Template-Type: ReDIF-Article 1.0 Author-Name: A Rahman Author-Workplace-Name: Department of Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Sub-campus, Jhang, Punjab, Pakistan Author-Name: M Kashif Author-Workplace-Name: Department of Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Sub-campus, Jhang, Punjab, Pakistan Author-Name: A Nasir Author-Workplace-Name: Department of Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Sub-campus, Jhang, Punjab, Pakistan Author-Name: S Ehtisham-ul-Haque Author-Workplace-Name: Department of Basic Sciences, University of Veterinary and Animal Sciences Lahore, Sub-campus, Jhang, Punjab, Pakistan Author-Name: H Ullah Author-Workplace-Name: Department of Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Sub-campus, Jhang, Punjab, Pakistan Author-Name: A Sikandar Author-Workplace-Name: Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore, Sub-campus, Jhang, Punjab, Pakistan Author-Name: I Ahmed Author-Workplace-Name: Department of Clinical Sciences, Bahauddin Zakariya University, Multan, Punjab, Pakistan Author-Name: AU Rehman Author-Workplace-Name: Faculty of Veterinary and Animal Sciences, Gomal University, D.I. Khan, Khyber Pakhtunkhwa, Pakistan Author-Name: MA Saeed Author-Workplace-Name: Faculty of Veterinary and Animal Sciences, Gomal University, D.I. Khan, Khyber Pakhtunkhwa, Pakistan Author-Name: MW Nazar Author-Workplace-Name: Department of Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Sub-campus, Jhang, Punjab, Pakistan Author-Name: M Rizwan Author-Workplace-Name: Department of Clinical Sciences, Bahauddin Zakariya University, Multan, Punjab, Pakistan Author-Name: S Saher Author-Workplace-Name: Department of Clinical Sciences, Bahauddin Zakariya University, Multan, Punjab, Pakistan Author-Name: A Abbas Author-Workplace-Name: Department of Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Sub-campus, Jhang, Punjab, Pakistan Title: Seroprevalence and haemato-biochemical effects of bovine leucosis in buffalo, Punjab, Pakistan Abstract: Enzootic bovine leucosis is caused by bovine leukaemia virus (BLV), a Deltaretrovirus belonging to the family Retroviridae. BLV causes huge economic losses to the dairy industry in the form of decreased milk production, premature culling, and poor reproductive performance of the animals. The aim of the present study was to determine the seroprevalence of BLV infection in buffalo in two districts of Punjab, Pakistan. A total of 384 samples were collected and analysed using a commercial indirect enzyme-linked immunosorbent assay (ELISA) to investigate the seroprevalence of BLV through the detection of the anti-BLV gp51 antibody. A predesigned data questionnaire proforma was employed to find out the association of risk factors with disease. Overall, 18.2% of buffaloes were seropositive for BLV in the study population. The results revealed a significant association (P < 0.05) of age with BLV infection. Furthermore, milk yield and pregnancy had a significant association with the seroprevalence of BLV infection in buffalo whereas no significant association was found with sex, breeding, and health status. Biochemical and oxidative stress markers revealed a significant decrease in liver enzymes alanine transaminase (ALT) and aspartate transaminase (AST), glutathione peroxidase (GPX), and superoxide dismutase (SOD) in seropositive animals as compared to healthy animals. It is concluded that BLV has a considerable prevalence in buffalo in Punjab, Pakistan and there is a dire need to investigate the disease epidemiology at both national and international levels and strategies should be developed to implement an effective control program. Keywords: biochemical parameters, bovine leucosis, ELISA, seroprevalence Journal: Veterinární medicína Pages: 385-391 Volume: 68 Issue: 10 Year: 2023 DOI: 10.17221/57/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/57/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202310-0001.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:10:id:57-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Chai Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Author-Name: X Long Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Author-Name: P Wu Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Author-Name: J Wang Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Author-Name: X Wu Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Author-Name: Z Tu Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Author-Name: M Wei Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Author-Name: Z Guo Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Author-Name: T Zhang Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Author-Name: L Chen Author-Workplace-Name: Chongqing Academy of Animal Science, Chong Qing, Rongchang, P.R. China Author-Workplace-Name: National Center of Technology Innovation for Pigs, Chong Qing, Rongchang, P.R. China Title: Lactobacillus sp. participated in the adaptation of Rongchang piglets to cold stress Abstract: Rongchang piglets were easily induced to cold stress and diarrhoea in the winter when raised in an open hog house. However, they also gradually recovered under mid-cold stress. Other studies have suggested gut microbiome might be involved in the host energy metabolism to relieve stress. To study how to adapt Rongchang piglets to cold stress by gut microbiome, thirty Rongchang piglets were randomly divided into a mild cold stress group and a control group for 30 consecutive days. The findings revealed that the piglets had low growth performance and a high diarrhoea rate and mortality rate during the first half of the cold treatment, but subsequently stabilised. The level of cortisol (COR) also displayed a similar trend. In the mild cold stress group, the relative abundance of Muribaculaceae significantly increased on day 15, and the predominant bacterial on day 30 was Lactobacillus sp. Our results indicated that the Rongchang piglet's production performance and health were impaired at the start of the mild cold stress. However, as time passed, the body could progressively adapt to the low temperature, and Lactobacillus sp. participated in this process. This study provides new insight into how to alleviate health damage caused by cold stress. Keywords: energy metabolism, gut microbiome, low temperature, production performance Journal: Veterinární medicína Pages: 392-402 Volume: 68 Issue: 10 Year: 2023 DOI: 10.17221/54/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/54/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202310-0002.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:10:id:54-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M Kuricova Author-Workplace-Name: Small Animal Clinic, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: J Fuchs Author-Workplace-Name: Small Animal Clinic, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: T Liptak Author-Workplace-Name: Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: F Korim Author-Workplace-Name: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: N Surin Hudakova Author-Workplace-Name: Moscow State Academy of Veterinary Medicine and Biotechnology MVA named after K. I. Skryabin, Ulitsa Akademika Skryabina, Moscow, Russia Author-Name: B Bhattarai Author-Workplace-Name: Zoltan Kerekes, VetScan, Veterinary Imaging Center, Budapest, Hungary Author-Name: Z Kerekes Author-Name: V Revajova Author-Workplace-Name: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Title: Spinal cord haemangiosarcoma in one dog - Case report Abstract: A 5-year-old intact female Shih Tzu was presented with acute onset of hind leg paralysis. The neurologic examination revealed severe T3-L3 myelopathy. The differential diagnoses included degenerative, anomalous, traumatic, inflammatory, vascular, metabolic, and neoplastic changes. The results of the paraclinical examinations and diagnostic imaging narrowed the list of differential diagnoses and, along with the patient's deteriorating condition, led to the owner's decision to euthanise the dog. The histologic findings of the spinal cord specimens indicated a tumour of the blood vessels formed by the proliferation of endothelial cells, which may present as either capillary or cavernous structures. In this case, the tumour was a capillary-type haemangiosarcoma. The primary site of proliferation could not be determined in this case because no mass formation was noted while performing the necropsy. Keywords: canine, intramedullary, paralysis, tumour Journal: Veterinární medicína Pages: 412-418 Volume: 68 Issue: 10 Year: 2023 DOI: 10.17221/60/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/60/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202310-0003.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:10:id:60-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Kruzicova Author-Workplace-Name: Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences in Brno, Brno, Czech Republic Author-Name: M Chalupova Author-Workplace-Name: Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences in Brno, Brno, Czech Republic Author-Name: G Kuzminova Author-Workplace-Name: Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences in Brno, Brno, Czech Republic Author-Name: T Parak Author-Workplace-Name: Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences in Brno, Brno, Czech Republic Author-Name: J Klusakova Author-Workplace-Name: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic Author-Name: T Sopuch Author-Workplace-Name: Histos, Ltd., Brno, Czech Republic Author-Name: P Suchy Author-Workplace-Name: Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences in Brno, Brno, Czech Republic Title: Effect of novel carboxymethyl cellulose-based dressings on acute wound healing dynamics Abstract: The clinical implications and efficacy of newly developed modified cellulose materials were evaluated in an acute wound animal model. In the current study, sixty male rats were divided into four groups. A full-thickness circular excision wound was created in the suprascapular area. Newly developed matrices (acidic partially carboxymethylated cellulose; acidic partially carboxymethylated cellulose impregnated with a povidone-iodine solution) were applied in two test groups, while fifteen animals were used as a control group without any primary dressing. Aquacel Ag, a clinically used dressing, was selected as the reference material. To compare the efficacy in vivo, the wound size and production of selected cytokines and growth factors (TNF-α, TGF-β1, and VEGF), which play a key role in the healing process, were measured at two, seven, and fourteen days after surgery. The activity of matrix metalloproteinases 2 and 9, which actively participate in cell signalling and are essential for tissue remodelling, was determined in wound tissue by gelatin zymography. A positive effect of the newly developed dressing materials on the healing process, tissue granulation, and wound re-epithelialisation was demonstrated. Keywords: cytokines, growth factors, wound dressing, Wistar rat Journal: Veterinární medicína Pages: 403-411 Volume: 68 Issue: 10 Year: 2023 DOI: 10.17221/89/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/89/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202310-0004.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:10:id:89-2023-VETMED