Template-Type: ReDIF-Article 1.0 Author-Name: SM Lopresti-Goodman Author-Workplace-Name: Department of Psychology, Marymount University, Arlington, VA, USA Author-Name: N Bensmiller Author-Workplace-Name: Nick Bensmiller Therapy, New York, NY, USA Title: Former laboratory dogs' psychological and behavioural characteristics Abstract: It is becoming increasingly common for laboratories to allow the rehoming of dogs when they are no longer needed. There is no research, however, comparing the characteristics of dogs from laboratories to dogs acquired from more traditional sources for pets. This study used the Canine Behavioral Assessment and Research Questionnaire (C-BARQ) to compare emotional and behavioural characteristics of 100 former laboratory Beagles who were rehomed to a convenience sample of 244 pet Beagles with no known history of laboratory use. Our results revealed that the former laboratory Beagles exhibited increased fearfulness, attention and attachment behaviours, and more abnormal behaviours than the convenience sample, yet were significantly less aggressive, and likely to chase other animals or escape. Importantly, we also found no significant differences in a majority of the everyday behaviours assessed. Our findings demonstrate dogs rehomed from laboratories form strong bonds with their caregivers, and make equally good companions as their non-laboratory counterparts. Our findings may be helpful in designing appropriate rehabilitation programmes, educating laboratory workers and potential adopters, and support the ongoing efforts to offer these dogs for adoption. Keywords: adoption, behaviour, canine, distress Journal: Veterinární medicína Pages: 599-610 Volume: 67 Issue: 12 Year: 2022 DOI: 10.17221/139/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/139/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202212-0001.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:12:id:139-2021-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Q Gong Author-Workplace-Name: Henan University of Science and Technology, Luoyang, P.R. China Author-Name: Y Li Author-Workplace-Name: Henan University of Science and Technology, Luoyang, P.R. China Author-Name: W Zhai Author-Workplace-Name: Henan University of Science and Technology, Luoyang, P.R. China Author-Name: M Niu Author-Workplace-Name: Henan University of Science and Technology, Luoyang, P.R. China Title: Immune responses and protective efficacy of a trivalent combination DNA vaccine based on oprL, oprF and flgE genes of Pseudomonas aeruginosa Abstract: Pseudomonas aeruginosa is an infectious pathogenic bacteria infecting many different species of animals. Currently, it lacks a commercial vaccine. In this study, three monovalent DNA vaccines (poprL, poprF, and pflgE), three bivalent combination DNA vaccines (poprL+poprF, poprL+pflgE, poprF+pflgE), and a trivalent DNA vaccine (poprL+poprF+pflgE) were constructed. Consequently, we immunised chickens with these DNA vaccines and used inactivated vaccines as the positive controls. Then, the immune efficacy was evaluated through serum antibody detection, a lymphocyte proliferation assay, and cytokine concentration determination. Lastly, we assessed the protection rate through a challenge experiment. Following vaccination, the serum antibody levels induced using these DNA vaccines were different due to the different coating antigens. In the trivalent combination DNA vaccine group, we established that the lymphocyte proliferation (SI values), IFN-γ, IL-2, and IL-4 levels were significantly higher than those of the other six DNA vaccine groups and the inactivated vaccine group. However, the protection provided was slightly lower than that of the inactivated vaccine and higher than those of other DNA vaccines. The protection rate of poprL, poprF, pflgE, poprL+poprF, poprL+pflgE, poprF+pflgE, poprL+poprF+pflgE, and the inactivated vaccine were 50, 45, 60, 75, 80, 80, 90, and 95%, respectively. The results of this study indicated the trivalent DNA vaccine based on oprL, oprF and flgE genes represents a promising approach for the prevention of Pseudomonas aeruginosa infections. Keywords: cytokine concentration, poprL+poprF+pflgE, protection rate, serum antibody, SI value Journal: Veterinární medicína Pages: 611-619 Volume: 67 Issue: 12 Year: 2022 DOI: 10.17221/86/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/86/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202212-0002.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:12:id:86-2021-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Kolarova Author-Workplace-Name: Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodňany, Czech Republic Author-Name: E Zuskova Author-Workplace-Name: Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodňany, Czech Republic Author-Name: J Velisek Author-Workplace-Name: Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodňany, Czech Republic Title: Efficacy of a therapeutic bath with selected antiparasitic drugs on a Dactylogyrus anchoratus infection in juvenile common carp (Cyprinus carpio) Abstract: The study aimed to assess the effects of a therapeutic bath of five different antiparasitic drugs, in different baths and durations: fenbendazole (25 mg l-1, 12 h and 2 × 12 h), formaldehyde (0.17 ml l-1, 15 min), ivermectin (0.031 mg l-1, 1 h), mebendazole (1 mg l-1, 12 h) and levamisole (50 mg l-1, 2 h and 3 × 1 h) on the reduction on the intensity and prevalence of a monogenean infection (Dactylogyrus anchoratus) in juvenile carp. The best effect on reducing the parasite number was achieved with the bath in formaldehyde (0.17 ml l-1, 15 min) and fenbendazole (25 mg l-1, 2 × 12 h with 24 h break), where the infection was reduced by more than 90%. Registered veterinary medicinal products (VMPs) with the active substance of fenbendazole can successfully replace the use of unregistered formaldehyde in the treatment of monogenean infections. Keywords: fenbendazole, formaldehyde, ivermectin, levamisole, mebendazole Journal: Veterinární medicína Pages: 620-627 Volume: 67 Issue: 12 Year: 2022 DOI: 10.17221/66/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/66/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202212-0003.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:12:id:66-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Honzlova Author-Workplace-Name: State Veterinary Institute Jihlava, Jihlava, Czech Republic Author-Name: H Curdova Author-Workplace-Name: State Veterinary Institute Jihlava, Jihlava, Czech Republic Author-Name: L Schebestova Author-Workplace-Name: State Veterinary Institute Jihlava, Jihlava, Czech Republic Author-Name: P Bartak Author-Workplace-Name: State Veterinary Institute Jihlava, Jihlava, Czech Republic Author-Name: A Stara Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: J Priborsky Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: M Sandova Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: A Koubova Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: Z Svobodova Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: J Velisek Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Title: Nitrogen factors for rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis) fillets Abstract: Measures for consumer protection against food adulteration and misleading labelling are integrated into EU legislation, including methods for detecting misleading practices. Verification of the meat content is available for marine products, but not for salmonid fish due to the lack of standard nitrogen factors. This study aimed to establish nitrogen factors for rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis). The study analysed 340 fish from Czech fisheries obtained in the summer of 2018-2020. According to the established ISO methods, fillet samples with and without skin were analysed for their nitrogen content (protein), dry matter, ash, and fat. The recommended nitrogen factor for rainbow trout fillets with and without the skin is 3.07 ± 0.12 and 3.06 ± 0.14, respectively, and the nitrogen factor for fat-free rainbow trout fillets with and without the skin is 3.33 ± 0.15 and 3.29 ± 0.15, respectively. The recommended nitrogen factor for brook trout fillets with and without the skin is 3.16 ± 0.10 and 3.12 ± 0.09, respectively, and the nitrogen factor for fat-free brook trout fillets with and without the skin is 3.42 ± 0.13 and 3.36 ± 0.12, respectively. The established nitrogen factors will enable the analysis of the meat content to ensure that consumers purchase correctly described and labelled fish products. Keywords: adulteration, fish-food fraud, fish products, nitrogen factor, salmonid Journal: Veterinární medicína Pages: 628-637 Volume: 67 Issue: 12 Year: 2022 DOI: 10.17221/73/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/73/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202212-0004.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:12:id:73-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N Ayala-Soldado Author-Workplace-Name: Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine, University of Córdoba, Córdoba, Spain Author-Name: AJ Lora-Benitez Author-Workplace-Name: Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine, University of Córdoba, Córdoba, Spain Author-Name: R Mora-Medina Author-Workplace-Name: Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine, University of Córdoba, Córdoba, Spain Author-Name: Molina-Lopez AMª Author-Workplace-Name: Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine, University of Córdoba, Córdoba, Spain Author-Name: JI Artillo-Guimera Author-Workplace-Name: Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine, University of Córdoba, Córdoba, Spain Author-Name: Moyano-Salvago MªR Author-Workplace-Name: Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine, University of Córdoba, Córdoba, Spain Title: Tremorgenic mycotoxicosis in cattle, caused by Claviceps paspali Abstract: Claviceps paspali is a fungus that mainly parasitises Paspalum dilatatum, generating a structure denominated sclerotium, in which indole-diterpenoid alkaloids are isolated. Its action mechanism is related to the inhibition of the gamma-aminobutyric acid receptor. It basically affects bovines, triggering the tremorgenic syndrome, the prevalence of this intoxication being relatively low in Europe. This work describes a clinical case on a cattle farm in Seville (southern Spain), composed of 91 bovines, 60% of which were principally affected with nervous clinical signs. The diagnosis was based on a clinical inspection of the animals, as well as of the presence of paspalum seed heads containing the sclerotia of Claviceps paspali in the plants present in the pastures. The causal agent was identified as being Claviceps paspali, which had parasitised numerous examples of Paspalum paspaloides. The indole-diterpenoid alkaloids produced by Claviceps paspali were identified using ultraviolet-visible spectrophotometry and mass spectrometry. At present, no effective aetiological treatment has been described for poisoning caused by this mycotoxin, so a supportive treatment was administered, and different handling methods were applied, resulting in the complete recovery of the animals. Finally, it was concluded that unusually high humidity and temperature levels for the region triggered the development of the sclerotium generated by this fungus. Keywords: bovine, mycotoxins, Paspalum, sclerotium, tremor Journal: Veterinární medicína Pages: 638-643 Volume: 67 Issue: 12 Year: 2022 DOI: 10.17221/25/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/25/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202212-0005.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:12:id:25-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Nam Author-Workplace-Name: Department of Veterinary Internal Medicine, Konkuk Veterinary Medical Teaching Hospital, Konkuk University, Seoul, Republic of Korea Author-Name: SH Hong Author-Workplace-Name: Helix Animal Medical Center, Songpa-gu, Seoul, Republic of Korea Author-Name: HC Jee Author-Workplace-Name: Helix Animal Medical Center, Songpa-gu, Seoul, Republic of Korea Author-Name: WJ Lee Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea Author-Name: H Myung Author-Workplace-Name: Helix Animal Medical Center, Songpa-gu, Seoul, Republic of Korea Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: DH Kim Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea Title: Surgical management of a metastatic vertebral tumour originating from a mammary adenocarcinoma in a dog Abstract: An 11-year-old spayed female Toy Poodle presented with acute tetraparesis. A small subcutaneous mass was found in the right trunk region, and the magnetic resonance revealed a compressive spinal cord lesion due to an irregular bone proliferation at the third cervical vertebra. After surgical resection of the vertebral lesion, the neurological symptoms improved, and the patient could walk on her own. The excised vertebral and subcutaneous masses were diagnosed as a mammary adenocarcinoma on the histopathological examination, with Ki-67 and HER-2 immunohistochemistry staining. This case report highlights the importance of defining the primary tumours of metastatic vertebral tumours and the necessity of palliative surgery to improve the patient's quality of life. Keywords: bone tumour, canine, mammary cancer, metastasis, palliative surgery Journal: Veterinární medicína Pages: 644-650 Volume: 67 Issue: 12 Year: 2022 DOI: 10.17221/140/2021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/140/2021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202212-0006.txt Handle: RePEc:caa:jnlvet:v:67:y:2022:i:12:id:140-2021-VETMED