Template-Type: ReDIF-Article 1.0 Author-Name: S Nadeem Author-Workplace-Name: Institute of Microbiology, University of Agriculture Faisalabad, Faisalabad, Pakistan Author-Name: R Aslam Author-Workplace-Name: Institute of Microbiology, University of Agriculture Faisalabad, Faisalabad, Pakistan Author-Name: S Rahman Author-Workplace-Name: Institute of Microbiology, University of Agriculture Faisalabad, Faisalabad, Pakistan Author-Name: M Kasib Khan Author-Workplace-Name: Department of Parasitology, University of Agriculture Faisalabad, Faisalabad, Pakistan Title: Risk analysis and seroprevalence of bovine ephemeral fever virus in Punjab, Pakistan Abstract: Bovine ephemeral fever (BEF) is a vector-borne viral disease caused by the RNA virus which belongs to the genus Ephemerovirus and the family Rhabdoviridae. To evaluate the effect of the risk factors like the breed of cattle and buffaloes, age, sex, lactation, housing and region on the bovine ephemeral fever virus (BEFV) prevalence, ELISA and virus neutralisation (VN) tests (n = 600) were performed for the BEFV prevalence. The seroprevalence in cattle was 45.6% and 42% by ELISA and VN, respectively (P = 0.001). The breed-wise seropositive ratio was (55-64%) in cattle and (22.5-18.3%) in buffaloes by VN and ELISA. The sex-wise prevalence was (40-49.4%) in females and (35.8-46%) in males by VN and ELISA in cattle and a similar prevalence was reported in buffaloes. The age-wise prevalence in bovines by ELISA was 5.33, 22.66 and 17.66% in the age group < 1 year, 1-3 years and > 3 years, respectively. The disease prevalence was higher in the age group of 1-3 years. The prevalence was higher during the 3rd lactation in bovines. The region-wise prevalence was higher in the 07 districts while lower (18-21%) in Rawalpindi District by VN and ELISA, respectively (P = 0.001). Commercial dairy farms of cattle showed a higher disease prevalence (52% and 44%) than non-commercial farms (38% and 36%) by ELISA and VN, respectively (P = 0.227). Exotic cows showed higher disease prevalence (76.67% and 70%) by ELISA and VN. The mortality in bovines was 5% (7.7% and 2.3%) in the cattle and buffaloes. The case fatality of BEFV in bovines was 12.25%. There was a significant effect of the risk factors like the breed, age, sex, lactation, housing and region on the BEFV prevalence. This is the first comprehensive study of BEFV in Pakistan. Keywords: enzyme-linked immunosorbent assay, Ephemerovirus, lactation, Rhabdoviridae, risk factors, vector-borne, virus neutralisation (VN) Journal: Veterinární medicína Pages: 67-76 Volume: 69 Issue: 3 Year: 2024 DOI: 10.17221/95/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/95/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202403-0001.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:3:id:95-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: SH Kim Author-Workplace-Name: Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Author-Name: YB Baek Author-Workplace-Name: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea Author-Name: SI Park Author-Workplace-Name: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea; College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju, Republic of Korea Title: Canine multiple primary tumours: Mammary tubular carcinoma, uterine leiomyosarcoma, and facial sebaceous epithelioma Abstract: Multiple primary malignant tumours (MPMTs) are multiple neoplasms with independent pathogenetic origins, placing great importance on the tumorigenesis and clinical treatment. However, due to the rare occurrence and diagnostic confusion, MPMTs have rarely been investigated in veterinary medicine. In this report, a 10-year-old intact female Maltese dog had MPMTs, consisting of two malignant tumours and one benign tumour each derived from a topographically different site: tubular carcinoma in the mammary glands, leiomyosarcoma in the uterus and sebaceous epithelioma in the cheek. The unique combination of MPMTs would be the first case in veterinary research to give insight into the diagnosis, disease characteristics, and surgical treatment. Keywords: leiomyosarcoma, mammary gland tumour, multiple origins, primary tumours, sebaceous epithelioma Journal: Veterinární medicína Pages: 94-98 Volume: 69 Issue: 3 Year: 2024 DOI: 10.17221/103/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/103/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202403-0002.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:3:id:103-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Gezer Author-Workplace-Name: Vocational School of Health Services, Ataturk University, Erzurum, Turkiye Author-Name: S Yediel Aras Author-Workplace-Name: Department of Midwifery, Faculty of Health Sciences, Kafkas University, Kars, Turkiye Author-Name: N Kilic Baygutalp Author-Workplace-Name: Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkiye Author-Name: E Karadag Sari Author-Workplace-Name: Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkiye Author-Name: G Bedir Author-Workplace-Name: Department of Histology and Embryology, Ataturk University School of Medicine, Erzurum, Turkiye Author-Name: B Mokhtare Author-Workplace-Name: Department of Pathology, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkiye Author-Name: K Yilmaz Author-Workplace-Name: Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkiye Title: Effect of vitamin D3 and a stinging nettle extract on the gastric tissue of rats administered with trinitrobenzene sulfonic acid Abstract: In this study, the effects of vitamin D3 (Vit. D) and a stinging nettle (Urtica dioica L. (UD)) extract were examined using histopathological and immunohistochemical methods in the stomach tissues of an experimentally created rat model of CD. The CD model was created using trinitrobenzene sulfonic acid (TNBS). The animals in the study were divided into control, TNBS, TNBS+Vit. D, and TNBS+UD groups. At the end of the experiment, the animals were euthanised and their stomach tissues were evaluated for necrosis, degeneration, apoptosis, and inflammation. Additionally, an immunohistochemical method was applied to determine the somatostatin (SSTR), aquaporin-1 (AQP-1), caspase-3, and tumour necrosis factor-alpha (TNF-α) immunoreactivity in the gastric tissues. In the evaluations, degenerative and necrotic changes and mononuclear cell infiltration areas were observed in the TNBS group, but such changes could be improved with Vit. D and UD applications. The results suggest that the combination of the Vit. D and UD extract may have a protective and therapeutic role in mitigating TNBS-induced damage to the gastric tissues, potentially through the regulation of SSTR, AQP-1, caspase-3, and TNF-α expression. This indicates a promising avenue for further research and the exploration of these compounds in the context of gastrointestinal health. Keywords: aquaporin-1, Crohn', s disease, somatostatin, TNF-α, Journal: Veterinární medicína Pages: 84-93 Volume: 69 Issue: 3 Year: 2024 DOI: 10.17221/111/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/111/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202403-0003.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:3:id:111-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: T Karatas Author-Workplace-Name: Health Services Vocational School, Agri Ibrahim Cecen University, Agri, Turkiye Author-Name: M Cakir Author-Workplace-Name: Department of Neurosurgery, Medical Faculty, Ataturk University, Erzurum, Turkiye Title: Assessment of deltamethrin-induced DNA damage, neurotoxic and neuroimmune effects in the brain tissue of brown trout (Salmo trutta fario) Abstract: This study investigated the impact of deltamethrin (DM) toxicity on brown trout (Salmo trutta fario), examining its effects on the immune system, including the white blood cell (WBC), lymphocyte (Lym), total immunoglobulin (T. Ig), and lysozyme levels, as well as its neurotoxic consequences on the brain tissue. The neurotoxic effects encompassed oxidative stress, the activity of the antioxidant enzymes, such as the superoxide dismutase (SOD) and catalase (CAT), acetylcholinesterase (AChE) activity, and DNA damage using 8-hydroxy-2-deoxyguanosine (8-OHdG). The DM exposure led to elevated levels of malondialdehyde (MDA), and 8-OHdG, while concurrently causing a reduction in the AChE activity, protein and lipid content, WBC count, Lym, lysozyme activity, T. Ig levels, as well as the SOD and CAT levels in the brain tissues of groups 2 and 3 when compared to those in group 1. In summary, the findings of this study strongly indicate that DM induces DNA damage, immunotoxicity, and neurotoxicity in the brain tissue of brown trout, primarily due to the excessive production of reactive oxygen species (ROS). Moreover, the observed dose-dependent responses of DM to the environmental concentrations on all the investigated parameters suggest its potential utility in aquaculture risk assessment. Keywords: deltamethrin, DNA damage, fish, immunity Journal: Veterinární medicína Pages: 77-83 Volume: 69 Issue: 3 Year: 2024 DOI: 10.17221/115/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/115/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202403-0004.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:3:id:115-2023-VETMED