Template-Type: ReDIF-Article 1.0 Author-Name: O Gulaydin Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkiye Author-Name: M Yesilyurt Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkiye Author-Name: G Akgul Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkiye Author-Name: O Erdeger Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkiye Author-Name: K Eercan Author-Workplace-Name: Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkiye Title: Haemotropic Mycoplasma species in cat blood samples by PCR Abstract: Haemotropic mycoplasmas can cause severe anaemia in pets and also have zoonotic potential. The determination of these microorganisms from clinical samples is of critical importance. In this study, the presence of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm), and Candidatus Mycoplasma turicensis (CMt) in blood samples collected from 62 cats was investigated. Haemotropic mycoplasmas were identified by PCR amplifying the 16S rRNA gene, and sequence analysis was applied for confirmation and differentiation of the species. Six (9.67%) blood samples were positive for haemotropic mycoplasmas. CMhm and CMt were identified in five (8.06%) samples and one (1.61%) sample, respectively. Mhf was not detected in the samples. Additionally, a co-infection was not observed in any of the cats. It was found that the PCR positivity was not related to the sex or clinical status of the cats. To our knowledge, this is the first report investigating haemotropic mycoplasmas in blood samples from cats in Siirt Province of Türkiye. It is suggested that the data obtained from this study will contribute to clinicians working on pet animal health in this province. Keywords: Candidatus Mycoplasma haemominutum, feline, feline haemoplasma, molecular detection Journal: Veterinární medicína Pages: 294-301 Volume: 70 Issue: 8 Year: 2025 DOI: 10.17221/7/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202508-0001.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:8:id:7-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: G Sonmez Author-Workplace-Name: Department of Genetics, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkiye Author-Name: MC Temizkan Author-Workplace-Name: Department of Laboratory and Veterinary Health, Yozgat Bozok University, Yozgat, Turkiye Author-Name: MH Culha Author-Workplace-Name: Department of Genetics, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkiye Title: SOD1 and CAT gene expressions in stray and owned animals Abstract: Dogs and cats are the most common companion animals in urban environments. Some dogs and cats live in stable home settings, receiving regular care. However, stray dogs and cats are exposed to chronic stress due to life on the streets or in shelters. Stress is correlated with oxidative stress. The dysregulation of redox balance can lead to the accumulation of reactive oxygen species, which promote cellular and molecular damage. In this study, the blood samples were collected from 150 female animals (90 dogs and 60 cats) to isolate peripheral blood mononuclear cells (PBMCs). Total RNA was extracted from PBMCs and subjected to reverse transcription. The expression levels of SOD1 and CAT were quantified using quantitative real-time polymerase chain reaction. Compared with those in stray animals, the SOD1 expression levels were significantly higher (P < 0.01), and the CAT expression levels were non-significantly lower in owned animals. These findings suggest that the expression of antioxidant defence genes varied between owned and stray animals. Thus, oxidative stress regulation is dependent on environmental conditions and lifestyle in companion species. Keywords: antioxidant, Canis lupus familiaris, Felis catus, gene expression Journal: Veterinární medicína Pages: 273-278 Volume: 70 Issue: 8 Year: 2025 DOI: 10.17221/12/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/12/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202508-0002.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:8:id:12-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Y Sung Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: J Park Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: Y Chae Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: T Yun Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: BT Kang Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Author-Name: H Kim Author-Workplace-Name: Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea Title: Conduction block and thin and hypokinetic myocardial segments in feline hypertrophic cardiomyopathy Abstract: A 12-year-old castrated male domestic shorthair cat was referred for respiratory distress. Physical examination revealed a systolic heart murmur at the left apex and crackles in all lung fields. Thoracic radiography showed Valentine-shaped cardiomegaly, pulmonary oedema, and pleural effusion. Echocardiography revealed focal thickening of the interventricular septum [11.01 mm; reference interval (RI) = 3.00-5.20 mm] and left ventricular posterior wall (7.41 mm; RI = 3.00-5.10 mm) during diastole. In the apex region, the free wall was focally thinned to approximately 1.6 mm with hypokinetic myocardial movement, indicating thin and hypokinetic myocardial segments. Additionally, decreased left atrial fractional shortening (12.5%; RI = 23.9-34.9%) and an increased left atrial-to-aortic ratio (2.87; RI = 0.88-1.43) were observed, along with spontaneous echocardiographic contrast in the left atrium, indicating increased thrombotic risk. The electrocardiogram showed a left axis deviation with small R waves and deep S waves in lead II, which is consistent with a left anterior fascicular block caused by delayed conduction in the left anterior fascicle. This case report describes the coexistence of a left anterior fascicular block and thin, hypokinetic myocardial segments in feline hypertrophic cardiomyopathy, suggesting a possible pathophysiological link. Keywords: electrocardiogram, feline, fibrosis, myocardial remodelling Journal: Veterinární medicína Pages: 302-306 Volume: 70 Issue: 8 Year: 2025 DOI: 10.17221/14/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/14/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202508-0003.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:8:id:14-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: GS Ekren Asici Author-Workplace-Name: Department of Biochemistry, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, Turkiye Author-Name: F Kiral Author-Workplace-Name: Department of Biochemistry, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, Turkiye Author-Name: A Bildik Author-Workplace-Name: Department of Biochemistry, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, Turkiye Author-Name: PA Ulutas Author-Workplace-Name: Department of Biochemistry, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, Turkiye Title: Cytotoxic and apoptotic effects of etoposide and ellagic acid alone or with possible synergistic/additive combinations on a canine D-17 osteosarcoma cell line Abstract: Osteosarcoma (OSA) is the most prevalent and aggressive malignancy of canine bones. Etoposide is an effective chemotherapeutic agent for cancer treatment, whereas ellagic acid is a naturally occurring compound with antioxidant and anticancer properties; however, both are inhibitors of the topoisomerase enzyme. In this study, the synergistic/additive effect of etoposide, known to have a growth-inhibitory impact in canine osteosarcoma (OSA) cells, and ellagic acid was investigated. The mechanism by which this effect inhibits cell growth at lower etoposide doses was also examined. The IC₅₀ values of both agents were determined, and possible combination doses were generated accordingly and applied to canine OSA cells. The apoptotic effects of the combinations were evaluated based on DNA breaks and the activity levels of caspase 3, 8, and 9. These findings were supported by the expression levels of Bcl-2, Bax, and Bid genes, as well as the AO/EtBr staining method. The effects on cell cycle and proliferation were analysed through survivin and NF-κβ gene expressions. Antimetastatic effects were determined using invasion and migration assays. EA is a potential therapeutic agent for cancer treatment. In combination with ET, a higher anticancer efficacy was demonstrated compared to etoposide alone. Potential treatment side effects can be reduced by enabling the use of lower drug doses. Keywords: acridine orange/ethidium bromide staining, Bax/Bcl-2 ratio, caspase , 3, 8, 9 Journal: Veterinární medicína Pages: 279-293 Volume: 70 Issue: 8 Year: 2025 DOI: 10.17221/24/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/24/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202508-0004.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:8:id:24-2025-VETMED