Template-Type: ReDIF-Article 1.0 Author-Name: B Yanmaz Author-Workplace-Name: Department of Public Health, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkiye Author-Name: EK Ozgen Author-Workplace-Name: Department of Public Health, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkiye Title: Detection of Coxiella burnetii and characterisation by multiple-locus variable-number tandem repeat analysis in bovine bulk tank milk samples Abstract: Coxiella burnetii is the aetiological agent of Q fever, which is highly prevalent in Turkiye, but information on the genetic profiles of the bacterium is limited. This study aimed to investigate the presence of C. burnetii in bovine bulk tank milk (BTM) samples by polymerase chain reaction (PCR) and to investigate the genotypes by means of multiple-locus variable-number tandem repeat analysis (MLVA). A total of 25 markets that sold raw cow's milk were analysed by conventional PCR analysis. An MLVA analysis was performed at six loci, namely MS23, MS24, MS27, MS28, MS33, and MS34, to determine the genotypic variations of C. burnetii found in the positive DNA samples. The DNA of C. burnetii was detected in 16% of the BTM samples. The C. burnetii strains identified in the bovine milk samples collected in this study were found to belong to the same genotypic group as those detected in the bovine milk samples gathered in Greece. As a result, both the presence and genotyping studies of C. burnetii on the BTM samples in Turkiye will contribute to the determination of the geographical distribution of the agent. Keywords: genotyping, Q fever, raw milk, Turkiye Journal: Veterinární medicína Pages: 185-190 Volume: 68 Issue: 5 Year: 2023 DOI: 10.17221/87/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/87/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202305-0001.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:5:id:87-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Y Yoo Author-Workplace-Name: Department of Food and Nutrition, Sookmyung Women's University, Seoul, Republic of Korea Author-Name: J Lee Author-Workplace-Name: Department of Poultry Science, University of Georgia, Athens, GA, USA Author-Name: J Cho Author-Workplace-Name: Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea Author-Name: Y Yoon Author-Workplace-Name: Risk Analysis Research Center, Sookmyung Women's University, Seoul, Republic of Korea Title: Antimicrobial properties of Limosilactobacillus reuteri strains for control of Escherichia coli and Salmonella strains, diarrhoea cause in weaning pigs Abstract: This study aimed to use lactic acid bacteria isolated from piglet faeces to develop probiotics, allowing for the effective control of Escherichia coli and Salmonella. Lactic acid bacteria were isolated from the faeces of suckling piglets and identified by 16S rRNA sequencing, then examined for haemolysis; gelatinase activity; and resistance to acid, bile, and pancreatin. The antimicrobial activity of selected lactic acid bacteria isolates was examined for 8 E. coli and 7 Salmonella strains. One-hundred and sixty-four lactic acid bacteria isolates were identified from 118 piglet faecal samples, and 13 lactic acid bacteria isolates were selected from analyses of haemolysis; gelatinase activity; and resistance to acid, bile, and pancreatin. Of the selected 13 lactic acid bacteria isolates, Limosilactobacillus reuteri PF20-3 and PF30-3 strains had the highest antibacterial activity against E. coli and Salmonella. Keywords: antibiotic resistance, feed additive, gut health, gut microbiota, probiotics Journal: Veterinární medicína Pages: 191-199 Volume: 68 Issue: 5 Year: 2023 DOI: 10.17221/112/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/112/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202305-0002.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:5:id:112-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F Liao Author-Workplace-Name: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, P.R. China Author-Name: T Bao Author-Workplace-Name: Guizhou Vocational College of Agriculture, Qingzhen, P.R. China Author-Name: G Tao Author-Workplace-Name: Qiandongnan Center for Animal Disease Control and Prevention, Kaili, Guizhou, P.R. China Author-Name: Y Hu Author-Workplace-Name: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, P.R. China Author-Name: C Han Author-Workplace-Name: Qiandongnan Center for Animal Disease Control and Prevention, Kaili, Guizhou, P.R. China Title: In vitro evaluation of the composition and acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites Abstract: In veterinary medicine, natural products provide an alternative to chemical agents for mite management. In the present study, the acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites was examined. The chemical composition of the extract was determined using liquid chromatography-mass spectrometry (LC-MS) analysis. The ethyl acetate extract was found to be extremely toxic to mites at a concentration of 100 mg/ml (m/v), killing all S. scabiei within two hours. The median lethal time (LT50) values for ethyl acetate extract concentrations of 25, 50, and 100 mg/ml against S. scabiei were 1.706, 1.204, and 0.750 h, respectively. The median lethal dosage (LC50) for S. scabiei was 19.14 mg/ml at two hours. The chemical composition of the ethyl acetate extract was evaluated using LC-MS, showing that the major components were schaftoside (8.259%), carnosol (6.736%), prostaglandin A2 (5.94%), 13(S)-HpOTrE (4.624%), nandrolone (4.264%), 1H-indole-3-carboxaldehyde (4.138%), 9-oxoODE (3.206%), and stearidonic acid (2.891%). In conclusion, these findings indicate that Urtica fissa contains promising new acaricidal compounds capable of successfully controlling animal mites. Keywords: acaricidal properties, LC-MS, sarcoptic mange, Urtica fissa Journal: Veterinární medicína Pages: 200-207 Volume: 68 Issue: 5 Year: 2023 DOI: 10.17221/6/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/6/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202305-0003.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:5:id:6-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B Celik Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye Author-Name: B Ergul Author-Workplace-Name: Ambarli Veterinary Border Control Point Directorate, Republic of Turkiye Ministry of Agriculture and Forestry, Istanbul, Turkiye Author-Name: AI Kekec Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye Author-Name: B Halac Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye Author-Name: B Maslak Author-Workplace-Name: Institute of Graduate Studies, Istanbul University - Cerrahpasa, Avcilar/Istanbul, Turkiye Author-Name: B Diren Sigirci Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye Author-Name: B Basaran Kahraman Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye Author-Name: AF Bagcigil Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye Author-Name: K Metiner Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye Author-Name: S Ak Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, Buyukcekmece/Istanbul, Turkiye Title: Beta-lactam, aminoglycoside, and quinolone resistance in Escherichia coli strains isolated from shrimps and mussels in the Marmara Sea Abstract: The purpose of the study was to examine the prevalence of Escherichia coli in shrimps and mussels, and to determine the distribution of β-lactam, aminoglycoside, quinolone, and multi-drug resistance phenotypically and genotypically in E. coli isolates obtained from mussels and shrimps in Istanbul. Faecal samples were collected from mussels (n = 96) and shrimps (n = 96) from the Marmara Sea coastline and fish markets in Istanbul. For the detection of antibiotic susceptibilities, seven antibiotic groups were used. β-lactamase, aminoglycoside, and quinolone genes were also determined. A total of 34 (17.7%, 15 shrimps, and 19 mussels) E. coli were isolated, and 17 (50%) were found to be resistant to one or more antimicrobials. The highest resistance was seen against aminoglycosides with 11 isolates (32.35%), followed by quinolones with 10 isolates (29.41%) and extended-spectrum β-lactamase (ESBL) with 4 isolates (11.76%). Multi-drug resistance was detected in 5 isolates (14.7%) from 3 shrimp and 2 mussel samples. The prevalence of ESBL genes was demonstrated at 3.84% in mussels and shrimp samples. There were no AmpC and carbapenemase-producing genes. These samples harbored blaCTX-M-1 (n = 3) and blaTEM (n = 4). Ten isolates were resistant to aminoglycosides genotypically. Resistance genes detected were strB in 2 isolates, aadA in 5, strB and aadA together in 3, ANT('')-Ia, aphA1 and aphA2 simultaneously in 3, aphA1 in 1, aac(3)-IIa in 1 isolate. aac(6')-Ib-cr gene was detected in only one of 10 phenotypically resistant isolates to quinolones. Keywords: aquatic animals, E. coli, ESBL, multiple drug resistance Journal: Veterinární medicína Pages: 208-217 Volume: 68 Issue: 5 Year: 2023 DOI: 10.17221/105/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/105/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202305-0004.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:5:id:105-2022-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Cahova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: J Blahova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: L Plhalova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: P Marsalek Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: V Doubkova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: M Hostovsky Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: L Divisova Author-Workplace-Name: Simulation Centre, Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: J Mares Author-Workplace-Name: Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agrosciences, Mendel University Brno, Brno, Czech Republic Author-Name: C Faggio Author-Workplace-Name: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy Author-Name: Z Svobodova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Title: Long-term exposure to polycyclic musk tonalide - A potential threat to juvenile zebrafish (Danio rerio)? Abstract: Polycyclic musk compounds are commonly used in personal care products to replace expensive natural fragrances. Due to their huge consumption, they have become a part of the aquatic environment. In the present study, a two-month exposure effect of tonalide on juvenile zebrafish (Danio rerio) was investigated. We determined the vitellogenin concentration to define the potential endocrine-disrupting effect of tonalide and also analysed selected indices to evaluate the induction of oxidative stress. The environmentally relevant concentration of tonalide (i.e., 500 ng/l) caused a significant decrease in the catalase activity (P < 0.05) and a significant increase (P < 0.05) in the lipid peroxidation. An increasing lipid peroxidation was also recorded for the highest concentration group tested (i.e., 50 000 ng/l). On the other hand, no significant changes were recorded in vitellogenin in all the exposed groups. Thus, based on these results, we have not demonstrated the endocrine-disrupting activity of tonalide in zebrafish. The results of the oxidative stress indices showed a significant impairment of the antioxidant defence after the two-month tonalide exposure, which could indicate part of the adaptive response to the tonalide toxicity. Keywords: aquatic contamination, endocrine disruptor, oxidative stress, personal care products, vitellogenin Journal: Veterinární medicína Pages: 218-224 Volume: 68 Issue: 5 Year: 2023 DOI: 10.17221/40/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/40/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202305-0005.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:5:id:40-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: MJ Jung Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: KY Yoon Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: YM Kim Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: JS Lee Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: JW Choi Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: JH Kim Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Name: HY Yoon Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Workplace-Name: KU Center for Animal Blood Medical Science, Gwangjin-gu, Seoul, Republic of Korea Author-Name: JH Kim Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea Author-Workplace-Name: KU Center for Animal Blood Medical Science, Gwangjin-gu, Seoul, Republic of Korea Title: Long-term adjuvant metronomic chemotherapy in a dog with recurrent maxillofacial osteosarcoma Abstract: Osteosarcoma (OSA) is the most common malignant bone tumour in dogs; however, OSA of the maxilla is uncommon compared to appendicular OSA. Oral melanoma also commonly occurs in dogs with frequent distant metastasis. The role of adjuvant chemotherapy has been questioned in maxillary OSA and melanoma. A 17-year-old English Cocker Spaniel was referred with a growing mass on the right maxilla and a right lower lip mass. Osteosarcoma was diagnosed after partial maxillectomy, and the right lower lip mass was diagnosed as oral melanoma. Metronomic chemotherapy (MC) was performed, and the number of doses was tapered due to side effects at 5 weeks after initiation of MC. After 130 weeks of MC, chemotherapy was suspended due to kidney disease. After the suspension of chemotherapy, findings suggesting recurrence and metastasis were detected. The dog suddenly died 193 weeks after surgery, which was 8-14 times longer than the expected survival time. To the best of our knowledge, this is the first case report of successful long-term combination therapy, including surgery and MC, in a dog with maxillary OSA and lip melanoma. Our results show that the survival time can be greatly extended if MC is performed with proper management. Keywords: osteosarcoma, melanoma, cyclophosphamide, piroxicam, adverse effects, survival time Journal: Veterinární medicína Pages: 225-230 Volume: 68 Issue: 5 Year: 2023 DOI: 10.17221/43/2022-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/43/2022-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202305-0006.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:5:id:43-2022-VETMED