Template-Type: ReDIF-Article 1.0 Author-Name: M Demirpence Author-Workplace-Name: Bacteriological Diagnosis Laboratory, Directorate of Veterinary Department, Nicosia, North Cyprus Author-Name: AM Saytekin Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey Author-Name: B Sareyyupoglu Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Author-Name: OM Esendal Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Near East University, Nicosia, North Cyprus Title: Isolation and characterisation of Brucella melitensis by bacteriological and molecular methods from livestock in North Cyprus Abstract: In this study, the isolation, biotyping and molecular characterisation of Brucella melitensis from cattle, sheep and goats in North Cyprus are reported on. A total of 319 raw milk samples obtained from seropositive dairy livestock (190 cattle, 74 sheep and 55 goats) and tissue samples including the liver, spleen and abomasal contents obtained from 32 aborted foetal samples (5 cattle, 18 sheep and 9 goats) were analysed for the presence and characterisation of the agent. B. melitensis was isolated and identified from 90 out of 319 (28.2%) milk and 19 out of 32 (59.4%) foetal samples by conventional bacteriological methods. Identification of all 109 isolates was confirmed by using real-time PCR with genus and species-specific primers. Following the preliminary identification, 27 selected isolates representing various counties and herds were further analysed by conventional methods. Twenty (74.1%) isolates were identified as B. melitensis biovar 1 and seven (25.9%) were identified as B. melitensis biovar 3. The Bruce-ladder multiplex PCR assay revealed that all the isolates were field strains. The results of the present study confirmed the presence of B. melitensis in livestock including the cattle population in North Cyprus. Even though the majority of the samples came from seropositive cattle, Brucella abortus was not isolated in the study. The results also revealed the potential public health risk of brucellosis in livestock emphasising the need of implementing strict control and eradication strategies against the disease in animal populations in order to protect human health. Keywords: aborted foetuses, biotyping, Bruce-ladder multiplex PCR, raw milk, real-time PCR Journal: Veterinární medicína Pages: 497-509 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/150/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/150/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202210-0001.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:10:id:150-2021-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D Sperling Author-Workplace-Name: Ceva Sante Animale France, Libourne, France Author-Name: N Isaka Author-Workplace-Name: Ceva Sante Animale France, Libourne, France Author-Name: H Karembe Author-Workplace-Name: Ceva Sante Animale France, Libourne, France Author-Name: J Vanhara Author-Workplace-Name: Ceva Sante Animale France, Libourne, France Author-Workplace-Name: Swine and Ruminant Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: J Vinduska Author-Workplace-Name: ZOD Zichlinek, Zichlinek, Czech Republic Author-Name: N Strakova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: A Kalova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Workplace-Name: Department of Experimental Biology, Faculty of Science, Masaryk University Brno, Brno, Czech Republic Author-Name: IvKolackova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: R Karpiskova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Effect of the vaccination against Shiga toxin 2e in a farm with history of oedema disease, caused by atypical Escherichia coli producing Shiga toxin (STEC) Abstract: Oedema disease of weaned piglets is caused by shigatoxigenic Escherichia coli (STEC), typically harbouring the stx2e gene and F18 adhesins. The aim of this study was to assess the effect of a commercially available oedema disease vaccine on the zootechnical performance, mortality and individual antibiotic treatment in a herd, in which non-typical STEC strains without F18 adhesin have been identified. The zootechnical performance (average daily gain, total weight gain), mortality and individual antibiotic treatment were compared between vaccinated and non-vaccinated control piglets in a monocentric field efficacy study, which was performed using two groups in a parallel, randomised design. A significantly higher average daily gain and total weight gain were recorded in the vaccinated piglets in comparison to the controls. The lower morbidity, mortality and antibiotic treatment in piglets in the vaccine group were not statistically significant. As a conclusion, the positive effect of the vaccination was confirmed in the herd with prevalent STEC not harbouring F18 adhesin. The vaccine was, therefore, also effective against oedema disease caused by such unusual STEC isolates, under the conditions of this study. Keywords: adhesins, pig, STEC, toxoid, whole-genome sequencing (WGS) Journal: Veterinární medicína Pages: 510-518 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/36/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/36/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202210-0002.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:10:id:36-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F Wen Author-Workplace-Name: Department of Animal Production, Henan University of Science and Technology, Luoyang, P.R. China Author-Name: X Liu Author-Workplace-Name: Department of Animal Production, Henan University of Science and Technology, Luoyang, P.R. China Author-Name: K Ding Author-Workplace-Name: Department of Animal Production, Henan University of Science and Technology, Luoyang, P.R. China Author-Name: F Zhang Author-Workplace-Name: Luoyang Xintai Agro-pastoral Technology Co., Ltd., Luoyang, P.R. China Author-Name: J Wang Author-Workplace-Name: Department of Animal Production, Henan University of Science and Technology, Luoyang, P.R. China Title: Dietary Lactobacillus plantarum alleviated Salmonella Typhimurium infection and suppressed Jak/Stat pathway in rabbits Abstract: This study aimed to investigate the effect of a Lactobacillus plantarum DPP8 supplementation on the growth performance, pathogenic invasion, inflammation and pathogen resistance signal pathway in rabbits infected with Salmonella Typhimurium (S. Typhimurium). The treatments included a negative control, a positive control with an S. Typhimurium infection and a positive control plus DPP8 at 106, 108 or 1010 cfu/kg of diet using 300 weaned rabbits. The results showed that supplementing with DPP8 improved (P < 0.05) the feed intake, body weight gain and feed efficiency compared to the positive control. Also, DPP8 decreased (P < 0.05) the S. Typhimurium colonisation and translocation, serum IL-1β, IL-6 and TNF-α, and intestinal mucosa mRNA expressions of the inflammatory mediators Janus kinase (Jak) 2, the signal transducer and activator of transcription (Stat) 1 and 3 at 7 and 14 d post administration. The dose analysis of DPP8 showed linear increases (P ≤ 0.007) in the feed intake and body weight gain, but linear decreases (P ≤ 0.022) in the S. Typhimurium loads, IL-1β, IL-6 and Jak2. It is concluded that Lactobacillus plantarum DPP8 can be used as a supportive probiotic against an S. Typhimurium infection and it possibly plays a direct or indirect role in the downregulation of the Jak/Stat pathway in rabbits. Keywords: growth performance, inflammatory cytokines Journal: Veterinární medicína Pages: 519-526 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/34/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/34/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202210-0003.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:10:id:34-2021-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N Knizatova Author-Workplace-Name: Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: M Massanyi Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: R Rossi Author-Workplace-Name: Department of Veterinary Medicine, University of Milan, Lodi, Italy Author-Name: L Ondruska Author-Workplace-Name: Institute of Farm Animals, Animal Production Research Centre Nitra, Lužianky, Slovak Republic Author-Name: A Kovacik Author-Workplace-Name: Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: K Tokarova Author-Workplace-Name: Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: A Gren Author-Workplace-Name: Institute of Biology, Faculty of Exact and Natural Sciences, Pedagogical University of Cracow, Cracow, Poland Author-Name: G Formicki Author-Workplace-Name: Institute of Biology, Faculty of Exact and Natural Sciences, Pedagogical University of Cracow, Cracow, Poland Author-Name: L Binkowski Author-Workplace-Name: Institute of Biology, Faculty of Exact and Natural Sciences, Pedagogical University of Cracow, Cracow, Poland Author-Name: M Halo Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: N Lukac Author-Workplace-Name: Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: P Massanyi Author-Workplace-Name: Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic Author-Workplace-Name: Institute of Biology, Faculty of Exact and Natural Sciences, Pedagogical University of Cracow, Cracow, Poland Author-Name: F Vizzarri Author-Workplace-Name: Department of Agricultural and Environmental Science, University of Bari Aldo Moro, Bari, Italy Title: The effect of brown seaweed and polyphenol supplementation in male rabbits on the blood profile and antioxidant markers Abstract: Corrigendum in: Vet Med-Czech. 2023 Jan 19;68(1):43. doi: 10.17221/4/2023-VETMEDCurrently, in animal nutrition, the replacement of synthetic substances with natural ones was expected to improve animal health. The aim of the present study was to evaluate the effects of a dietary brown seaweed and plant polyphenol mixture in adult male rabbits on the haematological profile and antioxidant markers. Twenty-four adult male rabbits were divided into three experimental groups receiving a control diet (C) or diets supplemented with 0.3% (T1) and 0.6% (T2) of a feed additive containing brown seaweed (Laminaria spp.) and plant extracts of seaweed origin. The trial lasted for 90 days. A lower potassium concentration was observed at 30 days in the T2 group, compared with the T1 and C groups. An increase in the antioxidant status was observed (P < 0.05) from day 60 of the trial in the rabbits fed diets with an algae-polyphenolic supplement (T1 and T2 groups). Concluding, the diet supplementation of brown seaweed and polyphenol stimulates the antioxidant status of the blood, however, it does not affect the haematological profile. Keywords: algae, antioxidants, biochemical analysis, haematology, rabbit Journal: Veterinární medicína Pages: 527-537 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/26/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/26/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202210-0004.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:10:id:26-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: SG Roh Author-Workplace-Name: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency (APQA), Gimcheon, Republic of Korea Author-Name: J Kim Author-Workplace-Name: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency (APQA), Gimcheon, Republic of Korea Author-Name: S Kim Author-Workplace-Name: National Forensic Service, Wonju, Republic of Korea Author-Name: BJ So Author-Workplace-Name: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency (APQA), Gimcheon, Republic of Korea Author-Name: K Lee Author-Workplace-Name: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency (APQA), Gimcheon, Republic of Korea Title: A case of carboxyatractyloside intoxication by ingestion of the cocklebur (Xanthium strumarium) in a Korean native cow Abstract: A 19-month-old Korean native cow died the following day after consuming new silage. Grossly, the liver showed enlargement, redness, and haemorrhages in all the lobes. Additionally, many of the bur-shaped fruits of the cocklebur (Xanthium strumarium) were found in the gastric contents. The histological evaluation confirmed centrilobular hepatic necrosis. Additionally, carboxyatractyloside (CATR), a material fatal to animals found in the cocklebur, was detected in the gastric contents using high-performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-Q-TOF-MS). Based on the pathological findings and analytical confirmation, CATR intoxication was diagnosed. Therefore, careful feeding and elimination of the cocklebur is essential for minimising economic loss. Keywords: centrilobular hepatic necrosis, cow, silage Journal: Veterinární medicína Pages: 538-543 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/183/2020-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/183/2020-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202210-0005.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:10:id:183-2020-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: SJ Yu Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: HE Kim Author-Workplace-Name: Department of Veterinary Internal Medicine, Konkuk University Veterinary Medical Teaching Hospital, Gwangjin-gu, Seoul, Republic of Korea Author-Name: HJ Han Author-Workplace-Name: Veterinary Emergency Medicine and Critical Care, Konkuk University Veterinary Medical Teaching Hospital, Gwangjin-gu, Seoul, Republic of Korea Author-Name: JH Kim Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Title: Successful management of feline CD4+ CD8+ T-cell mediastinal lymphoma with pericardial effusion Abstract: A 2-year-old, castrated, male Russian blue cat presented with acute dyspnoea, cyanosis, and lethargy. A thoracic radiography revealed a large cranial mediastinal mass; the computed tomography revealed caudal lobe atelectasis of the right lung with pericardial and pleural effusions. The cytodiagnostic tests revealed high-grade CD4+ CD8+ T-cell mediastinal lymphoma as clinical stage Vb; l-asparaginase-cyclophosphamide-doxorubicin-vincristine-prednisolone (l-CHOP)-based chemotherapy was initiated, following which the mass shrunk rapidly; 1 week after the initiation of chemotherapy, the appetite-related and respiratory symptoms improved dramatically, and the pleural and pericardial effusion resolved. The patient remains in complete remission three years after the initiation of the l-CHOP chemotherapy. Therefore, the accurate diagnosis and instantaneous initiation of chemotherapy may resolve life-threatening pleural and pericardial effusions in cats with high-grade aberrant T-cell mediastinal lymphoma. Keywords: cat, flow cytometry, l-CHOP, multi-agent chemotherapy, pleural effusion Journal: Veterinární medicína Pages: 544-551 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/70/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/70/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202210-0006.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:10:id:70-2021-VETMED