Template-Type: ReDIF-Article 1.0 Author-Name: J Toholova Author-Workplace-Name: Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic Author-Name: S Hornak Author-Workplace-Name: Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic Author-Name: M Kuricova Author-Workplace-Name: Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic Title: Non-surgical pain management for hip joint disease in veterinary medicine Abstract: The most common orthopaedic developmental disease in dogs is hip dysplasia. This condition results in coxofemoral laxity due to incongruity and lack of stabilisation of the joint by the soft tissues. Currently, there is no therapeutic plan to correct hip dysplasia without surgical intervention at a very early age. The goal of the non-surgical treatment is to relieve pain and stiffness and to increase the muscle strength, usually through hydrotherapy and the beneficial physical properties of water. Recently, there has been growing interest in regenerative medicine, which involves the use of mesenchymal stem cells (MSCs) and their products to alleviate the characteristic clinical symptoms of osteoarthritis (OA). In vivo studies with canine MSCs have shown that an intra-articular injection of MSCs into cartilage lesions leads to the excellent regeneration of the hyaline cartilage. Regenerative medicine has undergone rapid development in recent years thanks to new therapies based on the application and combination of innovative biomaterials. One of the first known regenerative methods to be used in clinical practice was platelet-rich plasma (PRP). This review summarises the use and potential of MSCs and PRP, including their in vitro properties, their therapeutic effects in the treatment of cartilage lesions in preclinical in vivo studies, their clinical efficacy in the treatment of naturally occurring OA in dogs, and the current limitations of the studies. Keywords: hip dysplasia, mesenchymal stem cells, platelet-rich plasma, regenerative medicine Journal: Veterinární medicína Pages: 261-272 Volume: 69 Issue: 8 Year: 2024 DOI: 10.17221/19/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/19/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202408-0001.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:8:id:19-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: GD Akarsu Author-Workplace-Name: Laboratory for Advanced Genomics, Division of Molecular Medicine, Institute of Ruder Boskovic, Zagreb, Croatia Author-Workplace-Name: Pharmacy Services, Vocational School of Health Services, Yozgat Bozok University, Yozgat, Turkiye Author-Name: R Hobek Akarsu Author-Workplace-Name: Department of Nursing, Faculty of Health Services, Yozgat Bozok University, Yozgat, Turkiye Title: Therapeutic potential of cannabis for surgical wound healing in rats Abstract: This study was conducted to evaluate the wound-healing activities of a Cannabis sativa L. plant extract and cannabidiol on incision wounds. An incision was created and sutured in rats under anaesthesia. Routine wound care procedures were applied for 10 days, followed by histological wound examinations. The cellular bioactivities of the hemp extract and CBD were assessed for MCP-1, EGF, BFGF, IL-8, and COL-1 using ELISA on the rat skin wound healing activity. A one-way ANOVA was used for the data analysis. The EGF values in the plasma were similar in the povidone-iodine, hemp seed oil, and hemp essential oil groups (P > 0.05). However, the EGF levels were lower in the CBD group compared to the other groups (P < 0.001, P < 0.005). The MCP-1 values in the hemp seed oil, hemp essential oil, and CBD were similar (P > 0.05), whereas povidone iodine exhibited lower MCP-1 levels compared to the other groups (P < 0.001, P < 0.005). It was determined that the plasma BFGF, IL-8, and COL 1 values of the groups were similar (P > 0.05). To our knowledge, this study is the first to evaluate the effects of CBD, seed oil, and hemp leaf extract on incision wound healing. It demonstrates that hemp extract holds greater potential benefits for wound healing compared to CBD. Keywords: cannabinoids, cannabis sativa, essential oil, hemp, treatment, wounds and injury Journal: Veterinární medicína Pages: 297-306 Volume: 69 Issue: 8 Year: 2024 DOI: 10.17221/21/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/21/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202408-0002.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:8:id:21-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S Kovarikova Author-Workplace-Name: Department of Animal Welfare and Protection and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic Author-Name: J Blahova Author-Workplace-Name: Department of Animal Welfare and Protection and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic Author-Name: I Vanova Author-Workplace-Name: Department of Physiology, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic Author-Name: P Marsalek Author-Workplace-Name: Department of Animal Welfare and Protection and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic Title: Comparison of two methods of measuring the urinary protein concentration for the determination of the urinary protein to creatinine ratio in various animal species Abstract: Determination of the urinary protein-to-creatinine ratio (UPC) is an important tool in the quantification of proteinuria in animals. However, the result may be affected by the different methods of determining the urinary protein concentration. The aim of this study was to compare the turbidimetric method using benzethonium chloride and the colorimetric method using pyrogallol red in the measurement of the urinary protein concentration in dogs, cats, guinea pigs and horses. A total of 464, 192, 216 and 119 urine samples from dogs, cats, guinea pigs and horses were examined in the study, respectively. The group consisted of animals of both sexes and different ages, and, in the dogs and cats, it included both healthy animals and those with various health problems. In the group of horses and guinea pigs, only clinically healthy animals were included. A total of 347, 185, 103 and 100 samples from the dogs, cats, guinea pigs and horses were used in the statistical analysis; the other values were excluded as they were below the detection limit. According to the Passing-Bablok analysis, there was a significant constant and proportional difference in the horses. In the dogs, cats and guinea pigs, there was a significant constant difference, but no proportional difference. The Bland-Altman method showed significant bias between the two methods in the horses and cats, but not in the dogs and guinea pigs. In the dogs and cats, the agreement between the two methods was tested and expressed as Cohen's kappa (κ). In the cats, it was almost perfect for the proteinuric samples (κ = 0.823 3) and significant for the non-proteinuric samples (κ = 0.804 9). In the dogs, the agreement was significant for the non-proteinuric samples (κ = 0.621 5) and only moderate for the proteinuric samples (κ = 0.527 5). The influence of the method used to determine the urinary protein concentration should be taken into account when evaluating the UPC. Repeated examinations in one patient should be performed with the same method. Keywords: cat, dog, guinea pig, horse, proteinuria, UPC Journal: Veterinární medicína Pages: 273-280 Volume: 69 Issue: 8 Year: 2024 DOI: 10.17221/30/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/30/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202408-0003.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:8:id:30-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I Kostovova Author-Workplace-Name: Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic Author-Name: K Kavanova Author-Workplace-Name: Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic Author-Name: M Moravkova Author-Workplace-Name: Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic Author-Name: J Gebauer Author-Workplace-Name: Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: L Leva Author-Workplace-Name: Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: M Vícenova Author-Workplace-Name: Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: V Babak Author-Workplace-Name: Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic Author-Name: M Faldyna Author-Workplace-Name: Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: M Crhanova Author-Workplace-Name: Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic Title: Probiotic bacteria of wild boar origin intended for piglets - An in vitro study Abstract: Using probiotics represents a potential solution to post-weaning diarrheal diseases in piglets on commercial farms. The gastrointestinal tract of wild boars serves as a promising reservoir of novel lactic acid bacteria with suitable probiotic characteristics. In this study, we isolated eight bacterial strains from the intestinal content of wild boars identified as representatives of the species Bifidobacterium apri, Lactobacillus amylovorus, and Ligilactobacillus salivarius. These isolates underwent in vitro analysis and characterisation to assess their biological safety and probiotic properties. Analysis of their full genome sequences revealed the absence of horizontally transferrable genes for antibiotic resistance. However, seven out of eight isolates harboured genes encoding various types of bacteriocins in their genomes, and bacteriocin production was further confirmed by mass spectrometry analysis. Most of the tested strains demonstrated the ability to inhibit the growth of selected pathogenic bacteria, produce exopolysaccharides, and stimulate the expression of interleukin-10 in porcine macrophages. These characteristics deem the isolates characterised in this study as potential candidates for use as probiotics for piglets during the post-weaning period. Keywords: antibiotic susceptibility, antimicrobial activity, bacteriocins, exopolysaccharides, interleukin-10 Journal: Veterinární medicína Pages: 281-296 Volume: 69 Issue: 8 Year: 2024 DOI: 10.17221/35/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/35/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202408-0004.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:8:id:35-2024-VETMED