Template-Type: ReDIF-Article 1.0 Author-Name: F Rezazadeh Author-Workplace-Name: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Author-Name: N Pourebrahimi Author-Workplace-Name: DVM, University of Tabriz, Tabriz, Iran Author-Name: R Ghotaslou Author-Workplace-Name: Research Centre of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran Author-Name: MG Nasab Author-Workplace-Name: DVM, University of Tabriz, Tabriz, Iran Author-Name: MY Memar Author-Workplace-Name: Research Centre of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran Title: Frequency of endoscopic findings of equine squamous gastric disease (ESGD) in rural horses and its association with Helicobacter pylori virulence genes Abstract: Equine gastric ulcer syndrome (EGUS) is a multifactorial disorder and one of the most common diseases in horses. The objective of this research was to detect one of the potential risk factors of equine squamous gastric disease (ESGD), the Helicobacter pylori specific gene, and tracing the presence of the duodenal ulcer-promoting gene (dupA) as a possible virulence marker. Gastric fluid together with faecal samples were collected from twenty rural horses from around Tabriz, Iran. Throughout the endoscopic examinations, the type, numbers, severity, and the location of the lesions were documented. Nine of twenty horses exhibited macroscopic lesions in the squamous mucosa that were later classified into grades 1, 2, 3, and 4. Only three of these horses exhibited H. pylori in their gastric fluid samples, whereas all faecal samples were H. pylori-negative. All the H. pylori-positive cases manifested severe forms of ESGD (grades 3-4). The age and sex were both unrelated to the lesion severity and ESGD status in this study. Research is required to further discuss the virulence aspects of dupA regarding ESGD. Keywords: dupA, EGUS, H. pylori Journal: Veterinární medicína Pages: 61-69 Volume: 67 Issue: 2 Year: 2022 DOI: 10.17221/154/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/154/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202202-0001.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:2:id:154-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: BW Minto Author-Workplace-Name: Department of Veterinary Clinic and Surgery, Faculty of Agrarian and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil Author-Name: LMI Diogo Author-Workplace-Name: Department of Veterinary Clinic and Surgery, Faculty of Agrarian and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil Author-Name: CR de Andrade Author-Workplace-Name: Department of Veterinary Clinic and Surgery, Faculty of Agrarian and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil Author-Name: WS Santos Junior Author-Workplace-Name: Veterinária Vila Isabel, Rio de Janeiro, Rio de Janeiro, Brazil Author-Name: LG de Faria Author-Workplace-Name: Department of Veterinary Clinic and Surgery, Faculty of Agrarian and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil Author-Name: FYK Kawamoto Author-Workplace-Name: Department of Veterinary Clinic and Surgery, Faculty of Agrarian and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil Author-Name: TC Prada Author-Workplace-Name: Department of Veterinary Clinic and Surgery, Faculty of Agrarian and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil Author-Name: LGGG Dias Author-Workplace-Name: Department of Veterinary Clinic and Surgery, Faculty of Agrarian and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil Title: Clinical and radiographic evaluation of subsidence in two femoral stem models for a total hip replacement in dogs Abstract: Canine cementless total hip arthroplasty (THA) is a successful technique for the management of hip arthrosis; however, serious potential complications, such as femoral fractures and subsidence of the femoral stem, can occur. To evaluate the effect of two femoral stem designs in reducing subsidence in dogs, twenty-four hips from twenty-one dogs undergoing THA were assessed. The twenty-four arthroplasties were divided into two experimental groups: G1 with a first generation, and G2, which is the second generation, system were used. All the dogs were clinically and radiographically evaluated immediately post-operatively and at 30 (M1) and 120 days post-operatively (M2). Three of ten arthroplasties in the G1 system had subsidence and six of fourteen had subsidence in the G2 system. Both systems are effective for management of hip arthrosis. The varus positions with the sub-optimal filling were not related to the subsidence. The two stem designs both showed some resistance to subsidence, but clinical problems were not identified in any case. Keywords: arthroplasty, complications, dysplasia, press-fit Journal: Veterinární medicína Pages: 70-77 Volume: 67 Issue: 2 Year: 2022 DOI: 10.17221/36/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/36/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202202-0002.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:2:id:36-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M Habova Author-Workplace-Name: Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic Author-Name: M Pyszko Author-Workplace-Name: Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic Author-Name: O Horak Author-Workplace-Name: Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic Author-Name: E Cermakova Author-Workplace-Name: Avian and Exotic Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic Author-Name: V Paral Author-Workplace-Name: Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic Title: Differences in the anatomy of the lower respiratory tract in selected species of the order Testudines Abstract: The lower airways of turtles consist of the larynx, trachea and bronchi. Due to incomplete information about the anatomical structure of the lower respiratory tract in turtles, we decided to explore this topic more deeply. The material for this study included 95 turtle cadavers. Terrestrial tortoises were represented by 63 individuals from 8 species, aquatic and semi-aquatic turtles by 32 individuals from 11 species. The sex ratio was 54 females to 41 males. In addition to the anatomical autopsies and assessments of the macroscopic structures, numerous measurements were performed. The length, width and height of the larynx and trachea, and the length of the bronchi were measured. We found clinical significance in two parameters. The first one is the formation of the rima glottidis, which is significantly shorter and narrower in the aquatic turtles than in the terrestrial tortoises. It follows that for these species, we should use a smaller diameter of endotracheal cannula for intubation. The second parameter is a very short trachea in tortoises of the genus Testudo. The length of the trachea is only a few centimetres, which significantly increases the risk of intubation into one bronchus only. Keywords: intubation of turtle and tortoise, length of trachea, rima glottidis, tracheal bifurcation Journal: Veterinární medicína Pages: 78-86 Volume: 67 Issue: 2 Year: 2022 DOI: 10.17221/64/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/64/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202202-0003.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:2:id:64-2021-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Yang Author-Workplace-Name: Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China Author-Name: P Zhuang Author-Workplace-Name: Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China Author-Name: Z Cheng Author-Workplace-Name: Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China Author-Name: G Wang Author-Workplace-Name: Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China Title: Comparative analysis of the exosomal contents of DF-1 cells infected by ALV-J Abstract: Exploration of the abnormal expression of exosomal molecules during the infection of avian leukosis virus subgroup J (ALV-J) is essential to provide a deeper understanding of the exosome's role in the viral pathogenesis involved. The study aimed to investigate the differentially expressed proteins and miRNAs of the exosomes derived from DF-1 cells infected by ALV-J, their gene function and involved signal pathways. We isolated exosomes from DF-1 cells infected by ALV-J. The differentially expressed proteins and miRNAs of the exosomes were determined by proteomics and transcription detection technology. A Gene Ontology (GO) analysis and a Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway analysis identified the miRNAs target genes and the signal pathways regulated by the different proteins or/and miRNAs. A total of 116 proteins (58 upregulated and 58 downregulated) and 3 miRNAs (all upregulated) were determined. These proteins were involved in 155 signal pathways, in which the highest number of proteins involved in the cancer pathway was (up to) seven. The target genes of the miRNAs were involved in 3 signal pathways. Both the proteins and target genes of the miRNAs were involved in the Ribosome pathway and ECM-receptor interaction pathway. The results suggested that the ALV-J infection changed the proteins and miRNAs of the exosomes significantly. Keywords: avian leukosis virus subgroup J (ALV-J), exosomes, proteomics, transcriptomics Journal: Veterinární medicína Pages: 87-98 Volume: 67 Issue: 2 Year: 2022 DOI: 10.17221/141/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/141/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202202-0004.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:2:id:141-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P Makovicky Author-Workplace-Name: Infectious Diseases and Preventive Medicine, Veterinary Research Institute, v. v. i., Brno, Czech Republic Author-Name: AV Makarevich Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production, Lužianky, Slovak Republic Author-Name: P Makovicky Author-Workplace-Name: Department of Biology, Faculty of Education, J. Selye University, Komárno, Slovak Republic Author-Name: A Seidavi Author-Workplace-Name: Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran Author-Name: L Vannucci Author-Workplace-Name: Laboratory of Immunotherapy, Institute of Microbiology of the Czech Academy of Sciences, v. v. i., Prague, Czech Republic Author-Name: K Rimarova Author-Workplace-Name: Department of Public Health and Hygiene, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak republic Title: Benign ovarian teratoma in the dog with predominantly nervous tissue: A case report Abstract: Ovarian teratomas are rare neoplasms in female dogs, and they are characterised by the proliferation of tissues of embryonic origin. Most teratomas are benign, but a histological diagnosis is important for clinicians. The objective of this article is to describe a benign ovarian teratoma in a dog, which was found on the street and was appearing like pregnant. A veterinary inspection by palpation documented an enlarged abdomen with a mass of tough matter located on the right side in the abdominal-pelvic part. An ultrasound examination presumed neoplastic mass in region of ovary. A bilateral ovariohysterectomy was performed and the subsequent histological evaluation revealed a benign ovarian teratoma with a histochemically and immunohistochemically verified nervous tissue. After one year, no distant metastases were found and the dog was recognised as being clinically healthy without problems. On the basis of the ultrasonography diagnostics and histopathological analyses, we have demonstrated the occurrence of a benign ovarian teratoma in a dog. Keywords: germ cell neoplasm, ovary, veterinary pathology Journal: Veterinární medicína Pages: 99-104 Volume: 67 Issue: 2 Year: 2022 DOI: 10.17221/55/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/55/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202202-0005.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:2:id:55-2021-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Kim Author-Workplace-Name: Veterinary Emergency Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: D Kim Author-Workplace-Name: Veterinary Emergency Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: D Seo Author-Workplace-Name: Veterinary Emergency Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: H Hwang Author-Workplace-Name: Veterinary Emergency Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: Y Kim Author-Workplace-Name: Veterinary Emergency Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: T Chung Author-Workplace-Name: Veterinary Emergency Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: S Lim Author-Workplace-Name: Veterinary Emergency Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: H Lee Author-Workplace-Name: Veterinary Emergency Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: M Kim Author-Workplace-Name: Veterinary Emergency Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: S Park Author-Workplace-Name: Veterinary Internal Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: H Youn Author-Workplace-Name: Veterinary Internal Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Title: Clinical application of both amniotic membranes and adipose derived mesenchymal stem cells in a cat with a large skin defect Abstract: Surgical procedures on large skin defects can be challenging in the short term due to the size of the lesion, infection, and tissue defect. A regenerative therapy for skin wounds has been applied to promote the healing process. An 8-month-old, Korean domestic short-haired female cat, weighing 3 kg, was rescued with extensive defects on the right flank to right inguinal region caused by bite wounds. In this case, amniotic membranes and adipose-derived mesenchymal stem cells were used as the regenerative therapy to treat the large skin defect rather than a surgical intervention alone. To the best of our knowledge, this is the first report of a case with of a large skin defect treated by applying allogeneic amniotic membranes and allogeneic mesenchymal stem cells to a cat. Keywords: feline, healing process, regenerative therapy, skin wound Journal: Veterinární medicína Pages: 105-111 Volume: 67 Issue: 2 Year: 2022 DOI: 10.17221/155/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/155/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202202-0006.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:2:id:155-2020-VETMED