Template-Type: ReDIF-Article 1.0 Author-Name: S Maresova Author-Workplace-Name: Small Animal Cinic, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: T Liptak Author-Workplace-Name: Small Animal Cinic, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: A Madari Author-Workplace-Name: Small Animal Cinic, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: P Zeleznik Author-Workplace-Name: Small Animal Cinic, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: C Tothova Author-Workplace-Name: Small Animal Cinic, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Author-Name: M Kuricova Author-Workplace-Name: Small Animal Cinic, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic Title: Evaluation of CTX-I, CTX-II, TIMP-I, MMP-9 and PIICP in the population of working German Shepherd dogs in Slovakia Abstract: The aim of this study was to determine the reference values of cartilage damage biomarkers in the blood and urine in 76 clinically healthy German Shepherd dogs. We grouped the dogs into 4 groups by age. All the groups were consistently made up of 19 dogs. The mean age and average body weight were established for all the dog groups. The blood was collected from the vena cephalica antebrachii or the vena saphena medialis and the urine sample was taken by us-guided cystocenthesis. The biomarkers were determined using quantitative sandwich ELISA kits. The mean values of the biomarkers ± SD were, for the urine biomarkers, CTX-I 3.29 ± 1.16 ng/ml, CTX-II 1 993.95 ± 777.04 ng/ml, and TIMP-I 392.80 ± 160.56 ng/ml, and for the blood biomarkers, MMP-9 89.85 ± 50.21 ng/ml and PIICP 19.19 ± 7.33 ng/ml. Based on the obtained values of the mean 95%, we expect a standard for CTX-I 5.05 ng/ml, CTX-II 3 204.26 ng/ml, TIMP-I 606.64 ng/ml, MMP-9 187.93 ng/ml, and PIICP 31.71 ng/ml. Keywords: blood, cartilage, C-terminal telopeptides, metalloproteinase, urine Journal: Veterinární medicína Pages: 355-361 Volume: 69 Issue: 10 Year: 2024 DOI: 10.17221/127/2023-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/127/2023-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202410-0001.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:10:id:127-2023-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N Marecakova Author-Workplace-Name: Small Animal Clinic, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: J Kacirova Author-Workplace-Name: Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Nitra, Slovak Republic Author-Name: C Tothova Author-Workplace-Name: Clinic of Ruminants, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: A Madari Author-Workplace-Name: Small Animal Clinic, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: M Madar Author-Workplace-Name: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: M Kuricova Author-Workplace-Name: Small Animal Clinic, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Author-Name: S Hornak Author-Workplace-Name: Small Animal Clinic, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic Title: N-acetyl-β-D-glucosaminidase in clinically healthy German Shepherd dogs and dogs with early stages of chronic kidney disease Abstract: Urinary N-acetyl-β-d-glucosaminidase (NAG) activity is associated with kidney disease. In our study, we focused on evaluating the ratio of NAG to the urinary creatinine concentration (NAG/Cr) in the German Shepherd breed. Sixty-two healthy dogs and thirteen dogs with chronic kidney disease (CKD) were examined. The healthy dogs were divided into groups based on their sex and age (puppies and adolescent dogs under 2 years, adult dogs from 2 to 6 years and seniors over 6 years), while the dogs with CKD were divided based on the disease stage (CKD stage 1 and 2). No significant difference was detected between the males and females. Regarding the age dependence, significantly higher values were found in dogs older than 6 years (P = 0.020 3) compared to dogs aged 2-6 years. When comparing the healthy dogs to the dogs with CKD, the lowest NAG/Cr ratio was observed in the dogs with CKD stage 2. The reference interval for NAG/Cr in the German Shepherd was established in the range of 0.78-7.86 U/gCr. We observed no correlations with the commonly used markers of kidney disease such as creatinine, urea, or symmetrical dimethylarginine. Nevertheless, we encourage the further investigation of NAG in relation to CKD, taking the breed, sex and age of the patients into account. Keywords: enzyme activity, kidney disease, urinalysis, urine enzyme, tubular marker Journal: Veterinární medicína Pages: 337-344 Volume: 69 Issue: 10 Year: 2024 DOI: 10.17221/34/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/34/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202410-0002.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:10:id:34-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M Makovicka Author-Workplace-Name: Department of Histology and Embryology, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic Author-Workplace-Name: Cancer Research Institute, Biomedical Research Centre of the Slovak Academy of Sciences, Bratislava, Slovak Republic Author-Name: P Bolgac Author-Workplace-Name: Department of Pathology, Veterinary and Food Institute, Bratislava, Slovak Republic Author-Name: K Kajo Author-Workplace-Name: Department of Pathology, St. Elisabeth Cancer Institute, Bratislava, Slovak Republic Author-Name: P Makovicky Author-Workplace-Name: Department of Histology and Embryology, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic Author-Workplace-Name: Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic Author-Name: P Svorc Author-Workplace-Name: Institute of Physiology and Pathophysiology, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic Title: An unusual finding of schwannoma in the mammary gland of a dog Abstract: Peripheral nerve sheath tumours (PNSTs) are rare in the mammary glands of dogs. Here, we report a case of a schwannoma, composed of two parts, of the mammary gland of a dog. The first part consists of clusters of uncircumscribed, alternating, more concentrated and looser regions. In the more concentrated parts, typical fascicularly arranged schwannoma intermingle in plexiform arrangement, more subtle in appearance than in neuronal tumour areas. The second part undergoes regression consisting predominantly of residual shorter rosettes of cells with the presence of a peculiar variably sized swirling of target-like formations consisting of compact, thicker, pinkish lamellae also with occasional adjacent cells. Immunohistochemically, the tumour cells are positive for evidence of vimentin and neuro-specific enolase. They exhibit the variable expression of the S-100 protein, show mild CD56 positivity, and focally mildly accentuated proliferative activity as assessed by Ki-67. The tumour elements are negative for evidence of cytokeratin 7, cytokeratin 20, and oestrogen receptors. Hybrid tumours may change their morphology in combination with atypical localisation and may be underdiagnosed in veterinary biopsy practice. They differ from epithelial tumours prognostically, as well as in their development and behaviour, therefore it is essential to clearly differentiate them. Keywords: histopathology, hybrid tumours, mammary gland, perineural cells, peripheral nerve cells, Schwann cells, veterinary oncology Journal: Veterinární medicína Pages: 362-368 Volume: 69 Issue: 10 Year: 2024 DOI: 10.17221/41/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/41/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202410-0003.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:10:id:41-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O Eralp Inan Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Eskisehir Osmangazi Univesity, Eskisehir, Turkey Author-Name: P Levent Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey Author-Name: A Saril Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey Author-Name: L Hamabe Author-Workplace-Name: Department of Veterinary Medicine, Facility of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan Author-Name: M Kocaturk Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey Author-Name: Z Yilmaz Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey Title: Evaluation of coagulation by thromboelastography and a velocity curve in dogs with parvoviral enteritis Abstract: Canine parvoviral enteritis (CPE) has a high mortality rate in untreated dogs due to systemic inflammation and multi-organ dysfunction. The inflammatory process can lead to coagulation abnormalities. This study aimed to evaluate the coagulation status using thromboelastography (TEG) and assess the thrombin generation (TG) and clot dissolution using TEG-derived velocity curve (v-curve) parameters in dogs with CPE. It included 21 dogs with CPE and five healthy dogs. In addition to the clinico-haemato-biochemical examinations, the coagulation status was analysed using citrated venous blood samples with TEG. All the dogs with CPE met at least two criteria for systemic inflammatory response syndrome (SIRS). The comparison to healthy controls showed a statistically significant prolongation of reaction times (R time; P = 0.005) and times to the maximum rate of thrombus generation (TMRTG; P = 0.003). However, the times to the maximum rate of lysis (TMRL; P = 0.041) and total lysis (L; P = 0.024) decreased significantly. The TEG tracings showed coagulation states varying from hypocoagulation to hypercoagulation in dogs with CPE. These results showed that the v-curve derivate can be used to evaluate the coagulation in dogs with CPE, and it could be superior to the standard TEG variables for determining the low fibrinolytic activity. Thus, the v-curve parameters may provide a novel insight into the underlying mechanism and clinical treatment strategy of CPE-induced inflammation. Keywords: coagulation, fibrinolysis, hypercoagulation, hypocoagulation, inflammation, sepsis Journal: Veterinární medicína Pages: 345-354 Volume: 69 Issue: 10 Year: 2024 DOI: 10.17221/49/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/49/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202410-0004.txt Handle: RePEc:caa:jnlvet:v:69:y:2024:i:10:id:49-2024-VETMED