Template-Type: ReDIF-Article 1.0 Author-Name: E Rangel-Vargas Author-Workplace-Name: Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mexico Author-Name: CA Gomez-Aldapa Author-Workplace-Name: Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mexico Author-Name: RN Falfan-Cortes Author-Workplace-Name: Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mexico Author-Workplace-Name: Professor of the National Council of Science, Humanities and Technology, Crédito Constructor, Benito Juárez, Mexico City, CDMX Author-Name: FA Guzman-Ortiz Author-Workplace-Name: Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mexico Author-Workplace-Name: Professor of the National Council of Science, Humanities and Technology, Crédito Constructor, Benito Juárez, Mexico City, CDMX Author-Name: JC Rosas Author-Workplace-Name: Academic Area of Chemistry, Institute of Basic Sciences and Engineering, City of Knowledge, Autonomous University of the State of Hidalgo, Mineral de la Reforma, Mexico Title: A natural approach to combating antibiotic-resistant pathogens in livestock: Hibiscus sabdariffa-derived hibiscus acid as a promising solution Abstract: We examined the antibacterial efficacy of streptomycin, hibiscus acid, and their combination against multidrug-resistant Shiga-toxin-producing Escherichia coli (STEC) and Salmonella Typhimurium in mice. We determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for streptomycin, hibiscus acid, and their combination against STEC and Salmonella. Fifteen sets of six mice in each set were utilised: six groups were orally exposed to 4 log10 colony forming units (CFUs) of S. Typhimurium and another six to STEC, and three acted as the controls. Six hours post-inoculation, specific groups of mice received either oral solutions containing hibiscus acid at 5 and 7 mg/ml; streptomycin at 50 and 450 µg/ml; hibiscus acid/streptomycin (5 mg/ml hibiscus acid and 50 µg/ml streptomycin); or isotonic saline. The study determined the MIC and MBC of 7 mg/ml of hibiscus acid; 300 and 450 µg/ml of streptomycin; and two concentrations of hibiscus/streptomycin (3 mg/ml / 20 µg/ml and 5 mg/ml / 50 µg/ml). Interestingly, the mice that were infected and subsequently treated with hibiscus acid at 7 mg/ml alone or in conjunction with streptomycin did not have either STEC or Salmonella in their faecal samples, and none of the mice died. In contrast, the untreated mice and those exclusively treated with streptomycin had the pathogens present in their stool, leading to the mortality of all the subjects. Keywords: plant antimicrobial agents, Salmonella Typhimurium, Shiga-toxin-producing Escherichia coli, synergistic effect Journal: Veterinární medicína Pages: 207-216 Volume: 69 Issue: 6 Year: 2024 DOI: 10.17221/105/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/105/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202406-0001.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:6:id:105-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: T Bobic Author-Workplace-Name: Department for Animal Production and Biotechnology, Faculty of Agrobiotechnology Sciences Osijek, University of Josip Juraj Strossmayer of Osijek, Osijek, Croatia Author-Name: P Mijic Author-Workplace-Name: Department for Animal Production and Biotechnology, Faculty of Agrobiotechnology Sciences Osijek, University of Josip Juraj Strossmayer of Osijek, Osijek, Croatia Author-Name: M Gregic Author-Workplace-Name: Department for Animal Production and Biotechnology, Faculty of Agrobiotechnology Sciences Osijek, University of Josip Juraj Strossmayer of Osijek, Osijek, Croatia Author-Name: V Gantner Author-Workplace-Name: Department for Animal Production and Biotechnology, Faculty of Agrobiotechnology Sciences Osijek, University of Josip Juraj Strossmayer of Osijek, Osijek, Croatia Title: Evaluation of the hoof's temperature variations depending on lesion presence, measurement points and leg position Abstract: In order to determine the possibility of using infrared thermography (IRT) in preventing lameness in dairy cows, the aim of this study was to evaluate temperature variations depending on the lesion presence, measurement points and leg position. The study used about 3 000 IRT records from 60 Holstein cows housed in a free-stall barn. Surface temperature measurements were taken at two regions of the hooves: the region of the coronary band, and the region of the skin up to 2 cm above the coronary band. A highly significant (P = 0.004; 0.006, P < 0.01) difference in temperature was observed between healthy and diseased legs at both measurement points. Additionally, a significant (P = 0.029; 0.037; 0.045; 0.012; 0.018, P < 0.05) or highly significant (P = 0.004; 0.006, P < 0.01) difference in temperature values between the front and rear legs was established for both measurement points, i.e., the coronary band and the skin. Despite confirming the potential applicability of infrared thermography in the detection of lameness, it is crucial to consider the significant influences of the environmental factors, impurities, and animal-related factors. Keywords: coronary band, Holstein cows, skin, infrared thermography, lameness Journal: Veterinární medicína Pages: 185-190 Volume: 69 Issue: 6 Year: 2024 DOI: 10.17221/8/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202406-0002.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:6:id:8-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S Ishenbaeva Author-Workplace-Name: Department of Veterinary and Sanitary Expertise, Histology and Pathology, Kyrgyz National Agrarian University named after K.I. Skryabin, Bishkek, Kyrgyz Republic Author-Name: R Nurgaziev Author-Workplace-Name: Department of Veterinary and Sanitary Expertise, Histology and Pathology, Kyrgyz National Agrarian University named after K.I. Skryabin, Bishkek, Kyrgyz Republic Author-Name: U Tynaliev Author-Workplace-Name: Silk Road Research Center, Ala-Too International University, Bishkek, Kyrgyz Republic Author-Name: U Shergaziev Author-Workplace-Name: Department of Veterinary and Sanitary Expertise, Histology and Pathology, Kyrgyz National Agrarian University named after K.I. Skryabin, Bishkek, Kyrgyz Republic Author-Name: A Irgashev Author-Workplace-Name: Department of Veterinary and Sanitary Expertise, Histology and Pathology, Kyrgyz National Agrarian University named after K.I. Skryabin, Bishkek, Kyrgyz Republic Title: Spontaneous tumours in dogs: A clinical and pathomorphological study in Kyrgyzstan Abstract: The aim of this study is to investigate the correlation between the incidence of spontaneous tumours of various origins and the localisation in dogs with sex, breed, and age factors. A total of 360 tumours with various localisation were studied pathomorphologically. Histopathologic data sets from 360 dog tissue samples were processed and statistically examined. A chi-square test of independence was conducted to examine the relationships among the various levels of the specified variables. Logistic regression models were employed for dichotomous outcomes to ascertain the influence of certain explanatory variables on the tumour types. Characteristic pathomorphological changes observed during examination of dogs with oncologic diseases were determined. The most common neoplasms were mammary tumours, accounting for 43% of the cases. The mammary gland tumours were most common in mongrel dogs (25%), with German Shepherds (17.3%), Poodles, Dachshunds, Central Asian Shepherds (6.7% each), and Rottweilers (5.7%) following. The highest frequency of these tumours appeared at 8 years of age, predominantly originating from the ductal epithelium, which represented 46.4% of all the malignant cases. Keywords: dogs, clinical study, histopathology, localisation, tumours Journal: Veterinární medicína Pages: 198-206 Volume: 69 Issue: 6 Year: 2024 DOI: 10.17221/16/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/16/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202406-0003.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:6:id:16-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B Lipovy Author-Workplace-Name: Department of Burns and Plastic Surgery, Institution Shared with University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Workplace-Name: Advanced Biomaterials Group, Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic Author-Name: L Vacek Author-Workplace-Name: Department of Microbiology, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: D Polastik Kleknerova Author-Workplace-Name: Department of Microbiology, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: E Jeklova Author-Workplace-Name: Clinical Immunology and Immunology of Infectious Diseases, Veterinary Research Institute, Brno, Czech Republic Author-Name: L Liskova Author-Workplace-Name: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: J Holoubek Author-Workplace-Name: Department of Burns and Plastic Surgery, Institution Shared with University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: D Matyskova Author-Workplace-Name: Department of Burns and Plastic Surgery, Institution Shared with University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: F Ruzicka Author-Workplace-Name: Department of Microbiology, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic Title: Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa Abstract: Pseudomonas aeruginosa poses a significant threat to both immunocompetent and immunocompromised individuals, often resulting in life-threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for P. aeruginosa complicated skin and soft tissue infections (cSSTIs). This study presents a novel porcine model inducing and sustaining cSSTI for 14 days. Six pigs (120 wounds) were used for the development of infections, and within this group, two pigs (40 wounds) were used to evaluate the progression of the cSSTI infection. The model demonstrated bacterial loads of more than 107 CFU/gram of tissue or higher. The cSSTI fully developed within three days and remained well above these levels until day 14 post-infection. Due to the immunocompetence of this model, all the immunological processes associated with the response to the presence of infection and the wound healing process are preserved. Keywords: ESKAPE pathogen, model development, pig, wound Journal: Veterinární medicína Pages: 191-197 Volume: 69 Issue: 6 Year: 2024 DOI: 10.17221/25/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/25/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202406-0004.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:6:id:25-2024-VETMED