Template-Type: ReDIF-Article 1.0 Author-Name: J Haluskova Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: B Holeckova Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: L Kokulova Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: M Galdikova Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: J Bucan Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: V Schwarzbacherova Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: S Sedlakova Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Title: Detection of the T1640C RYR1 mutation indicating malignant hyperthermia in dogs Abstract: Malignant hyperthermia (MH) is a clinical syndrome exhibiting elevation of expired carbon dioxide, hyperthermia, muscle rigidity, rhabdomyolysis, acidosis and hyperkalaemia, as well as cardiac dysrhythmia and renal failure. The syndrome manifests itself as a response to anaesthetic agents, such as e.g., halothane, desflurane, and succinylcholine. Depending on the animal species, MH is characterised by autosomal dominant or recessive inheritance, and so far two genes have been identified whose mutations can be linked to MH: RYR1 and CACNA1S. In different species, various mutations of the RYR1 gene have been described which may underlie MH. One of these mutations in dogs is T1640C, which results in the substitution of alanine for valine of the amino acid 547 (V547A) in the RYR1 protein. In our work, we aimed to investigate MH at the DNA level by identifying the T1640C mutation in a group of 50 dogs. For this purpose we used the PCR-RFLP technique, and in six dogs also direct sequencing of PCR products and subsequent comparison of their sequences with the RYR1 gene sequence in an online database. The results of our study show that none of the dogs analysed had any mutant allele of the RYR1 gene, indicating that none should be affected by MH. Keywords: allele, anaesthesia, disease, gene, sequencing Journal: Veterinární medicína Pages: 428-434 Volume: 68 Issue: 11 Year: 2023 DOI: 10.17221/46/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/46/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202311-0001.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:11:id:46-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V Sladecek Author-Workplace-Name: Ptacy s.r.o, Valasska Bystrice, Czech Republic Author-Name: D Senk Author-Workplace-Name: Ptacy s.r.o, Valasska Bystrice, Czech Republic Author-Name: P Stolar Author-Workplace-Name: Ptacy s.r.o, Valasska Bystrice, Czech Republic Author-Name: J Bzdil Author-Workplace-Name: Ptacy s.r.o, Valasska Bystrice, Czech Republic Author-Workplace-Name: Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: O Holy Author-Workplace-Name: Science and Research Centre, Faculty of Health Sciences, Palacky University Olomouc, Olomouc, Czech Republic Title: Predominance of Acinetobacter pseudolwoffii among Acinetobacter species in domestic animals in the Czech Republic Abstract: The aim of this study was to map the spectrum of microorganisms belonging to the genus Acinetobacter in domestic animals with a specific focus on the prevalence of Acinetobacter pseudolwoffii. Additionally, the susceptibility of isolates to antimicrobial agents was determined. In the period from January 1, 2014, to August 31, 2015, a total of 9 544 samples originating from gross lesions and pathological processes of animals exhibiting clinical symptoms of the disease were examined across 41 districts in the Czech Republic. The examinations were carried out using culture methods involving meat-peptone blood agar and Endo agar under aerobic conditions at a temperature of 37 ± 1 °C for 18-24 hours. Isolates were confirmed using molecular phenotypic method MALDI-TOF MS with the MBT Compass Library Revision L 2020 covering 3 239 species/entries (9 607 MSP) from Bruker Daltonics company. Out of the 108 isolates (prevalence 1.13%), 14 species of Acinetobacter spp. were identified, with 5 isolates remaining unclassified as species. A. pseudolwoffii was the predominant species isolated in 25 cases (prevalence 0.26%). Antimicrobial susceptibility was determined for 12 antimicrobials by the disc diffusion method, with A. pseudolwoffii isolates exhibiting the lowest susceptibility to ceftazidime (32%) and co-trimoxazole (60%). Keywords: organ;, prevalence;, pathogenicity;, species;, susceptibility;, veterinary Journal: Veterinární medicína Pages: 419-427 Volume: 68 Issue: 11 Year: 2023 DOI: 10.17221/65/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/65/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202311-0002.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:11:id:65-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M Zeghdoudi Author-Workplace-Name: Department of Veterinary Sciences, Chadli Bendjedid University, El Tarf, Algeria Author-Workplace-Name: Laboratory ESSPRETCADS, Chadli Bendjedid University, El Tarf, Algeria Author-Name: M Latifa Author-Workplace-Name: Department of Veterinary Sciences, Chadli Bendjedid University, El Tarf, Algeria Author-Workplace-Name: Laboratory ESSPRETCADS, Chadli Bendjedid University, El Tarf, Algeria Author-Name: M Seloua Author-Workplace-Name: Department of Veterinary Sciences, Chadli Bendjedid University, El Tarf, Algeria Author-Workplace-Name: Laboratory ESSPRETCADS, Chadli Bendjedid University, El Tarf, Algeria Author-Name: S Rima Author-Workplace-Name: Department of Veterinary Sciences, Chadli Bendjedid University, El Tarf, Algeria Author-Name: T Mardja Author-Workplace-Name: Department of Veterinary Sciences, Chadli Bendjedid University, El Tarf, Algeria Author-Name: L Aoun Author-Workplace-Name: Department of Veterinary Sciences, Chadli Bendjedid University, El Tarf, Algeria Author-Workplace-Name: Laboratory ESSPRETCADS, Chadli Bendjedid University, El Tarf, Algeria Title: Updating of epidemiological and pathological features of Marek's disease in laying hens and broilers Abstract: Marek's disease (MD) is a huge problem for researchers due to the significant losses in bird flocks, but more importantly, the virus's mutagenic potential. The purpose of this study was to describe non-classical gross lesions observed in broilers and laying hens that suggest the disease emergence and re-emergence. The survey was conducted on 10 broiler and 4 laying hen flocks. All of the dead birds were necropsied in order to obtain a comprehensive diagnosis of lesions, analysing both macroscopic and microscopic alterations. Marek's disease occurred in 80% of cases in broilers and 100% of cases in layer hens. The disease struck 26-day-old broilers and hens at 2 weeks of age, causing a total mortality of 6% and 15%, respectively. There were no clinical indications of the classical neurological form of the disease in either rearing type, and tumour lesions were mostly detected in the liver, spleen, and ovarian follicles in layers, and in the proventriculus in broilers. These findings demonstrated that MD is widespread and that its resurgence is primarily manifested in visceral rather than neurological manifestations. Despite MD immunization, biosecurity remains critical. Keywords: histopathology,  lesions, MD,  re-emergence Journal: Veterinární medicína Pages: 443-448 Volume: 68 Issue: 11 Year: 2023 DOI: 10.17221/75/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/75/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202311-0003.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:11:id:75-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D Han Author-Workplace-Name: Choi Youngmin Animal Medical Center, Seoul, Republic of Korea Author-Name: DI Jung Author-Workplace-Name: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea Title: Gallbladder atrophy associated with pancreatitis: Clinical and advanced imaging diagnosis in a dog Abstract: Gallbladder atrophy (GBA) is characterised by a reduction in the size and volume of the gallbladder. In human medicine, it is well-established that GBA frequently occurs together with pathologies affecting the gallbladder and pancreas. However, to the best of our knowledge, there is currently a dearth of reported cases of GBA in dogs within the veterinary field. In this study, we present a case report of GBA in a 7-year-old Yorkshire Terrier. The diagnosis of GBA was confirmed using abdominal ultrasonography and advanced imaging techniques, including computed tomography, which were performed over a 4-year period. The patient initially presented with predominantly gastrointestinal symptoms, which were subsequently diagnosed and treated as pancreatitis. Concurrently, a gallbladder nodule and an anomalous structure suspected to be cholelithiasis were identified. However, during the 4-year follow-up, the gallbladder structure regressed, leaving only the presence of the gallbladder nodule. Notably, cholecystectomy was not performed, and apart from pancreatitis-related symptoms, the patient did not show any gallbladder-related problems throughout the spontaneous atrophic process. Based on these findings, we propose that the observed GBA was likely induced by cholecystitis associated with pancreatitis. This case underscores the significance of considering GBA as a potential diagnosis in canine patients presenting with pancreatitis and gastrointestinal symptoms. Furthermore, it highlights the value of comprehensive diagnostic imaging in accurately determining the underlying cause of these symptoms. Keywords: cholecystitis, cholelithiasis, computed tomography, gastroinetstinal symptom, nodule Journal: Veterinární medicína Pages: 435-442 Volume: 68 Issue: 11 Year: 2023 DOI: 10.17221/76/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/76/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202311-0004.txt Handle: RePEc:caa:jnlvet:v:68:y:2023:i:11:id:76-2023-VETMED