Template-Type: ReDIF-Article 1.0 Author-Name: Y Jeong Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Author-Name: T Yun Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Author-Name: H Kim Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Author-Name: Y Koo Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Author-Name: JH Kang Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Author-Name: MP Yang Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Author-Name: BT Kang Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Title: Induction of atopic dermatitis by epicutaneous sensitisation with Dermatophagoides farinae in nongenetically predisposed Beagle dogs Abstract: This study was performed to induce atopic dermatitis (AD) using nongenetically predisposed Beagle dogs. Five healthy Beagle dogs were used. Twice weekly for 12 weeks, the dogs were painted on the axillae and groin with a solution of Dermatophagoides farinae (D. farinae). Each dog was thereafter placed in a cage where a house dust mite (HDM) solution was applied on the bottom of the cage. The dog remained in the cage for 3 h daily for 3 consecutive days for the environmental exposure to HDM. Serum samples were collected at 0 week and 6 weeks after sensitisation, and at 0 h and 72 h after the environmental exposure. During the environmental exposure, skin biopsies were obtained at 0 h, 36 h, and 72 hours. After the first environmental exposure, no dog had any marked clinical sign. An additional sensitisation was subsequently administered for 10-13 weeks. Three of the five dogs developed pruritic dermatitis with skin lesions after the second exposure. The histopathology of the lesions revealed severe infiltration of inflammatory cells and dermal oedema. The levels of D. farinae-specific IgE were also elevated. This study demonstrated that AD could be induced by epicutaneous sensitisation with HDM in nongenetically predisposed dogs. Keywords: animal model, atopy, D. farinae, house dust mite, IgE Journal: Veterinární medicína Pages: 413-422 Volume: 66 Issue: 10 Year: 2021 DOI: 10.17221/58/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/58/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202110-0001.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:10:id:58-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H Isidan Author-Workplace-Name: Department of Virology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, Sivas, Turkey Author-Name: T Turan Author-Workplace-Name: Department of Virology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, Sivas, Turkey Title: A comprehensive study of canine parvoviruses (Carnivore protoparvovirus 1, Carnivore bocaparvovirus 1 and 2) from shelter dogs in Turkey Abstract: A total of 150 rectal swab samples were collected from diarrheic dogs from the Sivas Municipal Animal Shelter, Turkey in April 2018. While 127 faecal samples were gathered from adults, 23 samples were collected from puppies. Canine parvovirus type 2 (Carnivore protoparvovirus 1), Canine minute virus (Carnivore bocaparvovirus 1) and Canine bocavirus (Carnivore bocaparvovirus 2) were examined by PCR with three sets of novel primers. Some of the amplicons were subjected to molecular sequencing and molecular analysis. Three faecal (2.36%) samples were found to be positive for Carnivore protoparvovirus 1, five faecal samples (3.94%) were found to be positive for Carnivore bocaparvovirus 1 and three samples (2.36%) were found to be positive for Carnivore bocaparvovirus 2 among 127 adult dogs. On the other hand, 14/23 faecal samples (60.87%) were found to be positive for Carnivore protoparvovirus 1, 8/23 faecal samples (34.78%) were found to be positive for Carnivore bocaparvovirus 1, and 6/23 faecal samples (26.09%) were found to be positive for Carnivore bocaparvovirus 2 from the puppies. Moreover, we detected two distinct clades of Carnivore bocaparvovirus 1 and 2 according to the molecular analysis. To the best of our knowledge, this is the first report for the direct detection of Carnivore bocaparvovirus 1 and 2 in Turkey. Keywords: dog parvovirus, molecular analysis, NS1 gene, phylogeny, sequencing, VP2 gene Journal: Veterinární medicína Pages: 423-430 Volume: 66 Issue: 10 Year: 2021 DOI: 10.17221/130/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/130/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202110-0002.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:10:id:130-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: TN Thi Author-Workplace-Name: Institute of Veterinary Research and Development of Central Vietnam, Vinh Hoa, Nha Trang, Khanh Hoa, Vietnam Author-Name: H Vu-Khac Author-Workplace-Name: Institute of Veterinary Research and Development of Central Vietnam, Vinh Hoa, Nha Trang, Khanh Hoa, Vietnam Author-Name: TN Duc Author-Workplace-Name: Institute of Veterinary Research and Development of Central Vietnam, Vinh Hoa, Nha Trang, Khanh Hoa, Vietnam Title: Characterisation of Clostridium perfringens isolated from chickens in Vietnam Abstract: The objective of this study was isolating and characterising Clostridium perfringens from chickens in Vietnam and identifying virulence factors involved with enteritis. Five hundred thirty-one faecal and sixty-eight intestinal samples were collected from healthy and diseased chickens for the C. perfringens isolation. The presence of virulence factors was determined by multiplex PCR. The netB gene of the selected isolates was sequenced and checked for its expression by SDS-PAGE. Two hundred seventy-two C. perfringens isolates were collected. All of them were shown to be positive for the cpa gene. The netB gene was detected in 26.56% of the C. perfringens isolates from the healthy chickens, while 43.45% of the isolates from the faeces and 45% of the isolates from the intestinal samples were positive for this gene in the diseased birds. All eight isolates positive to netB from the diseased chickens showed 100% identity in the netB sequence and produced the NetB toxin in vitro, whereas only two out of eight healthy chicken-derived isolates produced this toxin. Nine out of ten chickens experimentally infected with the C. perfringens netB-positive isolate showed typical signs of enteritis. The cpa gene was the most prevalent virulence factor identified in the bacteria C. perfringens, but the netB gene could be a major player responsible for necrotic enteritis progression in chickens. Keywords: cpa, necrosis enteritis, netB, poultry, virulence factor Journal: Veterinární medicína Pages: 431-439 Volume: 66 Issue: 10 Year: 2021 DOI: 10.17221/209/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/209/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202110-0003.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:10:id:209-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O Karabulut Author-Workplace-Name: Department of Biometrics, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Turkey Title: Estimation of the external quality characteristics of goose eggs of known breadth and length Abstract: In this study, we investigated whether the mathematical formulas, which generally reveal the external quality characteristics of living organisms, yield the correct results in goose eggs. For this purpose, three genotypes and 555 eggs were studied that were grouped into Native (n = 356), Chinese (n = 163) and Linda (n = 36), which were raised in Aksaray province. The averages in the Native, Chinese and Linda geese were 5.43, 5.38 and 5.7 cm, respectively, in breadth; were 8.12, 8.03 and 8.67 cm in length, respectively; the shape index was 66.9, 67.2 and 66.7 cm, respectively; the egg volume was 142.8, 138.8 and 172.5 cm3, respectively; the egg surface area was 136.9, 134.3 and 155.4 cm2, respectively; the egg weight was 159.1, 154.5 and 192.3 g, respectively; the specific gravity of the eggs was 1.11, 1.11 and 1.12 g/cm3, respectively; the shell thickness was 0.510, 0.504 and 0.555 mm, respectively; the shell weight was 14.79, 14.32 and 18.26 g, respectively; the shell volume was 70.06, 67.82 and 86.41 cm3, respectively; the shell specific gravity was 2.111 3, 2.111 0 and 2.113 5 g/cm3, respectively; and the shell ratio was 9.29, 9.26 and 9.49 g, respectively. The number of pores is calculated as 14 828, 14 502 and 17 152, respectively. Although the formulas used give compatible results, there is a need for formulas that calculate closer to the truth rather than formulas that calculate the egg weight and egg shell weight. Keywords: egg measurements, equations, geese, shape index Journal: Veterinární medicína Pages: 440-447 Volume: 66 Issue: 10 Year: 2021 DOI: 10.17221/187/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/187/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202110-0004.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:10:id:187-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L Pennasilico Author-Workplace-Name: School of Bioscience and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: C Di Bella Author-Workplace-Name: School of Bioscience and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: R Botto Author-Workplace-Name: School of Bioscience and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: E Murgia Author-Workplace-Name: School of Bioscience and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: V Riccio Author-Workplace-Name: School of Bioscience and Veterinary Medicine, University of Camerino, Matelica, Italy Author-Name: AP Piccionello Author-Workplace-Name: School of Bioscience and Veterinary Medicine, University of Camerino, Matelica, Italy Title: Use of micro-grafts in a chronic infected open wound after limb amputation in a cat Abstract: In human medicine, skin grafting is an innovative surgical technique widely used in reconstructive surgery to repair skin loss. This case evaluated the effectiveness of a treatment with dermal micro-grafting obtained through the Rigenera® technology in a chronic open wound resulting from a suture dehiscence of a limb amputation in a cat. Significant differences were observed between the aesthetic aspects of the injury using traditional treatments (cleaning and curettage) and the regenerative technology. The results showed that the healing periods were significantly reduced after the Rigenera® treatment and that, moreover, a perfect skin status and a complete reduction in the wound area (100%) were achieved in one month. Given these results, Rigenera® has proven to be a simple yet highly effective method in the treatment of inactivated chronic wounds. Keywords: chronic wound, feline traumatology, regenerative medicine, Rigenera®, system, skin injury Journal: Veterinární medicína Pages: 448-455 Volume: 66 Issue: 10 Year: 2021 DOI: 10.17221/197/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/197/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202110-0005.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:10:id:197-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: PA Socha Author-Workplace-Name: Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: BM Socha Author-Workplace-Name: Department of Genetics and Animal Breeding, Faculty of Veterinary Medicine and Animal Science, Poznan University of Life Sciences, Poznan, Poland Title: Congenital hydrocephalus in a Maine Coon foetus detected using ultrasound during pregnancy Abstract: A congenital defect that blocks the cerebrospinal fluid outflow is the most common cause of hydrocephalus in young cats. It usually manifests itself within a few weeks of birth. Diagnostic imaging is essential. Therefore, it was decided to report on, for the first time, a clinical case of congenital hydrocephalus in a Maine Coon foetus detected using ultrasound during pregnancy. A difference between the size of the skull bones in the Maine Coon foetuses was recognised during a routine pregnancy ultrasound. Analysis of the sonogram of the bones and brain structures of the diagnosed foetus revealed that the features were highly suggestive of a hydrocephalus. Five clinically healthy kittens and one with hydrocephalus were delivered without complications. The necropsy of the malformed foetus was consistent with the ultrasonographic findings. Bacteriological and virological tests were performed and all were negative which suggest a congenital background of the malformation. This is the first reported case of an intrauterine ultrasound diagnosis of congenital hydrocephalus in a Maine Coon of multiple gestation. The ultrasound control along with the foetometry seem to be useful tools to recognise certain pathologies of pregnancy in cats, including congenital malformations of various origins, which is of significant diagnostic and prognostic importance. Keywords: cat, foetal malformation, foetometry, gestation, ultrasonography Journal: Veterinární medicína Pages: 456-460 Volume: 66 Issue: 10 Year: 2021 DOI: 10.17221/204/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/204/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202110-0006.txt Handle: RePEc:caa:jnlvet:v:66:y:2021:i:10:id:204-2020-VETMED