Template-Type: ReDIF-Article 1.0 Author-Name: AK Anjani Author-Workplace-Name: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia Author-Name: GR Saragih Author-Workplace-Name: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia Author-Name: H Wihadmadyatami Author-Workplace-Name: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia Author-Name: DL Kusindarta Author-Workplace-Name: Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia Title: Lingual morphology of domesticated Asian small-clawed otters in Yogyakarta, Indonesia Abstract: This study aimed to observe the lingual morphology of the domesticated Asian small-clawed otter, Aonyx cinereus (A. cinereus), from Yogyakarta, Indonesia. Six domesticated A. cinereus adults were obtained from a local otter breeder in Yogyakarta, without regard to gender. The animals were acclimated to the laboratory for one day, followed by macroscopy identification and scanning electron microscopy (SEM) and light microscopy (LM) analysis. Macroscopically, the tongue of domesticated A. cinereus is divided into three parts: the apex, corpus and radix. The apex is the shortest part and can move freely. A median groove is bent along the corpus. Typically, the radix contains circumvallate papillae and the valleculae epiglottis. SEM and LM observations revealed that the lingual morphology of A. cinereus consisted of two types of papillae: mechanic papillae (horny filiform, leaf-like filiform, bifid filiform, trifid filiform, elongated leaf-like filiform, triangular filiform and conical papillae) and gustatory papillae (fungiform and circumvallate papillae). The lingual glands consists of Weber's glands and von Ebner's glands, which secrete acid and neutral mucins. Collagen fibre is found in the lamina propria and muscular layer. In conclusion, the papillae of the Asian short-clawed otter have the same structure as those of other Mustelidae family members. Keywords: Aonyx cinereus, lingual papillae, lingual glands, electron and light microscopy Journal: Veterinární medicína Pages: 91-105 Volume: 68 Issue: 3 Year: 2023 DOI: 10.17221/62/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/62/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202303-0001.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:3:id:62-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: SJ Lu Author-Workplace-Name: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China Author-Name: MY Ma Author-Workplace-Name: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China Author-Name: XG Yan Author-Workplace-Name: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China Author-Name: FJ Zhao Author-Workplace-Name: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China Author-Name: WY Hu Author-Workplace-Name: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China Author-Name: QW Ding Author-Workplace-Name: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China Author-Name: HJ Ren Author-Workplace-Name: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China Author-Name: YQ Xiang Author-Workplace-Name: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China Author-Name: LL Zheng Author-Workplace-Name: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China Title: Development and application of a low-priced duplex quantitative PCR assay based on SYBR Green I for the simultaneous detection of porcine deltacoronavirus and porcine sapelovirus Abstract: Porcine deltacoronavirus (PDCoV) and porcine sapelovirus (PSV) are two viruses that can cause diarrhoea in pigs and bring great economic loss to the pig industry. In this research, a duplex real-time quantitative polymerase chain reaction (qPCR) assay based on SYBR Green І was developed to simultaneously detect PDCoV and PSV. No specific melting peaks were found in other porcine diarrhoea-associated viruses, indicating that the method developed in this study had good specificity. The detection limits of PDCoV and PSV were 1.0 × 101 copies μl-1 and 1.0 × 102 copies μl-1, respectively. The duplex real-time qPCR assay tested two hundred and three (203) intestinal and faecal samples collected from diarrhoeal and asymptomatic pigs. The positive rates of PDCoV and PSV were 20.2% and 23.2%, respectively. The co-infection rate of PDCoV and PSV was 13.8%. To evaluate the accuracy of the developed method, conventional PCR and singular TaqMan real-time qPCR assays for PDCoV/PSV were also used to detect the samples. The results showed that the duplex real-time qPCR assay was consistent with the singular assays, but its sensitivity was higher than conventional PCR methods. This duplex real-time qPCR assay provides a rapid, sensitive and reliable method in a clinic to simultaneously detect PDCoV and PSV. Keywords: duplex real-time qPCR, PDCoV, PSV Journal: Veterinární medicína Pages: 106-115 Volume: 68 Issue: 3 Year: 2023 DOI: 10.17221/79/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/79/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202303-0002.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:3:id:79-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: CJ Lien Author-Workplace-Name: Graduate Institute of Veterinary Clinical Sciences, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan, ROC Author-Name: SL Wang Author-Workplace-Name: Graduate Institute of Veterinary Clinical Sciences, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan, ROC Title: The impact of urine storage methods on the results of quantitative bacterial culture in dog and cat Abstract: Quantitative bacterial culture (QBC) is the gold standard for determining urinary tract infections. However, the majority of urine samples were sent to a lab for further bacterial culture. Therefore, the storage condition was vital to maintain the quality and accuracy of the urine samples. The main objective of our study was to examine the urine QBC outcomes of (1) immediate culture, (2) culture after urine sample storage at ambient temperature for 24 h, and (3) culture after urine sample storage at 4 °C for 24 hours. There were 49 feline samples and 30 canine samples included in this study. All QBC samples kept at ambient temperature and refrigerator were consistent with immediate QBC in cats. Eight positive results from immediate QBC were in accordance with refrigerated results in dogs. There were ten positive results in the room-temperature sample with two false-positive results. Our study showed that storing conditions at room temperature or refrigeration for 24 h does not impact the results of QBC in cat urine samples. For dog samples, chilled samples have a higher accuracy rate than room temperature samples, although the overall agreement was still satisfactory. Keywords: canine, feline, urinalysis, urinary tract infections Journal: Veterinární medicína Pages: 116-121 Volume: 68 Issue: 3 Year: 2023 DOI: 10.17221/111/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/111/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202303-0003.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:3:id:111-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M Ha Author-Workplace-Name: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: YD Suh Author-Workplace-Name: Seoul Wildlife Center, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: S Ahmed Author-Workplace-Name: Seoul Wildlife Center, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: DN Lee Author-Workplace-Name: Seoul Wildlife Center, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: JH Han Author-Workplace-Name: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Author-Name: YK Kim Author-Workplace-Name: The Bone Animal Medical Center, Nam-gu, Busan, Republic of Korea Author-Name: SC Yeon Author-Workplace-Name: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea Title: Serum biochemical reference interval determination in wild Siberian weasel (Mustela sibirica) Abstract: Determining reference intervals (RI) is a valuable asset for assessing the health of wildlife species. This is the first study to establish serum biochemical RIs in Siberian weasels. Forty-two healthy free-ranging Siberian weasels were captured live and brought to Seoul Wildlife Center between June 2021 and August 2022. Blood samples from 42 healthy Siberian weasels of both sexes were used to calculate RIs. An automated analyser was used to perform serum biochemistry profiles. The American Society for Veterinary Clinical Pathology recommendations were used to calculate a nonparametric RI with 90% confidence intervals. The RIs of albumin, total protein, globulin, calcium, glucose, blood urea nitrogen, phosphorus, amylase, cholesterol, alanine aminotransferase, total bilirubin, alkaline phosphatase, creatinine, and creatine kinase were determined. The RIs established in this study will serve as a good starting point for analysing serum biochemical data in Siberian weasels. Keywords: automated analyser, free-ranging Siberian weasel, serum biochemistry profiles, wildlife conservation, wildlife species Journal: Veterinární medicína Pages: 122-128 Volume: 68 Issue: 3 Year: 2023 DOI: 10.17221/102/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/102/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202303-0004.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:3:id:102-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H Zborilova Author-Workplace-Name: Avian and Exotic Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: J Dietz Author-Workplace-Name: Department of Pathology, LABOKLIN Laboratory for Clinical Diagnostics GmbH & Co. KG, Bad Kissingen, Germany Author-Name: KO Heckers Author-Workplace-Name: Department of Pathology, LABOKLIN Laboratory for Clinical Diagnostics GmbH & Co. KG, Bad Kissingen, Germany Author-Name: R Dvorakova Author-Workplace-Name: Department of Imaging Methods, Dog and Cat Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: Z Knotek Author-Workplace-Name: Avian and Exotic Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: E Cermakova Author-Workplace-Name: Avian and Exotic Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Title: Malignant ovarian dysgerminoma in a 16-year-old leopard gecko (Eublepharis macularius) Abstract: The 16-year-old female leopard gecko (Eublepharis macularius) was presented with distended coelom and cachexia. Examination of the faecal sample ruled out the presence of protozoan parasites. A radiographic examination confirmed the presence of radiopaque foreign material in the intestine. The conservative treatment with tramadol, butylscopolamine, famotidine, vitamin B complex, and supportive fluid therapy with Hartmann solution and Duphalyte, was performed for 14 days. Ultrasonographic examination revealed the presence of a large mass adherent to the liver (with hypoechoic regions), a thin-walled cystic structure close to the liver, and coelomic effusion. Surgical exploration revealed a large mass on the right ovary. The unilateral (right) ovariectomy was performed. Histologic examination of the mass revealed dysgerminoma with an invasion of the ovarian bursa and blood vessels. Nine months after the surgery the patient was active and doing well. In reptiles, dysgerminoma is an uncommon type of neoplasia. To the best of our knowledge, this is the first case of dysgerminoma tumour diagnosed intravitally and treated successfully in lizards. Keywords: histopathology, lizards, malignant neoplasia, surgery, reptiles Journal: Veterinární medicína Pages: 129-134 Volume: 68 Issue: 3 Year: 2023 DOI: 10.17221/107/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/107/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202303-0005.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:3:id:107-2022-VETMED