for toxicological assessments. Using the neutral red (NR) assay, we compared the in vitro acute toxicity (20hEC50) of twenty-six chemical substances on a rainbow trout gonad cell line (RTG-2) with their in vivo acute toxicity to Barbados Millions Poecilia reticulata (48hLC50, OECD 203) and crustacean Daphnia magna (48hEC50, OECD 202). The 20hEC50 values obtained by the NR assay were higher in nearly all the cases when compared to the 48hLC50 in P. reticulata and the 48hEC50 in D. magna, indicating that the sensitivity of the RTG-2 cell line was lower compared to P. reticulata and D. magna. A high (r = 0.89) and significant (P < 0.001) correlation was recorded between the 20hEC50 values of the RTG-2 and the 48hEC50 values of D. magna. The correlation between the 20hEC50 values of the RTG-2 and the 48hLC50 values of P. reticulata was lower (r = 0.65; P < 0.001), but also significant. The authors recommend use of the NR assay on the RTG-2 cell lines as a screening protocol to evaluate the toxicity of xenobiotics in aquatic environments to narrow the spectrum of the concentrations for the fish toxicity test. Keywords: cytotoxicity, Daphnia magna, fish cell line RTG-2, NR assay, Poecilia reticulata Journal: Veterinární medicína Pages: 350-355 Volume: 66 Issue: 8 Year: 2021 DOI: 10.17221/161/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/161/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202108-0004.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:8:id:161-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S Pinna Author-Workplace-Name: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Ozzano E. (Bologna), Italy Author-Name: C Tassani Author-Workplace-Name: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Ozzano E. (Bologna), Italy Author-Name: M Rossini Author-Workplace-Name: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Ozzano E. (Bologna), Italy Author-Name: F Lanzi Author-Workplace-Name: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Ozzano E. (Bologna), Italy Title: External fixator for the treatment of narrowed pelvic canal in a cat Abstract: The aim of this study was to report the outcome of the use of an external fixator to treat a pelvic canal stenosis in a 5-month-old female cat. The cat was referred with a history of 3 weeks of intermittent signs of constipation refractory to the medical management, occurring after surgical treatment for a bilateral sacroiliac luxation and sacral fracture. The clinical examination revealed instability of the pelvis and a radiograph showed a pelvic canal stenosis and megacolon. External fixator was the method of choice to be used in this case. The manually applied tension on an external fixator resulted in a widening of the pelvic canal. At 45 days after surgery, there were no signs of constipation, and the radiological examination showed progressive bone healing. At 18 months post-op, the cat had no abnormalities both on the clinical examination and on the radiography. In conclusion, the use of an external fixator led to the widening of the pelvic canal using a minimally invasive procedure. To the authors' knowledge, this case report represents the first surgical description and clinical outcome of the widening of the pelvic canal in cats using an external skeletal fixator. Keywords: constipation, minimally invasive procedure, sacral index, stenosis Journal: Veterinární medicína Pages: 356-362 Volume: 66 Issue: 8 Year: 2021 DOI: 10.17221/173/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/173/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202108-0005.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:8:id:173-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N Ruiz-Suarez Author-Workplace-Name: Department of Veterinary Medical Imaging and Small Animal Orthopaedics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Author-Name: SFM Bhatti Author-Workplace-Name: Small Animal Department, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Author-Name: M Hermans Author-Workplace-Name: Small Animal Department, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Author-Name: CB Silva Author-Workplace-Name: Department of Veterinary Medical Imaging and Small Animal Orthopaedics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Author-Name: M Hesta Author-Workplace-Name: Department of Veterinary Medical Imaging and Small Animal Orthopaedics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Title: Food hypersensitivity and feline hyperaesthesia syndrome (FHS): A case report Abstract: A 2-year-11-month-old female spayed cat was at the Small Animal Teaching Hospital of Ghent University presenting with hyperactivity, scratching and licking all over her body and an abnormal urination behaviour. Nothing remarkable was found on the dermatology and neurological examination. Based on the owner's history and video material, the presence of feline hyperaesthesia syndrome (FHS) was hypothesised. A symptomatic treatment with gabapentin was established for a month without any significant improvement. An elimination diet with hydrolysed protein sources was started and, as a result, the dose of gabapentin was reduced after three days and completely stopped after one week. With the exception of two non-intentional exposures to non-hypoallergenic diets and the challenge with new protein sources by the owner, the cat has been free of symptoms, with the exception of a slight reaction in the lumbar area (significantly reduced in comparison before starting the diet), and without the use of medication. In conclusion, an elimination diet should be considered as part of the diagnostic plan for FHS and should not be delegated to the last step if the patient's condition allows it. Keywords: elimination diet, hypoallergenic diet, rolling skin Journal: Veterinární medicína Pages: 363-367 Volume: 66 Issue: 8 Year: 2021 DOI: 10.17221/118/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/118/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202108-0006.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:8:id:118-2020-VETMED