Template-Type: ReDIF-Article 1.0 Author-Name: M Candela Andrade Author-Workplace-Name: Department of Human Anatomy, Health and Medical University Potsdam, Potsdam, Brandenburg, Germany Author-Name: P Slunsky Author-Workplace-Name: Surgical Department, Small Animal Hospital, Anicura Kleintierspezialisten Augsburg, Augsburg, Bavaria, Germany Author-Name: LG Klass Author-Workplace-Name: Department of Veterinary Medicine, Institute for Parasitology and Tropical Veterinary Medicine, Freie Universitaet Berlin, Berlin, Germany Author-Name: L Brunnberg Author-Workplace-Name: Department of Veterinary Medicine, Small Animal Clinic, Freie Universitaet Berlin, Berlin, Germany Title: Patellar luxation and concomitant cranial cruciate ligament rupture in dogs - A review Abstract: A patellar luxation and concomitant cranial cruciate ligament rupture is a common pathology in dogs. Diagnosis is based on clinical evidence of a patellar luxation and stifle joint instability. However, diagnostic imaging is required to assess the number of skeletal deformities and signs of instability. Surgical options include both soft tissue and osseous techniques, although, in most cases, a combination of multiple procedures is necessary to correct the patellar luxation and restore the stifle joint stability. Complication rates are generally low, but can include reluxation and implant-associated complications. This article describes the patellar luxation and cranial cruciate ligament rupture signs in dogs, including the clinical presentation and diagnosis, and discusses current treatment options. Keywords: canine lameness, knee surgery, stifle pathology Journal: Veterinární medicína Pages: 163-178 Volume: 67 Issue: 4 Year: 2022 DOI: 10.17221/111/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/111/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202204-0001.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:4:id:111-2021-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Kaabi Author-Workplace-Name: Zoology Department, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia Author-Name: I Barakat Author-Workplace-Name: Cell Biology Department, National Research Centre, Dokki, Giza, Egypt Author-Name: R Alajmi Author-Workplace-Name: Zoology Department, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia Author-Name: A Al-Khalaf Author-Workplace-Name: Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia Author-Name: O Mehdi Author-Workplace-Name: Department of Bioinformatics, College of Life Sciences, Shanxi Agricultural University, Taigu, P.R. China Title: Ameliorative effect of bee products on in vitro maturation of sheep oocytes Abstract: The present study was undertaken to investigate the possible stimulating effects of Nigella sativa (N. sativa) honey, natural Saudi Sider honey, and honeybee pollen to the in vitro maturation (IVM) medium of sheep oocytes on their subsequent development. Hence, immature oocytes were exposed to various concentrations of natural Nigella sativa (N. sativa), and Saudi Sider honey (5, 10, and 20%), as well as honeybee pollen (1, 10, 50 μg/ml) during an in vitro maturation period (24 hours). After the exposure time, the maturation rate, glutathione (GSH) concentration, and candidate gene expression (GDF-9, MPF, CMOS, IGF-1, and BAX) were evaluated. Our results showed that the maturation rate was higher in the groups challenged with the lowest level of the bee products (5% and 1 μg/ml) when compared with that in the control group; where the mean number of oocytes in the metaphase II stage reached 0.360 for the honeybee pollen-treated group, 0.293 for the N. sativa-treated group, and 0.203 for the natural Saudi Sider honey-treated group. The glutathione level was significantly increased in the group exposed to N. sativa honey when compared with the other groups. Concerning the gene expression results, the Saudi Sider honey treatment showed the best results for all the genes except the CMOS gene, which was significantly higher than the GI and GII groups and lower than the GIV group and the BAX gene which did not show a significant difference when compared with the other groups. In conclusion, the addition of natural honey and honeybee pollen at a low concentration to an IVM medium improved the in vitro maturation rate, increased the glutathione level, and gene expression of the in vitro matured ovine oocytes. Keywords: honeybee pollen, gene expression, glutathione, Nigella sativa, nuclear maturation, Sider honey Journal: Veterinární medicína Pages: 179-189 Volume: 67 Issue: 4 Year: 2022 DOI: 10.17221/211/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/211/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202204-0002.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:4:id:211-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Marchegiani Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (Macerata), Italy Author-Name: R Gialletti Author-Workplace-Name: Department of Veterinary Medicine, University of Perugia, Perugia, Italy Author-Name: MP Cassarani Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (Macerata), Italy Author-Name: M Cerquetella Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (Macerata), Italy Author-Name: AR Attili Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (Macerata), Italy Author-Name: G Lombardo Author-Workplace-Name: CNR-IPCF, Istituto per i Processi Chimico-Fisici, Messina, Italy Author-Workplace-Name: Vision Engineering Italy srl, Rome, Italy Author-Name: M Lombardo Author-Workplace-Name: Vision Engineering Italy srl, Rome, Italy Author-Workplace-Name: Studio Italiano di Oftalmologia, Rome, Italy Author-Name: A Spaterna Author-Workplace-Name: School of Biosciences and Veterinary Medicine, University of Camerino, Matelica (Macerata), Italy Author-Name: R Arcelli Author-Workplace-Name: Department of Veterinary Medicine, University of Perugia, Perugia, Italy Title: Riboflavin/UV-A corneal phototherapy as stand-alone management of ulcerative keratitis in dogs Abstract: Corneal ulcers are one of the most common ocular disorders in veterinary ophthalmology and several factors can negatively influence the efficacy of the currently available therapeutic options, leading to a loss of corneal transparency and, thus, vision. Twenty-five dogs with clinical signs of corneal ulcers were randomised to receive either corneal phototherapy (16 dogs; study group) or topical standard medical therapy (9 dogs; control group). The riboflavin/UV-A corneal phototherapy (PACK-CXL) consisted in the application of a riboflavin ophthalmic solution (Visioflavin®; Vision Engineering Italy srl, Rome, Italy) onto the cornea for 20 min followed by 30 mW/cm2 UV-A irradiance for 3 min using a point-of-care UV-A device (Vetuvir®; Vision Engineering Italy srl, Rome, Italy). The complete healing of the ulcerative lesion was defined as the complete restoration of the corneal epithelial integrity with negative fluorescein staining. The corneal phototherapy achieved complete corneal healing in all the dogs by 20.5 ± 7.8 days. In the control group, only two dogs achieved complete healing by 21.5 ± 15.6 days. This intervention may represent a valid option to hasten corneal wound healing and a clinical resolution of ulcerative keratitis in dogs. Keywords: corneal melting, corneal ulcer, cross-linking, keratitis, riboflavin, UV-A Journal: Veterinární medicína Pages: 190-198 Volume: 67 Issue: 4 Year: 2022 DOI: 10.17221/46/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/46/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202204-0003.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:4:id:46-2021-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: T Souza Author-Workplace-Name: Veterinary Teaching Hospital, Federal University of Bahia, Salvador, Bahia State, Brazil Author-Name: N Anjos Author-Workplace-Name: Veterinary Teaching Hospital, Federal University of Bahia, Salvador, Bahia State, Brazil Author-Name: I Kersul Author-Workplace-Name: Veterinary Teaching Hospital, Federal University of Bahia, Salvador, Bahia State, Brazil Author-Name: E Martins Filho Author-Workplace-Name: Veterinary Teaching Hospital, Federal University of Bahia, Salvador, Bahia State, Brazil Author-Name: T Nunes Author-Workplace-Name: Department of Anatomy, Pathology and Veterinary Clinics, School of Veterinary Medicine and Zootechnics, Federal University of Bahia, Salvador, Bahia State, Brazil †In memoriam Author-Name: V Barbosa Author-Workplace-Name: Department of Anatomy, Pathology and Veterinary Clinics, School of Veterinary Medicine and Zootechnics, Federal University of Bahia, Salvador, Bahia State, Brazil †In memoriam Title: Effects of dexmedetomidine or tramadol continuous rate infusions on the propofol requirements and cardiorespiratory variables in propofol-ketamine-midazolam anaesthetised cats Abstract: The aim of this study was to compare the effects of midazolam-ketamine alone or in combination with dexmedetomidine or tramadol in a constant rate infusion (CRI) on the minimum infusion rate (MIR) of propofol and the cardiorespiratory function in cats undergoing an ovariohysterectomy (OH). This was a prospective, randomised, blinded clinical study. Twenty-four healthy female mixed-breed cats were premedicated with ketamine and midazolam. Propofol was used for the induction and maintenance of the anaesthesia (starting at 18 mg/kg/h). Cats were assigned to groups (n = 8) to receive one of the following intravenous treatments: midazolam-ketamine group (MKG; ketamine 0.6 mg/kg/h); midazolam-ketamine-dexmedetomidine group [MKDG; ketamine 0.6 mg/kg/h and dexmedetomidine loading dose (LD) 1 mg/kg, CRI 1 mg/kg/h)] or midazolam-ketamine-tramadol group [MKTG; ketamine 0.6 mg/kg/h and tramadol (LD 2 mg/kg, CRI 1 mg/kg/h)]. During the OH, the propofol infusion was adjusted based on the clinical signs to maintain adequate anaesthetic depth. Selected variables were measured before (T0) and after (T1) the anaesthesia induction and during six surgical time points (T2-T7). The mean arterial pressure was higher and the heart rate was lower in MKDG at T1 (than in MKG and MKTG). The mean ± SD MIR of propofol were 17.4 ± 3.2, 15.0 ± 2.8 and 12.6 ± 3.5 mg/kg/h for MKG, MKTG, and MKDG, respectively. We conclude that, compared to midazolam-ketamine alone, midazolam-ketamine-tramadol and midazolam-ketamine-dexmedetomidine reduced the MRI of propofol by 13.8% and 27.5%, respectively, without significant changes in the selected indicators. Keywords: alpha-2 agonist, anaesthetic-sparing effect, feline, total intravenous anaesthesia Journal: Veterinární medicína Pages: 199-205 Volume: 67 Issue: 4 Year: 2022 DOI: 10.17221/138/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/138/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202204-0004.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:4:id:138-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: JH An Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: KB Kim Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: SC Kwon Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: HJ Kim Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: MO Ryu Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: YI Oh Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Author-Name: JO Ahn Author-Workplace-Name: Department of Veterinary Internal Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National University, Chuncheon-si, Republic of Korea Ju-Hyun An and Kyeong-Bo Kim contributed equally to this work Author-Name: HY Youn Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea Title: Canine adipose tissue-derived mesenchymal stem cell therapy in a dog with renal Fanconi syndrome Abstract: Renal Fanconi syndrome (RFS) affects the proximal tubular resorption in the nephrons. This causes excessive loss of key solutes through the urine. In a canine patient, we successfully managed the renal tubular acidosis and proteinuria caused by RFS via transplantation of canine adipose tissue-derived mesenchymal stem cells (cAT-MSCs). cAT-MSCs were administered ten times at intervals of 2-4 weeks. The post-therapy check-up revealed that the cAT-MSC treatment improved the renal tubular acidosis and proteinuria. Hence, a cAT-MSC transplant may be considered as an adjuvant therapy in veterinary medicine to initiate and maintain relief of RFS-induced acidosis and proteinuria. Keywords: glucosuria, proteinuria, renal tubular acidosis Journal: Veterinární medicína Pages: 206-211 Volume: 67 Issue: 4 Year: 2022 DOI: 10.17221/213/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/213/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202204-0005.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:4:id:213-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: T Yoshida Author-Workplace-Name: Department of Veterinary Surgery, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan Author-Name: C Chieh-Jen Author-Workplace-Name: Department of Veterinary Surgery, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, Nihon University, Fujisawa-shi, Japan Author-Name: ASa Mandour Author-Workplace-Name: Department of Veterinary Surgery, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan Author-Workplace-Name: Department of Animal Medicine, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt Author-Name: HAMM Hendawy Author-Workplace-Name: Department of Veterinary Surgery, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan Author-Name: N Machida Author-Workplace-Name: Department of Veterinary Clinical Oncology, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan Author-Name: A Uemura Author-Workplace-Name: Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-shi, Japan Author-Name: R Tanaka Author-Workplace-Name: Department of Veterinary Surgery, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan Title: Clinical and necropsy evaluation of endocardial fibroelastosis in a mixed-breed cat with left side heart failure Abstract: A two-month-old, male intact, mixed-breed cat weighing 0.6 kg was presented with respiratory distress and anorexia. From the transthoracic echocardiographic, reduced fractional shortening (FS) and increased endocardial echogenicity were recognised with severe congestive heart failure (CHF). The kitten was administered an antibiotic and pimobendane under oxygen supplementation in an ICU cage. However, the respiratory condition worsened and the cat died the next day, and the subsequent necropsy and histopathology examinations confirmed endocardial fibroelastosis (EFE). There is a lack of information regarding the antemortem cardiac function evaluated by tissue Doppler imaging (TDI) in EFE cases. We report on the echocardiographic findings including the TDI in the EFE cat with a concomitant necropsy and histopathology confirmation in this paper. The echocardiographic findings showed presence of a ventricular false tendon within the left ventricle, a decrease in the left ventricular contractility (FS 11.1%, and a marked CHF). In this case, the echocardiographic findings were consistent with the human counterpart. However, these findings were like those of dilated cardiomyopathy and, hence, non-specific to EFE. As a result, veterinarians should keep in mind that endocardial fibroelastosis might be a possible reason for respiratory distress resulting from CHF with a low fractional shortening in young cats. Keywords: echocardiography, endocardial fibroelastosis, kitten, tissue Doppler imaging Journal: Veterinární medicína Pages: 212-217 Volume: 67 Issue: 4 Year: 2022 DOI: 10.17221/52/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/52/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202204-0006.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:4:id:52-2021-VETMED