Template-Type: ReDIF-Article 1.0 Author-Name: T Kimura Author-Workplace-Name: Laboratory of Small Animal Clinical Medicine, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan Author-Name: S Shimamura Author-Workplace-Name: Laboratory of Small Animal Clinical Medicine, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan Author-Name: H Sakaya Author-Workplace-Name: R&D Division, Global New Business Development Unit, Smart Appliances & Solutions BU, SHARP CORPORATION, Yao, Osaka, Japan Author-Name: T Hayashi Author-Workplace-Name: Life Innovation & Material Laboratories, Corporate Research and Development Group, SHARP CORPORATION, Tenri, Nara, Japan Author-Name: A Umemoto Author-Workplace-Name: Life Innovation & Material Laboratories, Corporate Research and Development Group, SHARP CORPORATION, Tenri, Nara, Japan Author-Name: K Sakai Author-Workplace-Name: Laboratory of Small Animal Clinical Medicine, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan Author-Name: M Yamasaki Author-Workplace-Name: Laboratory of Small Animal Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan Author-Name: T Hasegawa Author-Workplace-Name: Kakogawa Animal Hospital, Nishi-inokuchi, Higashi-kanki, Kakogawa, Hyogo, Japan Author-Name: T Shimada Author-Workplace-Name: Laboratory of Small Animal Clinical Medicine, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan Title: Evaluating the indicators of a heart rate variability analysis in dogs using Poincaré plots Abstract: Heart rate variability analyses using Poincaré plots can be useful for evaluating the autonomic nervous system function. However, the interpretation of the quantitative indicators of Poincaré plots remains controversial. Thus, few studies have verified the effectiveness of the quantitative indicators in veterinary medicine. This study aimed to verify the reliability of Poincaré plot indicators using pharmacological models in dogs. Four healthy beagles were used in this study. Each dog was treated with propranolol, atropine, and propranolol-atropine to block the sympathetic, parasympathetic, and sympathetic-parasympathetic functions, respectively. The quantitative indicators of the Poincaré plots were calculated based on data from 300 electrocardiogram beats collected before and after the administration of each drug and statistically analysed. The quantitative indicators of the Poincaré plots, such as the standard deviation perpendicular to the major axis (SD1), standard deviation along the major axis (SD2), and SD1 × SD2, significantly decreased after the drug administration in both the parasympathetic and sympathetic-parasympathetic blockade models. However, no significant differences were observed in SD1/SD2 between the groups. The Poincaré plots reflected the changes in the autonomic nervous system of dogs. In dogs, SD1, SD2, and SD1 × SD2 can detect a state in which parasympathetic nerve activity is suppressed. Keywords: canine, frequency-domain, geometric, Lorenz-plot, time-domain Journal: Veterinární medicína Pages: 42-51 Volume: 69 Issue: 2 Year: 2024 DOI: 10.17221/49/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/49/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202402-0001.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:2:id:49-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: DC Widianingrum Author-Workplace-Name: Department of Agrotechnology, Faculty of Agriculture, University of Jember, Jember, Indonesia Author-Name: DG Silaban Author-Workplace-Name: Department of Agrotechnology, Faculty of Agriculture, University of Jember, Jember, Indonesia Author-Name: WID Fanata Author-Name: H Khasanah Author-Workplace-Name: Department of Agrotechnology, Faculty of Agriculture, University of Jember, Jember, Indonesia Title: Identification of antibiotic resistance genes in Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province Abstract: Antibiotics are still used to treat mastitis in dairy cows in Indonesia. This study aimed to analyse antibiotic resistance genes in Escherichia coli (E. coli) from subclinical mastitis milk in East Java Province, Indonesia. The samples consisted of subclinical mastitis milk from cows and goats. A total of 592-quarter cow's milk and 71 goat's milk samples from both halves of the udder were collected from 67 farms in Lumajang, Banyuwangi, Malang, Sidoarjo, Jember, Pasuruan, Probolinggo, and Mojokerto. Subclinical mastitis samples were screened using the California mastitis test (CMT). E. coli was identified by phenotypic and genotypic methods. E. coli was confirmed with a primer specific to the polymerase chain reaction (PCR) technique. Gene resistance of E. coli was tested using the multiplex-PCR (mPCR) technique with primers encoding the genes temoneira enzyme (TEM), oxacillinase (OXA), sulfhydryl variable (SHV), and cefotaximase-munich IV (CTX-M IV). These genes were chosen because mastitis treatment generally uses oxacilline and β-lactam antibiotics. All data obtained were analysed descriptively. The results show that six isolates of E. coli (46.15%) carried a single resistance gene (TEM or SHV) and two isolates (33.33%) were confirmed as multiple drug-resistant organisms (MDROs) (TEM and SHV). The resistance genes were found in samples originating from Blitar, Banyuwangi, Lumajang, and Pasuruan Regencies. This research implies that antibiotic-resistance genes found in E. coli on certain farms are dangerous and may allow gene transmission to other bacteria that make treatment for mastitis or other bacterial infections ineffective. Keywords: multiple drug resistant, sulfhydryl variable (SHV), temoneira enzyme (TEM), zoonosis Journal: Veterinární medicína Pages: 35-41 Volume: 69 Issue: 2 Year: 2024 DOI: 10.17221/80/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/80/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202402-0002.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:2:id:80-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: SH Kim Author-Workplace-Name: Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Author-Name: YB Baek Author-Workplace-Name: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Author-Name: SI Park Author-Workplace-Name: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea; College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju, Republic of Korea Title: Concurrent primary splenic lymphoma and mammary gland tumour with polycystic ovaries in a dog Abstract: Here, we report a rare case of concurrent primary splenic lymphoma and mammary gland tumour (MGT) with polycystic ovaries in a 10-year-old, intact female Jindo dog. The dog was presented with multiple masses in the fourth left mammary gland, the largest of which measured 6 cm in diameter, along with enlargement of the left inguinal lymph node on physical examination. Ultrasonography, radiography, and computed tomography scans revealed polycystic ovaries and a mass in the tail of the spleen, after total splenectomy and mastectomy with ovariohysterectomy, histopathological examination identified splenic diffuse large B cell lymphoma and malignant myoepithelioma of the mammary gland was found. To our knowledge, this is the first report of the concurrent occurrence of splenic lymphoma, MGT, and polycystic ovaries in a dog. Keywords: lymphoma, mammary gland tumors, myoepithelium, polycystic ovaries, surgical resection Journal: Veterinární medicína Pages: 61-66 Volume: 69 Issue: 2 Year: 2024 DOI: 10.17221/99/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/99/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202402-0003.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:2:id:99-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R Dobsikova Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: J Blahova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: P Marsalek Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: V Doubkova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: E Zuskova Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Author-Name: J Velisek Author-Workplace-Name: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic Title: Pharmacokinetics of praziquantel and its metabolites in grass carp (Ctenopharyngodon idella) following the oral administration of a single bolus Abstract: The study aimed to evaluate and compare the routes and rates of the depletion of the antiparasitic praziquantel (PZQ), a derivative of pyrazinoisoquinoline, following its oral administration in grass carp (Ctenopharyngodon idella). We focused on the depletion of PZQ and its major metabolites - cis-hydroxy praziquantel (CPZQ) and trans-hydroxy praziquantel (TPZQ), in water, the plasma, hepatopancreas, kidney, muscle, and skin, following a single oral administration of PZQ in a concentration of 50 mg/kg. Fish were sampled before the drug administration and then eight times in the course of the 30-day-long experiment. Our results indicate the rapid absorption and elimination of PZQ and its metabolites in all the analysed matrices. The most PZQ-burdened tissue was the hepatopancreas, the gill and the skin. In all the samples, the concentration of the drug and its metabolites consistently declined over time. The residue of the parent compound was detected for the longest time in all the tissues. During the study, a significant (P < 0.01) correlation was found within the concentration of PZQ, CPZQ, and TPZQ in the water and all the biological matrices. It was also found that the PZQ residue was not detected below the maximum residue levels (i.e., 20 µg/kg) until 16 days after exposure in the muscle and skin. Keywords: fish, food safety, health, parasites Journal: Veterinární medicína Pages: 52-60 Volume: 69 Issue: 2 Year: 2024 DOI: 10.17221/109/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/109/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202402-0004.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:2:id:109-2023-VETMED