Template-Type: ReDIF-Article 1.0 Author-Name: Y Shi Author-Workplace-Name: College of Veterinary Medicine, Yunnan Agricultural University, Kunming, P.R. China Author-Name: D Han Author-Workplace-Name: Animal Quarantine Laboratory, Technology Center of Kunming Customs, Kunming, P.R. China Author-Name: J Li Author-Workplace-Name: Animal Quarantine Laboratory, Technology Center of Kunming Customs, Kunming, P.R. China Author-Name: L Ye Author-Workplace-Name: Animal Quarantine Laboratory, Technology Center of Kunming Customs, Kunming, P.R. China Author-Name: X Ji Author-Workplace-Name: Research Center for International Inspection and Quarantine Standard and Technical Regulation, General Administration of Customs, Beijing, P.R. China Author-Name: F Nie Author-Workplace-Name: Animal and Plant Quarantine Laboratory, Technology Center of Chongqing Customs, Chongqing, P.R. China Author-Name: Z Song Author-Workplace-Name: Research Center for International Inspection and Quarantine Standard and Technical Regulation, General Administration of Customs, Beijing, P.R. China Author-Name: C Chen Author-Workplace-Name: College of Veterinary Medicine, Yunnan Agricultural University, Kunming, P.R. China Author-Name: J Ai Author-Workplace-Name: Animal Quarantine Laboratory, Technology Center of Kunming Customs, Kunming, P.R. China Author-Name: J Xin Author-Workplace-Name: College of Veterinary Medicine, Yunnan Agricultural University, Kunming, P.R. China Title: A novel quantitative real-time PCR with the GAPDH reference gene for peste des petits ruminants Abstract: Peste des petits ruminants (PPR) is a serious acute, highly contagious disease caused by the peste des petits ruminants virus (PPRV). This study aims to establish a qRT-PCR assay with an internal amplification control for the rapid and accurate detection of PPRV. The primers and probes for PPRV N were based on the national standard of the diagnostic techniques for PPR of China, and a pair of primers and TaqMan probes for the internal reference gene of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was designed. Optimisation of the reaction conditions, specificity, sensitivity and reproducibility tests, and clinical sample detection were conducted. The results showed that the optimal primers and probe concentrations of PPRV were 0.4 μmol/l and 0.4 μmol/l, respectively, and were 0.4 μmol/l and 0.2 μmol/l for the reference gene GAPDH, respectively. The established method has no cross-reaction with other viruses. The minimum detection limit was 6.8 copies/µl for PPRV and 190 copies/µl for GAPDH. The coefficients of variation (CV%) of PPRV and GAPDH were both lower than 2%. The results suggest that the PPRV qRT-PCR method containing internal reference genes has strong specificity, high sensitivity, and good reproducibility. The addition of internal reference genes for the sample quality control improves the accuracy of the detection. Keywords: detection method, fluorescence real-time quantitative PCR, internal reference gene, peste des petits ruminants virus Journal: Veterinární medicína Pages: 234-242 Volume: 69 Issue: 7 Year: 2024 DOI: 10.17221/123/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/123/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202407-0001.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:7:id:123-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: JH Jung Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: H Park Author-Workplace-Name: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: WS Kim Author-Workplace-Name: Department of Anatomy, College of Veterinary Medicine, and Veterinary Science Research Institute, Konkuk University, Seoul, Republic of Korea Author-Name: HY Yoon Author-Workplace-Name: Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Workplace-Name: KU Center for Animal Blood Medical Science, Konkuk University, Seoul, Republic of Korea Title: Comprehensive comparative study of Chiari-like malformation in veterinary and human medicine Abstract: This review aims to enrich our understanding of Chiari-like malformation (CLM) by combining human and veterinary insights, and providing a detailed cross-species overview. CLM is a developmental abnormality characterised by caudal displacement of the hindbrain into the foramen magnum due to an entire brain parenchymal shift caused by insufficient skull volume. This malformation leads to a progressive obstruction at the craniocervical junction, which disrupts the normal cerebrospinal fluid flow, leading to secondary syringomyelia. The clinical signs of CLM and syringomyelia include phantom scratching, head tilt, head tremor, ataxia, tetraparesis, pain, muscle atrophy, and scoliosis or torticollis. Magnetic resonance imaging remains the gold standard for diagnosing CLM, since it allows the visualisation of abnormal findings such as the caudal cerebellar herniation, caudal cerebellar compression from occipital dysplasia, and attenuated cerebrospinal fluid cisternae. Although various medical and surgical interventions, including foramen magnum decompression, can provide temporary symptomatic/clinical sign relief, current literature shows a lack of sustained long-term efficacy. Therefore, additional research is needed to evaluate the long-term effects of existing treatment strategies and to compare different techniques utilised in conjunction with foramen magnum decompression. Keywords: Chiari-like malformation, Chiari type 1 malformation, cranioplasty, duraplasty, foramen magnum decompression, syringomyelia Journal: Veterinární medicína Pages: 217-233 Volume: 69 Issue: 7 Year: 2024 DOI: 10.17221/125/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/125/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202407-0002.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:7:id:125-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Castanho Author-Workplace-Name: Faculty of Veterinary Medicine, University Lusófona - Lisbon University Center, Campo Grande, Lisbon, Portugal Author-Name: J Catarino Author-Workplace-Name: Faculty of Veterinary Medicine, University Lusófona - Lisbon University Center, Campo Grande, Lisbon, Portugal Author-Workplace-Name: School of Animal Health, Protection and Welfare, Lusophone Polytechnic Institute, Lisbon, Portugal Author-Name: L Pedroso Author-Workplace-Name: Faculty of Veterinary Medicine, University Lusófona - Lisbon University Center, Campo Grande, Lisbon, Portugal Author-Workplace-Name: Veterinary and Animal Research Centre (CECAV), University Lusófona - Lisbon University Center, Campo Grande, Lisbon, Portugal Author-Name: A Alvarado Author-Workplace-Name: Department of Veterinary Medicine, University of Évora, Évora, Portugal Author-Workplace-Name: Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB) University of Trás-os-Montes and Alto-Douro, Vila Real, Portugal Author-Name: S Ramos Author-Workplace-Name: Faculty of Veterinary Medicine, University Lusófona - Lisbon University Center, Campo Grande, Lisbon, Portugal Author-Workplace-Name: Veterinary and Animal Research Centre (CECAV), University Lusófona - Lisbon University Center, Campo Grande, Lisbon, Portugal Title: Melanocytic cutaneous lesions in Sus ibericus, are these a cause for meat condemnation? Abstract: The Alentejano pig (Sus ibericus) is an autochthonous breed of swine from Portugal phylogenetically close to the Iberian breed that is known to develop melanocytic lesions. In this study, 34 melanocytic skin lesions were identified and collected from Alentejano pigs slaughtered for human consumption for further routine histologic assessment. The samples were classified into 4 age ranges: 1 (1 to 6 months), 2 (7 to 12 months) 3 (13 to 24 months), and 4 (more than 25 months). All the lesions were considered benign after the histopathological assessment, of which 52.9% and 47.1%, were classified as melanosis and melanocytomas, respectively. Regarding the age ranges, a statistical difference between the groups was observed, indicating that the probability of melanosis presentation was higher at the age range 4 and for melanocytomas at the age range 3. While no malignant lesions were observed in this study, it was concluded that benign melanocytic lesions are commonly found in Alentejano pig carcasses. Further research is necessary to accurately distinguish between malignant and benign lesions, which is crucial for official veterinarians to make decisions regarding meat approval or condemnation. Keywords: Alentejano pig, meat safety, melanocytic tumours, melanocytoma, melanosis Journal: Veterinární medicína Pages: 254-259 Volume: 69 Issue: 7 Year: 2024 DOI: 10.17221/5/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202407-0003.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:7:id:5-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Gulaydin Author-Workplace-Name: Department of Surgery, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkiye Author-Name: O Gulaydin Author-Name: MB Akgul Author-Workplace-Name: Department of Surgery, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkiye Title: Isolation of aerobic bacteria from surgical site infections following orthopaedic operations in cats and dogs Abstract: Surgical site infections (SSIs) cause significant complications in the postoperative period in veterinary surgeries. Determining the aetiology of infections is crucial for increasing treatment success rates. This study aims to assess treatment processes by identifying the bacterial agents responsible for infections occurring in cats and dogs after orthopaedic operations and to determine the antimicrobial resistance profiles of these agents. Strains isolated from SSIs were retrospectively analysed in patients brought to the Surgical Clinic of Siirt University Animal Health Application and Research Hospital between 2021 and 2023. The isolates were identified using MALDI-TOF MS. The disc diffusion method was applied to determine the antimicrobial susceptibility of the isolates. A high isolation rate was detected in methicillin-resistant Staphylococcus pseudintermedius and extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae. According to the antimicrobial susceptibility results, cephalosporin treatment was continued in only one of the cases in which prophylactic treatment with cephalosporin group antibiotics was applied. Consequently, this study revealed that preoperative prophylactic antibiotic administration may not be sufficient in preventing surgical site infections. Diagnosis of aetiological agents and evaluation of antimicrobial susceptibility are essential in preventing surgical site infections and determining effective treatment options. Keywords: antimicrobial resistance, cat, dog, Staphylococcus pseudintermedius, surgery Journal: Veterinární medicína Pages: 243-253 Volume: 69 Issue: 7 Year: 2024 DOI: 10.17221/26/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/26/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202407-0004.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:7:id:26-2024-VETMED