Template-Type: ReDIF-Article 1.0 Author-Name: GS Kumar Author-Workplace-Name: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India Author-Name: A Varghese Author-Workplace-Name: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India Author-Name: PK Hembram Author-Workplace-Name: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India Author-Name: KGA Kumar Author-Workplace-Name: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India Author-Name: CK Deepa Author-Workplace-Name: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India Author-Name: A Varghese Author-Workplace-Name: Veterinary Consultant, Zootopia Veterinary Clinic, Chalakudy, Kerala, India Author-Name: R Ravindran Author-Workplace-Name: Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India Title: Molecular detection and characterisation of Mycoplasma species in community owned dogs of Kerala, a South Indian State Abstract: Haemotropic mycoplasmas (haemoplasmas) are obligate epierythrocytic bacteria that infect a wide range of vertebrate hosts. The molecular characterisation of Mycoplasma organisms in dogs has not previously been attempted from India. Hence, in the present study, the molecular characterisation of Mycoplasma spp. in dogs of different zones of Kerala was attempted using 16S rRNA gene. An overall prevalence of 18% for M. haemocanis was detected. The NCBI-BLAST analysis of all the selected sequences revealed > 99% identity with the sequences of M. haemocanis. The phylogenetic analysis revealed clustering of M. haemocanis and M. haemofelis in a single clade indicating low genetic variability. It was further supported by the genetic distance data and haplotype analysis. Keywords: 16S rRNA, dogs, haplotype networks, Mycoplasma haemocanis, phylogenetic analysis Journal: Veterinární medicína Pages: 321-328 Volume: 69 Issue: 9 Year: 2024 DOI: 10.17221/116/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/116/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202409-0001.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:9:id:116-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M Zedda Author-Workplace-Name: Department of Veterinary Medicine, University of Sassari, Sassari, Italy Author-Name: R Babosova Author-Workplace-Name: Department of Zoology and Anthropology, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic Author-Name: S Gadau Author-Workplace-Name: Department of Veterinary Medicine, University of Sassari, Sassari, Italy Author-Name: G Lepore Author-Workplace-Name: Department of Veterinary Medicine, University of Sassari, Sassari, Italy Author-Name: S Succu Author-Workplace-Name: Department of Veterinary Medicine, University of Sassari, Sassari, Italy Author-Name: V Farina Author-Workplace-Name: Department of Veterinary Medicine, University of Sassari, Sassari, Italy Title: Does a relation between bone histomorphometry and fractures exist? The case of the equine radius and tibia Abstract: Fractures of long bones in limbs are rare traumatic events in horses. This study investigates whether the incidence and types of fractures can be related to the histomorphometric features of the radius and tibia, which experience different biomechanical stresses and exhibit varying incidences and types of fractures. Clinical observations suggest that, in adults, slightly transverse and comminuted fractures are present in the radius, while the tibia shows a higher frequency of longitudinal and spiral fractures. Microscopic observations reveal no apparent distinctive characteristics between the radius and tibia, whereas the histomorphometric data highlight differences in the osteon density, eccentricity, and diameters of the osteons and Haversian canals. To sum up, tibial osteons are more numerous and smaller than those in the radius, resulting in a 15% higher total extension of the cement line in the tibia compared to the radius. These histomorphometric differences are an evolutionary adaptation to the different biomechanical stresses that involve the thoracic and pelvic limbs. Our results could help better understand numerous clinical realities detectable through retrospective analyses and aid in evaluating a specific bone's predisposition towards traumatic events in all mammals, including humans. Keywords: bone tissue, Haversian canals, horse, osteons, traumatic events Journal: Veterinární medicína Pages: 307-313 Volume: 69 Issue: 9 Year: 2024 DOI: 10.17221/18/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/18/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202409-0002.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:9:id:18-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A Kopecka Author-Workplace-Name: Equine Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: T Novotna Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: Z Svobodova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: Z Drabkova Author-Workplace-Name: Equine Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Title: Senecio ovatus poisoning in a horse - A case report Abstract: This study describes a case of poisoning by pyrrolizidine alkaloids in a horse. To the best of the author's knowledge, this is the first confirmed case of Senecio ovatus poisoning. A six-year-old 450-kg Irish cob mare was presented to the Equine Clinic of the University of Veterinary Sciences Brno (Czechia) with symptoms of hepatic encephalopathy, which progressively worsened with time despite intensive therapy and led to euthanasia. A complex diagnostic and therapeutic approach including the post-mortem patoanatomical and histopathological examination is described here. Regarding the histopathology of the liver, there was necrosis with haemorrhage, fatty changes and inflammation. A later inspection of the grazing area revealed the presence of Senecio ovatus (wood ragwort). A sensitive chromatographic method was used to determine the pyrrolizidine alkaloids and their metabolites in the plasma and the liver. In both of the samples, metabolites of pyrrolizidine alkaloids were detected. Although pyrrolizidine alkaloid poisoning was proven, the histopathological findings typical for this disease were absent. It is clear from our case that the histopathology in cases of poisoning by pyrrolizidine alkaloids may not always be conclusive. Keywords: equine, histopathology, chromatography, intoxication, pyrrolizidine alkaloid, wood ragwort Journal: Veterinární medicína Pages: 329-336 Volume: 69 Issue: 9 Year: 2024 DOI: 10.17221/37/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/37/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202409-0003.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:9:id:37-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z Svobodova Author-Workplace-Name: Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: J Drapal Author-Workplace-Name: Central Veterinary Administration of the State Veterinary Administration, Prague, Czech Republic Author-Name: V Vlasakova Author-Workplace-Name: Central Veterinary Administration of the State Veterinary Administration, Prague, Czech Republic Author-Name: D Harustiakova Author-Workplace-Name: Faculty of Science, RECETOX, Masaryk University, Brno, Czech Republic Author-Name: J Illek Author-Workplace-Name: Large Animal Clinical Laboratory, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: M Svoboda Author-Workplace-Name: Ruminant and Swine Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic Title: A multi-year study monitoring the cadmium content in the tissues of lambs and sheep sampled in the Czech Republic between 2001 and 2022 Abstract: Cadmium is one of the most important environmental contaminants. Animals grazing on natural pastures are particularly exposed to cadmium. Sheep are mostly reared in extensive and grazing systems. Therefore, sheep may accumulate larger amounts of cadmium compared to other ruminant species and are a good indicator of exposure to cadmium contamination in a given area. The determination of cadmium concentrations in the muscles, livers and kidneys of lambs and sheep was carried out in the Czech Republic during the period 2001-2022. The average cadmium content in the livers and kidneys of all the lambs was 0.037 ± 0.006 and 0.061 ± 0.013 mg.kg-1, respectively. The average cadmium content in the livers and kidneys of all the sheep was 0.319 ± 0.047 and 1.255 ± 0.204 mg.kg-1, respectively. The maximum limit for human consumption was not exceeded in any of the 24 samples in the lambs but was exceeded in 5 of 33 liver samples and 12 of 33 kidney samples in the adult sheep. The average value of cadmium in the muscles of the lambs and sheep was 0.004 ± 0.001 mg.kg-1. The maximum limit for human consumption was not exceeded in any muscle sample. The cadmium content in both the liver and in the kidney differs significantly between the lambs and sheep, being higher in the sheep (P < 0.001 for both comparisons). No statistically significant trend of a decrease in the cadmium content in the lamb and sheep tissues was found during the observed time (P > 0.05). We can sum up that there is an evident need for further monitoring of the cadmium concentration in lamb and sheep tissues in the Czech Republic. Keywords: heavy metal, kidney, lamb, liver, sheep Journal: Veterinární medicína Pages: 314-320 Volume: 69 Issue: 9 Year: 2024 DOI: 10.17221/45/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/45/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202409-0004.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:9:id:45-2024-VETMED