Template-Type: ReDIF-Article 1.0 Author-Name: A Ramesova Author-Workplace-Name: Department of Physiology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: K Machackova Author-Workplace-Name: Department of Physiology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: I Vanova Author-Workplace-Name: Department of Physiology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Workplace-Name: Small Animal Clinical Laboratory, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: M Lacinova Author-Workplace-Name: Department of Physiology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: A Bartoskova Author-Workplace-Name: Department of Reproduction, Small Animal Clinic, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: R Novotny Author-Workplace-Name: Department of Reproduction, Small Animal Clinic, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: R Vitasek Author-Workplace-Name: Department of Reproduction, Small Animal Clinic, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: K Rehakova Author-Workplace-Name: Department of Physiology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Workplace-Name: Small Animal Clinical Laboratory, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: J Doubek Author-Workplace-Name: Department of Physiology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Workplace-Name: Small Animal Clinical Laboratory, University of Veterinary Sciences Brno, Brno, Czech Republic Title: Coagulation profile in bitches with pyometra: Standard tests and thromboelastography Abstract: This study aimed to determine coagulation changes in bitches with pyometra based on a series of coagulation tests and thromboelastography (TEG), and to assess the incidence of disseminated intravascular coagulation (DIC). Eighteen bitches with pyometra and thirty-four control bitches were examined. Haematological, biochemical, and following haemostasis parameters were measured, including: prothrombin time (PT), activated partial thromboplastin time (aPTT), antithrombin activity (AT), and levels of fibrinogen (FBG), d-dimers (DD), tissue factor (TF), plasminogen (PLG), tissue plasminogen activator inhibitor 1 (TPAI-1), and thromboelastography. DIC was considered present if three or more of these parameters were abnormal: platelet count (<153 × 109/l), PT (>8.1 s), aPTT (>25.5 s), FBG (<0.6 g/l), DD (>0.2 mg/l), and AT (<107%). Significant differences were found in the PT, aPTT, FBG, DD, TPAI-1, clotting time, α-angle, and maximal amplitude. According to our scoring system, two patients were DIC positive. The study found alterations in several coagulation tests and hypercoagulable TEG tracings in bitches with pyometra, which point to excessive activation of coagulation, delayed fibrinolysis, and the presence of DIC. No patient bled abnormally, which may suggest that DIC is not overt in the majority of pyometra patients. Keywords: antithrombin, disseminated intravascular coagulation, dog, haemostasis, sepsis Journal: Veterinární medicína Pages: 408-416 Volume: 70 Issue: 11 Year: 2025 DOI: 10.17221/16/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/16/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202511-0001.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:11:id:16-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P Trampus Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: A Csagola Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: T Szalai Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: N Palmai Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: A Toth Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: N Terenyi Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: Z Nagy Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: NA Szeplaki Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: G Somogyi Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: E Rausch Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: Z Penzes Author-Workplace-Name: Ceva-Phylaxia Zrt., Budapest, Hungary Author-Name: R Krejci Author-Workplace-Name: Ceva Santé Animale, Libourne Cedex, France Title: Efficacy of a new ready-to-use PCV2 and Mycoplasma hyopneumoniae vaccine under field conditions Abstract: Coinfection of porcine circovirus type 2 (PCV2) with Mycoplasma hyopneumoniae (M. hyo) causes major worldwide economic losses within the swine industry. This study aimed to assess the safety and efficacy of a single dose of a bivalent vaccine containing PCV2d and M. hyo antigen (Cirbloc® M Hyo) under field conditions. Two studies were performed under the GCP (Good Clinical Practice) requirements on farrow-to-finish farms in Hungary and Cyprus. On both farms, the presence of both PCV2 and M. hyo infection was demonstrated. For both studies, safety parameters were observed and measured from inclusion at 21 (±3) days of age until 14 days after vaccination. Efficacy parameters were observed and measured from inclusion until slaughter. Administration of the vaccine was safe in both studies, as no general, immediate, or local reactions were observed. The efficacy of the vaccine was confirmed in both studies as the following parameters were significantly reduced in the vaccinated groups compared to the control groups: viraemia, faecal shedding, viral load in lungs and in all collected lymphoid tissues, M. hyo-specific lung lesions, and average daily body weight gain. These results collectively support the vaccine's potential as an effective tool for disease control. Keywords: bivalent, immunisation, PCV2d, PRDC, trial, swine Journal: Veterinární medicína Pages: 401-407 Volume: 70 Issue: 11 Year: 2025 DOI: 10.17221/25/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/25/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202511-0002.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:11:id:25-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J Prucha Author-Workplace-Name: Department of Communication and Information Technologies, Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic Author-Name: J Skopalik Author-Workplace-Name: Department of Communication and Information Technologies, Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic Author-Name: T Parak Author-Workplace-Name: Laboratory Medicine Centre, Trauma Hospital of Brno, Brno, Czech Republic Author-Workplace-Name: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic Author-Name: P Bratka Author-Workplace-Name: Department of Communication and Information Technologies, Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic Author-Name: J Cuprova Author-Workplace-Name: Department of Healthcare and Population Protection, Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic Title: Experimental ultrasound stimulator for improving the diffusion of exosomes and drugs into lung tissue Abstract: Pulmonary fibrosis is not only a consequence of the recent COVID-19 pandemic, but is increasingly recognised by both human and veterinary healthcare providers. Pulmonary fibrosis is a progressive condition that leads to a decline in respiratory function and even death. In this work, we compared MSC-derived exosomes with conventional anti-inflammatory drug treatments. Exosomes from stimulated MSCs displayed higher miRNA concentrations (in particular, miRNA-30b was significantly increased). A set of rats with induced lung fibrosis were divided into four groups: NC (control - no treatment), A2 (exosome infusion), A3 (exosome infusion combined with ultrasound stimulation), and F1 (tamoxifen/metformin drug treatment). The rats' lungs were subjected to histological analysis; the fibrosis scores for groups F1 and A3 were very similar and decreased significantly compared with group NC. Ultrasound-facilitated diffusion of exosomes from the capillaries into the lung tissue could represent an innovative therapeutic approach for slowing fibrosis and prolonging the active life of the organism. Keywords: drug infusion, electromagnetic stimulus, exosomes, fibrosis, therapeutic ultrasound Journal: Veterinární medicína Pages: 417-426 Volume: 70 Issue: 11 Year: 2025 DOI: 10.17221/41/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/41/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202511-0003.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:11:id:41-2025-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z Krepelkova Author-Workplace-Name: Clinic of Swine, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: K Bardova Author-Workplace-Name: Clinic of Swine, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: F Zigo Author-Workplace-Name: Department of Animal Nutrition and Husbandry, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Author-Name: A Csorgo Author-Workplace-Name: Private Veterinarian, ARTVET, Gabcikovo, Slovak Republic Author-Name: J Novotny Author-Workplace-Name: Clinic of Swine, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic Title: Seasonal dynamics and nutritional risk factors of gastric ulcers in fattening pigs: Results from a one-year field study in Slovakia Abstract: This study aimed to comprehensively evaluate the prevalence, severity, and risk factors associated with gastric lesions in fattening pigs across all four seasons in Slovakia. A total of 1 944 porcine stomachs were examined post-mortem at commercial slaughterhouses, focusing on the non-glandular region (pars oesophagea). A macroscopic evaluation was conducted using a standardised scoring system (0-3), in which gastric lesions, including parakeratosis, erosions, and ulcerations, were observed in 48% of the examined stomachs. Significant seasonal variation was detected, with the highest lesion prevalence recorded during the summer months, likely due to heat stress and reduced feed intake, and the lowest incidence of pathological changes seen in autumn. The gastric fullness had a notable impact: empty and liquid-filled stomachs were more frequently associated with severe mucosal damage, while full stomachs exhibited a protective effect. Furthermore, the feeding regimen played a crucial role: the pigs receiving wet feed had a significantly lower prevalence of gastric lesions than those on a dry feeding regimen. These results underscore the multifactorial nature of gastric ulceration in pigs and highlight the importance of nutritional and environmental management strategies in intensive production systems. Keywords: gastric ulcers, prevalence, seasonal variability, stomach content, swine Journal: Veterinární medicína Pages: 393-400 Volume: 70 Issue: 11 Year: 2025 DOI: 10.17221/44/2025-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/44/2025-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-202511-0004.txt Handle: RePEc:caa:jnlvet:v:70:y:2025:i:11:id:44-2025-VETMED