Template-Type: ReDIF-Article 1.0 Author-Name: K Raulinaite Author-Workplace-Name: Department of Anatomy and Physiology, Faculty of Veterinary, Veterinary Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: R Zelvyte Author-Workplace-Name: Department of Anatomy and Physiology, Faculty of Veterinary, Veterinary Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: K Skemiene Author-Workplace-Name: Laboratory of Biochemistry, Neuroscience Institute, Lithuanian University of Health Sciences, Kaunas, Lithuania Author-Name: I Monkeviciene Author-Workplace-Name: Department of Anatomy and Physiology, Faculty of Veterinary, Veterinary Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania Title: Treatment tactic of canine cranial cruciate ligament rupture management: A 28-day comparative analysis of ACP and NSAID induced effects on the serum MMP-3 levels and clinical outcomes Abstract: Cranial cruciate ligament rupture (CrCLR) is a common stifle joint pathology among dogs, leading to osteoarthritis and painfulness. Non-surgical treatment options often represent the usage of non-steroidal anti-inflammatory drugs for 14 days (NSAIDs), but autologous conditioned plasma (ACP) shows promising results in managing various orthopaedic conditions, decreasing inflammation, and improving the clinical outcome in dogs. This study aimed to determine the differences in MMP-3 serum levels and the clinical outcomes between differently treated cranial cruciate rupture cases. For this purpose, we used two different treatment methods for managing canine cranial cruciate ligament rupture (minimally invasive ACP injection or oral NSAIDs), and evaluated the clinical outcomes, indicating the quality of life, and the MMP-3 serum levels over a period of 28 days. The findings of this investigation indicate that ACP has better efficacy than two weeks of NSAIDs in inflammation reduction, clinical outcome improvement, and the allowance of a longer duration of activity after 28 days. Keywords: autologous conditioned plasma, dog, cranial cruciate ligament rupture, functional outcomes, matrix metalloproteinase 3, nonsteroidal anti-inflammatory drugs Journal: Veterinární medicína Pages: 124-133 Volume: 70 Issue: 4 Year: 2025 DOI: 10.17221/39/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/39/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202504-0001.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:4:id:39-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N Suwan Author-Workplace-Name: Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand Author-Name: S Jenjittikul Author-Workplace-Name: Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand Author-Name: R Tiratrakoonseree Author-Workplace-Name: Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand Author-Name: C Jiradanaipat Author-Workplace-Name: Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand Author-Name: S Teerawongsuwan Author-Workplace-Name: Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand Author-Name: Wi Phathomrapeepong Author-Workplace-Name: Wipawadee animal clinic, Bangkok, Thailand Author-Name: W Phanwanich Author-Workplace-Name: Department of Biomedical Engineering, Faculty of Engineer, Mahidol University, Nakhon Pathom, Thailand Author-Workplace-Name: Petaneer Co., Ltd. Salaya, Nakhon Pathom, Thailand Author-Name: R Rungsiwiwut Author-Workplace-Name: Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand Title: Allogeneic adipose MSCs and autologous PRP for chronic non-healing wound in a cat Abstract: Management of chronic non-healing wounds in cats requires a comprehensive approach. This report describes the treatment of a severe open skin wound on the skull using a combined approach involving allogeneic adipose-derived mesenchymal stem cells (MSCs) and autologous platelet-rich plasma (PRP). A 12-year-old neutered male mixed-breed domestic cat presented with a non-healing chronic wound on the skull. The wound extended from the orbital to the occipital area and from the left to the right temporal region. Laboratory test results were positive for feline immunodeficiency virus and impaired kidney function. Sensitivity tests revealed resistance to several antibiotics. Due to limited skin reconstruction options, MSCs were administered subcutaneously at the wound edge once a month for three months. PRP was collected one month after the initial MSC administration and injected at the wound edge monthly between MSC treatments. The wound diameter was measured daily during saline cleaning. The cat received protein-supplemented food daily. Wound healing was observed two weeks after the MSC administration, gradually decreasing in size and closing completely within 5 months. This case demonstrates the successful application of MSCs and PRP for treating chronic wounds in cats. Keywords: bite wound, regenerative veterinary medicine, stem cells Journal: Veterinární medicína Pages: 143-149 Volume: 70 Issue: 4 Year: 2025 DOI: 10.17221/93/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/93/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202504-0002.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:4:id:93-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: JS Batista Author-Workplace-Name: Department of Agrarian Sciences, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro, RN, Brazil Author-Name: TS Teofilo Author-Workplace-Name: Department of Agrarian Sciences, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro, RN, Brazil Author-Name: HN Araujo Junior Author-Workplace-Name: Center for Education, Science and Technology of the Inhamuns Region, State University of Ceará, Taua, CE, Brazil Author-Name: NS Felix Author-Workplace-Name: Department of Agrarian Sciences, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro, RN, Brazil Author-Name: KAR Paiva Author-Workplace-Name: Department of Agrarian Sciences, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro, RN, Brazil Author-Name: TA Neves Author-Workplace-Name: Department of Agrarian Sciences, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro, RN, Brazil Author-Name: LS Reboucas Author-Workplace-Name: Department of Agrarian Sciences, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro, RN, Brazil Author-Name: GVD Medeiros Author-Workplace-Name: Department of Agrarian Sciences, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro, RN, Brazil Author-Name: KMFM Costa Author-Workplace-Name: Department of Agrarian Sciences, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro, RN, Brazil Author-Name: FAF Xavier Junior Author-Workplace-Name: Center for Education, Science and Technology of the Inhamuns Region, State University of Ceará, Taua, CE, Brazil Author-Name: G Negri Author-Workplace-Name: Department of Botany, University of Sao Paulo, Sao Paulo, SP, Brazil Author-Name: A Salatino Author-Workplace-Name: Department of Botany, University of Sao Paulo, Sao Paulo, SP, Brazil Author-Name: CEP Jara Author-Workplace-Name: Department of Botany, University of Sao Paulo, Sao Paulo, SP, Brazil Title: Gastroprotective effect of the hydroethanolic extract of geopropolis produced by Melipona subnitida (Meliponinae, Apidae) in Wistar rats Abstract: The jandaíra bee (Melipona subnitida) is a species native to northeastern Brazil that produces geopropolis, a mixture of clay and propolis, used in folk medicine. Geopropolis has traditionally been used in folk medicine because of its potential therapeutic properties, including antimicrobial, anti-inflammatory, and wound-healing effects. Recent studies have highlighted the rich composition of bioactive compounds such as flavonoids and phenolic acids, contributing to their pharmacological potential. Despite these findings, the gastroprotective properties of geopropolis and the underlying mechanisms remain underexplored and warrant further investigation. Therefore, we aimed to evaluate the gastroprotective effects of a hydroethanolic extract of geopropolis (HEG) produced by M. subnitida in Wistar rats, focusing on its antioxidant activity and the role of its bioactive compounds in preventing gastric mucosal damage. The gastroprotective potential was evaluated in Wistar rats pre-treated with HEG (250, 500, and 1 000 mg/kg, orally) for seven days and subjected to acute gastric lesions with ethanol (0.2 ml/animal, orally). One group of rats that received only distilled water served as the negative control, whereas the other group that received only ethanol served as the positive control. The stomachs were evaluated to determine the following parameters: evidence of macroscopic and histological changes, volume of mucus-containing mucin, stomach pH, and index of ulcerative lesions. Kolmogorov-Smirnov and Levene tests were performed, followed by the Tukey test, with values considered significant at P < 0.05. HEG reduced the severity of the ulcerative lesions at all doses tested. Additionally, there was no significant difference in the pH values of gastric secretions, mucus volume, and mucin content in the stomachs of animals pretreated with HEG compared to the negative control group. These results indicate that HEG has gastroprotective activity, which may be related to the presence of phenolic compounds and its high antioxidant activity. Keywords: gastric ulcers, gastroprotective, geopropolis, phenolic compounds, stingless bees Journal: Veterinární medicína Pages: 134-142 Volume: 70 Issue: 4 Year: 2025 DOI: 10.17221/98/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/98/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202504-0003.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:4:id:98-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N Mazreku Author-Workplace-Name: Department of Veterinary Medicine, Faculty of Agriculture and Veterinary, University of Prishtina "Hasan Prishtina", Prishtina, Republic of Kosovo Author-Name: D Sylejmani Author-Workplace-Name: Department of Veterinary Medicine, Faculty of Agriculture and Veterinary, University of Prishtina "Hasan Prishtina", Prishtina, Republic of Kosovo Author-Name: A Robaj Author-Workplace-Name: Department of Veterinary Medicine, Faculty of Agriculture and Veterinary, University of Prishtina "Hasan Prishtina", Prishtina, Republic of Kosovo Title: Prevalence of subclinical mastitis, related risk factors, and antimicrobial susceptibility of bacteria isolated from the milk of dairy cows in Kosovo Abstract: The purpose of this study was to determine the prevalence and risk factors associated with subclinical mastitis, as well as the antimicrobial susceptibility of bacteria isolated from the milk of dairy cows in the Republic of Kosovo. The study involved 242 lactating cows from 16 farms. Data were collected through questionnaire interviews, the California mastitis test (CMT), and microbiological analysis. For the detection and identification of bacteria, conventional methods and biochemical tests were used. The disc diffusion method was used to test the susceptibility of isolated bacteria. The prevalence of subclinical mastitis (SCM) at quarter and cow level was 22.3% and 10.5%, respectively. Factors including breed, age, parity, milk production, and lactation stage were significantly correlated with the occurrence of subclinical mastitis in cows (P < 0.05). Major bacterial isolates were Staphylococcus aureus (34%), coagulase-negative staphylococci (CNS, 21.3%), Escherichia coli (18.1%), Streptococcus uberis (14.9%), and Streptococcus agalactiae (4.3%). All bacterial isolates showed high susceptibility to gentamicin, tetracycline, amoxicillin/clavulanic acid, and sulfamethoxazole/trimethoprim and low susceptibility to penicillin and streptomycin. Keywords: antibiotic, disc diffusion, pathogen, sensitive Journal: Veterinární medicína Pages: 117-123 Volume: 70 Issue: 4 Year: 2025 DOI: 10.17221/102/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/102/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202504-0004.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:4:id:102-2024-VETMED