Template-Type: ReDIF-Article 1.0 Author-Name: L Cermak Author-Workplace-Name: Department of Physiology of Institute of Animal Science, Nutrition and Quality of Animal Products, Prague, Czech Republic Author-Name: E Pechouckova Author-Workplace-Name: Department of Physiology of Institute of Animal Science, Nutrition and Quality of Animal Products, Prague, Czech Republic Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic Author-Name: M Marounek Author-Workplace-Name: Department of Physiology of Institute of Animal Science, Nutrition and Quality of Animal Products, Prague, Czech Republic Author-Name: T Paulova Author-Workplace-Name: Department of Physiology of Institute of Animal Science, Nutrition and Quality of Animal Products, Prague, Czech Republic Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic Title: Pathogenic bacteria in biogas plants using cattle, swine, and poultry manure Abstract: Fugate, a waste product from biogas production, regularly used in agriculture as a fertiliser, may contain bacterial pathogens that cause zoonoses. Anaerobic digestion (AD) can inactivate viable pathogens, including parasites, viruses, and pathogens containing antibiotic resistance genes. This study aimed to compare the numbers of pathogenic bacteria and diversity of potential bacterial pathogens in the fugate using three different types of slurry: cattle, swine, and poultry manure. The swine fugate showed higher numbers of Clostridium perfringens and Campylobacter sp. than the poultry and cattle fugate. In the cattle fugate, the lowest total number of pathogenic bacteria and a low number of coliforms were detected after the AD. The use of cattle manure in biogas plants presents a lower potential for soil contamination with pathogens. The fugate produced using poultry or swine manure can be used carefully to avoid possibility of contamination of aquifers or surface waters. Also fugate produced from manure of cows suffering from chronic botulism  can be used only with carefulness because of the presence of Clostridium botulinum spores in biogas waste of diseased cows. Keywords: anaerobic digestion, animal slurry, bacterial pathogens, fugate Journal: Veterinární medicína Pages: 151-155 Volume: 70 Issue: 5 Year: 2025 DOI: 10.17221/47/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/47/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202505-0001.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:5:id:47-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F Liao Author-Workplace-Name: Guizhou Vocational College of Agriculture, Qingzhen, P.R. China Author-Name: J He Author-Workplace-Name: Guizhou Vocational College of Agriculture, Qingzhen, P.R. China Title: Antibacterial activity and compositional analysis of Acer truncatum bunge leaf extract against Staphylococcus aureus Abstract: Staphylococcus aureus is a zoonotic pathogen that can cause various diseases in both humans and animals. This experiment evaluates the antibacterial activity of extracts from Acer truncatum leaves against S. aureus, including the minimum inhibitory concentration (MIC), growth curve, and cell membrane integrity assessment, alongside the identification of the extract components by LC-MS. Results demonstrated that the MIC of the n-butanol extract from the ethanol extract of Acer truncatum leaves against S. aureus was 3.125 mg/ml, with a minimum bactericidal concentration (MBC) of 6.25 mg/ml. Over 16 h, the extracts at concentrations of 0.25 MIC, 0.5 MIC, and 1 MIC effectively inhibited S. aureus growth. The fluorescence staining revealed that the extracts at different concentrations compromised the structural integrity of the cell membrane. The LC-MS analysis identified the principal constituents of the extract as betaine (27.189%), 3,4-dihydroxymandelic acid (16.112%), quercitrin (14.768%), chlorogenic acid (8.778%) and neochlorogenic acid (4.452%). The study indicated that Acer truncatum leaf extract has good potential for application in natural antibacterial drugs. Keywords: antimicrobial agents, cell membrane integrity, LC-MS analysis, phenolic compounds Journal: Veterinární medicína Pages: 166-176 Volume: 70 Issue: 5 Year: 2025 DOI: 10.17221/65/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/65/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202505-0002.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:5:id:65-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: MJ Bartolome Author-Workplace-Name: Livestock Research and Development Division, Bureau of Animal Industry, Department of Agriculture, Visayas Avenue, Diliman, Quezon City, Philippines Author-Name: LAM Aguirre Author-Workplace-Name: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna, Philippines Author-Name: CM Poliquit Author-Workplace-Name: Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Banos, College, Laguna, Philippines Author-Name: I Besas Author-Workplace-Name: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna, Philippines Author-Name: JG Angeles Author-Workplace-Name: Philippine Genome Center - Program for Agriculture, Livestock, Forestry and Fisheries, University of the Philippines Los Baños, Los Baños, Laguna, Philippines Author-Name: J Rabajante Author-Workplace-Name: Institute of Mathematical Sciences and Physics, College of Arts and Sciences, University of the Philippines Los Baños, College, Laguna, Philippines Author-Name: S Camba Author-Workplace-Name: Institute of Animal Science, College of Agriculture and Food Sciences (CAFS), University of the Philippines Los Baños, College, Laguna, Philippines Author-Name: F Del Valle Author-Workplace-Name: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna, Philippines Author-Name: A Ambrocio Author-Workplace-Name: Office of the Provincial Veterinarian, Provincial Government of Quezon, Pagbilao, Quezon, Philippines Author-Name: F Caguicla Author-Workplace-Name: Office of the Provincial Veterinarian, Provincial Government of Quezon, Pagbilao, Quezon, Philippines Author-Name: MG Bustamante Author-Workplace-Name: Office of the Provincial Veterinarian, Provincial Government of Laguna, Santa Cruz, Laguna, Philippines Author-Name: D Umali Author-Workplace-Name: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna, Philippines Title: Environmental DNA (eDNA) contamination patterns of African swine fever virus (ASFV) in swine transport vehicles in the Philippines Abstract: Since its introduction in 2019, African swine fever (ASF) has spread to all regions of the Philippines, affecting 73 out of its 82 provinces. To assess the environmental DNA (eDNA) contamination patterns of the ASF virus (ASFV) in swine transport vehicles and evaluate its measures of association, a total of 450 environmental swabs from 30 transportation vehicles were tested using qPCR. Five out of 30 vehicles (16.67%) tested positive in at least one of the following areas: cargo area or sidecar walls (6.67%), cargo area or sidecar floors (6.67%), hauling personnel's hands (6.67%), steering wheel or handlebars (3.33%), gear shift levers (3.33%), floor mats or footpegs (3.33%), dashboards (3.33%), door handles or sidecar gate bolts (3.33%), tyres/wheels (3.33%), fenders (3.33%), and hauling personnel's footwear (3.33%). All investigated risk factors were analysed using Chi-square, Fisher's exact test, and odds ratio and were found to be insignificant, including the frequency of swine transportation per week, frequency of cleaning per week, cleaning materials used, disinfection practices, the number of pigs transported, and whether hauliers owned pigs (p > 0.05). This study illuminates the environmental contamination patterns of ASFV in swine transport vehicles, underscoring the need for targeted biosecurity protocols in areas with a high risk of contamination, particularly the cabin areas. More effective vehicle disinfection systems that will not damage equipment or pose health risks to hauliers are recommended to reduce the risk of ASF disease transmission between countries and geographic regions. Keywords: African swine fever, biosecurity, environmental surveillance, fomites, qPCR Journal: Veterinární medicína Pages: 156-165 Volume: 70 Issue: 5 Year: 2025 DOI: 10.17221/84/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/84/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202505-0003.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:5:id:84-2024-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: TU Kim Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: JS Park Author-Workplace-Name: Gumi Top Animal Medical Center, Gumi, Republic of Korea Author-Name: JH Yim Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: WJ Kim Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: SB Hong Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Name: YR Jung Author-Workplace-Name: Daegu Health College, Department of Companion Animal Health Management, Daegu, Republic of Korea Author-Name: SK Choi Author-Workplace-Name: Core Protein Resources Center, Daegu-Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea Author-Name: SM Baek Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Institute for Veterinary Biomedical Science, Kyungpook National University, Daegu, Republic of Korea Author-Name: JK Park Author-Workplace-Name: Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea Author-Workplace-Name: Institute for Veterinary Biomedical Science, Kyungpook National University, Daegu, Republic of Korea Title: Uterine angiomyofibroblastoma in a domestic cat: A need of precise diagnosis for proper perioperative management Abstract: Angiomyofibroblastoma (AMFB) is rarely reported in humans as well as domestic animals, especially in the female genital tract. This painless tumour is characterised by the proliferation of spindle or ovoid tumour cells with myofibroblastic differentiation, which often cluster around thin-walled blood vessels. This report presents a case of feline AMFB for the first time. Preoperative abdominal radiography demonstrated an enlarged uterus with the dorsolateral displacement of the ovaries, and transabdominal ultrasonography showed an enlarged uterus with diffuse hypoechoic areas. The resected uterus showed a firm texture with a grey to brownish colour. A microscopic examination revealed proliferation of well-differentiated neoplastic spindle cells on a background of abundant fibrous stroma containing numerous blood vessels. Myxoid degeneration and vascular extravasation were not observed. The neoplastic cells were diffusely immunopositive for α-SMA and vimentin and focally positive for desmin (in the perivascular areas). AMFB is rare in veterinary medicine and the feline uterine AMFB observed in the present case had not been previously reported. Although previously reported as a safe, benign tumour, a surgical procedure of a large AMFB in small animals without proper diagnosis may become life-threatening. Keywords: α, -SMA, desmin, feline, hemangiopericytoma, uterus Journal: Veterinární medicína Pages: 177-183 Volume: 70 Issue: 5 Year: 2025 DOI: 10.17221/88/2024-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/88/2024-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202505-0004.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:5:id:88-2024-VETMED